LOCUS       CP052447                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0098 plasmid pC16KP0098-4,
            complete sequence.
ACCESSION   CP052447
VERSION     CP052447.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609888
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:01:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,695
            CDSs (total)                      :: 5,570
            Genes (coding)                    :: 5,423
            CDSs (with protein)               :: 5,423
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 64 of 147
            Pseudo Genes (incomplete)         :: 78 of 147
            Pseudo Genes (internal stop)      :: 22 of 147
            Pseudo Genes (multiple problems)  :: 16 of 147
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0098"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0098-4"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            299..1984
                     /locus_tag="HJX00_28380"
     CDS             299..1984
                     /locus_tag="HJX00_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJL15814.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            1994..2251
                     /locus_tag="HJX00_28385"
     CDS             1994..2251
                     /locus_tag="HJX00_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJL15815.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            2336..2485
                     /locus_tag="HJX00_28390"
     CDS             2336..2485
                     /locus_tag="HJX00_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJL15816.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3966..5930
                     /locus_tag="HJX00_28395"
     CDS             3966..5930
                     /locus_tag="HJX00_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJL15817.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            5930..6661
                     /locus_tag="HJX00_28400"
     CDS             5930..6661
                     /locus_tag="HJX00_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJL15818.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            6668..7198
                     /locus_tag="HJX00_28405"
     CDS             6668..7198
                     /locus_tag="HJX00_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL15819.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7226..7405)
                     /locus_tag="HJX00_28410"
     CDS             complement(7226..7405)
                     /locus_tag="HJX00_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL15820.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7474..7851
                     /locus_tag="HJX00_28415"
     CDS             7474..7851
                     /locus_tag="HJX00_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJL15821.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(7913..8212)
                     /locus_tag="HJX00_28420"
     CDS             complement(7913..8212)
                     /locus_tag="HJX00_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJL15822.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8202..8465)
