LOCUS       CP052529                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0177 plasmid pB16KP0177-5,
            complete sequence.
ACCESSION   CP052529
VERSION     CP052529.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609871
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,715
            Genes (coding)                    :: 5,604
            CDSs (with protein)               :: 5,604
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 51 of 111
            Pseudo Genes (incomplete)         :: 61 of 111
            Pseudo Genes (internal stop)      :: 18 of 111
            Pseudo Genes (multiple problems)  :: 18 of 111
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0177"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0177-5"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(7844..9294,1..514))
                     /locus_tag="HJW83_29045"
     CDS             complement(join(7844..9294,1..514))
                     /locus_tag="HJW83_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJO48800.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(1995..2144)
                     /locus_tag="HJW83_29050"
     CDS             complement(1995..2144)
                     /locus_tag="HJW83_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJO48801.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2229..2486)
                     /locus_tag="HJW83_29055"
     CDS             complement(2229..2486)
                     /locus_tag="HJW83_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJO48802.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(2496..4181)
                     /locus_tag="HJW83_29060"
     CDS             complement(2496..4181)
                     /locus_tag="HJW83_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJO48803.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            4584..5003
                     /gene="exc2"
                     /locus_tag="HJW83_29065"
     CDS             4584..5003
                     /gene="exc2"
                     /locus_tag="HJW83_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJO48804.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            5309..5572
                     /locus_tag="HJW83_29070"
     CDS             5309..5572
                     /locus_tag="HJW83_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJO48805.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            5562..5861
                     /locus_tag="HJW83_29075"
     CDS             5562..5861
                     /locus_tag="HJW83_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJO48806.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5923..6300)
                     /locus_tag="HJW83_29080"
     CDS             complement(5923..6300)
                     /locus_tag="HJW83_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJO48807.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            6369..6548
                     /locus_tag="HJW83_29085"
     CDS             6369..6548
                     /locus_tag="HJW83_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO48808.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6576..7106)
                     /locus_tag="HJW83_29090"
     CDS             complement(6576..7106)
                     /locus_tag="HJW83_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO48809.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7113..7844)
                     /locus_tag="HJW83_29095"
     CDS             complement(7113..7844)
                     /locus_tag="HJW83_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJO48810.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     BGC             1..9181
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1314..1892
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7117..7844)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gcagccgcgc caacacaatc ccgacagcca gcccgcccgc gcaccaggca atcccgaaca
