LOCUS       CP052475                4654 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0053 plasmid pC16KP0053-7,
            complete sequence.
ACCESSION   CP052475
VERSION     CP052475.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609884
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:50:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,901
            CDSs (total)                      :: 5,775
            Genes (coding)                    :: 5,620
            CDSs (with protein)               :: 5,620
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 78 of 155
            Pseudo Genes (incomplete)         :: 84 of 155
            Pseudo Genes (internal stop)      :: 22 of 155
            Pseudo Genes (multiple problems)  :: 28 of 155
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0053"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0053-7"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(16..411)
                     /locus_tag="HJW96_29465"
     CDS             complement(16..411)
                     /locus_tag="HJW96_29465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54035.1"
                     /translation="MNANEVKDILEHSNHDDWIVDDETGSFTYKNDLNLHIKRADYDNY
                     NSFSEPWAVSHPNPNACSVQYTVNYGSSFVDRKTLVSVDGHRATLPMPASANDLTVTSA
                     DVNFAKIVDTGERVDEYILRSGLKVQP"
                     /name="hypothetical protein"
     gene            801..1043
                     /locus_tag="HJW96_29470"
     CDS             801..1043
                     /locus_tag="HJW96_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54036.1"
                     /translation="MSKESELEFRTKELERQMKGVQRRIEVVNAKYDSQTKKQERRIRD
                     LEIKTAVQSGVTQREVANIYELSPGRVNQIIKKVG"
                     /name="hypothetical protein"
     gene            1224..1299
                     /locus_tag="HJW96_29475"
     tRNA            1224..1299
                     /locus_tag="HJW96_29475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1257..1259,aa:Arg,seq:tct)
                     /name="tRNA-Arg"
     gene            complement(2083..2484)
                     /locus_tag="HJW96_29480"
     CDS             complement(2083..2484)
                     /locus_tag="HJW96_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL54037.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMLDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2484..2675)
                     /locus_tag="HJW96_29485"
     CDS             complement(2484..2675)
                     /locus_tag="HJW96_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL54038.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2632..3066
                     /locus_tag="HJW96_29490"
                     /pseudo=""
     CDS             2632..3066
                     /locus_tag="HJW96_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            3091..3204
                     /locus_tag="HJW96_29495"
     CDS             3091..3204
                     /locus_tag="HJW96_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJL54039.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            3471..4037
                     /locus_tag="HJW96_29500"
     CDS             3471..4037
                     /locus_tag="HJW96_29500"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54040.1"
                     /translation="MNMKTLKDIIEKQKKEEEEQKFDPAEQIEEYISLVEKLYTITLES
