LOCUS       CP052536                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0157 plasmid pB16KP0157-3,
            complete sequence.
ACCESSION   CP052536
VERSION     CP052536.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609870
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:18:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,887
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,609
            CDSs (with protein)               :: 5,609
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 66 of 157
            Pseudo Genes (incomplete)         :: 90 of 157
            Pseudo Genes (internal stop)      :: 23 of 157
            Pseudo Genes (multiple problems)  :: 19 of 157
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0157"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0157-3"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(9019..9294,1..144))
                     /gene="exc2"
                     /locus_tag="HJW82_29385"
     CDS             complement(join(9019..9294,1..144))
                     /gene="exc2"
                     /locus_tag="HJW82_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJM02564.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            547..2232
                     /locus_tag="HJW82_29390"
     CDS             547..2232
                     /locus_tag="HJW82_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJM02565.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            2242..2499
                     /locus_tag="HJW82_29395"
     CDS             2242..2499
                     /locus_tag="HJW82_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJM02566.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            2584..2733
                     /locus_tag="HJW82_29400"
     CDS             2584..2733
                     /locus_tag="HJW82_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJM02567.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4214..6178
                     /locus_tag="HJW82_29405"
     CDS             4214..6178
                     /locus_tag="HJW82_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJM02568.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            6178..6909
                     /locus_tag="HJW82_29410"
     CDS             6178..6909
                     /locus_tag="HJW82_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJM02569.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            6916..7446
                     /locus_tag="HJW82_29415"
     CDS             6916..7446
                     /locus_tag="HJW82_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM02570.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7474..7653)
                     /locus_tag="HJW82_29420"
     CDS             complement(7474..7653)
                     /locus_tag="HJW82_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJM02571.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7722..8099
                     /locus_tag="HJW82_29425"
     CDS             7722..8099
                     /locus_tag="HJW82_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJM02572.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(8161..8460)
                     /locus_tag="HJW82_29430"
     CDS             complement(8161..8460)
                     /locus_tag="HJW82_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJM02573.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8450..8713)
                     /locus_tag="HJW82_29435"
     CDS             complement(8450..8713)
                     /locus_tag="HJW82_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJM02574.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     BGC             1..7232
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2837..3415)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6179..6906
