LOCUS       CP052474                5044 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0053 plasmid pC16KP0053-6,
            complete sequence.
ACCESSION   CP052474
VERSION     CP052474.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609884
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:50:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,901
            CDSs (total)                      :: 5,775
            Genes (coding)                    :: 5,620
            CDSs (with protein)               :: 5,620
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 78 of 155
            Pseudo Genes (incomplete)         :: 84 of 155
            Pseudo Genes (internal stop)      :: 22 of 155
            Pseudo Genes (multiple problems)  :: 28 of 155
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0053"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0053-6"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(5026..5044,1..404)
                     /locus_tag="HJW96_29430"
     CDS             join(5026..5044,1..404)
                     /locus_tag="HJW96_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL54028.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(750..992)
                     /locus_tag="HJW96_29435"
     CDS             complement(750..992)
                     /locus_tag="HJW96_29435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54029.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1148..1471)
                     /locus_tag="HJW96_29440"
     CDS             complement(1148..1471)
                     /locus_tag="HJW96_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54030.1"
                     /translation="MKKLVAIGLGVLILSGCATQKQMTPMGGSKADGTVKMGYTFGMFE
                     KPVVDLNSAKDLAGQKCKTWGYTGAEAFGGQTSTCAQVGAYGCEMTNVSIEYQCTGGKA
                     SEN"
                     /name="hypothetical protein"
     gene            complement(3247..3552)
                     /locus_tag="HJW96_29445"
     CDS             complement(3247..3552)
                     /locus_tag="HJW96_29445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54031.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGDLSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(3609..3986)
                     /locus_tag="HJW96_29450"
     CDS             complement(3609..3986)
                     /locus_tag="HJW96_29450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54032.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            complement(4444..4878)
                     /locus_tag="HJW96_29455"
     CDS             complement(4444..4878)
                     /locus_tag="HJW96_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="QJL54033.1"
                     /translation="MNLAMFSAVFCCFVIKGLRASVLSVKPVSFPLPRSLSRYAPVVRL
                     RRAAGACIEKLEKTERSKEEKKERPRRCRFSIGSAPLTSIAETDAQISGGETRQDYKDT
                     RRFPLAAPSCALLFLPFGLPVSLRCYGRVYLIPRLTLSSG"
                     /name="plasmid replication initiation protein"
     gene            4835..5026
                     /locus_tag="HJW96_29460"
     CDS             4835..5026
                     /locus_tag="HJW96_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL54034.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2108..2323
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.284"
                     /coverage="100.0"
                     /evalue="7.22e-78"
     Typing          complement(4312..4677)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 gtgaagctgc acggcttgtt aaggttagtc gtcccactat ttacaagatg attaacagcg
       61 ggaagttaag ttatacctct gttgtaaagc acgggaagga tattaaagtt attgatgtat
      121 ccgagcttat tagggtgttt ggttctcttg atggtgtaac tgatactgta aaatatgatg
      181 taaaaagtga cgcagaatct acaggtatta acagcgttgg tttacatgat ttacaacata
      241 gaatcgcttt acttgaagct gaaaacgatg gtcttaaagg ggctgttaag gctcgagatg
      301 agcatattga ttcccttcgt caggctatgc agcttctgga gcacaagcac gaaccttctt
      361 caccatccca ttccccgtgg tggaaattct ggaaaaaatc ctgatcctgc catgcttgag
      421 ttttcctctc cggccatccc ggagagtttc tgaaccttcc caaaaatcgc cagatctctg
      481 aacgctcctc accactaaaa atacccacac cccacgcaaa attaaaattt tgcagattca
      541 ttatttacaa caatgggtta atgaaaaata aatgtgcggt gatgtgttac aaagtgcaga
      601 tccaagtcgt ggacgactct atggcgaatc gctgacgaat tgcccacgat tcatatgtga
      661 atcacgcttt attatcatat tgttatgatg aatcgactct tgattcaatt catcactttg
      721 ggtgtttttt gctcatggtg actcttgatt caattcacga ttcgaatcgc cggtgaatcg
      781 tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcgac gaacccgatt
      841 cctgaaggtg ctgaaaaccg gttttccggc tgcgccggca acgcttcacg gccacgcaat
      901 aaattgcgtc cccgttcacc gttttcggag gagaagaacg ccggaaacag gacttcccct
      961 ggctgaatgg tgtgaatttc cgcgctatgc acttgcgcgc atactcatgc atgccgtaaa
     1021 aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt ttaccggttt
     1081 cccgtcagaa acgccctcag agcctctcag gcggtgcacg caagggatgt tatggtaaat
