LOCUS       CP052473               10071 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0053 plasmid pC16KP0053-5,
            complete sequence.
ACCESSION   CP052473
VERSION     CP052473.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609884
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:50:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,901
            CDSs (total)                      :: 5,775
            Genes (coding)                    :: 5,620
            CDSs (with protein)               :: 5,620
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 78 of 155
            Pseudo Genes (incomplete)         :: 84 of 155
            Pseudo Genes (internal stop)      :: 22 of 155
            Pseudo Genes (multiple problems)  :: 28 of 155
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0053"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0053-5"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(9006..10071,1..899)
                     /locus_tag="HJW96_29365"
     CDS             join(9006..10071,1..899)
                     /locus_tag="HJW96_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJL54018.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            899..1630
                     /locus_tag="HJW96_29370"
     CDS             899..1630
                     /locus_tag="HJW96_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJL54019.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            1637..2167
                     /locus_tag="HJW96_29375"
     CDS             1637..2167
                     /locus_tag="HJW96_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL54020.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2195..2374)
                     /locus_tag="HJW96_29380"
     CDS             complement(2195..2374)
                     /locus_tag="HJW96_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL54021.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2443..2820
                     /locus_tag="HJW96_29385"
     CDS             2443..2820
                     /locus_tag="HJW96_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJL54022.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(2882..3181)
                     /locus_tag="HJW96_29390"
     CDS             complement(2882..3181)
                     /locus_tag="HJW96_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJL54023.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3171..3434)
                     /locus_tag="HJW96_29395"
     CDS             complement(3171..3434)
                     /locus_tag="HJW96_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJL54024.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(3740..4159)
                     /gene="exc2"
                     /locus_tag="HJW96_29400"
     CDS             complement(3740..4159)
                     /gene="exc2"
                     /locus_tag="HJW96_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJL54025.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            4562..5641
                     /locus_tag="HJW96_29405"
                     /pseudo=""
     CDS             4562..5641
                     /locus_tag="HJW96_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /name="cloacin"
     gene            5696..6393
                     /locus_tag="HJW96_29410"
                     /pseudo=""
     CDS             5696..6393