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 aaaattggga tcaaaaccgt catctgaagc atatacgtaa tgccgggtat gctgttccac
       61 gctgcccttt ggagcacagg cagtgagagt tgctgtcgct gttatcatgg cgataagaca
      121 cagtttgctg ccggaacgtc tcatgagcgt ctccttacct ttaattcttt tttctcattg
      181 agttatatat ccgcaggcta tcttatcatc agtttctgac cgtgttcagt gaccgaacaa
      241 cccgaaccag accccccgtt tcttttcctg tcccggaggg ggctttctgg tatctcatat
      301 aatgatttca gtaaaacata ctgaggccgg agaacggcgc cgtttgtgct ggtttttgtt
      361 gcatctggag gggattgcgc atggtgtgag tggctgtttt tttgatcatt ttcacaaaaa
      421 cgcaaattta cagacttgac atgaaaaaca aggcagcgta atttttatac tgtatataaa
      481 accagtggtt atatgtacag tattttattt tagtttattg ttttaaaagt caaagaggaa
      541 aagtatatga gtggcggaga cggacgaggt ccgggtaatt caggtctggg acacaatggt
      601 ggtcaggcca gtgggaatgt gaacggtaca tcaggtaaag gtggcccttc atcaggtggt
      661 ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacacctaa cggggatatt
      721 cataactata acccggggga gtttggtaac ggcgggagta aacccggtgg taatggcggt
      781 aacagcggca atcatagcgg gagctctggt ggcggacagt cttcggccac cgcgatggcc
      841 ttcggtctgc ctgccctggc cactccgggg gctgaaggac tggctttatc cgtttccggc
      901 gatgcgttgt cggccgctgt tgctgatgtg ctggctgccc tgaaaggtcc gtttaagttt
      961 ggtctgtggg ggattgcgat ttacggagtg ctgccttctg agatagcaaa agatgatccg
     1021 aaaatgatgt caaaaatcgt gacgtcattg ccggcagaca cggtaacgga gacgccggtc
     1081 agctccctgc cgctggacca ggcgacggtc agcgtcacta aacgtgtggc ggatattgtg
     1141 aaggacgagc ggcagcatat tgcggttgtc accggccggc caatgagtgt tcctgtggtg
     1201 gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct
     1261 ctgcaggtga gcgtacctaa aggtgttccg gccgcgaaag ccccgccaaa aggcattatt
     1321 gctgaaaaag gtgattcacg tccggctggt tttacggccg gtggtaactc ccgtgaggcc
     1381 gttattcgtt tcccgaaaga gagcggacag aaaccggtct atgtgtcggt gacggatgtt
     1441 cttactccgg cacaggtaaa acagcgtctg gaggaagaaa agcgtcgcca gcaggcatgg
     1501 gacgctgctc acccggaaga ggggctgaaa agagagtatg ataaagcgaa agctgagctg
     1561 gatgctgaag ataaaaatat tgcgacctta aacagtcgca ttgcatcgac agagaaggcg
     1621 atccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca
     1681 aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat
     1741 caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa agctgcccag
     1801 acatctttaa acgaaatgaa tgaatcctta tccagggata aggctgcgct ttccggggcg
     1861 atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa attaaatgag
     1921 gaaaagaaaa aacctcgtaa gggaactaaa gattacggcc atgattattt tcctgatccc
     1981 aagactgaag atattaaggg gttgggagag ttgaaagagg gtaaacctaa aacccctaaa
     2041 caaggtggtg gtggtaagcg ggcccgatgg tatggtgata aaaagcgtaa aatttatgaa
     2101 tgggattccc agcacggtga gcttgaaggg taccgcgcca gtgatggcga acacctcggg
     2161 gcattcgatc caaaaacggg taagcaggtt aaagggccgg atccaaaacg aaacattaaa
     2221 aaatatcttt aagaggtaaa tatggggctt aaattacata ttcattggtt tgataagaaa
     2281 accgaagagt ttaaaggtgg tgaatactca aaagacttcg gtgatgatgg ttctgtcatt
     2341 gaaagtctgg ggatgccttt aaaggataat attaataatg gttggtttga tgttgaaaaa
     2401 tcatgggttt cgatattaca gccacacttt aaaaatgtaa tcgatattag taaatttgat
     2461 tactttgtat cctttgttta ccgggatggt aactggtaag caaaagttat caaggatgat
     2521 ttgaaataca gacttccggt gttcagggat gaataccggg gtttttaaat acagggaagg
     2581 tatatgaaaa aggtaaaggt tgtttttctt tttatattga ttttttcagg ctttcttctg
     2641 gtcgcatgtc aggcaaacta tatccgggat gttcagggtg gaacggtggc accatcgtcc
     2701 tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc
     2761 ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt
     2821 tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc
     2881 acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac
     2941 cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg
     3001 gcgttttgcg tgcttttccg ggttggactc aagtgaacag ttaccggata aggcgcagca
     3061 gtcgggctga acggggggtt cttgcttaca gcccagcttg gagcgaacga cctacaccga