     1141 gttgaactta gttttctgaa gctttcccac cggtgcattg gtactcgatt gaaacgttgg
     1201 tcatctcaca accataagcc ccgacctgtg cgcaggtgga tgtttggccg ccaaatgcct
     1261 cagccccagt gtatccccat gttttacatt tttgtccagc cagatcttta gctgagttca
     1321 gatcaacaac gggtttttca aacatcccaa aggtgtaacc cattttcact gtaccatcag
     1381 ctttactccc ccccatcggg gtcatttgtt tttgcgttgc gcagcctgaa agaattaaaa
     1441 cgcctaaacc gatcgctact agttttttca ttttttattt cccggatgat tgttcggaat
     1501 tatcttaacg ggaagtttac acaaggcaac accctctgta atcctctgat tatttgaggt
     1561 atcccataaa aaaatcaaag aaagcctaat gcccccgtgg tgggtatata aaaatacggg
     1621 gagcggagcg accttcgtct agctcttgtt ttgtttttct tgccgcgcaa ctggcttgat
     1681 tgtgtgggtg ttgtgtccga cgtctctcac atcaccgcgt actgctgcct ttttcgctca
     1741 ttttatcagg tgcaagttat cgtactgcga gacggggtag agcctaactc tttcgcggtt
     1801 aagttacgtt cccaccaatt tgcctgttta tcctgccgga gtccggggcg tagacctgag
     1861 cgtctaaggt gcagatatga ttttaatgtc gttgaatggt attaagctgg tagtgacatg
     1921 gctagccagc cctgctaaga tttgattgcg acgtctaatc cagcaggtat ctgcacgaat
     1981 tagagtaccg aagccctgtg attatgtcac ggggcttttg tacatttagc gcccgagaat
     2041 caaaaacatc ccttcagaac ttatgtaacg cccgccgcag tctcacggca ggagcgcagc
     2101 gaccaagtga gcgaggaggc gacatttcat ccttacctgc atgttgcact tacgccccgc
     2161 agctacattt ttcccctgcc acatgaagca cttccctgaa ttcccacttc gctttaccat
     2221 attaagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac
     2281 ccgctaacaa cctctctgct actggatatc accccatgcc atctgaatat gatgaacaac
     2341 ggggttcgac tgatacgcag cttcccggcg aatatgacct ttttgattta atggcgaagg
     2401 ttactgaaga gatcctgcat gcccctgtgg attctgggcc gcctgtcggc cgggaagcgt
     2461 tatagctttt tcgcttgccc gcgtacgtgg taatacgccg gtattacctt ttatttcttt
     2521 attttcatgt aattacagtg ggtttttaat acctgggtat tactcgtgaa aacgaaacat
     2581 tacttttttt tcttttaatt catgcagttg cgatgaaaat cgcggctgcg ttaggtgtat
     2641 gaccattttg aggttgaaaa ataatcaacc atggcgggtg atttacgcag gttaagggga
     2701 gatcgtgaca aaaaaaagcg ttcctcactg tatcgttcat gcctgcatga acgggttatt
     2761 cttcctgact acagaattac atcgctcctt cagttcatac atcactttta caacactttt
     2821 acaacactcc ttcggcctgt acatcacttt tacaacactt ttgcatcact tttacatcat
     2881 ttatacatca cttttacatc attccttcag ctcatacgtc actttcatcc tctgtgccat
     2941 ttgtacatca cccccttcct gaacgtgctg acagcgggtt ttccggctgc gccggcaacg
     3001 cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt ttcggatggg aagaacgccg
     3061 gaaacaggac ttcccctggc tgaatggtgt gaatttccgc gctatgcact tgcgcgcata
     3121 ctcatgcatg ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg gtggtttgtt
     3181 gcctgtttta ccggtttccc gtcagaaacg ccctgagggc ctctcaggcg gtgctgataa
     3241 cggatgttat ggtaaatgtt ggatagcaag gactatcagt ccggcaatgc tacatatcat
     3301 tgctaatgga tttacgagca aaatgccgaa tgttgtcgca gataatccgc gaacaatgat
     3361 attggcaaga agtccgccaa caatgaggga taaaaaggta taaccagcaa ctttcagtaa
     3421 cgacagatct ccccatatca cctctactat cggatagata aaaacaccaa cgccagcagg
     3481 aagcgtagta accatcaaca gtaaatggct tttagtggga ccgtactctc tgacatttcc
     3541 aactttcatc atgtgtgtga tgaatgacga tatccaccat aggatcatga tcataaggaa
     3601 attcataatt atttactctc gatgcgtaga tcaggtgctt tctgtagatt tggtcgtaca
     3661 tactcgctgt ctctttcgtc aagacaacgg aagtagagtg ttactgtggt atctatcccc
     3721 attcttgtgt tagccacgtt ttcgttagtg agcaggaatt ttttaccgag gctattacat
     3781 ttctcagaac ctgttttaaa ggcactttga cgtgctgatg ccgcatctct aaaaccatca
     3841 gcagttgctg aaaccgtata tgtatcccta cctaactcaa gaacgttgct ggtagaaaca
     3901 caaccaactg taagcagcga tattgataaa atacctaacc tataaatcat tgattttatt
     3961 acccaagttg ggtgattgtg gatcatgatt atatctgttt tttttattat taaaatcaag
     4021 gggttcaata attttcttca aagaactggt gtcgtggagt ggtgctggtt tccctttggg
     4081 ggggagaggg atggaatagg atcctcttga gatcctttct gttgcgtcgt aatctcttgc
     4141 cctggaaacg aaaaaaccgc ccagcaaggc ggttttttcg aaggttctta gagcagcaac
     4201 tctttgaacc gaggtaactg gctttcgcag agcacagaaa ccaaattagt tattacagtg
     4261 tagctgtact tcacctcaca cttcaacact tctttgttct gcgaatccgt taccagtggc
     4321 tgctgccagt ggcgataagt cgtgtctttc cggattggac tcaagacgat agttaccgga
     4381 taaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     4441 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggag
     4501 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa
     4561 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtccgtttc
     4621 tgcgatgctt gtcagggggg cggagcctat ggaaaaacgg caacgccgtg gcctttcctt
     4681 tttttcctcc tttgaccgct cggttttctc gagcttttct atgcatgctc ctgccgctcg
     4741 ccgcagccga acgaccggag cgtagcgact gagtgagcga ggaagcggaa aagagacagg
     4801 ttttacactg agcactgacg ctctgaggcc ctttatgacg aaacaacaga aaacggcgct
     4861 gaacatggct aaattcatcc agaatcagtc tctgctcctg ctggagaagc ttaacgagct
     4921 tgatcttgat gctgaggccg acctttgtga gaaactccac gatgatgcag agcatctttt
     4981 ccgtacactc tcctcgagac tggattcatt acaggatggt aactgatggc cagagtgtct
     5041 atta
//