                     /locus_tag="HJW96_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            6410..7024
                     /locus_tag="HJW96_29415"
                     /pseudo=""
     CDS             6410..7024
                     /locus_tag="HJW96_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152554.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            7034..7291
                     /locus_tag="HJW96_29420"
     CDS             7034..7291
                     /locus_tag="HJW96_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJL54026.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            7376..7525
                     /locus_tag="HJW96_29425"
     CDS             7376..7525
                     /locus_tag="HJW96_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJL54027.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..10071
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7629..8207)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          900..1627
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gtttccgcag ggggcaaaca
       61 ttcagcaggt gctggcggcg gcgtacgcgc tgccggaggc cctgaaaaag gacgtgaagg
      121 ggttgatcac taggctggaa aacgtcgctg acctgagcgt cctgcagacg gacagtggca
      181 tcgatgtggc ggggattatc aacggcggcg gctgcctgta cgttatcggc tcgatggacg
      241 acgaggcggt gatccgtgtg caaaaaatgc tgtttgcccg ctgcgcgcaa atcatcattg
      301 cgcgggacga gttccgggag tggccacacg tcagcgtgat gctcgatgaa attaaatatc
      361 tgctgtcgaa gtatgtactg aacgcactgg ggacgctgcg cagccgggac tgcaatctgt
      421 tgctcgcgca ccagtcgctg ggcgatttcg ggcagtgcgg gcaggatctg cccgctgaat
      481 ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttttaccgg gcggccagcc
      541 tggagtccgc gcagtgggcg gcggggcaga ccggggaaat cagggtggac gtggagcgcc
      601 gccgtgccag ccgggaggcg gggaacgtgg agcatatcag cggggacagc ttcatccaga
      661 aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca cctgccggac gggttcgcgg
      721 tcatgaccgg gctgggtgtg gcccggatgg cgttcagcag tccgctgcgg gttgagcgcc
      781 gggaaatccc gttgcagcgt ttcccggtgc tggcgaaaac cgatcccctg gcggagttcc
      841 ggcccccggc cgacggcggc gggccgaaag cggatgatga ttttgcgggg atttactgat
      901 ggcgctggag ctgtacaacg tcagccacgc gaaacgacag gcgcggaacg cggagaaaac
      961 ccggctgacc ctgcgctggt tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg
     1021 gcggctgggg attgcggcgg tgcagccggt ctaccgcttc ctcgacagcc tggtggcgaa
     1081 agggctgctg gtccggacca aatatccggt ggacggccgt caggtcagcg tgtgggggct
     1141 gacgccgcat ggcgtggcgt tctcgttcga cgaggacgaa ccgctggcgg atgttattcc
     1201 gtttcagccg tcaagggtgt cggcagcaca tctgccgcac cggctggccg tgcaatccct
     1261 gcgccttgat atggaagcca gaggggccac cggctggcgc attctgcacc gtctgagtct
     1321 taaaggcatg aaagtgccgg acgccctggc ggagctggac ggtaaaacgg tggcgtttga
     1381 ggtggagcgc accgtcaaga gccgtcgccg gtatcaggaa gtggtgtccg ggtatctgtt
     1441 caaccgcaaa gctaacggca tcgatgaaat ctggtacatc tgcccggacc gggcgacgca
     1501 ggtcagggtg cagcgggcaa tcctgtcggt ggatgaaatc gtgaacccac agaccggcga
     1561 ggcccgcaaa acggcagagc tggatcgtga acggttgttt gcgtgcttta aatttatgac
     1621 aacggagtga gggattatgg cgctgtacat ggtatttatt gaagacgcgg aggaccggaa
     1681 cggcgagcgc tgcatcatgc cgaccagcga gcatctggaa gatatggtgg tgctcgcctg
     1741 gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa
     1801 atccatcggg atgtatgacg agcggctcaa cagcgacgaa aaagccacgt actttttcac
     1861 tggtaaaaat ccgcaatggg aacggaacaa acccacagga atgaagctgt gtgattacct
