LOCUS       CP052541                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0141 plasmid pB16KP0141-4,
            complete sequence.
ACCESSION   CP052541
VERSION     CP052541.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609869
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:16:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,124
            CDSs (total)                      :: 6,005
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 71 of 164
            Pseudo Genes (incomplete)         :: 88 of 164
            Pseudo Genes (internal stop)      :: 30 of 164
            Pseudo Genes (multiple problems)  :: 23 of 164
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0141"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0141-4"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(7933..9294,1..603))
                     /locus_tag="HJW81_30505"
     CDS             complement(join(7933..9294,1..603))
                     /locus_tag="HJW81_30505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJM13670.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2084..2233)
                     /locus_tag="HJW81_30510"
     CDS             complement(2084..2233)
                     /locus_tag="HJW81_30510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJM13671.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2318..2575)
                     /locus_tag="HJW81_30515"
     CDS             complement(2318..2575)
                     /locus_tag="HJW81_30515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJM13672.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(2585..4270)
                     /locus_tag="HJW81_30520"
     CDS             complement(2585..4270)
                     /locus_tag="HJW81_30520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJM13673.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            4673..5092
                     /gene="exc2"
                     /locus_tag="HJW81_30525"
     CDS             4673..5092
                     /gene="exc2"
                     /locus_tag="HJW81_30525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJM13674.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            5398..5661
                     /locus_tag="HJW81_30530"
     CDS             5398..5661
                     /locus_tag="HJW81_30530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJM13675.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            5651..5950
                     /locus_tag="HJW81_30535"
     CDS             5651..5950
                     /locus_tag="HJW81_30535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJM13676.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6012..6389)
                     /locus_tag="HJW81_30540"
     CDS             complement(6012..6389)
                     /locus_tag="HJW81_30540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJM13677.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            6458..6637
                     /locus_tag="HJW81_30545"
     CDS             6458..6637
                     /locus_tag="HJW81_30545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJM13678.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6665..7195)
                     /locus_tag="HJW81_30550"
     CDS             complement(6665..7195)
                     /locus_tag="HJW81_30550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM13679.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7202..7933)
                     /locus_tag="HJW81_30555"
     CDS             complement(7202..7933)
                     /locus_tag="HJW81_30555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJM13680.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     BGC             1..9270
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1403..1981
