LOCUS       CP052482                4654 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0050 plasmid pC16KP0050-6,
            complete sequence.
ACCESSION   CP052482
VERSION     CP052482.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609883
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:57:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,637
            Genes (coding)                    :: 5,505
            CDSs (with protein)               :: 5,505
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 69 of 132
            Pseudo Genes (incomplete)         :: 66 of 132
            Pseudo Genes (internal stop)      :: 18 of 132
            Pseudo Genes (multiple problems)  :: 20 of 132
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0050-6"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4624..4654,1..536))
                     /locus_tag="HJW95_28750"
     CDS             complement(join(4624..4654,1..536))
                     /locus_tag="HJW95_28750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL75435.1"
                     /translation="MNMKTLKDIIEKQKKEEEEQKFDPAEQIEEYISLVEKLYTITLES
                     IDELIKDGLIKQSRKKISINEESLGDYEIDSLILTINSSKIKFEPVGTMLIGSKGRVDV
                     TGPFGKEKFILIRKGVKSPSDLIKVRVNVVGAYKPEKDKTDEDVKKPSIDDWEWKTMPS
                     DSRWVKFDDVNSETITNIIMRMING"
                     /name="hypothetical protein"
     gene            complement(803..916)
                     /locus_tag="HJW95_28755"
     CDS             complement(803..916)
                     /locus_tag="HJW95_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJL75429.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(941..1375)
                     /locus_tag="HJW95_28760"
                     /pseudo=""
     CDS             complement(941..1375)
                     /locus_tag="HJW95_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            1332..1523
                     /locus_tag="HJW95_28765"
     CDS             1332..1523
                     /locus_tag="HJW95_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL75430.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1523..1924
                     /locus_tag="HJW95_28770"
     CDS             1523..1924
                     /locus_tag="HJW95_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL75431.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMLDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2708..2783)
                     /locus_tag="HJW95_28775"
     tRNA            complement(2708..2783)
                     /locus_tag="HJW95_28775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2748..2750),aa:Arg,seq:tct)
                     /name="tRNA-Arg"
     gene            complement(2964..3206)
                     /locus_tag="HJW95_28780"
     CDS             complement(2964..3206)
                     /locus_tag="HJW95_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL75432.1"
                     /translation="MSKESELEFRTKELERQMKGVQRRIEVVNAKYDSQTKKQERRIRD
                     LEIKTAVQSGVTQREVANIYELSPGRVNQIIKKVG"
                     /name="hypothetical protein"
     gene            3596..3991
                     /locus_tag="HJW95_28785"
     CDS             3596..3991
                     /locus_tag="HJW95_28785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL75433.1"
                     /translation="MNANEVKDILEHSNHDDWIVDDETGSFTYKNDLNLHIKRADYDNY