     1921 ctatcgcaag gtggagcggt tcgccatcga gaacggtgtg caggtggttg atgccgggaa
     1981 gtgctcaccg gagcagatta acgcgatgat tgacgtggtg agtgagcggc acggggaaag
     2041 cgcggtgaac gagaagctga aagcggagct gatgcgggaa tataacagcc gctattttaa
     2101 cgtgccggta cctgaactgc cggaggacaa acctgcccgc cggtggtggc cgttcggcaa
     2161 aaaataaatg ccagcaccgg ccatgccggt gctgttattc ctccccgagc ttatcccgca
     2221 tcacctgcca gagtgtttcc gcatcctcgt gtagccgctc gcacatgtcg gccggggtat
     2281 ccatatccag ctcattcagt ttatcgagca atagcagcga ctgctcctta ataaatttag
     2341 ccatattgag ggctgttttt tcctgttttg tcatgattct ttccttccgg gcatggtgtg
     2401 cgcctgtcag gtatactgtc tcaaaaatag cggggtgaga caatggcgtg ggtgacggtg
     2461 agacaggcga cggaactgac gggtaaagcc cgaagttccc tgtatcggga tatggcgaaa
     2521 ggccgcgtat cataccgtac ggaagctgat ggcggccggg tggtggatac cagcgagctt
     2581 atccgggtgt acggtgagtt aagacaggat gagactcacg agcgggacga gttgagactg
     2641 tcgggtgaga ctgaaaaaat ggcctccgac gcgttgatcg cggagataaa agcactcagg
     2701 gaggaggtgg ccgggctacg gcaggaaatg cagtccatga gactgctgga acacaaaccg
     2761 gagacagcag agaccccggc cggaacaccg cgccggtggt ggccgtgggg aaaacgctga
     2821 cgcgctgtgc ttgtccaacc ggcgacggcc acgcaatgaa ttgcgtcccc gtcaccggtt
     2881 attagagaac aatatgggga ttctggcgct ggccgaccac ggccagaacc cggacttttc
     2941 tggtctggtc atcaacatca tagaggacat gctgtcccat cagactgatc cggcgaacgt
     3001 cctcgccata gcgtgagtcc acttcataga gtcgcgggaa gtcggccagt ttgtccgccg
     3061 cagcgagaac ccggtcggta acgttctgcg catcccacag ggatgaattt tccgcgatat
     3121 agtcaaacag cgtcgttaca tcctcgatgg ctccctgctt ccattccacg tcatacggca
     3181 tcgcgcagtt cctgataacg gccatgcagg cgctggcgga gttgttccgt ggtcataccc
     3241 ggacgcgtgt cagcacgggc ggcggtcact ttggcgcgaa cctgatcttc atactctgcc
     3301 tgggccagtt cttcgttagc cagtcgctgc aacagcgcag ctttgtccgg gccaccttcc
     3361 ggcagtatgc ggggcaggct ttcagccagt tcccgaagaa agttggcgtt ttcgtagtgt
     3421 gtagtgttgc tcattgcgga gcctcccggg ttaatgcggc aatatgaagg aagtttagca
     3481 gggggcggtc tgaaagaata tggtcgtgtc agtcaggctg atgaagcaga aagtcggtaa
     3541 aaaagggctt acggcatcca ttttacgtca aaacatatgc catgcagttg cgtgcatagg
     3601 catgcattag gataaaattt atcgggcgcg tttccggcag ttttccgggg gggttgttgc
     3661 ctgttttatc ccgtagccgc cggaaacgcc ctgagcccgt ctgagcggtg cgcgtaatga
     3721 cgcgttatgg taaatccact tactgtctgc cttcatagcc atcgagataa acagctgaaa
     3781 tggtgtcagc cagtagcctt gattttttcg gtgtgaggtc tttgctgcgg taatcggtac
     3841 ccgcaaattg tgttgtgcct tggagtgagt ttaaaaattc ctgactgtga aactgctgaa
     3901 tgcgttgctg cacatcactc cgtgattttc cggtcgcttt atcctttgcg cccatatccc
     3961 agaactgccg gaaaaaaggc accatcattc gtgttgtgtc agctttctgg gtggaaaaat
     4021 tgggatcaaa accgtcatct gaagcatata cgtaatgccg ggtatgctgt tccacgctgc
     4081 cctttggagc acaggcagtg agagttgctg tcgctgttat catggcgata agacacagtt
     4141 tgctgccgga acgtctcatg agcgtctcct tacctttaat tcttttttct cattgagtta
     4201 tatatccgca ggctatctta tcatcagttt ctgaccgtgt tcagtgaccg aacaacccga
     4261 accagacccc ccgtttcttt tcctgtcccg gagggggctt tctggtatct catataatga
     4321 tttcagtaaa acatactgag gccggagaac ggcgccgttt gtgctggttt ttgttgcatc
     4381 tggaggggat tgcgcatggt gtgagtggct gtttttttga tcattttcac aaaaacgcaa
     4441 atttacagac ttgacatgaa aaacaaggca gcgtaatttt tatactgtat ataaaacaag
     4501 tggttatatg tacagtattt tattttagtt tattgtttta aaagtcaaag aggaaaagta
     4561 tatgagtggc ggagacggac gaggtccggg taattcaggt ctgggacaca atggtggtca
