LOCUS       CP052428                9295 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0129 plasmid pC16KP0129-1,
            complete sequence.
ACCESSION   CP052428
VERSION     CP052428.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609892
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:38:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,290
            CDSs (total)                      :: 5,168
            Genes (coding)                    :: 5,049
            CDSs (with protein)               :: 5,049
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 86 of 119
            Pseudo Genes (incomplete)         :: 23 of 119
            Pseudo Genes (internal stop)      :: 17 of 119
            Pseudo Genes (multiple problems)  :: 6 of 119
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0129"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0129-1"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(9059..9295,1..495)
                     /locus_tag="HJX04_26360"
     CDS             join(9059..9295,1..495)
                     /locus_tag="HJX04_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJK99740.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            502..1032
                     /locus_tag="HJX04_26365"
     CDS             502..1032
                     /locus_tag="HJX04_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK99741.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1060..1239)
                     /locus_tag="HJX04_26370"
     CDS             complement(1060..1239)
                     /locus_tag="HJX04_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK99742.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1308..1685
                     /locus_tag="HJX04_26375"
     CDS             1308..1685
                     /locus_tag="HJX04_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJK99743.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(1747..2046)
                     /locus_tag="HJX04_26380"
     CDS             complement(1747..2046)
                     /locus_tag="HJX04_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJK99744.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2036..2299)
                     /locus_tag="HJX04_26385"
     CDS             complement(2036..2299)
                     /locus_tag="HJX04_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJK99745.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(2605..3024)
                     /gene="exc2"
                     /locus_tag="HJX04_26390"
     CDS             complement(2605..3024)
                     /gene="exc2"
                     /locus_tag="HJX04_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJK99746.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            3427..5112
                     /locus_tag="HJX04_26395"
     CDS             3427..5112
                     /locus_tag="HJX04_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK99747.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            5122..5379
                     /locus_tag="HJX04_26400"
     CDS             5122..5379
                     /locus_tag="HJX04_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK99748.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            5464..5613
                     /locus_tag="HJX04_26405"
     CDS             5464..5613
                     /locus_tag="HJX04_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJK99749.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7094..9059
                     /locus_tag="HJX04_26410"
                     /pseudo=""
     CDS             7094..9059
                     /locus_tag="HJX04_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /name="TraM recognition domain-containing protein"
     BGC             1..9295
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5717..6295)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9060..9295
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.82e-33"
ORIGIN
