LOCUS       CP052492                5627 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B17KP0069 plasmid pB17KP0069-2,
            complete sequence.
ACCESSION   CP052492
VERSION     CP052492.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609879
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:11:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,397
            Genes (coding)                    :: 5,266
            CDSs (with protein)               :: 5,266
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 57 of 131
            Pseudo Genes (incomplete)         :: 72 of 131
            Pseudo Genes (internal stop)      :: 24 of 131
            Pseudo Genes (multiple problems)  :: 22 of 131
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B17KP0069"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB17KP0069-2"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(join(5514..5627,1..120))
                     /gene="mbeD"
                     /locus_tag="HJW91_27540"
     CDS             complement(join(5514..5627,1..120))
                     /gene="mbeD"
                     /locus_tag="HJW91_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJL37684.1"
                     /translation="MTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALT
                     NSDRVCQHLSSQVDSLRVQVESLSRNVSRLMR"
                     /name="mbeD"
     gene            complement(506..1354)
                     /locus_tag="HJW91_27545"
                     /pseudo=""
     CDS             complement(506..1354)
                     /locus_tag="HJW91_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1344..1670)
                     /gene="mobC"
                     /locus_tag="HJW91_27550"
     CDS             complement(1344..1670)
                     /gene="mobC"
                     /locus_tag="HJW91_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001715145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="QJL37679.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQIDSLTPTERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="mobC"
     gene            complement(1898..3658)
                     /locus_tag="HJW91_27555"
     CDS             complement(1898..3658)
                     /locus_tag="HJW91_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="QJL37680.1"
                     /translation="MARDDFNSDTRTVGLARLANSLEETYGSFINMENTNSKGEARREV
                     LITRCIAAHAVRMYGDELDPEVAAASVCDGSDDGGVDAVYVNPSSKKLILVQSKYIKDG
                     NGSVTIKDFGRFKDACAKVIANDLNSFNDRFLEHKEAVTTAITSSSYKCICVFIYSGTQ
                     GLSSEVIGEIDFWEKSQNKALLFRDLESRDEYTILFETPNVTDISTSLSTQSTGAIDIS
                     NVILESYGEITEPYGAIYGTIAARVIHDWWKKYKYALFEKNIRSVLGDSTSVNAGIMST
                     IENSPENFWYYNNGITATYEEVDESIANAGASRKIGIFGFKKLNIINGAQTVSSIGAIF
                     DSIPEEKKDKARVMIRFINAKDPVFLGDVTKYNNTQNRVTGRDFTTQRPEQQKIQREIN
                     FVGGYTYKLLRQEDSGIAASNVIDIDDSLNALICASKKPQLIATLKSNRGRFFDTTEGN
                     LYEQVFNPRNPPSGLSVINAVNLYRVCFDVLKDTITNASNSSEEEYSKIPSLLTHGNYV
                     FISILMGKIQPTNTVNGINTYDKNSVSVDTLELAKSIFEYIQSNYPNSYLARFFQNKEK
                     VEQIITHFSA"
                     /name="AIPR family protein"
     gene            complement(3645..4547)
                     /locus_tag="HJW91_27560"
     CDS             complement(3645..4547)
                     /locus_tag="HJW91_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="QJL37681.1"
                     /translation="MHNAALALFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSVRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFASPLE
                     DKEVMGIAKSISKWTEKKFTASSFKEYVINSHKSDIQSKRGRLGGLKSKGGGRPTINSP
                     WKDMGISKSTYYRNIHGKR"
                     /name="replicase"
     gene            complement(4740..5024)
                     /locus_tag="HJW91_27565"
     CDS             complement(4740..5024)
                     /locus_tag="HJW91_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL37682.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5026..5358)