                     NSFSEPWAVSHPNPNACSVQYTVNYGSSFVDRKTLVSVDGHRATLPMPASANDLTVTSA
                     DVNFAKIVDTGERVDEYILRSGLKVQP"
                     /name="hypothetical protein"
     gene            complement(4080..4631)
                     /locus_tag="HJW95_28790"
     CDS             complement(4080..4631)
                     /locus_tag="HJW95_28790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL75434.1"
                     /translation="MARVHAIKRSGLHIDIDYVVHKKIALDRAVILRYSTKNPDYHADF
                     VGLSEPEIEREKDLILKENDTTHCLLLLTAIEAIFRLDADQKYCNKKRDPLSRKIKEIY
                     DKTKGEKIKLDDDLINTRMNEDDARLKKDYNDLKTCFKFRHWLAHGRYWHPKRIKNFYF
                     DSILIVIDSILNSTKYNFEV"
                     /name="hypothetical protein"
     Typing          complement(804..1211)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.866"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtaatggtct cggaattcac atcatcaaat ttaacccatc gactatcgct aggcattgtt
       61 ttccattccc aatcatcaat tgacggcttt ttcacatctt catcggtctt atctttttct
      121 ggtttatatg ccccgactac gttaactctt acttttatta aatcactggg gcttttgact
      181 ccttttctaa tcagaatgaa tttttctttt ccaaatggcc ctgtgacgtc aacccttcct
      241 tttgaaccta ttagcatggt tcctactggc tcaaatttta ttttacttga gtttatagtg
      301 agtattagag agtcgatttc ataatcacct agagactctt catttattga tatttttttt
      361 cttgattgtt ttatcaatcc atctttgatt agttcgtcaa tagattctaa tgtaatagta
      421 tataattttt caacaaggct tatgtattct tctatttgtt ccgctgggtc gaatttttgt
      481 tcttcttctt cttttttttg tttttcaatt atatctttca gcgttttcat gttcattttt
      541 tgtaacctct agatcggaat tattttacga ttctagcctt tgctttggtg tcgagcaatg
      601 tgttgttctt gacgcttaac ttgctcggag gtattgtcgc ttctgaaacg aaaaaaccgc
      661 cttggcgggc ggttttttcg aaggttcata gagttggcgc tcgttgaacc gaggtaactg
      721 gcttggagga gcgcagtcac caaaacttgt tctttcagtt tagccttaac tggcgcataa
      781 cttcaagaca aactcctcta aatcagttac cagtggctgc tgccagtggc gcttttgcat
      841 gtctttccgg gttggactca agatgatagt taccggataa gacgcagcag tcggactgaa
      901 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
      961 ggcgtggaat gagataaacg cggccataac agcggaatga cactggtaaa ccgaaaggca
     1021 ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct ttaaagtcct
     1081 gtcgggtttc gccaccactg atttgagtgt cattctctgt gatgcttgtc aggggggcgg
     1141 agcctatgga aaaacggccg ccgtgcggca tttccttttc cccgtcacgc taccgctcgg
     1201 ttttctcgag ctcttgtacg catactcctg ccgctcgccg cagccgaacg gcaggagcgt
     1261 agcgactgag tgagcgagga agcggaaaag agaacatacc tacactgagc actgacgctc
     1321 tgaggcctct tatgtctaaa caacagaaaa cagcgctgaa catggctaaa ttcatccaga
     1381 atcagtctct gctcctgctg gagaaactta acgagcttga tctcgatgtc gaagccgacc
     1441 tttgtgagaa actccacgat gatgcggagc atcttttccg tacactgtcc tcgagactgg
     1501 atgcattaca ggatggtaac tgatggccag agtgtctatt agtgaggctg cccgactgac
     1561 gggtaagagt agaacaacac tgcacaggtt aattaaagca ggtgacctgt ccacctgtag
     1621 cggtgaacgt aatgcaaaaa tgttagatac ttcagagctt ttacgtgtat ttggtccctt
     1681 tgagcaaccg aagggtgaac accattccgg acaggtctct gaacagcgtg atacagcctc
     1741 tgctgaccag agtgaacagg tgattcgtca tctgaaacag gagattgaac acctgaacac
     1801 cttacttttg tccaaggatt cgcacattga tagcctgaaa caagccatgc tccttcttga
     1861 aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc
     1921 ctgaccccgg catgcttcag ttttcctctc cgggcatccc ggggagttcc tgaaccttcc