                     IDELIKDGLIKQSRKKISINEESLGDYEIDSLILTINSSKIKFEPVGTMLIGSKGRVDV
                     TGPFGKEKFILIRKGVKSPSDLIKVRVNVVGAYKPEKDKTDEDVKKPSIDDWEWKTMPS
                     DSRWVKFDDVNSETITNIIMRMING"
                     /name="hypothetical protein"
     gene            4030..4581
                     /locus_tag="HJW96_29505"
     CDS             4030..4581
                     /locus_tag="HJW96_29505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54041.1"
                     /translation="MARVHAIKRSGLHIDIDYVVHKKIALDRAVILRYSTKNPDYHADF
                     VGLSEPEIEREKDLILKENDTTHCLLLLTAIEAIFRLDADQKYCNKKRDPLSRKIKEIY
                     DKTKGEKIKLDDDLINTRMNEDDARLKKDYNDLKTCFKFRHWLAHGRYWHPKRIKNFYF
                     DSILIVIDSILNSTKYNFEV"
                     /name="hypothetical protein"
     Typing          2797..3204
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.866"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tggctcacat ttaaatcatg gttgaacttt aagaccagag cggagaatgt attcatcaac
       61 acgctcccca gtatctacaa ttttggcaaa gttcacgtca gctgatgtca cggttaaatc
      121 atttgcggat gcaggcatag gcaaggttgc gcggtgacca tcgacagaaa ccaaagtctt
      181 tctatcaaca aatgatgaac catagtttac ggtgtactgt actgaacagg cattcggatt
      241 cggatgacta acagcccagg gttcactaaa ggagttgtag ttgtcatagt ccgctctctt
      301 aatgtgcaaa tttaagtcgt ttttatatgt aaacgaccca gtctcatcat ccacaatcca
      361 atcatcatga ttagaatgtt caagaatgtc ttttacttca ttagcattca tgcatacccc
      421 catataaatc aatggttaga atgtaaagat cgattgagtt ctacaccaca ctgctccgaa
      481 aagttctcta tgtgcaatca aattttccag taaagaatca aataataaag actaaacatc
      541 gatttgtttt ttcacttaaa aatcatatac ataaaacagg ggtgtttagc ctttttaata
      601 tcagaattaa accaatcgca acttattgtt tttattggaa aataaaaaca aagaaaaata
      661 aggggaaaca ctgattatag aagttgctgt gatcgtaaaa aaaggctatt gttgatttgt
      721 caaacatagg ttaagacctt ccggcaagaa gaaaccttcg aatgacaccc aacaataacc
      781 ttacttagcg agtatatcat atgagtaaag aatcagaact agagttcaga acaaaagaac
      841 tcgaacgtca aatgaaaggt gtccagcgac gtattgaggt ggttaacgcc aaatacgaca
      901 gccaaaccaa gaagcaagaa cgtcgcattc gtgatttgga aatcaaaaca gcagtccagt
      961 caggcgttac gcaacgtgag gttgcaaaca tttacgaact gtcgccgggt cgcgttaatc
     1021 agatcattaa aaaagtcggc taatctctag agaaaaaacc gccaaaaggc ggttttttct
     1081 cgcaatacaa actgacgtgc tttttaccat aaggtctcat tacataccct gttctgatgc
     1141 gaccggtaaa cacataaaaa acttcacaaa aagcatgcag tgatgttaaa tcaaagacgg
     1201 tcccgacttt gttgggtcca gatggggata tagcttcaac ggtagagcag cggacttcta
     1261 atctgatggt tgtgggttca aatcccattg tctccaccaa aagcctcccc ccttttaggg
     1321 gggaggcaac tattctggcc acagcattta ccataacgtc gcattacgcg caccgctaaa
     1381 acggcctcag ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac
     1441 ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg agtatgcacg caagtgcata
     1501 gcgcgaaaat tcacgccatt cagccagggt aagtcctgtt tccggcgttc ttcccatccg
     1561 aaaacggtga acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg
     1621 aaaaccggtt ttcagcacgt tcaggaatcc ggttcgtcga cggccggcaa tcacgttatg
     1681 gcaagtcacc gaacgactca ccacacgatt caccggcgac tcttcactag agtcgtaccc
     1741 agagtcgcca cgtctaaaaa acaaccaaac tgggagtcgg gtctagagtc gggtcgttat
     1801 taactcattg tttttatagt cctgagtcgt taccgactct atgacgactc gccggggact
     1861 ctatgacgag tcgtcataga gtcggatcta tataaatcaa atttaaaaaa cgaaatttca
     1921 ccaacttaaa ctactgatat taaatacaaa tccgcaaaat gttaattttg cgtggggtgt
