LOCUS       CP052542                4167 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0141 plasmid pB16KP0141-5,
            complete sequence.
ACCESSION   CP052542
VERSION     CP052542.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609869
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:16:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,124
            CDSs (total)                      :: 6,005
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 71 of 164
            Pseudo Genes (incomplete)         :: 88 of 164
            Pseudo Genes (internal stop)      :: 30 of 164
            Pseudo Genes (multiple problems)  :: 23 of 164
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0141"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0141-5"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(3090..4167,1..38))
                     /locus_tag="HJW81_30560"
     CDS             complement(join(3090..4167,1..38))
                     /locus_tag="HJW81_30560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QJM13685.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     gene            complement(383..496)
                     /locus_tag="HJW81_30565"
                     /pseudo=""
     CDS             complement(383..496)
                     /locus_tag="HJW81_30565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(521..955)
                     /locus_tag="HJW81_30570"
                     /pseudo=""
     CDS             complement(521..955)
                     /locus_tag="HJW81_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            912..1103
                     /locus_tag="HJW81_30575"
     CDS             912..1103
                     /locus_tag="HJW81_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJM13681.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1103..1504
                     /locus_tag="HJW81_30580"
     CDS             1103..1504
                     /locus_tag="HJW81_30580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJM13682.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1850..2092)
                     /locus_tag="HJW81_30585"
     CDS             complement(1850..2092)
                     /locus_tag="HJW81_30585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM13683.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2458..3090)
                     /locus_tag="HJW81_30590"
     CDS             complement(2458..3090)
                     /locus_tag="HJW81_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM13684.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="hypothetical protein"
     Typing          complement(384..791)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 aagcatttga agttctttaa acttatttcc tttatcatga tagcctctgt tcgtgttttt
       61 gttatatgcc atacgatttg ttagtacctt aataaaatgt acagctttca ttgtgattag
      121 ctagacttta tgtcaaagat taactttgat tgcttgtgtt ttaatcgatt ttttttactt
      181 tcttcttgag attatctggc agcagccgta agcttccggc tgtgaacgaa aaaaaccgcc
      241 ttggcgggcg gttttttcga aaggttcagt agagttggcg ctctatgaac cgaggtaact
      301 ggcttaaagg gagcgcagtc accaaaactg ttcgtttagt ttagctttaa ctggcgtata
      361 acttcaagac taactcccta aatcagttac cagtggctgc tgccagtggc gctttgtcgt
      421 gtctttccgg gttggactca agacgatagt taccggatta ggcgcagcag tcggactgaa
      481 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
      541 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
      601 ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct ttatagtcct
      661 gtcgggtttc gccaccactg atttgagcgt cagattccgt gatgctcgtc aggggggcgg
      721 agcctatgga aaaacggccg ctgtgcggcc tttccttctt tcctccatgc gattactcgg
      781 ttttctcgag cgtttctacg catgctcctg ccgctcgccg cagccgaacg gcaggagcgt
      841 agcgactgag tgagcgagga agcggaaaag aggagatccc tacactgacc actgacgctc
      901 tgaggcctct tatgacgaaa caacagaaaa cagcgctgaa catggctaaa ttcatccaga
      961 atcagtctct gctcctgctg gagaagctta acgagcttga tctcgatgtc gaggccgacc