       61 ggttagccgg ctgcagcagg taataatctg cgtttgcgta gaggtccgga cgcaggacgg
      121 tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg
      181 ccgcgccggt cagccagtac gcgtagttcc agacatgtcg acgtgccctg accatccgcc
      241 cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg ccgtttatta tcgcgcttat
      301 agcgatcggc atactcccgt cgctccttat gactcagccc cacaaaacgc atccgtccgg
      361 cccaaagttc agccagaccg aaaaacaggc cgataactag gaaagccccg gccagaccgt
      421 aatactcctg gtacagccag ctgacccacg gataaatctg ccgggccagc tccggaatgt
      481 gccacttcca ggcaagcgaa tccgtattaa acatgttctc tctccttcag acgatggttt
      541 gccggcgtga tgtgcagccc ccgctgcaca agccgcggac atgccccaag gcatggcggc
      601 ggcggtcacg tccgtggtga ttgcgttcag ctttcaccac ggaggccagc ctctttccgc
      661 cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg tgtgagagga
      721 aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga gttcgccata
      781 gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgcca ggtgcgtcca
      841 gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg tgaatttctg
      901 gcgtggccgg cgtccagccg gacggagcca gaaagagggg gagagaccgt gcgtcggtgg
      961 cgtccagcca gcgtaagcag gggatatccc cctgaggtca gccacgccct cgcggggatg
     1021 acctgtattt ctccgtaagg agaaaaaagc aacagagata ggttaacgta aaatgttcag
     1081 cgaaaaaccg atatgattca cctatcctgg taatttatta tcagggaata taaagactta
     1141 atcaggggac aatatgccac atcagcgcgt ccggtgtccg tcttgtaggt gccggggaaa
     1201 ttgcgcctcc tcgctcactc ggtcgctacg ctccggccgt gagactgcgg cgggcgctgc
     1261 ggacacatgc aaagttaccc acagattccg tgaataagca ggggactaac atgtgaggca
     1321 aaacggcaag gccacgcagg tggcgttttt ccataggctc cgccctcctg actgagttca
     1381 cataaacaga cgctttttcg gtgcatctgt gggagccggt ggctcaacca tgaatcagtc
     1441 aggagtggcg aaacccgaca ggacttaaag atcatcaccg tttccgatga atcgctccct
     1501 cttgcgctct cctgttccaa ccctgccgtt tcccggatac ctgttccgcc tttctccctt
     1561 gcgggaagtg tggcgctttc tcatagctca cactctggta tctcggctcg gtgtaggtcg
     1621 ttcgctccaa gctgggctgt aagcaagaac cccccgttca gcccgactgc tgcgccttat
     1681 ccggtaactg ttcacttgag tccaacccgg aaaagcacgc aaaacgccac tggcagcagc
     1741 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
     1801 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
     1861 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca
     1921 ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     1981 tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga cgatggtgcc
     2041 accgttccac cctgaacatc ccggatatag tttgcctgac atgcgaccag aagaaagcct
     2101 gaaaaaatca atataaaaag aaaaacaacc tttacctttt tcatatacct tccctgtatt
     2161 taaaaacccc ggtattcatc cctgaacacc ggaagtctgt atttcaaatc atccttgata
     2221 acttttgctt accagttacc atcccggtaa acaaaggata caaagtaatc aaatttacta
     2281 atatcgatta catttttaaa gtgtggctgt aatatcgaaa cccatgattt ttcaacatca
     2341 aaccaaccat tattaatatt atcctttaaa ggcatcccca gactttcaat gacagaacca
     2401 tcatcaccga agtcttttga gtattcacca cctttaaact cttcggtttt cttatcaaac
     2461 caatgaatat gtaatttaag ccccatattt acctcttaaa gatatttttt aatgtttcgt
     2521 tttggatccg gccctttaac ctgcttaccc gtttttggat cgaatgcccc gaggtgttcg
     2581 ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg aatcccattc ataaatttta
     2641 cgctttttat caccatacca tcgggcccgc ttaccaccac caccttgttt aggggtttta
     2701 ggtttaccct ctttcaactc tcccaacccc ttaatatctt cagtcttggg atcaggaaaa
     2761 taatcatggc cgtaatcttt agttccctta cgaggttttt tcttttcctc atttaattta
     2821 ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggaaagc
     2881 gcagccttat ccctggataa ggattcattc atttcgttta aagatgtctg ggcagctttt
     2941 aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg
     3001 gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc ctctgcctct
     3061 tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggatcgc cttctctgtc