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7206..7933)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ctggctgctg cgccggctct cctgattcag tttcgcgttc accgcctcgt cagcagccgc
       61 aaaatgaccg tcaagcagca ggaacgccag cagccgcgcc aacacaatcc cgacagccag
      121 cccgcccgcg caccaggcaa tcccgaacag gttagccggc tgcagcaggt aataatctgc
      181 gtttgcgtag aggtccggac gcaggacggt ttctccccag ctctcgataa acacggcgac
      241 aaccagcccc cccagaaggg ccagaccggc cgcgccggtc agccagtacg cgtagttcca
      301 gacatgtcga cgtgccctga ccatccgccc gggcgaatcc agcgaggcca gcaccgaaaa
      361 tggcgctggc cgtttattat cgcgcttata gcgatcggca tactcccgtc gctccttatg
      421 actcagcccc acaaaacgca tccgtccggc ccaaagttca gccagaccga aaaacaggcc
      481 gataactagg aaagccccgg ccagaccgta atactcctgg tacagccagc tgacccacgg
      541 ataaatctgc cgggccagct ccggaatgtg ccacttccag gcaagcgaat ccgtattaaa
      601 catgttctct ctccttcaga cgatggtttg ccggcgtgat gtgcagcccc cgctgcacaa
      661 gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc
      721 tttcaccacg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa
      781 caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg
      841 tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag
      901 cagctttgct gcttcgccag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt
      961 gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg acggagccag
     1021 aaagaggggg agagaccgtg cgtcggtggc gtccagccag cgtaagcagg ggatatcccc
     1081 ctgaggtcag ccacgccctc gcggggatga cctgtatttc tccgtaagga gaaaaaagca
     1141 acagagatag gttaacgtaa aatgttcagc gaaaaaccga tatgattcac ctatcctggt
     1201 aatttattat cagggaatat aaagacttaa tcaggggaca atatgccaca tcagcgcgtc
     1261 cggtgtccgt cttgtaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc
     1321 tccggccgtg agactgcggc gggcgctgcg gacacatgca aagttaccca cagattccgt
     1381 gaataagcag gggactaaca tgtgaggcaa aacggcaagg ccacgcaggt ggcgtttttc
     1441 cataggctcc gccctcctga ctgagttcac ataaacagac gctttttcgg tgcatctgtg
     1501 ggagccggtg gctcaaccat gaatcagtca ggagtggcga aacccgacag gacttaaaga
     1561 tcatcaccgt ttccgatgaa tcgctccctc ttgcgctctc ctgttccaac cctgccgttt
     1621 cccggatacc tgttccgcct ttctcccttg cgggaagtgt ggcgctttct catagctcac
     1681 actctggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaacc
     1741 ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga
     1801 aaagcacgca aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg
     1861 tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga
     1921 cagatttggt tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg
     1981 acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt
     2041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc
     2101 ccgtcagttc agaggaggac gatggtgcca ccgttccacc ctgaacatcc cggatatagt
     2161 ttgcctgaca tgcgaccaga agaaagcctg aaaaaatcaa tataaaaaga aaaacaacct
     2221 ttaccttttt catatacctt ccctgtattt aaaaaccccg gtattcatcc ctgaacaccg
     2281 gaagtctgta tttcaaatca tccttgataa cttttgctta ccagttacca tcccggtaaa
     2341 caaaggatac aaagtaatca aatttactaa tatcgattac atttttaaag tgtggctgta
     2401 atatcgaaac ccatgatttt tcaacatcaa accaaccatt attaatatta tcctttaaag
     2461 gcatccccag actttcaatg acagaaccat catcaccgaa gtcttttgag tattcaccac
     2521 ctttaaactc ttcggttttc ttatcaaacc aatgaatatg taatttaagc cccatattta
     2581 cctcttaaag atatttttta atgtttcgtt ttggatccgg ccctttaacc tgcttacccg
     2641 tttttggatc gaatgccccg aggtgttcgc catcactggc gcggtaccct tcaagctcac
     2701 cgtgctggga atcccattca taaattttac gctttttatc accataccat cgggcccgct
     2761 taccaccacc accttgttta ggggttttag gtttaccctc tttcaactct cccaacccct
     2821 taatatcttc agtcttggga tcaggaaaat aatcatggcc gtaatcttta gttcccttac
     2881 gaggtttttt cttttcctca tttaatttat tttctgcatc cttcgctttt ttctcctttt
     2941 gtttccggct ctccatcgcc ccggaaagcg cagccttatc cctggataag gattcattca
     3001 tttcgtttaa agatgtctgg gcagctttta atttctcatt ctgattctga atatccttat
     3061 caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca
     3121 caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag
     3181 cccttgcacc ggggatcgcc ttctctgtcg atgcaatgcg actgtttaag gtcgcaatat
     3241 ttttatcttc agcatccagc tcagctttcg ctttatcata ctctcttttc agcccctctt
     3301 ccgggtgagc agcgtcccat gcctgctggc gacgcttttc ttcctccaga cgctgtttta
     3361 cctgtgccgg agtaagaaca tccgtcaccg acacatagac cggtttctgt ccgctctctt
     3421 tcgggaaacg aataacggcc tcacgggagt taccaccggc cgtaaaacca gccggacgtg
     3481 aatcaccttt ttcagcaata atgccttttg gcggggcttt cgcggccgga acacctttag
     3541 gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt
     3601 ttgtcggttt cgcatccacc acaggaacac tcattggccg gccggtgaca accgcaatat
     3661 gctgccgctc gtccttcaca atatccgcca cacgtttagt gacgctgacc gtcgcctggt
     3721 ccagcggcag ggagctgacc ggcgtctccg ttaccgtgtc tgccggcaat gacgtcacga
     3781 tttttgacat cattttcgga tcatcttttg ctatctcaga aggcagcact ccgtaaatcg
     3841 caatccccca cagaccaaac ttaaacggac ctttcagggc agccagcaca tcagcaacag
     3901 cggccgacaa cgcatcgccg gaaacggata aagccagtcc ttcagccccc ggagtggcca
     3961 gggcaggcag accgaaggcc atcgcggtgg ccgaagactg tccgccacca gagctcccgc
     4021 tatgattgcc gctgttaccg ccattaccac cgggtttact cccgccgtta ccaaactccc
     4081 ccgggttata gttatgaata tccccgttag gtgtatgcgt cgtaccccag cccggcccgc
     4141 tgtttggatc cgtaccacca cctgatgaag ggccaccttt acctgatgta ccgttcacat
     4201 tcccactggc ctgaccacca ttgtgtccca gacctgaatt acccggacct cgtccgtctc
     4261 cgccactcat atacttttcc tctttgactt ttaaaacaat aaactaaaat aaaatactgt
     4321 acatataacc actggtttta tatacagtat aaaaattacg ctgccttgtt tttcatgtca
     4381 agtctgtaaa tttgcgtttt tgtgaaaatg atcaaaaaaa cagccactca caccatgcgc
     4441 aatcccctcc agatgcaaca aaaaccagca caaacggcgc cgttctccgg cctcagtatg
     4501 ttttactgaa atcattatat gagataccag aaagccccct ccgggacagg aaaagaaacg
     4561 gggggtctgg ttcgggttgt tcggtcactg aacacggtca gaaactgatg ataagatagc
     4621 ctgcggatat ataactcaat gagaaaaaag aattaaaggt aaggagacgc tcatgagacg
     4681 ttccggcagc aaactgtgtc ttatcgccat gataacagcg acagcaactc tcactgcctg
     4741 tgctccaaag ggcagcgtgg aacagcatac ccggcattac gtatatgctt cagatgacgg
     4801 ttttgatccc aatttttcca cccagaaagc tgacacaaca cgaatgatgg tgcctttttt
     4861 ccggcagttc tgggatatgg gcgcaaagga taaagcgacc ggaaaatcac ggagtgatgt
     4921 gcagcaacgc attcagcagt ttcacagtca ggaattttta aactcactcc aaggcacaac
     4981 acaatttgcg ggtaccgatt accgcagcaa agacctcaca ccgaaaaaat caaggctact
     5041 ggctgacacc atttcagctg tttatctcga tggctatgaa ggcagacagt aagtggattt
     5101 accataacgc gtcattacgc gcaccgctca gacgggctca gggcgtttcc ggcggctacg
     5161 ggataaaaca ggcaacaacc cccccggaaa actgccggaa acgcgcccga taaattttat
     5221 cctaatgcat gcctatgcac gcaactgcat ggcatatgtt ttgacgtaaa atggatgccg
     5281 taagcccttt tttaccgact ttctgcttca tcagcctgac tgacacgacc atattctttc
     5341 agaccgcccc ctgctaaact tccttcatat tgccgcatta acccgggagg ctccgcaatg
     5401 agcaacacta cacactacga aaacgccaac tttcttcggg aactggctga aagcctgccc
     5461 cgcatactgc cggaaggtgg cccggacaaa gctgcgctgt tgcagcgact ggctaacgaa
     5521 gaactggccc aggcagagta tgaagatcag gttcgcgcca aagtgaccgc cgcccgtgct
     5581 gacacgcgtc cgggtatgac cacggaacaa ctccgccagc gcctgcatgg ccgttatcag
     5641 gaactgcgcg atgccgtatg acgtggaatg gaagcaggga gccatcgagg atgtaacgac
     5701 gctgtttgac tatatcgcgg aaaattcatc cctgtgggat gcgcagaacg ttaccgaccg
     5761 ggttctcgct gcggcggaca aactggccga cttcccgcga ctctatgaag tggactcacg
     5821 ctatggcgag gacgttcgcc ggatcagtct gatgggacag catgtcctct atgatgttga
     5881 tgaccagacc agaaaagtcc gggttctggc cgtggtcggc cagcgccaga atccccatat
     5941 tgttctctaa taaccggtga cggggacgca attcattgcg tggccgtcgc cggttggaca
     6001 agcacagcgc gtcagcgttt tccccacggc caccaccggc gcggtgttcc ggccggggtc
     6061 tctgctgtct ccggtttgtg ttccagcagt ctcatggact gcatttcctg ccgtagcccg
     6121 gccacctcct ccctgagtgc ttttatctcc gcgatcaacg cgtcggaggc cattttttca