     1981 caaaaatcgc cagatctctg aacgctcctc accactaaaa atacccacac cccacgcaaa
     2041 attaacattt tgcggatttg tatttaatat cagtagttta agttggtgaa atttcgtttt
     2101 ttaaatttga tttatataga tccgactcta tgacgactcg tcatagagtc cccggcgagt
     2161 cgtcatagag tcggtaacga ctcaggacta taaaaacaat gagttaataa cgacccgact
     2221 ctagacccga ctcccagttt ggttgttttt tagacgtggc gactctgggt acgactctag
     2281 tgaagagtcg ccggtgaatc gtgtggtgag tcgttcggtg acttgccata acgtgattgc
     2341 cggccgtcga cgaaccggat tcctgaacgt gctgaaaacc ggttttccgg ctgcgccggc
     2401 aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tgggaagaac
     2461 gccggaaaca ggacttaccc tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg
     2521 catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt
     2581 tgttgcctgt tttaccggtt tcccgtcaga aacgccctga ggccgtttta gcggtgcgcg
     2641 taatgcgacg ttatggtaaa tgctgtggcc agaatagttg cctcccccct aaaagggggg
     2701 aggcttttgg tggagacaat gggatttgaa cccacaacca tcagattaga agtccgctgc
     2761 tctaccgttg aagctatatc cccatctgga cccaacaaag tcgggaccgt ctttgattta
     2821 acatcactgc atgctttttg tgaagttttt tatgtgttta ccggtcgcat cagaacaggg
     2881 tatgtaatga gaccttatgg taaaaagcac gtcagtttgt attgcgagaa aaaaccgcct
     2941 tttggcggtt ttttctctag agattagccg acttttttaa tgatctgatt aacgcgaccc
     3001 ggcgacagtt cgtaaatgtt tgcaacctca cgttgcgtaa cgcctgactg gactgctgtt
     3061 ttgatttcca aatcacgaat gcgacgttct tgcttcttgg tttggctgtc gtatttggcg
     3121 ttaaccacct caatacgtcg ctggacacct ttcatttgac gttcgagttc ttttgttctg
     3181 aactctagtt ctgattcttt actcatatga tatactcgct aagtaaggtt attgttgggt
     3241 gtcattcgaa ggtttcttct tgccggaagg tcttaaccta tgtttgacaa atcaacaata
     3301 gccttttttt acgatcacag caacttctat aatcagtgtt tccccttatt tttctttgtt
     3361 tttattttcc aataaaaaca ataagttgcg attggtttaa ttctgatatt aaaaaggcta
     3421 aacacccctg ttttatgtat atgattttta agtgaaaaaa caaatcgatg tttagtcttt
     3481 attatttgat tctttactgg aaaatttgat tgcacataga gaacttttcg gagcagtgtg
     3541 gtgtagaact caatcgatct ttacattcta accattgatt tatatggggg tatgcatgaa
     3601 tgctaatgaa gtaaaagaca ttcttgaaca ttctaatcat gatgattgga ttgtggatga
     3661 tgagactggg tcgtttacat ataaaaacga cttaaatttg cacattaaga gagcggacta
     3721 tgacaactac aactccttta gtgaaccctg ggctgttagt catccgaatc cgaatgcctg
     3781 ttcagtacag tacaccgtaa actatggttc atcatttgtt gatagaaaga ctttggtttc
     3841 tgtcgatggt caccgcgcaa ccttgcctat gcctgcatcc gcaaatgatt taaccgtgac
     3901 atcagctgac gtgaactttg ccaaaattgt agatactggg gagcgtgttg atgaatacat
     3961 tctccgctct ggtcttaaag ttcaaccatg atttaaatgt gagccagcaa tctaaaattg
     4021 ctggctcata atgtgctgtg cttaagggac gttatggtga cttttttaat gtcactgacc
     4081 taaacttcaa aattgtattt ggttgagttt aaaatggaat ctatcactat tagtatagag
     4141 tcgaaataaa aattctttat tctctttgga tgccagtatc ttccatgagc aagccaatgc
     4201 ctgaatttaa aacatgtttt tagatcgttg tagtcttttt ttaatctagc atcatcttca
     4261 ttcattctgg tatttattaa atcatcatct aattttattt tttctccttt tgttttatca
     4321 tatatctctt ttatttttct tgataatggg tctctttttt tattgcagta cttctgatct
     4381 gcatctaatc gaaatattgc ttcaatagct gttaacaata aaaggcaatg tgttgtgtca
     4441 ttttctttta gtattaagtc tttttctctt tcaatctccg gctcacttaa gccgacgaaa
     4501 tcggcatggt aatctgggtt ctttgttgaa taccgcagaa ttacagcacg atcgagtgct
     4561 atctttttat gtacgacata gtcaatgtct atgtgtagac cagatctttt aattgcatga
     4621 accctagcca tttatcattc tcataataat attc
//