     1021 tttgtgagaa actccacgat gatgcggagc atcttttccg tacactgtcc tcgagactgg
     1081 atgcattaca ggatggtaac tgatggccag agtgtctatt agtgaggctg cccgactgac
     1141 gggtaagagt agaacaacac tgcacaggtt aattaaagca ggtgacctgt ccacctgtag
     1201 cggtgagcgc aatgcaaaaa tggtagatac ttcagagctt ttacgtgtct ttggcccctt
     1261 tgagcaaccg aagggtgaac accattctgg acaggtctct ggacagcgtg atacagcctc
     1321 tgctgaccag agtgaacagg tgatccgtca tctgaaacag gagattgaac acctgaacac
     1381 cttacttttg tccaaggatt cgcacattga cagcctgaaa caggccatgc tccttcttga
     1441 aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc
     1501 ctgaccccgt catgcttcag ttttcctctc cggccatccc agggagttcc tgaaccttcc
     1561 caaaaatcgt cagatctctg aacgctcctc accactaaaa atacccacac cccacgcaaa
     1621 attaaaattt tgcagattcc ttatttacaa caatgggtta atgaaaaata aatgtgcggt
     1681 gatgtgttac aaagtgcaga tccaagtcgt ggacgactct atggcgaatc gctgacgaat
     1741 tgcccacgat tcatatgtga atcacgcttt attatcatat tgttatgatg aatcgactct
     1801 tgattcaatt catcactttg ggtgtttttt gctcatggtg actcttgatt caattcacga
     1861 ttcgaatcgc cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc
     1921 ggccgtcgac gaacccgatt cctgaaggtg ctgaaaaccg gttttccggc tgcgccggca
     1981 acgcttcacg gccacgcaat aaattgcgtc cccgttcacc gttttcggag gagaagaacg
     2041 ccggaaacag gacttcccct ggctgaatgg tgtgaatttc cgcgctatgc acttgcgcgc
     2101 atactcatgc atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt
     2161 gttgcctgtt ttaccggttt cccgtcagaa acgtcctgag tccgttttag cggtgcgtac
     2221 aattatgatt ttatgttaaa ttgttttaag tgaatagaga gaatataact taagggagca
     2281 tcttgtgctg ggtcttttac attagatctg cacaatcatt gttttggcat ctggtgatgt
     2341 ctttaggacg tttgagattg ctgataacta aagtgtatga tgctttctat acgttatccg
     2401 gcgtttaata acaccctggt gataagccag cccgtgaagg gctggcttga tttggattta
     2461 tatttttgaa ataaaatctt ttatttcatc aaagcaattg tctgctgtgt ttattattcc
     2521 atttttcatg gatattccta ttcttgagtc ggattctcta ttgcagctaa gatagacttg
     2581 taccttagct tttttttcgt ttgttggata agtgtactct ttgtcactcg ctgagatgtc
     2641 atctttatta tttttaattt tttcgaaaaa ttctattatg tattcatata acgctcgagt
     2701 gcttgcgtgt tcaattatca gatcttcaat ggcaccttgc ttgtcttttc ctggcattat
     2761 aaagaaagaa atttttgtat tgccattcat agctatctcg ttatgcgagc tgggggagga
     2821 gatttgtagt tctcgcaatg tggcaattat tgatctttct gtgtcagaaa atgatgtgtc
     2881 agcatctaat ataataaaaa tactttcttt ttcatcgaaa gaatcaatgt ttttgaatgc
     2941 tctaagtgct tgttttaatt ttgattttcc atttattttt tcgacaaaaa catcggtaat
     3001 gccttgtatt tcaagtaggt tgcatagaaa atgaaaatca tcatcaccct caacgaaaat
     3061 gatctttttg ctattattta tgtctttcat tatcttacct cccagtcgtt ctcaactgag
     3121 taggagaaat catccatatt gaattgtacg gctgttggtt ttagggagtt cttttctctt
     3181 cctagtctaa tataagcgat atcttcattt ttgctatcta tttcgtttat tgctcgtatc
     3241 acatcttgac tatgagttgt tgcaaatatt tgattgttgt tttgttttgc agatgaaatt
     3301 aatgatttta ttatgtcttt aattactgag taatgtattc cgttctcgat ttcatcaata
     3361 agaataattg agttttcctg taccagcact gagctgataa aagataatgc tcgactgata
     3421 ccctcgccaa gcattgatat ttcatttagc tcactaaatc caatgtctaa atatatttcc
     3481 ttattgttac ctatcgcaga tattgctata tctacaattc tgtcatctat tttttttaag
     3541 ttttctagta tgtctttttt ccttttttgt gttagtaggc tagatacatt tgtaattgtt
     3601 gcctctttgt ttatctttct ggatgttgtt attattgttg ctgtcttaaa tttataatta
     3661 atcccatccc tttcgttttt tatttcggat tttatttcta cgccatcttc tttcaattgc
     3721 atagagaaaa gcttattgga aaccctgttt ttcgcattgg gtgtctcggt cacatttatt
     3781 tttaaagagt ttactgtggc ctgagaaccg atagtttgac gaggtatgtt ttggttagat
     3841 agtacggtgt caacgtttag agacaaactt gattctaatc tctgatttgt ttctgtttca
     3901 tatgtttgtg ttacttcaga gcgttcatct cgaatgacta tggacatctt ttgtgatgtg
     3961 tccatatcat gaaagtaact aacccaaaat ggcttgtttt tgtttatttc tgccatttct
     4021 tttctgaatg cttgaatgtt taacaaaaca gccgggtttt ttacatcata aaaacaaaaa
     4081 atagcatcca gtatagatgt tttcccgtag ttgtttttcc cagctatgat atttaaagtc
     4141 tttaattgct ttagatatag ctcattg
//
