LOCUS       CP052532                3511 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0177 plasmid pB16KP0177-8,
            complete sequence.
ACCESSION   CP052532
VERSION     CP052532.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609871
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,715
            Genes (coding)                    :: 5,604
            CDSs (with protein)               :: 5,604
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 51 of 111
            Pseudo Genes (incomplete)         :: 61 of 111
            Pseudo Genes (internal stop)      :: 18 of 111
            Pseudo Genes (multiple problems)  :: 18 of 111
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0177"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0177-8"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(3278..3511,1..108)
                     /locus_tag="HJW83_29160"
     CDS             join(3278..3511,1..108)
                     /locus_tag="HJW83_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJO48821.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            join(3507..3511,1..805)
                     /locus_tag="HJW83_29165"
                     /pseudo=""
     CDS             join(3507..3511,1..805)
                     /locus_tag="HJW83_29165"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="internal stop; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            786..1283
                     /locus_tag="HJW83_29170"
     CDS             786..1283
                     /locus_tag="HJW83_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJO48822.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            1290..1505
                     /gene="mbeD"
                     /locus_tag="HJW83_29175"
     CDS             1290..1505
                     /gene="mbeD"
                     /locus_tag="HJW83_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJO48823.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mbeD"
     gene            1900..2082
                     /locus_tag="HJW83_29180"
     CDS             1900..2082
                     /locus_tag="HJW83_29180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO48824.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(2682..2993)
                     /locus_tag="HJW83_29185"
     CDS             complement(2682..2993)
                     /locus_tag="HJW83_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO48825.1"
                     /translation="MRRAEMAYERGGDFLEDARKILNREAIGPRRSRFSIGSAPLTSIT
                     KSDAQISGGETRQDYKDTRRFPLAAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     Typing          3020..3193
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2532..3053)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          99..1621
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3300..3511
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.366"
                     /coverage="98.0"
                     /evalue="5.17e-39"
ORIGIN
        1 gccggtcatg accgggtgca gatcgtggca gcgctgatgg cgatcgatgc cggtctggaa
       61 cggttgcggc atgccgtact ggaaaaaggg ccggacgatg attgttaaat tccatccccg
      121 gggtcgcggc gggggcgccg gtcctgtgga ttatcttctg ggtaaagacc ggcagcgtga
      181 aggtgccagc gtcctgcagg ggaagccgga agaagtccgg gagctgattg atgcgtcgcc
      241 gtatgtgaaa aaatatacct ccggggtgct gtcattcgcg gaggcggacc tgccgccggg
      301 tcagcgtgaa aaactgatgg ccagctttga gcgggttctg atgcccggac tcgataaaga
      361 ccagtacagc attctgtggg tggaacacgc agacaagggt cgtcttgagc tgaattttct
      421 gatccccaat accgaattac tgacggggaa acgcctccag ccgtattacg atcgtgccga
      481 ccggccgcgc atcgatgcgt ggcagacagt ggtcaacggc aggctggggc tacatgatcc