                     /locus_tag="HJX00_28425"
     CDS             complement(8202..8465)
                     /locus_tag="HJX00_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJL15823.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(8771..9190)
                     /gene="exc2"
                     /locus_tag="HJX00_28430"
     CDS             complement(8771..9190)
                     /gene="exc2"
                     /locus_tag="HJX00_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJL15824.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     BGC             1..6984
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2589..3167)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5931..6658
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 agaccccccg tttcttttcc tgtcccggag ggggctttct ggtatctcat ataatgattt
       61 cagtaaaaca tactgaggcc ggagaacggc gccgtttgtg ctggtttttg ttgcatctgg
      121 aggggattgc gcatggtgtg agtggctgtt tttttgatca ttttcacaaa aacgcaaatt
      181 tacagacttg acatgaaaaa caaggcagcg taatttttat actgtatata aaaccagtgg
      241 ttatatgtac agtattttat tttagtttat tgttttaaaa gtcaaagagg aaaagtatat
      301 gagtggcgga gacggacgag gtccgggtaa ttcaggtctg ggacacaatg gtggtcaggc
      361 cagtgggaat gtgaacggta catcaggtaa aggtggccct tcatcaggtg gtggtacgga
      421 tccaaacagc gggccgggct ggggtacgac gcatacacct aacggggata ttcataacta
      481 taacccgggg gagtttggta acggcgggag taaacccggt ggtaatggcg gtaacagcgg
      541 caatcatagc gggagctctg gtggcggaca gtcttcggcc accgcgatgg ccttcggtct
      601 gcctgccctg gccactccgg gggctgaagg actggcttta tccgtttccg gcgatgcgtt
      661 gtcggccgct gttgctgatg tgctggctgc cctgaaaggt ccgtttaagt ttggtctgtg
      721 ggggattgcg atttacggag tgctgccttc tgagatagca aaagatgatc cgaaaatgat
      781 gtcaaaaatc gtgacgtcat tgccggcaga cacggtaacg gagacgccgg tcagctccct
      841 gccgctggac caggcgacgg tcagcgtcac taaacgtgtg gcggatattg tgaaggacga
      901 gcggcagcat attgcggttg tcaccggccg gccaatgagt gttcctgtgg tggatgcgaa
      961 accgacaaaa cgtccggggg tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt
     1021 gagcgtacct aaaggtgttc cggccgcgaa agccccgcca aaaggcatta ttgctgaaaa
     1081 aggtgattca cgtccggctg gttttacggc cggtggtaac tcccgtgagg ccgttattcg
     1141 tttcccgaaa gagagcggac agaaaccggt ctatgtgtcg gtgacggatg ttcttactcc
     1201 ggcacaggta aaacagcgtc tggaggaaga aaagcgtcgc cagcaggcat gggacgctgc
     1261 tcacccggaa gaggggctga aaagagagta tgataaagcg aaagctgagc tggatgctga
     1321 agataaaaat attgcgacct taaacagtcg cattgcatcg acagagaagg cgatccccgg
     1381 tgcaagggct gctgttcagg aagccgataa aaaggtaaaa gaggcagagg caaataaaga
     1441 tgattttgtg acttataacc cgcctcatga atatggctcc gggtggcagg atcaggttcg
     1501 ctatcttgat aaggatattc agaatcagaa tgagaaatta aaagctgccc agacatcttt
     1561 aaacgaaatg aatgaatcct tatccaggga taaggctgcg ctttccgggg cgatggagag
     1621 ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat aaattaaatg aggaaaagaa
     1681 aaaacctcgt aagggaacta aagattacgg ccatgattat tttcctgatc ccaagactga
     1741 agatattaag gggttgggag agttgaaaga gggtaaacct aaaaccccta aacaaggtgg