     3121 gatgcaatgc gactgtttaa ggtcgcaata tttttatctt cagcatccag ctcagctttc
     3181 gctttatcat actctctttt cagcccctct tccgggtgag cagcgtccca tgcctgctgg
     3241 cgacgctttt cttcctccag acgctgtttt acctgtgccg gagtaagaac atccgtcacc
     3301 gacacataga ccggtttctg tccgctctct ttcgggaaac gaataacggc ctcacgggag
     3361 ttaccaccgg ccgtaaaacc agccggacgt gaatcacctt tttcagcaat aatgcctttt
     3421 ggcggggctt tcgcggccgg aacaccttta ggtacgctca cctgcagaga cgggagaccc
     3481 ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac cacaggaaca
     3541 ctcattggcc ggccggtgac aaccgcaata tgctgccgct cgtccttcac aatatccgcc
     3601 acacgtttag tgacgctgac cgtcgcctgg tccagcggca gggagctgac cggcgtctcc
     3661 gttaccgtgt ctgccggcaa tgacgtcacg atttttgaca tcattttcgg atcatctttt
     3721 gctatctcag aaggcagcac tccgtaaatc gcaatccccc acagaccaaa cttaaacgga
     3781 cctttcaggg cagccagcac atcagcaaca gcggccgaca acgcatcgcc ggaaacggat
     3841 aaagccagtc cttcagcccc cggagtggcc agggcaggca gaccgaaggc catcgcggtg
     3901 gccgaagact gtccgccacc agagctcccg ctatgattgc cgctgttacc gccattacca
     3961 ccgggtttac tcccgccgtt accaaactcc cccgggttat agttatgaat atccccgtta
     4021 ggtgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc acctgatgaa
     4081 gggccacctt tacctgatgt accgttcaca ttcccactgg cctgaccacc attgtgtccc
     4141 agacctgaat tacccggacc tcgtccgtct ccgccactca tatacttttc ctctttgact
     4201 tttaaaacaa taaactaaaa taaaatactg tacatataac cactggtttt atatacagta
     4261 taaaaattac gctgccttgt ttttcatgtc aagtctgtaa atttgcgttt ttgtgaaaat
     4321 gatcaaaaaa acagccactc acaccatgcg caatcccctc cagatgcaac aaaaaccagc
     4381 acaaacggcg ccgttctccg gcctcagtat gttttactga aatcattata tgagatacca
     4441 gaaagccccc tccgggacag gaaaagaaac ggggggtctg gttcgggttg ttcggtcact
     4501 gaacacggtc agaaactgat gataagatag cctgcggata tataactcaa tgagaaaaaa
     4561 gaattaaagg taaggagacg ctcatgagac gttccggcag caaactgtgt cttatcgcca
     4621 tgataacagc gacagcaact ctcactgcct gtgctccaaa gggcagcgtg gaacagcata
     4681 cccggcatta cgtatatgct tcagatgacg gttttgatcc caatttttcc acccagaaag
     4741 ctgacacaac acgaatgatg gtgccttttt tccggcagtt ctgggatatg ggcgcaaagg
     4801 ataaagcgac cggaaaatca cggagtgatg tgcagcaacg cattcagcag tttcacagtc
     4861 aggaattttt aaactcactc caaggcacaa cacaatttgc gggtaccgat taccgcagca
     4921 aagacctcac accgaaaaaa tcaaggctac tggctgacac catttcagct gtttatctcg
     4981 atggctatga aggcagacag taagtggatt taccataacg cgtcattacg cgcaccgctc
     5041 agacgggctc agggcgtttc cggcggctac gggataaaac aggcaacaac ccccccggaa
     5101 aactgccgga aacgcgcccg ataaatttta tcctaatgca tgcctatgca cgcaactgca
     5161 tggcatatgt tttgacgtaa aatggatgcc gtaagccctt ttttaccgac tttctgcttc
     5221 atcagcctga ctgacacgac catattcttt cagaccgccc cctgctaaac ttccttcata
     5281 ttgccgcatt aacccgggag gctccgcaat gagcaacact acacactacg aaaacgccaa
     5341 ctttcttcgg gaactggctg aaagcctgcc ccgcatactg ccggaaggtg gcccggacaa
     5401 agctgcgctg ttgcagcgac tggctaacga agaactggcc caggcagagt atgaagatca
     5461 ggttcgcgcc aaagtgaccg ccgcccgtgc tgacacgcgt ccgggtatga ccacggaaca
     5521 actccgccag cgcctgcatg gccgttatca ggaactgcgc gatgccgtat gacgtggaat
     5581 ggaagcaggg agccatcgag gatgtaacga cgctgtttga ctatatcgcg gaaaattcat
     5641 ccctgtggga tgcgcagaac gttaccgacc gggttctcgc tgcggcggac aaactggccg
     5701 acttcccgcg actctatgaa gtggactcac gctatggcga ggacgttcgc cggatcagtc
     5761 tgatgggaca gcatgtcctc tatgatgttg atgaccagac cagaaaagtc cgggttctgg
     5821 ccgtggtcgg ccagcgccag aatccccata ttgttctcta ataaccggtg acggggacgc
     5881 aattcattgc gtggccgtcg ccggttggac aagcacagcg cgtcagcgtt ttccccacgg
     5941 ccaccaccgg cgcggtgttc cggccggggt ctctgctgtc tccggtttgt gttccagcag
     6001 tctcatggac tgcatttcct gccgtagccc ggccacctcc tccctgagtg cttttatctc
     6061 cgcgatcaac gcgtcggagg ccattttttc agtctcaccc gacagtctca actcgtcccg
     6121 ctcgtgagtc tcatcctgtc ttaactcacc gtacacccgg ataagctcgc tggtatccac