     4621 ggccagtggg aatgtgaacg gtacatcagg taaaggtggc ccttcatcag gtggtggtac
     4681 ggatccaaac agcgggccgg gctggggtac gacgcataca cctaacgggg atattcataa
     4741 ctataacccg ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gcggtaacag
     4801 cggcaatcat agcgggagct ctggtggcgg acagtcttcg gccaccgcga tggccttcgg
     4861 tctgcctgcc ttggccactc cgggggctga aggactggct ttatccgttt ccggcgatgc
     4921 gttgtcggcc gctgttgctg atgtgctggc tgccctgaaa ggtccgttta agtttggtct
     4981 gtgggggatt gcgatttacg gagtgctgcc ttctgagata gcaaaagatg atccgaaaat
     5041 gatgtcaaaa atcgtgacgt cattgccggc agacacggta acggagacgc cggtcagctc
     5101 cctgccgctg gaccaggcga cggtcagcgt cactaaacgt gtggcggata ttgtgaagga
     5161 cgagcggcag catattgcgg ttgtcaccgg ccggccaatg agtgttcctg tggtggatgc
     5221 gaaaccgaca aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca
     5281 ggtgagcgta cctaaaggtg ttccggccgc gaaagccccg ccaaaaggca ttattgctga
     5341 aaaaggtgat tcacgtccgg ctggttttac ggccggtggt aactcccgtg aagccgttat
     5401 tcgtttcccg aaagagagcg gacagaaacc ggtctatgtg tcggtgacgg atgttcttac
     5461 tccggcacag gtaaaacagc gtctggagga agaaaagcgt cgccagcagg catgggacgc
     5521 tgctcacccg gaagaggggc tgaaaagaga gtatgataaa gcgaaagctg agctggatgc
     5581 tgaagataaa aatattgcga ccttaaacag tcgcattgca tcgacagaga aggcgatccc
     5641 ggtgatgcta ccaacttgct gatttagtgt ataatggtgt ttttgaggtg ctcccgtggc
     5701 ttcagtctcc gtctgctgtc cctcctgttc cgctactgaa ggcgtgatgc gtaatggcaa
     5761 aagtactgcc gggcatcaac gttatctctg ctctcactgc cgtaaaacat ggcagctcac
     5821 cttcacttat gccgcttctc agcccggtac acaccagaaa atcattgata tggctatgaa
     5881 cggcgtcggt tgccgtgcca ccgcacgact aatgggcgtg ggcctcaaca ccattttgcg
     5941 ccatttaaaa aactcaggcc gcagtcagta acctcccgga tacagccggg cagtgatgtc
     6001 attgtctgtg cggagatgga cgagcaatgg ggctacgtcg gtgcaaaatc acgtcagcgc
     6061 tggctgtttt acgcgtatga caggatacgc agggcggtgg tagcccacgt tttcggtgaa
     6121 cgcactatgg ccacactgga gcgtcttctg agcctgctgt cagtttttga cgtggtggta
     6181 tggatgacgg acggctggcc gatgtatgag tcacgtctga agggaaaact gcacgtcatc
     6241 agtaagcgtt acacgcagcg aattgagcga cacaacctga atctgagaca gcatctggca
     6301 aggctgggca ggaagtccct gtcgttctca aaatcggtgg agctgcatga caaagtcatc
     6361 gggcactatc tgaacataaa acactatcag taaattggag tcattaccgg cgatccccgg
     6421 tgcaagggct gctgttcagg aagccgataa aaaggtaaaa gaggcagagg caaataaaga
     6481 tgattttgtg acttataacc cgcctcatga atatggctcc gggtggcagg atcaggttcg
     6541 ctatcttgat aaggatattc agaatcagaa tgagaaatta aaagctgccc agacatcttt
     6601 aaacgaaatg aatgaatcct tatccaggga taaggctgcg ctttccgggg cgatggagag
     6661 ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat aaattaaatg aggaaaagaa
     6721 aaaacctcgt aagggaacta aagattacgg ccatgattat tttcctgatc ccaagactga
     6781 agatattaag gggttgggag agttgaaaga gggtaaacct aaaaccccta aacaaggtgg
     6841 tggtggtaag cgggcccgat ggtatggtga taaaaagcgt aaaatttatg aatgggattc
     6901 ccagcacggt gagcttgaag ggtaccgcgc cagtgatggc gaacacctcg gggcattcga
     6961 tccaaaaacg ggtaagcagg ttaaagggcc ggatccaaaa cgaaacatta aaaaatatct
     7021 ttaagaggta aatatggggc ttaaattaca tattcattgg tttgataaga aaaccgaaga
     7081 gtttaaaggt ggtgaatact caaaagactt cggtgatgat ggttctgtca ttgaaagtct
     7141 ggggatgcct ttaaaggata atattaataa tggttggttt gatgttgaaa aatcatgggt
     7201 ttcgatatta cagccacact ttaaaaatgt aatcgatatt agtaaatttg attactttgt
     7261 atcctttgtt taccgggatg gtaactggta agcaaaagtt atcaaggatg atttgaaata
