LOCUS       CP052508                3991 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0226 plasmid pB16KP0226-3,
            complete sequence.
ACCESSION   CP052508
VERSION     CP052508.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609875
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:40:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,704
            CDSs (total)                      :: 5,581
            Genes (coding)                    :: 5,471
            CDSs (with protein)               :: 5,471
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 52 of 110
            Pseudo Genes (incomplete)         :: 54 of 110
            Pseudo Genes (internal stop)      :: 18 of 110
            Pseudo Genes (multiple problems)  :: 13 of 110
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0226"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0226-3"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(456..728)
                     /locus_tag="HJW87_28485"
     CDS             complement(456..728)
                     /locus_tag="HJW87_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL96958.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            complement(1532..1954)
                     /locus_tag="HJW87_28490"
     CDS             complement(1532..1954)
                     /locus_tag="HJW87_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL96959.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1954..2145)
                     /locus_tag="HJW87_28495"
     CDS             complement(1954..2145)
                     /locus_tag="HJW87_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL96960.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2102..2511
                     /locus_tag="HJW87_28500"
                     /pseudo=""
     CDS             2102..2511
                     /locus_tag="HJW87_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            complement(2892..3866)
                     /locus_tag="HJW87_28505"
     CDS             complement(2892..3866)
                     /locus_tag="HJW87_28505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL96961.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     Typing          2194..2665
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 acaagtctgg acgctcatcg tgaagtttga tttttttcat agagcacctc tcattaattg
       61 ttcctttgca agcccatgct tgcttagcgc tgaatatagg gcataaaccc agcactcaca
      121 acaacaaaaa tgcttaaaat gataaaatca acccatgtat tgacattggg tgaaaaaaaa
      181 agtataatgc actggtagcc aaaggggcgg ggcttcgctt attccttagg aataagcgaa
      241 gccccgcccc tttggctacc ataattaaat gattgaattt aaaggatttt tttacaataa
      301 catccttcct ctcaatagtc atataaacat gccccatgta ttcgtcattc tggatttttc
      361 aaaagaatga atgcgtgcat ttaccataac gcctcatttc acgcagatat gaaaaagccc
      421 gcacaaggcg ggcttcagac tcaacagata cggaatcact ctgtgaaaaa ctgctttgcc
      481 atggggtgat tctgcatgat ctcgtcacgt tcttccgggt caaagcaccc acagacaccc
      541 agcagaaccg gctctgtcag cattccggca cagcatcccg tggtgacctg tattttacct
      601 ttccggtcat acccaaaaac ggtgtaggtc acatcatcct catcatagac ttcatgacag
      661 atggagcatg agtccgggta ttttttgatg agtgcattaa tttcttttac ctgagcgtca
      721 gtagccaaaa catctccctg agtcactgat tatcacaatc cgctgactct aacacagact
      781 caggcatccg ccgttgattt accataacgt cccattacac gcaccgctaa aacggcctca
      841 gaacgattct ggggcgtaac ccattaaatc ctacgtctta ccccctgaaa acgcctcaaa
      901 atcgctccag ggcgttttac gggcatgaat cgttatgcac ttgactgact gtctggggat
      961 ttcagcctga attcaggcca ggcaactcaa aacccgcacc aaaccagact gaaaacggtg
     1021 aacgggtgcg caatttattg cgtggccgtg aagcgttgcc ggcgcagccg gaaaaccggt