                     /locus_tag="HJW91_27570"
     CDS             complement(5026..5358)
                     /locus_tag="HJW91_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJL37683.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAVLKSLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5419..5598)
                     /locus_tag="HJW91_27575"
     CDS             complement(5419..5598)
                     /locus_tag="HJW91_27575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL37678.1"
                     /translation="MSALEQSGGQLARAGRELKQERQPLDALIAQHDRARAEKLREQQP
                     EPQKKPTRYYGPSM"
                     /name="hypothetical protein"
     Typing          complement(3..1354)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.118"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggtagcctcg aacatgtgct gcaagctgct gtagctgtcc tgccacgcct gctgctgttg
       61 ctcgtagctt gtctgtaagc tctctaaggc gctcagaagc tgtttttcca tctcggtcat
      121 ggttttggtc atcctcggtg ttaaaaacgc gctgtggcgt ttcagggcgc tctgacggag
      181 ttatttcgcc gtgtatttca ctacggtgtt gtcggtcgtc gatgtatagg tttccacgcc
      241 gtgcgcctgc ttcggcagca ccaccatgta ggtattccgg tgcttgtcgt cgctgtagat
      301 cgccacgccc gcaccgcttt tcgctttcag gctctccagc tgttggctgt agctgtccat
      361 ctcgctcagg cgcgaggtga tgatcgtgcc ctgaaaccag attatcccgc ccatgatgcc
      421 gatcagcagg gcgaagcaga tgaatatcca cgccatcgcc gttgagacag aagctctgag
      481 catgcccgtc agtctgccgt tctgctcgcg gatagcgttg ctgatgctct gcgagcttga
      541 ttgcagttcc gcgctgatag acgtctccag cctcgcgaac tcgcgcttca cgctctgctc
      601 ggtatcctgc gcttgctgct tcgatttctc ctcgaagccc ttcgccaaat ttaaaatctc
      661 gctcataaat cattcctttc agccggatat ttcggccgcc gtccggatcg gcaatgctga
      721 tgctgctttt cgtttcccgc gccacggtca ggccatagct ttccagttgc gtcaccacgt
      781 cctgccggct gcggatcatg ccctgctgca tcaggttcat cagcccagca gtaattttct
      841 ctgccgcctg ctgtttgtcg cggggcagat cgctggcctg cgtcagcggg cggcgataag
      901 tcgggtcgtt cgggtcgcgc agcgccagcc ggtcgttagt cagggtctgc caggcgttga
      961 cgcggggacg gtcggcgcgg tcaaagtagg gttgcaggcg ttttccactc tgcaattcga
     1021 tgttcgggat gacaaaattc agctcaagcc gtcccttgtc gcgatgctct acccacaggc
     1081 aggcgtactg gtcgcggtcg aggccggtca gcagcgtgtg ctcccattcg tccatcaggc
     1141 gcgatttctc ggcgtctgca atgtcaggtt cctggaatga aagcacgcca gaagtgtagg
     1201 cgcgggcaaa gtcgcagccg tcgatcagct cgcgcacgtg ttcaggattc ccgcgcagta
     1261 cgctggcctg ttcgcgctgc cggtctttac ccagcaggta atccaccgga ccactgccgg
     1321 aacctgcacc gcgtgagtga attttaacga tcacggttct gctccagcag gtcgccaagc
     1381 tgccggtcaa ggctggtcag tacgacaagc agcgaggcac gttcagtcgg cgtcagggag
     1441 tcaatctggt tcaggcggcg ggcaatttgg ttcaggttgt taccaatccc actgacctgc
     1501 cgcagcagtt cgggtgccac ctcaggcaca cggcgcttgc gtgaggtgtg ttcacccaga
     1561 ccaagctgac gtagccactc cgcaaggtga gtccggtcgc agcgatcgag cagtcgctga
     1621 tgctcagttt cagtgagccg gattttgatc tctttcgttc gcttttccat aggctccgct
     1681 gagaaaaaat tcgcaccact gtgagaattt ttcgcagtgg atgccggggg tttcgggggg
     1741 cggcgagccc cctgaaccag tcacgggcgg cacctcggta gaggggacgc tgtgtgtact
     1801 ggcttaaagt atggtgcagt atgtcagatc ctgtgctggc tgcaaaaaaa aagccctctt
     1861 tagagagggc tgttatctga tataggcttg tttagtctca cgctgaaaag tgagtgatga
     1921 tctgctctac tttttctttg ttctggaaaa atctagccag ataactatta ggataattag
     1981 actggatgta ttcaaatatg cttttagcta gttcaagagt atcaacagaa acgctattct
     2041 tgtcataggt gtttatgcca ttaactgtat ttgtgggttg tattttaccc attaaaatac
     2101 tgatgaatac atagttacca tgcgtcagca atgatggaat tttactatac tcttcttctg
     2161 atgagttaga tgcgttagta attgtatctt ttaaaacatc aaagcacact ctgtacaaat
     2221 tgacagcatt gataacagaa agaccggatg gcggatttct tgggttgaaa acttgttcgt
     2281 aaagattgcc ttctgttgta tcaaaaaaac gacctctgtt agacttcaac gttgcaatca
     2341 attgaggttt ctttgatgcg caaattagtg catttaatga gtcatctata tcaatcacat
     2401 tagaagcagc tatccctgaa tcttcctgtc gcaagagctt atatgtataa cccccaacga