      541 gaacgacccg gagaaccggc gggcgctggt cacgccttca ggactgccag aaacgaagca
      601 ggaagccgca gagacgatta cacggggttt gctgatgtta gcctcatcag gagagctgaa
      661 aacgcgtgat gacgtcacag gggcgctcac agcggccggt tttgaggtgg tcagaaccac
      721 aaaaaacagt atcagcattg ccgacccgga cggggggcga aatatccgac tgaggggagc
      781 catttatgag cagtctttta acggcctcga gggacttcga ggagaaatcg aaagcacagc
      841 agcagcttac cgaagagagg ctgaaagccg cattcagcaa gcacgagaac gttgtcaaat
      901 cggaactgaa cgcaagcgcg aagagaatca acgacgccat cagcgcccac gaacagggga
      961 tgaacgcggc gatgcagtcg aaccggctga gcgtgctgcg aatggtcggg cggacgtggc
     1021 tgaccatcac catggtgtca gggctgctgt tcgccagcct gagcggggtg ctgtggtacc
     1081 aggggaaccg gatagcggcg aacctggcgg aaatcaggca gcagagagat acgctgtcga
     1141 aactgcacat gcagacgtgg ggcgtgacct atctggagaa ccgcaacgga cggtttctgg
     1201 tgctgccgga ggggatgaaa gccgaaacgg gccggacggt ggacaacaag acgcggaacg
     1261 cggtgaagct ggtacgggag tagagcagaa tgacggaact ggaaaaacaa ttgctgagcg
     1321 cgttagagca gctacagcag gactactcga gaaggctgga cgagtgggag agcgccttcg
     1381 aggaatggcg gaagatgtct ggtcttatgc aacgggagaa cgcggcgctg agcgagcgcg
     1441 tgtcgggctt gagcaggcag gtgcagagtt tgagcgagca gctgcgccgg ttatcgacag
     1501 gctgaatgag attgagcaac agcgatacgg gcaaacaacg gaaaaggaac cagagaagac
     1561 agcagatctg gtaccggaac ggcaacgcac attatctcat ggtgacaggg agtatggaga
     1621 ctgataaacg cccgtctcag gtggcctgag cgtgacgggg atcatggcca ttttctgcga
     1681 tttcagcaca cagagagtct tttttaaccg aaatcaccgg ctatgcagtg atgtgaataa
     1741 gtgatgcata tgcgtaaaat aaatcaggtg cgtttctggt ggttttcggt gtacttgcca
     1801 ccaccttttg cccgtcagcc atcagaaacg ccctgagtgc ctccggcgcg gtgcgggtaa
     1861 gagatgttat gtttaatgtt gataacagga gtaagagtga tgcttacatt tattggtgta
     1921 gtggtagtcg ccgttattgc atttaaatta tttatgcttt tttttagttc atccgaatct
     1981 gccgtcgaac gtgctacccg taaatatatg gagaacccta cgagtgctaa ctataaaata
     2041 atggtggcta cacgcatgag aaatgagaag aaaagagggt gatatatgtt tgagtttatt
     2101 ggttttttat ttttactttt ggtgtgttat gtctttatac aaatggttgc tgtcagggtg
     2161 tttcctgaat atggagtacg gaaagcggag gaacgatacc gtcgaaatcc atcagcagaa
     2221 aatgaggatc tgttatggag agctagaagt cgtataacta aacgcaagta atgtttccta
     2281 agtgtgttga taggcaaaca tggtgggagt tactactctc ggagttaatg atcttcttgt
     2341 gatcctgtcg tttcagtcgt aatctcttgc tctgaaaacg aaaaaaccac ctggggaggt
     2401 ggtttgatcg aaggttaagt cagttgggga actgcttaac caggtaactg ggtatacgag
     2461 accgcagata ccaaaactgt tctttcagtg tagccgcagt caggcccata cttcaagacc
     2521 tctgaatctc tctctcgtat atcccagtta ccagtggctg ctgccagtgg cgctttgtcg
     2581 tgctttctcg ggttggactc aagatgatag ttaccgggta aggcgcagca gtcggactga
     2641 acggggggtt tgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc
     2701 aggcgtggaa tgagataaac gcggccataa cagcggaatg acaccggtaa accgaaaggc
     2761 aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc
     2821 tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg
     2881 gagcctatgg aaaaacggct tcgccgcggc cctatcgctt ccctgttaag tatcttcctg
     2941 gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg
     3001 accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc
     3061 tgacgcgccg gtgccgcctt ttttctcctg ccacatgaag cacttcactg acatcctcat
     3121 ccgtgccaac atagtaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
     3181 gcgccccgaa accccactga cgcgcctgcg gcttgtccaa ccccgcgccg accgggaaag
     3241 gctttcccgt ccgtcacggc gttatcagga ggcgattctg gcagataaac gcaacaagat
     3301 gctcacgatg tgggtgacgg aagacgaaca ccggcggttg cttgagcggt gcgacggaaa
     3361 acaactggcg gcgtggatgc gccagacctg ccttgatgag aaaccgacac gcgccggcag
     3421 gcttccctcg atttcgcccg cgctgctgcg tcagcttgcc ggcatgggca ataacctgaa
     3481 ccagatcgcc cggaaagtga acgccggtgg t
//