     1801 tggtggtaag cgggcccgat ggtatggtga taaaaagcgt aaaatttatg aatgggattc
     1861 ccagcacggt gagcttgaag ggtaccgcgc cagtgatggc gaacacctcg gggcattcga
     1921 tccaaaaacg ggtaagcagg ttaaagggcc ggatccaaaa cgaaacatta aaaaatatct
     1981 ttaagaggta aatatggggc ttaaattaca tattcattgg tttgataaga aaaccgaaga
     2041 gtttaaaggt ggtgaatact caaaagactt cggtgatgat ggttctgtca ttgaaagtct
     2101 ggggatgcct ttaaaggata atattaataa tggttggttt gatgttgaaa aatcatgggt
     2161 ttcgatatta cagccacact ttaaaaatgt aatcgatatt agtaaatttg attactttgt
     2221 atcctttgtt taccgggatg gtaactggta agcaaaagtt atcaaggatg atttgaaata
     2281 cagacttccg gtgttcaggg atgaataccg gggtttttaa atacagggaa ggtatatgaa
     2341 aaaggtaaag gttgtttttc tttttatatt gattttttca ggctttcttc tggtcgcatg
     2401 tcaggcaaac tatatccggg atgttcaggg tggaacggtg gcaccatcgt cctcctctga
     2461 actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt
     2521 ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa
     2581 ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac
     2641 caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca accgcgtgtt
     2701 tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag tggcgttttg
     2761 cgtgcttttc cgggttggac tcaagtgaac agttaccgga taaggcgcag cagtcgggct
     2821 gaacgggggg ttcttgctta cagcccagct tggagcgaac gacctacacc gagccgagat
     2881 accagagtgt gagctatgag aaagcgccac acttcccgca agggagaaag gcggaacagg
     2941 tatccgggaa acggcagggt tggaacagga gagcgcaaga gggagcgatt catcggaaac
     3001 ggtgatgatc tttaagtcct gtcgggtttc gccactcctg actgattcat ggttgagcca
     3061 ccggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactcagtc aggagggcgg
     3121 agcctatgga aaaacgccac ctgcgtggcc ttgccgtttt gcctcacatg ttagtcccct
     3181 gcttattcac ggaatctgtg ggtaactttg catgtgtccg cagcgcccgc cgcagtctca
     3241 cggccggagc gtagcgaccg agtgagcgag gaggcgcaat ttccccggca cctacaagac
     3301 ggacaccgga cgcgctgatg tggcatattg tcccctgatt aagtctttat attccctgat
     3361 aataaattac caggataggt gaatcatatc ggtttttcgc tgaacatttt acgttaacct
     3421 atctctgttg cttttttctc cttacggaga aatacaggtc atccccgcga gggcgtggct
     3481 gacctcaggg ggatatcccc tgcttacgct ggctggacgc caccgacgca cggtctctcc
     3541 ccctctttct ggctccgtcc ggctggacgc cggccacgcc agaaattcac ccggcccccg
     3601 tcaggctcac gcctgaccac ccacaaaaat ccacggtgct ggacgcacct ggcgaagcag
     3661 caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg
     3721 ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt
     3781 gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg
     3841 tggtgaaagc tgaacgcaat caccacggac gtgaccgccg ccgccatgcc ttggggcatg
     3901 tccgcggctt gtgcagcggg ggctgcacat cacgccggca aaccatcgtc tgaaggagag
     3961 agaacatgtt taatacggat tcgcttgcct ggaagtggca cattccggag ctggcccggc
     4021 agatttatcc gtgggtcagc tggctgtacc aggagtatta cggtctggcc ggggctttcc
     4081 tagttatcgg cctgtttttc ggtctggctg aactttgggc cggacggatg cgttttgtgg
     4141 ggctgagtca taaggagcga cgggagtatg ccgatcgcta taagcgcgat aataaacggc
     4201 cagcgccatt ttcggtgctg gcctcgctgg attcgcccgg gcggatggtc agggcacgtc
     4261 gacatgtctg gaactacgcg tactggctga ccggcgcggc cggtctggcc cttctggggg