     7321 cagacttccg gtgttcaggg atgaataccg gggtttttaa atacagggaa ggtatatgaa
     7381 aaaggtaaag gttgtttttc tttttatatt gattttttca ggctttcttc tggtcgcatg
     7441 tcaggcaaac tatatccggg atgttcaggg tggaacggtg gcaccatcgt cctcctctga
     7501 actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt
     7561 ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa
     7621 ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac
     7681 caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca accgcgtgtt
     7741 tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag tggcgttttg
     7801 cgtgcttttc cgggttggac tcaagtgaac agttaccgga taaggcgcag cagtcgggct
     7861 gaacgggggg ttcttgctta cagcccagct tggagcgaac gacctacacc gagccgagat
     7921 accagagtgt gagctatgag aaagcgccac acttcccgca agggagaaag gcggaacagg
     7981 tatccgggaa acggcagggt tggaacagga gagcgcaaga gggagcgatt catcggaaac
     8041 ggtgatgatc tttaagtcct gtcgggtttc gccactcctg actgattcat ggttgagcca
     8101 ccggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactcagtc aggagggcgg
     8161 agcctatgga aaaacgccac ctgcgtggcc ttgccgtttt gcctcacatg ttagtcccct
     8221 gcttattcac ggaatctgtg ggtaactttg catgtgtccg cagcgcccgc cgcagtctca
     8281 cggccggagc gtagcgaccg agtgagcgag gaggcgcaat ttccccggca cctacaagac
     8341 ggacaccgga cgcgctgatg tggcatattg tcccctgatt aagtctttat attccctgat
     8401 aataaattac caggataggt gaatcatatc ggtttttcgc tgaacatttt acgttaacct
     8461 atctctgttg cttttttctc cttacggaga aatacaggtc atccccgcga gggcgtggct
     8521 gacctcaggg ggatatcccc tgcttacgct ggctggacgc caccgacgca cggtctctcc
     8581 ccctctttct ggctccgtcc ggctggacgc cggccacgcc agaaattcac ccggcccccg
     8641 tcaggctcac gcctgaccac ccacaaaaat ccacggtgct ggacgcacct ggcgaagcag
     8701 caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg
     8761 ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt
     8821 gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg
     8881 tggtgaaagc tgaacgcaat caccacggac gtgaccgccg ccgccatgcc ttggggcatg
     8941 tccgcggctt gtgcagcggg ggctgcacat cacgccggca aaccatcgtc tgaaggagag
     9001 agaacatgtt taatacggat tcgcttgcct ggaagtggca cattccggag ctggcccggc
     9061 agatttatcc gtgggtcagc tggctgtacc aggagtatta cggtctggcc ggggctttcc
     9121 tagttatcgg cctgtttttc ggtctggctg aactttgggc cggacggatg cgttttgtgg
     9181 ggctgagtca taaggagcga cgggagtatg ccgatcgcta taagcgcgat aataaacggc
     9241 cagcgccatt ttcggtgctg gcctcgctgg attcgcccgg gcggatggtc agggcacgtc
     9301 gacatgtctg gaactacgcg tactggctga ccggcgcggc cggtctggcc cttctggggg
     9361 ggctggttgt cgccgtgttt atcgagagct ggggagaaac cgtcctgcgt ccggacctct
     9421 acgcaaacgc agattattac ctgctgcagc cggctaacct gttcgggatt gcctggtgcg
     9481 cgggcgggct ggctgtcggg attgtgttgg cgcggctgct ggcgttcctg ctgcttgacg
     9541 gtcattttgc ggctgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca
     9601 gcagccagcg gacaggagag atgaccgacg tgcgccacct gcatttcggg gagccggtac
     9661 cggttaatgc gctggccgat ttttcctcgg agcaggcccg gcaggagcag gcggtgtttc
     9721 tgggtaagga tgagcagggc aagccggtgc tggttccgcg tgatacctgg cgcaagacca
     9781 acattcagat cctcggcctg cccgggagcg gcaagagcgt gatggccacc aacgcgctca
     9841 ttcgctgcgt acgcgatttc ggggacgcgg tggtgtactt cgaccccaaa ggggacgcat
     9901 gggcgccgca cgtcttccgg gcgcactgtc cggacttcac cctgctggat ctgcggccgg
     9961 ggaaaccggc gcagctgaat ctgttccgcg atctcgacca gtacgcgctg aaaaacctgc
    10021 tgattgccgg gcttaacctg ggcgagaccg gcgacgtggc ggaccactac c
//
