LOCUS       CP052501                3991 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B17KP0021 plasmid pB17KP0021-2,
            complete sequence.
ACCESSION   CP052501
VERSION     CP052501.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609877
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:43:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,668
            CDSs (total)                      :: 5,545
            Genes (coding)                    :: 5,431
            CDSs (with protein)               :: 5,431
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 55 of 114
            Pseudo Genes (incomplete)         :: 56 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 12 of 114
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B17KP0021"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB17KP0021-2"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(549..821)
                     /locus_tag="HJW89_28305"
     CDS             complement(549..821)
                     /locus_tag="HJW89_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL86175.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            complement(1625..2047)
                     /locus_tag="HJW89_28310"
     CDS             complement(1625..2047)
                     /locus_tag="HJW89_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL86176.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2047..2238)
                     /locus_tag="HJW89_28315"
     CDS             complement(2047..2238)
                     /locus_tag="HJW89_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL86177.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2195..2604
                     /locus_tag="HJW89_28320"
                     /pseudo=""
     CDS             2195..2604
                     /locus_tag="HJW89_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            complement(2985..3959)
                     /locus_tag="HJW89_28325"
     CDS             complement(2985..3959)
                     /locus_tag="HJW89_28325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL86178.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     Typing          2287..2758
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 ctttgttcag gtaagttaag ataagctcaa tcatatacta accaataaca aaaaaggcag
       61 ccttggctgc ctttttctgc tgacttgaca cttacaagtc tggacgctca tcgtgaagtt
      121 tgattttttt catagagcac ctctcattaa ttgttccttt gcaagcccat gcttgcttag
      181 cgctgaatat agggcataaa cccagcactc acaacaacaa aaatgcttaa aatgataaaa
      241 tcaacccatg tattgacatt gggtgaaaaa aaaagtataa tgcactggta gccaaagggg
      301 cggggcttcg cttattcctt aggaataagc gaagccccgc ccctttggct accataatta
      361 aatgattgaa tttaaaggat ttttttacaa taacatcctt cctctcaata gtcatataaa
      421 catgccccat gtattcgtca ttctggattt ttcaaaagaa tgaatgcgtg catttaccat
      481 aacgcctcat ttcacgcaga tatgaaaaag cccgcacaag gcgggcttca gactcaacag
      541 atacggaatc actctgtgaa aaactgcttt gccatggggt gattctgcat gatctcgtca
      601 cgttcttccg ggtcaaagca cccacagaca cccagcagaa ccggctctgt cagcattccg
      661 gcacagcatc ccgtggtgac ctgtatttta cctttccggt catacccaaa aacggtgtag
      721 gtcacatcat cctcatcata gacttcatga cagatggagc atgagtccgg gtattttttg
      781 atgagtgcat taatttcttt tacctgagcg tcagtagcca aaacatctcc ctgagtcact
      841 gattatcaca atccgctgac tctaacacag actcaggcat ccgccgttga tttaccataa
      901 cgtcccatta cacgcaccgc taaaacggcc tcagaacgat tctggggcgt aacccattaa
      961 atcctacgtc ttaccccctg aaaacgcctc aaaatcgctc cagggcgttt tacgggcatg
     1021 aatcgttatg cacttgactg actgtctggg gatttcagcc tgaattcagg ccaggcaact