     4321 ggctggttgt cgccgtgttt atcgagagct ggggagaaac cgtcctgcgt ccggacctct
     4381 acgcaaacgc agattattac ctgctgcagc cggctaacct gttcgggatt gcctggtgcg
     4441 cgggcgggct ggctgtcggg attgtgttgg cgcggctgct ggcgttcctg ctgcttgacg
     4501 gtcattttgc ggctgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca
     4561 gcagccagcg gacaggagag atgaccgacg tgcgccacct gcatttcggg gagccggtac
     4621 cggttaatgc gctggccgat ttttcctcgg agcaggcccg gcaggagcag gcggtgtttc
     4681 tgggtaagga tgagcagggc aagccggtgc tggttccgcg tgatacctgg cgcaagacca
     4741 acattcagat cctcggcctg cccgggagcg gcaagagcgt gatggccacc aacgcgctca
     4801 ttcgctgcgt acgcgatttc ggggacgcgg tggtgtactt cgaccccaaa ggggacgcat
     4861 gggcgccgca cgtcttccgg gcgcactgtc cggacttcac cctgctggat ctgcggccgg
     4921 ggaaaccggc gcagctgaat ctgttccgcg atctcgacca gtacgcgctg aaaaacctgc
     4981 tgattgccgg gcttaacctg ggcgagaccg gcgacgtggc ggaccactac cgcatcagcg
     5041 agcagaaagc ggcgaagctg attgcggagc agtttccgca gggggcaaac attcagcagg
     5101 tgctggcggc ggcgtacgcg ctgccggagg ccctgaaaaa ggacgtgaag gggttgatca
     5161 ctaggctgga aaacgtcgct gacctgagcg tcctgcagac ggacagtggc atcgatgtgg
     5221 cggggattat caacggcggc ggctgcctgt acgttatcgg ctcgatggac gacgaggcgg
     5281 tgatccgtgt gcaaaaaatg ctgtttgccc gctgcgcgca aatcatcatt gcgcgggacg
     5341 agttccggga gtggccacac gtcagcgtga tgctcgatga aattaaatat ctgctgtcga
     5401 agtatgtact gaacgcactg gggacgctgc gcagccggga ctgcaatctg ttgctcgcgc
     5461 accagtcgct gggcgatttc gggcagtgcg ggcaggatct gcccgctgaa tttgtgaaaa
     5521 ccacggtact ggataacacg ccgatccgct ggttttaccg ggcggccagc ctggagtccg
     5581 cgcagtgggc ggcggggcag accggggaaa tcagggtgga cgtggagcgc cgccgtgcca
     5641 gccgggaggc ggggaacgtg gagcatatca gcggggacag cttcatccag aaggaggcgc
     5701 ggccgctgtt tgacgtgaac accctgcaac acctgccgga cgggttcgcg gtcatgaccg
     5761 ggctgggtgt ggcccggatg gcgttcagca gtccgctgcg ggttgagcgc cgggaaatcc
     5821 cgttgcagcg tttcccggtg ctggcgaaaa ccgatcccct ggcggagttc cggcccccgg
     5881 ccgacggcgg cgggccgaaa gcggatgatg attttgcggg gatttactga tggcgctgga
     5941 gctgtacaac gtcagccacg cgaaacgaca ggcgcggaac gcggagaaaa cccggctgac
     6001 cctgcgctgg ttgcgggaag agctgtgttc cacggcggag ctggttgcgc ggcggctggg
     6061 gattgcggcg gtgcagccgg tctaccgctt cctcgacagc ctggtggcga aagggctgct
     6121 ggtccggacc aaatatccgg tggacggccg tcaggtcagc gtgtgggggc tgacgccgca
     6181 tggcgtggcg ttctcgttcg acgaggacga accgctggcg gatgttattc cgtttcagcc
     6241 gtcaagggtg tcggcagcac atctgccgca ccggctggcc gtgcaatccc tgcgccttga
     6301 tatggaagcc agaggggcca ccggctggcg cattctgcac cgtctgagtc ttaaaggcat
     6361 gaaagtgccg gacgccctgg cggagctgga cggtaaaacg gtggcgtttg aggtggagcg
     6421 caccgtcaag agccgtcgcc ggtatcagga agtggtgtcc gggtatctgt tcaaccgcaa
     6481 agctaacggc atcgatgaaa tctggtacat ctgcccggac cgggcgacgc aggtcagggt
     6541 gcagcgggca atcctgtcgg tggatgaaat cgtgaaccca cagaccggcg aggcccgcaa
     6601 aacggcagag ctggatcgtg aacggttgtt tgcgtgcttt aaatttatga caacggagtg
     6661 agggattatg gcgctgtaca tggtatttat tgaagacgcg gaggaccgga acggcgagcg
     6721 ctgcatcatg ccgaccagcg agcatctgga agatatggtg gtgctcgcct ggcagaccgg
     6781 ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg gcgatgatga aatccatcgg