        1 gggctgacgc cgcatggcgt ggcgttctcg ttcgacgagg acgaaccgct ggcggatgtt
       61 attccgtttc agccgtcaag ggtgtcggca gcacatctgc cgcaccggct ggccgtgcaa
      121 tccctgcgcc ttgatatgga agccagaggg gccaccggct ggcgcattct gcaccgtctg
      181 agtcttaaag gcatgaaagt gccggacgcc ctggcggagc tggacggtaa aacggtggcg
      241 tttgaggtgg agcgcaccgt caagagccgt cgccggtatc aggaagtggt gtccgggtat
      301 ctgttcaacc gcaaagctaa cggcatcgat gaaatctggt acatctgccc ggaccgggcg
      361 acgcaggtca gggtgcagcg ggcaatcctg tcggtggatg aaatcgtgaa cccacagacc
      421 ggcgaggccc gcaaaacggc agagctggat cgtgaacggt tgtttgcgtg ctttaaattt
      481 atgacaacgg agtgagggat tatggcgctg tacatggtat ttattgaaga cgcggaggac
      541 cggaacggcg agcgctgcat catgccgacc agcgagcatc tggaagatat ggtggtgctc
      601 gcctggcaga ccggggcgta tgttttctgt tttgtcgacc gggcccagca tctggcgatg
      661 atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt
      721 ttcactggta aaaatccgca atgggaacgg aacaaaccca caggaatgaa gctgtgtgat
      781 tacctctatc gcaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt ggttgatgcc
      841 gggaagtgct caccggagca gattaacgcg atgattgacg tggtgagtga gcggcacggg
      901 gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa cagccgctat
      961 tttaacgtgc cggtacctga actgccggag gacaaacctg cccgccggtg gtggccgttc
     1021 ggcaaaaaat aaatgccagc accggccatg ccggtgctgt tattcctccc cgagcttatc
     1081 ccgcatcacc tgccagagtg tttccgcatc ctcgtgtagc cgctcgcaca tgtcggccgg
     1141 ggtatccata tccagctcat tcagtttatc gagcaatagc agcgactgct ccttaataaa
     1201 tttagccata ttgagggctg ttttttcctg ttttgtcatg attctttcct tccgggcatg
     1261 gtgtgcgcct gtcaggtata ctgtctcaaa aatagcgggg tgagacaatg gcgtgggtga
     1321 cggtgagaca ggcgacggaa ctgacgggta aagcccgaag ttccctgtat cgggatatgg
     1381 cgaaaggccg cgtatcatac cgtacggaag ctgatggcgg ccgggtggtg gataccagcg
     1441 agcttatccg ggtgtacggt gagttaagac aggatgagac tcacgagcgg gacgagttga
     1501 gactgtcggg tgagactgaa aaaatggcct ccgacgcgtt gatcgcggag ataaaagcac
     1561 tcagggagga ggtggccggg ctacggcagg aaatgcagtc catgagactg ctggaacaca
     1621 aaccggagac agcagagacc ccggccggaa caccgcgccg gtggtggccg tggggaaaac
     1681 gctgacgcgc tgtgcttgtc caaccggcga cggccacgca atgaattgcg tccccgtcac
     1741 cggttattag agaacaatat ggggattctg gcgctggccg accacggcca gaacccggac
     1801 ttttctggtc tggtcatcaa catcatagag gacatgctgt cccatcagac tgatccggcg
     1861 aacgtcctcg ccatagcgtg agtccacttc atagagtcgc gggaagtcgg ccagtttgtc
     1921 cgccgcagcg agaacccggt cggtaacgtt ctgcgcatcc cacagggatg aattttccgc
     1981 gatatagtca aacagcgtcg ttacatcctc gatggctccc tgcttccatt ccacgtcata
     2041 cggcatcgcg cagttcctga taacggccat gcaggcgctg gcggagttgt tccgtggtca
     2101 tacccggacg cgtgtcagca cgggcggcgg tcactttggc gcgaacctga tcttcatact
     2161 ctgcctgggc cagttcttcg ttagccagtc gctgcaacag cgcagctttg tccgggccac
     2221 cttccggcag tatgcggggc aggctttcag ccagttcccg aagaaagttg gcgttttcgt
     2281 agtgtgtagt gttgctcatt gcggagcctc ccgggttaat gcggcaatat gaaggaagtt
     2341 tagcaggggg cggtctgaaa gaatatggtc gtgtcagtca ggctgatgaa gcagaaagtc
     2401 ggtaaaaaag ggcttacggc atccatttta cgtcaaaaca tatgccatgc agttgcgtgc
     2461 ataggcatgc attaggataa aatttatcgg gcgcgtttcc ggcagttttc cgggggggtt
     2521 gttgcctgtt ttatcccgta gccgccggaa acgccctgag cccgtctgag cggtgcgcgt
     2581 aatgacgcgt tatggtaaat ccacttactg tctgccttca tagccatcga gataaacagc
     2641 tgaaatggtg tcagccagta gccttgattt tttcggtgtg aggtctttgc tgcggtaatc
     2701 ggtacccgca aattgtgttg tgccttggag tgagtttaaa aattcctgac tgtgaaactg
     2761 ctgaatgcgt tgctgcacat cactccgtga ttttccggtc gctttatcct ttgcgcccat
     2821 atcccagaac tgccggaaaa aaggcaccat cattcgtgtt gtgtcagctt tctgggtgga
     2881 aaaattggga tcaaaaccgt catctgaagc atatacgtaa tgccgggtat gctgttccac
     2941 gctgcccttt ggagcacagg cagtgagagt tgctgtcgct gttatcatgg cgataagaca
     3001 cagtttgctg ccggaacgtc tcatgagcgt ctccttacct ttaattcttt tttctcattg
     3061 agttatatat ccgcaggcta tcttatcatc agtttctgac cgtgttcagt gaccgaacaa
     3121 cccgaaccag accccccgtt tcttttcctg tcccggaggg ggctttctgg tatctcatat