     3121 gccgagatac cagagtgtga gctatgagaa agcgccacac ttcccgcaag ggagaaaggc
     3181 ggaacaggta tccgggaaac ggcagggttg gaacaggaga gcgcaagagg gagcgattca
     3241 tcggaaacgg tgatgatctt taagtcctgt cgggtttcgc cactcctgac tgattcatgg
     3301 ttgagccacc ggctcccaca gatgcaccga aaaagcgtct gtttatgtga actcagtcag
     3361 gagggcggag cctatggaaa aacgccacct gcgtggcctt gccgttttgc ctcacatgtt
     3421 agtcccctgc ttattcacgg aatctgtggg taactttgca tgtgtccgca gcgcccgccg
     3481 cagtctcacg gccggagcgt agcgaccgag tgagcgagga ggcgcaattt ccccggcacc
     3541 tacaagacgg acaccggacg cgctgatgtg gcatattgtc ccctgattaa gtctttatat
     3601 tccctgataa taaattacca ggataggtga atcatatcgg tttttcgctg aacattttac
     3661 gttaacctat ctctgttgct tttttctcct tacggagaaa tacaggtcat ccccgcgagg
     3721 gcgtggctga cctcaggggg atatcccctg cttacgctgg ctggacgcca ccgacgcacg
     3781 gtctctcccc ctctttctgg ctccgtccgg ctggacgccg gccacgccag aaattcaccc
     3841 ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg acgcacctgg
     3901 cgaagcagca aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct
     3961 gacgggggcc ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc
     4021 tggggcgtgc tcgttgttcc tccggggctg cgccttttcc gcatggggcg gaaagaggct
     4081 ggcctccgtg gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc gccatgcctt
     4141 ggggcatgtc cgcggcttgt gcagcggggg ctgcacatca cgccggcaaa ccatcgtctg
     4201 aaggagagag aacatgttta atacggattc gcttgcctgg aagtggcaca ttccggagct
     4261 ggcccggcag atttatccgt gggtcagctg gctgtaccag gagtattacg gtctggccgg
     4321 ggctttccta gttatcggcc tgtttttcgg tctggctgaa ctttgggccg gacggatgcg
     4381 ttttgtgggg ctgagtcata aggagcgacg ggagtatgcc gatcgctata agcgcgataa
     4441 taaacggcca gcgccatttt cggtgctggc ctcgctggat tcgcccgggc ggatggtcag
     4501 ggcacgtcga catgtctgga actacgcgta ctggctgacc ggcgcggccg gtctggccct
     4561 tctggggggg ctggttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgtcc
     4621 ggacctctac gcaaacgcag attattacct gctgcagccg gctaacctgt tcgggattgc
     4681 ctggtgcgcg ggcgggctgg ctgtcgggat tgtgttggcg cggctgctgg cgttcctgct
     4741 gcttgacggt cattttgcgg ctgctgacga ggcggtgaac gcgaaactga atcaggagag
     4801 ccggcgcagc agccagcgga caggagagat gaccgacgtg cgccacctgc atttcgggga
     4861 gccggtaccg gttaatgcgc tggccgattt ttcctcggag caggcccggc aggagcaggc
     4921 ggtgtttctg ggtaaggatg agcagggcaa gccggtgctg gttccgcgtg atacctggcg
     4981 caagaccaac attcagatcc tcggcctgcc cgggagcggc aagagcgtga tggccaccaa
     5041 cgcgctcatt cgctgcgtac gcgatttcgg ggacgcggtg gtgtacttcg accccaaagg
     5101 ggacgcatgg gcgccgcacg tcttccgggc gcactgtccg gacttcaccc tgctggatct
     5161 gcggccgggg aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgcgctgaa
     5221 aaacctgctg attgccgggc ttaacctggg cgagaccggc gacgtggcgg accactaccg
     5281 catcagcgag cagaaagcgg cgaagctgat tgcggagcag tttccgcagg gggcaaacat
     5341 tcagcaggtg ctggcggcgg cgtacgcgct gccggaggcc ctgaaaaagg acgtgaaggg
     5401 gttgatcact aggctggaaa acgtcgctga cctgagcgtc ctgcagacgg acagtggcat
     5461 cgatgtggcg gggattatca acggcggcgg ctgcctgtac gttatcggct cgatggacga
     5521 cgaggcggtg atccgtgtgc aaaaaatgct gtttgcccgc tgcgcgcaaa tcatcattgc
     5581 gcgggacgag ttccgggagt ggccacacgt cagcgtgatg ctcgatgaaa ttaaatatct
     5641 gctgtcgaag tatgtactga acgcactggg gacgctgcgc agccgggact gcaatctgtt
     5701 gctcgcgcac cagtcgctgg gcgatttcgg gcagtgcggg caggatctgc ccgctgaatt
     5761 tgtgaaaacc acggtactgg ataacacgcc gatccgctgg ttttaccggg cggccagcct
     5821 ggagtccgcg cagtgggcgg cggggcagac cggggaaatc agggtggacg tggagcgccg
     5881 ccgtgccagc cgggaggcgg ggaacgtgga gcatatcagc ggggacagct tcatccagaa
     5941 ggaggcgcgg ccgctgtttg acgtgaacac cctgcaacac ctgccggacg ggttcgcggt
     6001 catgaccggg ctgggtgtgg cccggatggc gttcagcagt ccgctgcggg ttgagcgccg
     6061 ggaaatcccg ttgcagcgtt tcccggtgct ggcgaaaacc gatcccctgg cggagttccg
     6121 gcccccggcc gacggcggcg ggccgaaagc ggatgatgat tttgcgggga tttactgatg