     6841 gatgtatgac gagcggctca acagcgacga aaaagccacg tactttttca ctggtaaaaa
     6901 tccgcaatgg gaacggaaca aacccacagg aatgaagctg tgtgattacc tctatcgcaa
     6961 ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt gatgccggga agtgctcacc
     7021 ggagcagatt aacgcgatga ttgacgtggt gagtgagcgg cacggggaaa gcgcggtgaa
     7081 cgagaagctg aaagcggagc tgatgcggga atataacagc cgctatttta acgtgccggt
     7141 acctgaactg ccggaggaca aacctgcccg ccggtggtgg ccgttcggca aaaaataaat
     7201 gccagcaccg gccatgccgg tgctgttatt cctccccgag cttatcccgc atcacctgcc
     7261 agagtgtttc cgcatcctcg tgtagccgct cgcacatgtc ggccggggta tccatatcca
     7321 gctcattcag tttatcgagc aatagcagcg actgctcctt aataaattta gccatattga
     7381 gggctgtttt ttcctgtttt gtcatgattc tttccttccg ggcatggtgt gcgcctgtca
     7441 ggtatactgt ctcaaaaata gcggggtgag acaatggcgt gggtgacggt gagacaggcg
     7501 acggaactga cgggtaaagc ccgaagttcc ctgtatcggg atatggcgaa aggccgcgta
     7561 tcataccgta cggaagctga tggcggccgg gtggtggata ccagcgagct tatccgggtg
     7621 tacggtgagt taagacagga tgagactcac gagcgggacg agttgagact gtcgggtgag
     7681 actgaaaaaa tggcctccga cgcgttgatc gcggagataa aagcactcag ggaggaggtg
     7741 gccgggctac ggcaggaaat gcagtccatg agactgctgg aacacaaacc ggagacagca
     7801 gagaccccgg ccggaacacc gcgccggtgg tggccgtggg gaaaacgctg acgcgctgtg
     7861 cttgtccaac cggcgacggc cacgcaatga attgcgtccc cgtcaccggt tattagagaa
     7921 caatatgggg attctggcgc tggccgacca cggccagaac ccggactttt ctggtctggt
     7981 catcaacatc atagaggaca tgctgtccca tcagactgat ccggcgaacg tcctcgccat
     8041 agcgtgagtc cacttcatag agtcgcggga agtcggccag tttgtccgcc gcagcgagaa
     8101 cccggtcggt aacgttctgc gcatcccaca gggatgaatt ttccgcgata tagtcaaaca
     8161 gcgtcgttac atcctcgatg gctccctgct tccattccac gtcatacggc atcgcgcagt
     8221 tcctgataac ggccatgcag gcgctggcgg agttgttccg tggtcatacc cggacgcgtg
     8281 tcagcacggg cggcggtcac tttggcgcga acctgatctt catactctgc ctgggccagt
     8341 tcttcgttag ccagtcgctg caacagcgca gctttgtccg ggccaccttc cggcagtatg
     8401 cggggcaggc tttcagccag ttcccgaaga aagttggcgt tttcgtagtg tgtagtgttg
     8461 ctcattgcgg agcctcccgg gttaatgcgg caatatgaag gaagtttagc agggggcggt
     8521 ctgaaagaat atggtcgtgt cagtcaggct gatgaagcag aaagtcggta aaaaagggct
     8581 tacggcatcc attttacgtc aaaacatatg ccatgcagtt gcgtgcatag gcatgcatta
     8641 ggataaaatt tatcgggcgc gtttccggca gttttccggg ggggttgttg cctgttttat
     8701 cccgtagccg ccggaaacgc cctgagcccg tctgagcggt gcgcgtaatg acgcgttatg
     8761 gtaaatccac ttactgtctg ccttcatagc catcgagata aacagctgaa atggtgtcag
     8821 ccagtagcct tgattttttc ggtgtgaggt ctttgctgcg gtaatcggta cccgcaaatt
     8881 gtgttgtgcc ttggagtgag tttaaaaatt cctgactgtg aaactgctga atgcgttgct
     8941 gcacatcact ccgtgatttt ccggtcgctt tatcctttgc gcccatatcc cagaactgcc
     9001 ggaaaaaagg caccatcatt cgtgttgtgt cagctttctg ggtggaaaaa ttgggatcaa
     9061 aaccgtcatc tgaagcatat acgtaatgcc gggtatgctg ttccacgctg ccctttggag
     9121 cacaggcagt gagagttgct gtcgctgtta tcatggcgat aagacacagt ttgctgccgg
     9181 aacgtctcat gagcgtctcc ttacctttaa ttcttttttc tcattgagtt atatatccgc
     9241 aggctatctt atcatcagtt tctgaccgtg ttcagtgacc gaacaacccg aacc
//