     6181 cacccggccg ccatcagctt ccgtacggta tgatacgcgg cctttcgcca tatcccgata
     6241 cagggaactt cgggctttac ccgtcagttc cgtcgcctgt ctcaccgtca cccacgccat
     6301 tgtctcaccc cgctattttt gagacagtat acctgacagg cgcacaccat gcccggaagg
     6361 aaagaatcat gacaaaacag gaaaaaacag ccctcaatat ggctaaattt attaaggagc
     6421 agtcgctgct attgctcgat aaactgaatg agctggatat ggataccccg gccgacatgt
     6481 gcgagcggct acacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg
     6541 aggaataaca gcaccggcat ggccggtgct ggcatttatt ttttgccgaa cggccaccac
     6601 cggcgggcag gtttgtcctc cggcagttca ggtaccggca cgttaaaata gcggctgtta
     6661 tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctca
     6721 ctcaccacgt caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc
     6781 tgcacaccgt tctcgatggc gaaccgctcc accttgcgat agaggtaatc acacagcttc
     6841 attcctgtgg gtttgttccg ttcccattgc ggatttttac cagtgaaaaa gtacgtggct
     6901 ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc
     6961 tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccata
     7021 tcttccagat gctcgctggt cggcatgatg cagcgctcgc cgttccggtc ctccgcgtct
     7081 tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac
     7141 gcaaacaacc gttcacgatc cagctctgcc gttttgcggg cctcgccggt ctgtgggttc
     7201 acgatttcat ccaccgacag gattgcccgc tgcaccctga cctgcgtcgc ccggtccggg
     7261 cagatgtacc agatttcatc gatgccgtta gctttgcggt tgaacagata cccggacacc
     7321 acttcctgat accggcgacg gctcttgacg gtgcgctcca cctcaaacgc caccgtttta
     7381 ccgtccagct ccgccagggc gtccggcact ttcatgcctt taagactcag acggtgcaga
     7441 atgcgccagc cggtggcccc tctggcttcc atatcaaggc gcagggattg cacggccagc
     7501 cggtgcggca gatgtgctgc cgacaccctt gacggctgaa acggaataac atccgccagc
     7561 ggttcgtcct cgtcgaacga gaacgccacg ccatgcggcg tcagccccca cacgctgacc
     7621 tgacggccgt ccaccggata tttggtccgg accagcagcc ctttcgccac caggctgtcg
     7681 aggaagcggt agaccggctg caccgccgca atccccagcc gccgcgcaac cagctccgcc
     7741 gtggaacaca gctcttcccg caaccagcgc agggtcagcc gggttttctc cgcgttccgc
     7801 gcctgtcgtt tcgcgtggct gacgttgtac agctccagcg ccatcagtaa atccccgcaa
     7861 aatcatcatc cgctttcggc ccgccgccgt cggccggggg ccggaactcc gccaggggat
     7921 cggttttcgc cagcaccggg aaacgctgca acgggatttc ccggcgctca acccgcagcg
     7981 gactgctgaa cgccatccgg gccacaccca gcccggtcat gaccgcgaac ccgtccggca
     8041 ggtgttgcag ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtccc
     8101 cgctgatatg ctccacgttc cccgcctccc ggctggcacg gcggcgctcc acgtccaccc
     8161 tgatttcccc ggtctgcccc gccgcccact gcgcggactc caggctggcc gcccggtaaa
     8221 accagcggat cggcgtgtta tccagtaccg tggttttcac aaattcagcg ggcagatcct
     8281 gcccgcactg cccgaaatcg cccagcgact ggtgcgcgag caacagattg cagtcccggc
     8341 tgcgcagcgt ccccagtgcg ttcagtacat acttcgacag cagatattta atttcatcga
     8401 gcatcacgct gacgtgtggc cactcccgga actcgtcccg cgcaatgatg atttgcgcgc
     8461 agcgggcaaa cagcattttt tgcacacgga tcaccgcctc gtcgtccatc gagccgataa
     8521 cgtacaggca gccgccgccg ttgataatcc ccgccacatc gatgccactg tccgtctgca
     8581 ggacgctcag gtcagcgacg ttttccagcc tagtgatcaa ccccttcacg tcctttttca
     8641 gggcctccgg cagcgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggaa
     8701 actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt
     8761 cgccggtctc gcccaggtta agcccggcaa tcagcaggtt tttcagcgcg tactggtcga
     8821 gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt
     8881 ccggacagtg cgcccggaag acgtgcggcg cccatgcgtc ccctttgggg tcgaagtaca
     8941 ccaccgcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcacgctct
     9001 tgccgctccc gggcaggccg aggatctgaa tgttggtctt gcgccaggta tcacgcggaa
     9061 ccagcaccgg cttgccctgc tcatccttac ccagaaacac cgcctgctcc tgccgggcct
     9121 gctccgagga aaaatcggcc agcgcattaa ccggtaccgg ctccccgaaa tgcaggtggc
     9181 gcacgtcggt catctctcct gtccgctggc tgctgcgccg gctctcctga ttcagtttcg
     9241 cgttcaccgc ctcgtcagca gccgcaaaat gaccgtcaag cagcaggaac gcca
//