     6181 gcgctggagc tgtacaacgt cagccacgcg aaacgacagg cgcggaacgc ggagaaaacc
     6241 cggctgaccc tgcgctggtt gcgggaagag ctgtgttcca cggcggagct ggttgcgcgg
     6301 cggctgggga ttgcggcggt gcagccggtc taccgcttcc tcgacagcct ggtggcgaaa
     6361 gggctgctgg tccggaccaa atatccggtg gacggccgtc aggtcagcgt gtgggggctg
     6421 acgccgcatg gcgtggcgtt ctcgttcgac gaggacgaac cgctggcgga tgttattccg
     6481 tttcagccgt caagggtgtc ggcagcacat ctgccgcacc ggctggccgt gcaatccctg
     6541 cgccttgata tggaagccag aggggccacc ggctggcgca ttctgcaccg tctgagtctt
     6601 aaaggcatga aagtgccgga cgccctggcg gagctggacg gtaaaacggt ggcgtttgag
     6661 gtggagcgca ccgtcaagag ccgtcgccgg tatcaggaag tggtgtccgg gtatctgttc
     6721 aaccgcaaag ctaacggcat cgatgaaatc tggtacatct gcccggaccg ggcgacgcag
     6781 gtcagggtgc agcgggcaat cctgtcggtg gatgaaatcg tgaacccaca gaccggcgag
     6841 gcccgcaaaa cggcagagct ggatcgtgaa cggttgtttg cgtgctttaa atttatgaca
     6901 acggagtgag ggattatggc gctgtacatg gtatttattg aagacgcgga ggaccggaac
     6961 ggcgagcgct gcatcatgcc gaccagcgag catctggaag atatggtggt gctcgcctgg
     7021 cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa
     7081 tccatcggga tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcact
     7141 ggtaaaaatc cgcaatggga acggaacaaa cccacaggaa tgaagctgtg tgattacctc
     7201 tatcgcaagg tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag
     7261 tgctcaccgg agcagattaa cgcgatgatt gacgtggtga gtgagcggca cggggaaagc
     7321 gcggtgaacg agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac
     7381 gtgccggtac ctgaactgcc ggaggacaaa cctgcccgcc ggtggtggcc gttcggcaaa
     7441 aaataaatgc cagcaccggc catgccggtg ctgttattcc tccccgagct tatcccgcat
     7501 cacctgccag agtgtttccg catcctcgtg tagccgctcg cacatgtcgg ccggggtatc
     7561 catatccagc tcattcagtt tatcgagcaa tagcagcgac tgctccttaa taaatttagc
     7621 catattgagg gctgtttttt cctgttttgt catgattctt tccttccggg catggtgtgc
     7681 gcctgtcagg tatactgtct caaaaatagc ggggtgagac aatggcgtgg gtgacggtga
     7741 gacaggcgac ggaactgacg ggtaaagccc gaagttccct gtatcgggat atggcgaaag
     7801 gccgcgtatc ataccgtacg gaagctgatg gcggccgggt ggtggatacc agcgagctta
     7861 tccgggtgta cggtgagtta agacaggatg agactcacga gcgggacgag ttgagactgt
     7921 cgggtgagac tgaaaaaatg gcctccgacg cgttgatcgc ggagataaaa gcactcaggg
     7981 aggaggtggc cgggctacgg caggaaatgc agtccatgag actgctggaa cacaaaccgg
     8041 agacagcaga gaccccggcc ggaacaccgc gccggtggtg gccgtgggga aaacgctgac
     8101 gcgctgtgct tgtccaaccg gcgacggcca cgcaatgaat tgcgtccccg tcaccggtta
     8161 ttagagaaca atatggggat tctggcgctg gccgaccacg gccagaaccc ggacttttct
     8221 ggtctggtca tcaacatcat agaggacatg ctgtcccatc agactgatcc ggcgaacgtc
     8281 ctcgccatag cgtgagtcca cttcatagag tcgcgggaag tcggccagtt tgtccgccgc
     8341 agcgagaacc cggtcggtaa cgttctgcgc atcccacagg gatgaatttt ccgcgatata
     8401 gtcaaacagc gtcgttacat cctcgatggc tccctgcttc cattccacgt catacggcat
     8461 cgcgcagttc ctgataacgg ccatgcaggc gctggcggag ttgttccgtg gtcatacccg
     8521 gacgcgtgtc agcacgggcg gcggtcactt tggcgcgaac ctgatcttca tactctgcct
     8581 gggccagttc ttcgttagcc agtcgctgca acagcgcagc tttgtccggg ccaccttccg
     8641 gcagtatgcg gggcaggctt tcagccagtt cccgaagaaa gttggcgttt tcgtagtgtg
     8701 tagtgttgct cattgcggag cctcccgggt taatgcggca atatgaagga agtttagcag
     8761 ggggcggtct gaaagaatat ggtcgtgtca gtcaggctga tgaagcagaa agtcggtaaa
     8821 aaagggctta cggcatccat tttacgtcaa aacatatgcc atgcagttgc gtgcataggc
     8881 atgcattagg ataaaattta tcgggcgcgt ttccggcagt tttccggggg ggttgttgcc
     8941 tgttttatcc cgtagccgcc ggaaacgccc tgagcccgtc tgagcggtgc gcgtaatgac
     9001 gcgttatggt aaatccactt actgtctgcc ttcatagcca tcgagataaa cagctgaaat
     9061 ggtgtcagcc agtagccttg attttttcgg tgtgaggtct ttgctgcggt aatcggtacc
     9121 cgcaaattgt gttgtgcctt ggagtgagtt taaaaattcc tgactgtgaa actgctgaat
     9181 gcgttgctgc acatcactcc gtgattttcc ggtcgcttta tcctttgcgc ccatatccca
     9241 gaactgccgg aaaaaaggca ccatcattcg tgttgtgtca gctttctggg tgga
//
