LOCUS       CP052543                3511 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0141 plasmid pB16KP0141-6,
            complete sequence.
ACCESSION   CP052543
VERSION     CP052543.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609869
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:16:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,124
            CDSs (total)                      :: 6,005
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 71 of 164
            Pseudo Genes (incomplete)         :: 88 of 164
            Pseudo Genes (internal stop)      :: 30 of 164
            Pseudo Genes (multiple problems)  :: 23 of 164
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0141"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0141-6"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(194..505)
                     /locus_tag="HJW81_30595"
     CDS             complement(194..505)
                     /locus_tag="HJW81_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM13690.1"
                     /translation="MRRAEMAYERGGDFLEDARKILNREAIGPRRSRFSIGSAPLTSIT
                     KSDAQISGGETRQDYKDTRRFPLAAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            790..1131
                     /locus_tag="HJW81_30600"
     CDS             790..1131
                     /locus_tag="HJW81_30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJM13689.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1070..1828
                     /locus_tag="HJW81_30605"
                     /pseudo=""
     CDS             1070..1828
                     /locus_tag="HJW81_30605"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1809..2306
                     /locus_tag="HJW81_30610"
     CDS             1809..2306
                     /locus_tag="HJW81_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJM13686.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            2313..2528
                     /gene="mbeD"
                     /locus_tag="HJW81_30615"
     CDS             2313..2528
                     /gene="mbeD"
                     /locus_tag="HJW81_30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJM13687.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mbeD"
     gene            2923..3105
                     /locus_tag="HJW81_30620"
     CDS             2923..3105
                     /locus_tag="HJW81_30620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM13688.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          532..705
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(44..565)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1122..2644
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtagccgca gtcaggccca tacttcaaga cctctgaatc tctctctcgt atatcccagt
       61 taccagtggc tgctgccagt ggcgctttgt cgtgctttct cgggttggac tcaagatgat
      121 agttaccggg taaggcgcag cagtcggact gaacgggggg tttgtgcata cagtccagct
      181 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat
      241 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
      301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
      361 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg
      421 gccctatcgc ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
      481 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
      541 aagcggaata tatcctgtat cacatattct gctgacgcgc cggtgccgcc ttttttctcc
      601 tgccacatga agcacttcac tgacatcctc atccgtgcca acatagtaag ccagtataca
      661 ctccgctagc gctacgtgac tggttcaggg ctgcgccccg aaaccccact gacgcgcctg