     6181 gtctcacccg acagtctcaa ctcgtcccgc tcgtgagtct catcctgtct taactcaccg
     6241 tacacccgga taagctcgct ggtatccacc acccggccgc catcagcttc cgtacggtat
     6301 gatacgcggc ctttcgccat atcccgatac agggaacttc gggctttacc cgtcagttcc
     6361 gtcgcctgtc tcaccgtcac ccacgccatt gtctcacccc gctatttttg agacagtata
     6421 cctgacaggc gcacaccatg cccggaagga aagaatcatg acaaaacagg aaaaaacagc
     6481 cctcaatatg gctaaattta ttaaggagca gtcgctgcta ttgctcgata aactgaatga
     6541 gctggatatg gataccccgg ccgacatgtg cgagcggcta cacgaggatg cggaaacact
     6601 ctggcaggtg atgcgggata agctcgggga ggaataacag caccggcatg gccggtgctg
     6661 gcatttattt tttgccgaac ggccaccacc ggcgggcagg tttgtcctcc ggcagttcag
     6721 gtaccggcac gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct
     6781 cgttcaccgc gctttccccg tgccgctcac tcaccacgtc aatcatcgcg ttaatctgct
     6841 ccggtgagca cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca
     6901 ccttgcgata gaggtaatca cacagcttca ttcctgtggg tttgttccgt tcccattgcg
     6961 gatttttacc agtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca
     7021 tcccgatgga tttcatcatc gccagatgct gggcccggtc gacaaaacag aaaacatacg
     7081 ccccggtctg ccaggcgagc accaccatat cttccagatg ctcgctggtc ggcatgatgc
     7141 agcgctcgcc gttccggtcc tccgcgtctt caataaatac catgtacagc gccataatcc
     7201 ctcactccgt tgtcataaat ttaaagcacg caaacaaccg ttcacgatcc agctctgccg
     7261 ttttgcgggc ctcgccggtc tgtgggttca cgatttcatc caccgacagg attgcccgct
     7321 gcaccctgac ctgcgtcgcc cggtccgggc agatgtacca gatttcatcg atgccgttag
     7381 ctttgcggtt gaacagatac ccggacacca cttcctgata ccggcgacgg ctcttgacgg
     7441 tgcgctccac ctcaaacgcc accgttttac cgtccagctc cgccagggcg tccggcactt
     7501 tcatgccttt aagactcaga cggtgcagaa tgcgccagcc ggtggcccct ctggcttcca
     7561 tatcaaggcg cagggattgc acggccagcc ggtgcggcag atgtgctgcc gacacccttg
     7621 acggctgaaa cggaataaca tccgccagcg gttcgtcctc gtcgaacgag aacgccacgc
     7681 catgcggcgt cagcccccac acgctgacct gacggccgtc caccggatat ttggtccgga
     7741 ccagcagccc tttcgccacc aggctgtcga ggaagcggta gaccggctgc accgccgcaa
     7801 tccccagccg ccgcgcaacc agctccgccg tggaacacag ctcttcccgc aaccagcgca
     7861 gggtcagccg ggttttctcc gcgttccgcg cctgtcgttt cgcgtggctg acgttgtaca
     7921 gctccagcgc catcagtaaa tccccgcaaa atcatcatcc gctttcggcc cgccgccgtc
     7981 ggccgggggc cggaactccg ccaggggatc ggttttcgcc agcaccggga aacgctgcaa
     8041 cgggatttcc cggcgctcaa cccgcagcgg actgctgaac gccatccggg ccacacccag
     8101 cccggtcatg accgcgaacc cgtccggcag gtgttgcagg gtgttcacgt caaacagcgg
     8161 ccgcgcctcc ttctggatga agctgtcccc gctgatatgc tccacgttcc ccgcctcccg
     8221 gctggcacgg cggcgctcca cgtccaccct gatttccccg gtctgccccg ccgcccactg
     8281 cgcggactcc aggctggccg cccggtaaaa ccagcggatc ggcgtgttat ccagtaccgt
     8341 ggttttcaca aattcagcgg gcagatcctg cccgcactgc ccgaaatcgc ccagcgactg
     8401 gtgcgcgagc aacagattgc agtcccggct gcgcagcgtc cccagtgcgt tcagtacata
     8461 cttcgacagc agatatttaa tttcatcgag catcacgctg acgtgtggcc actcccggaa
     8521 ctcgtcccgc gcaatgatga tttgcgcgca gcgggcaaac agcatttttt gcacacggat
     8581 caccgcctcg tcgtccatcg agccgataac gtacaggcag ccgccgccgt tgataatccc
     8641 cgccacatcg atgccactgt ccgtctgcag gacgctcagg tcagcgacgt tttccagcct
     8701 agtgatcaac cccttcacgt cctttttcag ggcctccggc agcgcgtacg ccgccgccag
     8761 cacctgctga atgtttgccc cctgcggaaa ctgctccgca atcagcttcg ccgctttctg
     8821 ctcgctgatg cggtagtggt ccgccacgtc gccggtctcg cccaggttaa gcccggcaat
     8881 cagcaggttt ttcagcgcgt actggtcgag atcgcggaac agattcagct gcgccggttt
     8941 ccccggccgc agatccagca gggtgaagtc cggacagtgc gcccggaaga cgtgcggcgc
     9001 ccatgcgtcc cctttggggt cgaagtacac caccgcgtcc ccgaaatcgc gtacgcagcg
     9061 aatgagcgcg ttggtggcca tcacgctctt gccgctcccg ggcaggccga ggatctgaat
     9121 gttggtcttg cgccaggtat cacgcggaac cagcaccggc ttgccctgct catccttacc
     9181 cagaaacacc gcctgctcct gccgggcctg ctccgaggaa aaatcggcca gcgcattaac
     9241 cggtaccggc tccccgaaat gcaggtggcg cacgtcggtc atctctcctg tccg
//