     1081 tttcagcacg ttcaggaatc gggtttgtcg agaccagcaa tcacattatg gcaagtcgcc
     1141 ggacgactca ccacacgatt caccggcgat tcgactcgtg acttgactct agagtcgcca
     1201 tggtcaaaaa gccaccaacc cggcaagtcg actctagagt caagtcatca taacgtactg
     1261 attatatggc gtgattcaca tatgaatcgt gggcaagtca tcggcgattc gccatagagt
     1321 cgtccacgac tcggattcac cttacaaaaa ataaaattgc gtaatttaaa atagataact
     1381 gattgaaaat aaaggtctat ctgcaaaatt ttaattttgc gtagtgtgtg ggtattttta
     1441 gtggtgagga gcgttcaggg atctgacgat ttttgggaag gttcaggaac tccccggaat
     1501 ggccggagag caaaatggaa gcatggcggg atcaggattt tttccagaat ttccaccacg
     1561 gggaatgggg tggtgaagaa ggttcgtgct tgtgctccag aagctgcata gcctgacgaa
     1621 gggaatcaat atgctcatct cgagccttaa cagccccttt aagaccatcg ttttcagctt
     1681 caagtaaagc gattctatgt tgtaaatcat gtaaaccaac gctgttaata cctgtagatt
     1741 ctgcgtcact ttttacatca tattttacag tatcgattac accatcaaga gaaccaaaca
     1801 ctctaattag ctctgaagta tcaataactt taatagcttt cccgtgcttt accacagagg
     1861 tgtaacttaa cttcccactg ttaatcatct tgtaaattgt ggggcgacta accttaacaa
     1921 gccgtgccgc ttcactaata gacactatgg ccatcagtta ccatcctgta attcatccag
     1981 tctcgaggac agtgtacgga aaagatgctc cgcatcatca tggagtttct cacaaaggtc
     2041 ggcctcgaca tcgagatcaa gctcgttaag cttatccagc aggagcagag actgattctg
     2101 gatgaattta gccatgttca gcgctgtttt ctgttgtttt gtcataagaa gcctcagagc
     2161 gtcagtgctc aatgtcaaac tagtctcttt tccgcttcct cgctcactca gtcgctacgc
     2221 tccagccgtt cggctgcggc gagcggcagg agcgtgtaca taaaaacgta ggcaaccaag
     2281 atatgaggca gaaaagcaaa ggccgccgaa cggctgtttt tccataggct ccgcccccct
     2341 gacaagcatc gcggaaacgg acgctcaaat cagtggtggc gaaacccgac aggacttaaa
     2401 gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt
     2461 ttaccggtgt cattccgctg ttatggccgc gttttctcat tccatgcctg atactcggct
     2521 ccgggaaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact
     2581 gctgcgcccg ttccagtaac tatctcttga gtccaacccg gaaagacacg acttatcgcc
     2641 attggcagca gccactggta attgatttag aggagttagt cttgaagtta tgcgccagtt
     2701 aaggctaaac taaacgaact gttttggtga ctgcgctcct ctaagccagt tacctcggtt
     2761 cagaaacttg ccggttactg aaccttcgaa aaaccacctt gccgggtggt tttttcgttt
     2821 gaggggtaga gtacgctgca cctcaaagga tcgtcaagga tctttttgga attcatagag
     2881 ctagccactg cctatttgat agccaccttt aattcctcca gctcagcgtc agtaaacaac
     2941 ccttcaagat cattatcttt atttcctatg accttggttc ttattttttc ttgcctgcga
     3001 atgaattcag gatctgtaac tttaatagtc agagccctgc gaatcatatc cttaatatcg
     3061 gagttatcag cttggcaacg acgcaaaata tattgatagc aagccttatt gtaaataagt
     3121 atagggttat cctcattact cataagtaac tcatccgtaa ttgacagagc atcatcaata
     3181 cgatcaagtc ttttcaaaat ataagcttta agcactaaga gcttgtcgta gtcatcctta
     3241 gctacatcct ctcttttaac aagagacaat gattccgtta cagcatctaa tgcctcagtt
     3301 agttttttct taacagaaag agcctcatcc ggaatggaca tcattgattc tgctttatcc
     3361 acagctgaaa tggctttaaa gtatcctagc tcgaattttg ttgttttctt atccatttca
     3421 agctgttcta tcagctcctt gctcctatca agctcatctt taaactcatc ttgttcagcc
     3481 tccaaatctt gttgcttttt atctataccg tctacttgct cttttaatac tttgttggca
     3541 agaccatcca gtaatgcgta cccaagatac gcagagatcc ccgaaacaga aataagaagc
     3601 aatatttgtt ctatgaactt agttccacca ccatcagcca caggaaatat tatacttgag
     3661 taatcaacat gaagaaatga tgatatgaaa ggaacacaaa taaatgcgga tgctatacca
     3721 aatctataaa cactagctct tttattattc tcatccttat cttttgaaaa tttattttgg
     3781 gagtctcggc aacctctgga atagctcccc ataacaccac caaaacaaat tagagatgca
     3841 acaaacaata ggttaaaaaa agccatgaga gaatccttgc gcaaagcaaa caataaaact
     3901 ttgttcaggt aagttaagat aagctcaatc atatactaac caataacaaa aaaggcagcc
     3961 ttggctgcct ttttctgctg acttgacact t
//