      721 cggcttgtcc aaccccgcgc cgaccgggaa aggctttccc gtccgtcacg gcgttatcag
      781 gaggcgattc tggcagataa acgcaacaag atgctcacga tgtgggtgac ggaagacgaa
      841 caccggcggt tgcttgagcg gtgcgacgga aaacaactgg cggcgtggat gcgccagacc
      901 tgccttgatg agaaaccgac acgcgccggc aggcttccct cgatttcgcc cgcgctgctg
      961 cgtcagcttg ccggcatggg caataacctg aaccagatcg cccggaaagt gaacgccggt
     1021 ggtgccggtc atgaccgggt gcagatcgtg gcagcgctga tggcgatcga tgccggtctg
     1081 gaacggttgc ggcatgccgt actggaaaaa gggccggacg atgattgtta aattccatcc
     1141 ccggggtcgc ggcgggggcg ccggtcctgt ggattatctt ctgggtaaag accggcagcg
     1201 tgaaggtgcc agcgtcctgc aggggaagcc ggaagaagtc cgggagctga ttgatgcgtc
     1261 gccgtatgtg aaaaaatata cctccggggt gctgtcattc gcggaggcgg acctgccgcc
     1321 gggtcagcgt gaaaaactga tggccagctt tgagcgggtt ctgatgcccg gactcgataa
     1381 agaccagtac agcattctgt gggtggaaca cgcagacaag ggtcgtcttg agctgaattt
     1441 tctgatcccc aataccgaat tactgacggg gaaacgcctc cagccgtatt acgatcgtgc
     1501 cgaccggccg cgcatcgatg cgtggcagac agtggtcaac ggcaggctgg ggctacatga
     1561 tccgaacgac ccggagaacc ggcgggcgct ggtcacgcct tcaggactgc cagaaacgaa
     1621 gcaggaagcc gcagagacga ttacacgggg tttgctgatg ttagcctcat caggagagct
     1681 gaaaacgcgt gatgacgtca caggggcgct cacagcggcc ggttttgagg tggtcagaac
     1741 cacaaaaaac agtatcagca ttgccgaccc ggacgggggg cgaaatatcc gactgagggg
     1801 agccatttat gagcagtctt ttaacggcct cgagggactt cgaggagaaa tcgaaagcac
     1861 agcagcagct taccgaagag aggctgaaag ccgcattcag caagcacgag aacgttgtca
     1921 aatcggaact gaacgcaagc gcgaagagaa tcaacgacgc catcagcgcc cacgaacagg
     1981 ggatgaacgc ggcgatgcag tcgaaccggc tgagcgtgct gcgaatggtc gggcggacgt
     2041 ggctgaccat caccatggtg tcagggctgc tgttcgccag cctgagcggg gtgctgtggt
     2101 accaggggaa ccggatagcg gcgaacctgg cggaaatcag gcagcagaga gatacgctgt
     2161 cgaaactgca catgcagacg tggggcgtga cctatctgga gaaccgcaac ggacggtttc
     2221 tggtgctgcc ggaggggatg aaagccgaaa cgggccggac ggtggacaac aagacgcgga
     2281 acgcggtgaa gctggtacgg gagtagagca gaatgacgga actggaaaaa caattgctga
     2341 gcgcgttaga gcagctacag caggactact cgagaaggct ggacgagtgg gagagcgcct
     2401 tcgaggaatg gcggaagatg tctggtctta tgcaacggga gaacgcggcg ctgagcgagc
     2461 gcgtgtcggg cttgagcagg caggtgcaga gtttgagcga gcagctgcgc cggttatcga
     2521 caggctgaat gagattgagc aacagcgata cgggcaaaca acggaaaagg aaccagagaa
     2581 gacagcagat ctggtaccgg aacggcaacg cacattatct catggtgaca gggagtatgg
     2641 agactgataa acgcccgtct caggtggcct gagcgtgacg gggatcatgg ccattttctg
     2701 cgatttcagc acacagagag tcttttttaa ccgaaatcac cggctatgca gtgatgtgaa
     2761 taagtgatgc atatgcgtaa aataaatcag gtgcgtttct ggtggttttc ggtgtacttg
     2821 ccaccacctt ttgcccgtca gccatcagaa acgccctgag tgcctccggc gcggtgcggg
     2881 taagagatgt tatgtttaat gttgataaca ggagtaagag tgatgcttac atttattggt
     2941 gtagtggtag tcgccgttat tgcatttaaa ttatttatgc ttttttttag ttcatccgaa
     3001 tctgccgtcg aacgtgctac ccgtaaatat atggagaacc ctacgagtgc taactataaa
     3061 ataatggtgg ctacacgcat gagaaatgag aagaaaagag ggtgatatat gtttgagttt
     3121 attggttttt tatttttact tttggtgtgt tatgtcttta tacaaatggt tgctgtcagg
     3181 gtgtttcctg aatatggagt acggaaagcg gaggaacgat accgtcgaaa tccatcagca
     3241 gaaaatgagg atctgttatg gagagctaga agtcgtataa ctaaacgcaa gtaatgtttc
     3301 ctaagtgtgt tgataggcaa acatggtggg agttactact ctcggagtta atgatcttct
     3361 tgtgatcctg tcgtttcagt cgtaatctct tgctctgaaa acgaaaaaac cacctgggga
     3421 ggtggtttga tcgaaggtta agtcagttgg ggaactgctt aaccaggtaa ctgggtatac
     3481 gagaccgcag ataccaaaac tgttctttca g
//
