LOCUS       CP052509                2496 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0226 plasmid pB16KP0226-4,
            complete sequence.
ACCESSION   CP052509
VERSION     CP052509.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609875
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:40:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,704
            CDSs (total)                      :: 5,581
            Genes (coding)                    :: 5,471
            CDSs (with protein)               :: 5,471
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 52 of 110
            Pseudo Genes (incomplete)         :: 54 of 110
            Pseudo Genes (internal stop)      :: 18 of 110
            Pseudo Genes (multiple problems)  :: 13 of 110
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0226"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0226-4"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(243..470)
                     /locus_tag="HJW87_28510"
     CDS             complement(243..470)
                     /locus_tag="HJW87_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QJL96962.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1699..1890
                     /locus_tag="HJW87_28515"
     CDS             1699..1890
                     /locus_tag="HJW87_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL96964.1"
                     /translation="MTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQILSETEPAA
                     RAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(2188..2373)
                     /locus_tag="HJW87_28520"
     CDS             complement(2188..2373)
                     /locus_tag="HJW87_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL96963.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1393..1562
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          2054..2141
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(886..1468)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtggtttt tgtcatggtt ccgtcagaaa cgcgctgagc gcgtctgagg cggtgcgggc
       61 aacggatgtt atggtaaatt cttgggggct tgattataaa ataccgcact tcctcttatg
      121 tcttgtgctt tttagacaac cctgaaagct tttagctttg gtgatgcaat ttgctgggat
      181 tttggcggag aaccacaggt aaagaaaaag gccacattag cggccttttt cggagaatat
      241 gctcagtgcg gaatttcgga gacttcttta acgtcggttt tattgatctg ctgtttgaca
      301 ccattcgcgt ctgtatagcc aagcaggcct gagtctgatt ctttaggttt gccgtcgctg
      361 acaatagtgc gaccgtcatt ggtgtgtatg gcatagcttg tgcgcgtgca gccggtgagg
      421 gctgaaagcg caactgccgc tgcgaacact gaaacaaaaa tctttttcaa agccagctcc
      481 ttttatccat atcagttttg gtcactaacc atctaactat aggttatttt ttatgtgaaa
      541 agaggcttga tggtgggctg tatatcgagt taggcgcgag gtgctatggt caaagtgtgg
      601 tgtcaagcgg ggaggaggct tatatgagat gtaatccagc agatacatat accaagatga
      661 tttaagtatt cctctttgtg aggttggatg tctaatttat aaaggatctt cttgagatcc
      721 ttttttttcg cgcgtaatct cttgccctgt aaacgaaaaa accaccctgg caggtggttt
      781 ttcgaaggtt aggtaatcct ggcagatccc ctaaccgtgg taacagtctt gtgcgagaca
      841 tgtcaccaaa tttgtccttt cagtgtagcc tcactaaggc cgccacttca agaactcttg
      901 atacatctct cgcacatcct gtttaccaat ggccgttgcc aatggcgatt agtcgtgtct
      961 ttccgggttg gactcaagtt gatagttacc ggataaggcg cagcggtcgg actgaacggg
     1021 gggttcgtgc atacagtcca tcctggagcg aactgccttc ccggaactga gtgtcaggcg
     1081 tggaatgaga aaccgcggcc ataacagcgg agtgacaccg gtaaaccgaa aggcaggaat
     1141 gcgggggagc acgagggagc caccaggggg aaacgcctgg tatctttaag ccgcatcggg
     1201 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
     1261 tggaaaaacg gctttgccgt ggccttatgc ctttttcgct aagaaccttc ctggcattct
     1321 ccaggaaatt tctgctctgt acgtaagccc gtaccgctcg ccgcagccga acgaccgagc
     1381 gcagcgagtc agtgagcgag gaagcggaat atctcctgta tcacatattc tgctgacgca
     1441 ccgatgccgc ctttttctcc tgccacatga agcacttaac tgacatccgc atccgtgcca
     1501 acatattaag ccagtataca ctccgctagc gctgcgtgac tggtccggcg ctgcgcccgg
     1561 aacccgcctg taccggttaa gcagccgttc tggtctgact gcgatttttt tcattcctgc
     1621 ccgctaccct gtaaaccttt cttctgcgtt gccgttaaca ggaggtaagc catgtttggt
     1681 ttttcctccg gagccctgat gacttttaat gatgatgagc gacacctgct tgtgtccgtt
     1741 gtttcgggct ggctccggcg tgccgaaggg gatgccggag ccatgatgct ggatgcatac
     1801 cggcagattt tgtctgaaac cgaaccggcc gcaagggcgg tcatgctcga atttcttgaa
     1861 tcagttcgca ttcattacgt ttcgtcctga gcatttcctg aagcatttcc tgaatttttg
     1921 tccatactca atccaggcgg ccgtcacgga cggtggtcgt cgttccatga tgctgttatg
     1981 gaagattgag agtaatctaa cttgattgag ttccgggcgt gtcgcccggt tttttttgct
     2041 gtttcccggt gtccgctccc ggacaggatg tgcatttgta ataccgcgca cgcggcggca
     2101 tcacaaatac cccgttcagg gctgcgcccc gacaccccgc catcgcctca cggccgcgca
     2161 ctgaattgcg cacccgttca ccgattttca cgcatctttc tcgcctatct gtgcgttcag
     2221 catggcgtaa agcgcttcag cctgatcgtg cagatgctca cagcagccgg ctgcctcatc
     2281 cagatccatc tgttcgagcc gttcgaggag gaggagtgtc tgtgagcgga taaatttggc
     2341 catgttgagg gcggcggatt cctgtctggt cactggtgat tttccccgtc tggtgatgat
     2401 ttttgcgtga aaagagcatg ccgtcattct ggtgcatagt catgcatgcc gttaaaattt
     2461 aacaggcgcg tttctgacgg gttccggggt ggtttg
//