     1081 caaaacccgc accaaaccag actgaaaacg gtgaacgggt gcgcaattta ttgcgtggcc
     1141 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgggtttg
     1201 tcgagaccag caatcacatt atggcaagtc gccggacgac tcaccacacg attcaccggc
     1261 gattcgactc gtgacttgac tctagagtcg ccatggtcaa aaagccacca acccggcaag
     1321 tcgactctag agtcaagtca tcataacgta ctgattatat ggcgtgattc acatatgaat
     1381 cgtgggcaag tcatcggcga ttcgccatag agtcgtccac gactcggatt caccttacaa
     1441 aaaataaaat tgcgtaattt aaaatagata actgattgaa aataaaggtc tatctgcaaa
     1501 attttaattt tgcgtagtgt gtgggtattt ttagtggtga ggagcgttca gggatctgac
     1561 gatttttggg aaggttcagg aactccccgg aatggccgga gagcaaaatg gaagcatggc
     1621 gggatcagga ttttttccag aatttccacc acggggaatg gggtggtgaa gaaggttcgt
     1681 gcttgtgctc cagaagctgc atagcctgac gaagggaatc aatatgctca tctcgagcct
     1741 taacagcccc tttaagacca tcgttttcag cttcaagtaa agcgattcta tgttgtaaat
     1801 catgtaaacc aacgctgtta atacctgtag attctgcgtc actttttaca tcatatttta
     1861 cagtatcgat tacaccatca agagaaccaa acactctaat tagctctgaa gtatcaataa
     1921 ctttaatagc tttcccgtgc tttaccacag aggtgtaact taacttccca ctgttaatca
     1981 tcttgtaaat tgtggggcga ctaaccttaa caagccgtgc cgcttcacta atagacacta
     2041 tggccatcag ttaccatcct gtaattcatc cagtctcgag gacagtgtac ggaaaagatg
     2101 ctccgcatca tcatggagtt tctcacaaag gtcggcctcg acatcgagat caagctcgtt
     2161 aagcttatcc agcaggagca gagactgatt ctggatgaat ttagccatgt tcagcgctgt
     2221 tttctgttgt tttgtcataa gaagcctcag agcgtcagtg ctcaatgtca aactagtctc
     2281 ttttccgctt cctcgctcac tcagtcgcta cgctccagcc gttcggctgc ggcgagcggc
     2341 aggagcgtgt acataaaaac gtaggcaacc aagatatgag gcagaaaagc aaaggccgcc
     2401 gaacggctgt ttttccatag gctccgcccc cctgacaagc atcgcggaaa cggacgctca
     2461 aatcagtggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct
     2521 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     2581 cgcgttttct cattccatgc ctgatactcg gctccgggaa ggcagttcgc tccaagctgg
     2641 actgtatgca cgaacccccc gttcagtccg actgctgcgc ccgttccagt aactatctct
     2701 tgagtccaac ccggaaagac acgacttatc gccattggca gcagccactg gtaattgatt
     2761 tagaggagtt agtcttgaag ttatgcgcca gttaaggcta aactaaacga actgttttgg
     2821 tgactgcgct cctctaagcc agttacctcg gttcagaaac ttgccggtta ctgaaccttc
     2881 gaaaaaccac cttgccgggt ggttttttcg tttgaggggt agagtacgct gcacctcaaa
     2941 ggatcgtcaa ggatcttttt ggaattcata gagctagcca ctgcctattt gatagccacc
     3001 tttaattcct ccagctcagc gtcagtaaac aacccttcaa gatcattatc tttatttcct
     3061 atgaccttgg ttcttatttt ttcttgcctg cgaatgaatt caggatctgt aactttaata
     3121 gtcagagccc tgcgaatcat atccttaata tcggagttat cagcttggca acgacgcaaa
     3181 atatattgat agcaagcctt attgtaaata agtatagggt tatcctcatt actcataagt
     3241 aactcatccg taattgacag agcatcatca atacgatcaa gtcttttcaa aatataagct
     3301 ttaagcacta agagcttgtc gtagtcatcc ttagctacat cctctctttt aacaagagac
     3361 aatgattccg ttacagcatc taatgcctca gttagttttt tcttaacaga aagagcctca
     3421 tccggaatgg acatcattga ttctgcttta tccacagctg aaatggcttt aaagtatcct
     3481 agctcgaatt ttgttgtttt cttatccatt tcaagctgtt ctatcagctc cttgctccta
     3541 tcaagctcat ctttaaactc atcttgttca gcctccaaat cttgttgctt tttatctata
     3601 ccgtctactt gctcttttaa tactttgttg gcaagaccat ccagtaatgc gtacccaaga
     3661 tacgcagaga tccccgaaac agaaataaga agcaatattt gttctatgaa cttagttcca
     3721 ccaccatcag ccacaggaaa tattatactt gagtaatcaa catgaagaaa tgatgatatg
     3781 aaaggaacac aaataaatgc ggatgctata ccaaatctat aaacactagc tcttttatta
     3841 ttctcatcct tatcttttga aaatttattt tgggagtctc ggcaacctct ggaatagctc
     3901 cccataacac caccaaaaca aattagagat gcaacaaaca ataggttaaa aaaagccatg
     3961 agagaatcct tgcgcaaagc aaacaataaa a
//