     1981 gggtattttt agtggtgagg agcgttcaga gatctggcga tttttgggaa ggttcaggaa
     2041 ctccccggga tgcccggaga ggaaaactga agcatgccgg ggtcaggatt ttttccagaa
     2101 tttccaccat ggcgccgggg attcagttaa ttttgcctgg tgattttcaa gaaggagcat
     2161 ggcttgtttc aggctatcaa tgtgcgaatc cttggacaaa agtaaggtgt tcaggtgttc
     2221 aatctcctgt ttcagatgac gaatcacctg ttcactctgg tcagcagagg ctgtatcacg
     2281 ctgttcagag acctgtccgg aatggtgttc acccttcggt tgctcaaagg gaccaaatac
     2341 acgtaaaagc tctgaagtat ctaacatttt tgcattacgt tcaccgctac aggtggacag
     2401 gtcacctgct ttaattaacc tgtgcagtgt tgttctactc ttacccgtca gtcgggcagc
     2461 ctcactaata gacactctgg ccatcagtta ccatcctgta atgcatccag tctcgaggac
     2521 agtgtacgga aaagatgctc cgcatcatcg tggagtttct cacaaaggtc ggcttcgaca
     2581 tcgagatcaa gctcgttaag tttctccagc aggagcagag actgattctg gatgaattta
     2641 gccatgttca gcgctgtttt ctgttgttta gacataagag gcctcagagc gtcagtgctc
     2701 agtgtaggta tgttctcttt tccgcttcct cgctcactca gtcgctacgc tcctgccgtt
     2761 cggctgcggc gagcggcagg agtatgcgta caagagctcg agaaaaccga gcggtagcgt
     2821 gacggggaaa aggaaatgcc gcacggcggc cgtttttcca taggctccgc ccccctgaca
     2881 agcatcacag agaatgacac tcaaatcagt ggtggcgaaa cccgacagga ctttaaagat
     2941 accaggcgtt tccccctgat ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     3001 ccagtgtcat tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcagttcc
     3061 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc
     3121 tgcgtcttat ccggtaacta tcatcttgag tccaacccgg aaagacatgc aaaagcgcca
     3181 ctggcagcag ccactggtaa ctgatttaga ggagtttgtc ttgaagttat gcgccagtta
     3241 aggctaaact gaaagaacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     3301 caacgagcgc caactctatg aaccttcgaa aaaaccgccc gccaaggcgg ttttttcgtt
     3361 tcagaagcga caatacctcc gagcaagtta agcgtcaaga acaacacatt gctcgacacc
     3421 aaagcaaagg ctagaatcgt aaaataattc cgatctagag gttacaaaaa atgaacatga
     3481 aaacgctgaa agatataatt gaaaaacaaa aaaaagaaga agaagaacaa aaattcgacc
     3541 cagcggaaca aatagaagaa tacataagcc ttgttgaaaa attatatact attacattag
     3601 aatctattga cgaactaatc aaagatggat tgataaaaca atcaagaaaa aaaatatcaa
     3661 taaatgaaga gtctctaggt gattatgaaa tcgactctct aatactcact ataaactcaa
     3721 gtaaaataaa atttgagcca gtaggaacca tgctaatagg ttcaaaagga agggttgacg
     3781 tcacagggcc atttggaaaa gaaaaattca ttctgattag aaaaggagtc aaaagcccca
     3841 gtgatttaat aaaagtaaga gttaacgtag tcggggcata taaaccagaa aaagataaga
     3901 ccgatgaaga tgtgaaaaag ccgtcaattg atgattggga atggaaaaca atgcctagcg
     3961 atagtcgatg ggttaaattt gatgatgtga attccgagac cattacgaat attattatga
     4021 gaatgataaa tggctagggt tcatgcaatt aaaagatctg gtctacacat agacattgac
     4081 tatgtcgtac ataaaaagat agcactcgat cgtgctgtaa ttctgcggta ttcaacaaag
     4141 aacccagatt accatgccga tttcgtcggc ttaagtgagc cggagattga aagagaaaaa
     4201 gacttaatac taaaagaaaa tgacacaaca cattgccttt tattgttaac agctattgaa
     4261 gcaatatttc gattagatgc agatcagaag tactgcaata aaaaaagaga cccattatca
     4321 agaaaaataa aagagatata tgataaaaca aaaggagaaa aaataaaatt agatgatgat
     4381 ttaataaata ccagaatgaa tgaagatgat gctagattaa aaaaagacta caacgatcta
     4441 aaaacatgtt ttaaattcag gcattggctt gctcatggaa gatactggca tccaaagaga
     4501 ataaagaatt tttatttcga ctctatacta atagtgatag attccatttt aaactcaacc
     4561 aaatacaatt ttgaagttta ggtcagtgac attaaaaaag tcaccataac gtcccttaag
     4621 cacagcacat tatgagccag caattttaga ttgc
//