     2461 aatttatttc tctctgtatt ttttgttgct caggtctttg tgtcgtgaaa tctctgccgg
     2521 taactctatt ctgagtattg ttgtacttag ttacgtcacc taaaaaaact ggatctttcg
     2581 catttataaa cctaatcatt actcgagctt tgtctttttt ttcctcaggt atactatcaa
     2641 atatagctcc aatagagctt acagtttgcg ccccatttat tatatttaac tttttaaagc
     2701 cgaatattcc aattttacga gatgctccag cattagctat gctctcatct acctcttcat
     2761 acgtcgcagt aatcccatta ttataatacc aaaaattttc tgggctgttt tcgatggtag
     2821 acataattcc tgcatttact gatgtcgaat cacccaatac actccttatg tttttctcaa
     2881 aaagcgcata tttgtatttt ttccaccaat catgtatcac ccttgcagca atcgttccgt
     2941 agattgcgcc gtaaggttca gtgatctcac catacgactc taatattaca ttgcttatat
     3001 ctatagctcc ggtagattga gtactcaagc tagtagatat gtcagtaaca ttaggtgttt
     3061 caaatagtat tgtatactca tccctagatt ctaaatccct aaacaacaga gctttatttt
     3121 ggctcttctc ccaaaaatca atctctccaa taacttctga gcttaatcct tgggttcctg
     3181 agtaaataaa aacgcatata catttatagc tactgctagt gatagcagta gtcactgctt
     3241 ctttgtgctc taaaaaccta tcattaaatg aattgagatc gttagctata acttttgcac
     3301 atgcatcttt aaaacgtcca aaatctttta ttgttacaga cccattccca tcttttatgt
     3361 attttgactg taccaaaata agttttttag agcttgggtt tacataaaca gcatcaactc
     3421 ccccatcatc gctaccatcg caaactgatg cagcagcaac ttcagggtcg agttcatcac
     3481 catacatcct cactgcatgc gcagcaatac atcttgtgat cagaacctcc cttctagcct
     3541 cacctttaga gttggtattt tccatattaa taaaagaacc ataggtttct tctaggctat
     3601 tagccaacct tgcaagtcca acggttctag tgtcgctatt aaaatcatct cttgccatgg
     3661 atattcctat aataggtcga tttactaatc cccatatctt tccacgggga gtttattgtt
     3721 ggccgcccgc cacctttgct ttttaatccg cctaagcggc ctctcttaga ttgaatatca
     3781 ctcttatgag agttaatgac gtactcttta aatgaagatg cagtaaattt tttttcagtc
     3841 cattttgata tgctttttgc aatgcccatt acttctttat cctctaaagg agaagcaaat
     3901 tgcaggttat aggcgctggc acgttcgtag caggcttgca gccactgctc atactcaggc
     3961 cagccctgac ggatggcgcg gtatgcccac ttacgggttt tgtcgaacag ggtgcagtta
     4021 cgacctaatc cgtagtcggc aacgatttct ttatcgttcg cagcgttcag gtcaaggaaa
     4081 tcggccagcc agtccagtgt gtagagttcc ggctgccaca cggcaatttt ccagtgcccg
     4141 tggttcggat ttttacagat tagccccgaa taccccgcat cagcctctaa ttttttacgt
     4201 agcgcattct ccacggcggc ggcgtagcgc agcggcttca tttttccgtc cggcgcggta
     4261 cgtacagagg ttttcagcgc gtacagcagg tgcgcgtgtc cgttgtcggg gttggtaatg
     4321 gtcagcgtgg gcgcgggcgc gttgcggtcg ctccagtcga tggcggcacc ggcgcgatca
     4381 acatcgaatc ccagccagaa catggcgtga ggctgattga actggatgta tttcgccaga
     4441 atggcgcgtt ctttaccggc aatgcgtacg ccaaagtgca gatcgtccga aaagtacggc
     4501 ttgtgcggta agcggtcgtt gaagagtgcg agtgcggcgt tatgcaatgt gccctgcgct
     4561 tatggcgggc tgttgtctgg cacggttcat gtgctaatat cctttcaagg tttcgacctg
     4621 gccctggtag tcatgctcat ccgccaagat agctcatgat tgccggggct ttgttttttc
     4681 tgttgccgtt aaattacacg cgaacggcct cgtctgtcga atccttctgt cacaacggct
     4741 tagtgcgccg ccagctcctg gaagaatttc gggttcgcct ggatggttgc cagcaccttc
     4801 gccgccagtc cggtcggatt acggcgtccg gtttcccagc tcttgatcgt gtcgaggctg
     4861 gttcccagta cttttgccat ctcgctctga gatacgttca gctgtgccct gatggctttc
     4921 acgtctgcca gctcgtaacg ggtgacgcgg gcaggctctc tgacgccgtt tttgatttca
     4981 acggcttctt ccagcgatgc tttcagctcg tcaaaaaaac tcatgtcaca cctcgtcttt
     5041 cagttgttgg gtcagcgact tcagcacggc tttctccgcg tccgtcaggc tgtctttggt
     5101 gcttttcggg taggccatca ccagataaat cacctcggcg gtggccagaa agtaaataac
     5161 cctcgcactg ccgctttttc cctggttgcc cgtcgccatt ctgattttgc gcagcccacc
     5221 ggttttctgg atgagatcgc ctttatccgg tgactcaatc agcgtctttt gcagctccct
     5281 gagttcttca tccgtggcga tctgttttat ctgacgggta aacatcgggg tctcgataaa
     5341 ctcaatcgcg tgactcacgg ctgtctcctg tgttcaatag cgtacatagt acacttaatt
     5401 ttaatttcag aaaagcggtc acatcgaggg gccgtaatag cgggtcggct ttttctgcgg
     5461 ctccggctgc tgctcgcgca gcttctccgc acgggcgcgg tcgtgctgtg caatcagcgc
     5521 atcaagcggc tgacgttcct gcttaagctc tcgacctgca cgcgcaagct gtccacctga
     5581 ctgctcaagt gctgacacac cctgtcgctg ttcgtcagcg cctgtga
//