     3181 aatgatttca gtaaaacata ctgaggccgg agaacggcgc cgtttgtgct ggtttttgtt
     3241 gcatctggag gggattgcgc atggtgtgag tggctgtttt tttgatcatt ttcacaaaaa
     3301 cgcaaattta cagacttgac atgaaaaaca aggcagcgta atttttatac tgtatataaa
     3361 accagtggtt atatgtacag tattttattt tagtttattg ttttaaaagt caaagaggaa
     3421 aagtatatga gtggcggaga cggacgaggt ccgggtaatt caggtctggg acacaatggt
     3481 ggtcaggcca gtgggaatgt gaacggtaca tcaggtaaag gtggcccttc atcaggtggt
     3541 ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacacctaa cggggatatt
     3601 cataactata acccggggga gtttggtaac ggcgggagta aacccggtgg taatggcggt
     3661 aacagcggca atcatagcgg gagctctggt ggcggacagt cttcggccac cgcgatggcc
     3721 ttcggtctgc ctgccttggc cactccgggg gctgaaggac tggctttatc cgtttccggc
     3781 gatgcgttgt cggccgctgt tgctgatgtg ctggctgccc tgaaaggtcc gtttaagttt
     3841 ggtctgtggg ggattgcgat ttacggagtg ctgccttctg agatagcaaa agatgatccg
     3901 aaaatgatgt caaaaatcgt gacgtcattg ccggcagaca cggtaacgga gacgccggtc
     3961 agctccctgc cgctggacca ggcgacggtc agcgtcacta aacgtgtggc ggatattgtg
     4021 aaggacgagc ggcagcatat tgcggttgtc accggccggc caatgagtgt tcctgtggtg
     4081 gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct
     4141 ctgcaggtga gcgtacctaa aggtgttccg gccgcgaaag ccccgccaaa aggcattatt
     4201 gctgaaaaag gtgattcacg tccggctggt tttacggccg gtggtaactc ccgtgaagcc
     4261 gttattcgtt tcccgaaaga gagcggacag aaaccggtct atgtgtcggt gacggatgtt
     4321 cttactccgg cacaggtaaa acagcgtctg gaggaagaaa agcgtcgcca gcaggcatgg
     4381 gacgctgctc acccggaaga ggggctgaaa agagagtatg ataaagcgaa agctgagctg
     4441 gatgctgaag ataaaaatat tgcgacctta aacagtcgca ttgcatcgac agagaaggcg
     4501 atccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca
     4561 aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat
     4621 caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa agctgcccag
     4681 acatctttaa acgaaatgaa tgaatcctta tccagggata aggctgcgct ttccggggcg
     4741 atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa attaaatgag
     4801 gaaaagaaaa aacctcgtaa gggaactaaa gattacggcc atgattattt tcctgatccc
     4861 aagactgaag atattaaggg gttgggagag ttgaaagagg gtaaacctaa aacccctaaa
     4921 caaggtggtg gtggtaagcg ggcccgatgg tatggtgata aaaagcgtaa aatttatgaa
     4981 tgggattccc agcacggtga gcttgaaggg taccgcgcca gtgatggcga acacctcggg
     5041 gcattcgatc caaaaacggg taagcaggtt aaagggccgg atccaaaacg aaacattaaa
     5101 aaatatcttt aagaggtaaa tatggggctt aaattacata ttcattggtt tgataagaaa
     5161 accgaagagt ttaaaggtgg tgaatactca aaagacttcg gtgatgatgg ttctgtcatt
     5221 gaaagtctgg ggatgccttt aaaggataat attaataatg gttggtttga tgttgaaaaa
     5281 tcatgggttt cgatattaca gccacacttt aaaaatgtaa tcgatattag taaatttgat
     5341 tactttgtat cctttgttta ccgggatggt aactggtaag caaaagttat caaggatgat
     5401 ttgaaataca gacttccggt gttcagggat gaataccggg gtttttaaat acagggaagg
     5461 tatatgaaaa aggtaaaggt tgtttttctt tttatattga ttttttcagg ctttcttctg
     5521 gtcgcatgtc aggcaaacta tatccgggat gttcagggtg gaacggtggc accatcgtcc
     5581 tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc
     5641 ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt
     5701 tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc
     5761 acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac
     5821 cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg
     5881 gcgttttgcg tgcttttccg ggttggactc aagtgaacag ttaccggata aggcgcagca
     5941 gtcgggctga acggggggtt cttgcttaca gcccagcttg gagcgaacga cctacaccga
     6001 gccgagatac cagagtgtga gctatgagaa agcgccacac ttcccgcaag ggagaaaggc
     6061 ggaacaggta tccgggaaac ggcagggttg gaacaggaga gcgcaagagg gagcgattca
     6121 tcggaaacgg tgatgatctt taagtcctgt cgggtttcgc cactcctgac tgattcatgg
     6181 ttgagccacc ggctcccaca gatgcaccga aaaagcgtct gtttatgtga actcagtcag
     6241 gagggcggag cctatggaaa aacgccacct gcgtggcctt gccgttttgc ctcacatgtt
     6301 agtcccctgc ttattcacgg aatctgtggg taactttgca tgtgtccgca gcgcccgccg
     6361 cagtctcacg gccggagcgt agcgaccgag tgagcgagga ggcgcaattt ccccggcacc
     6421 tacaagacgg acaccggacg cgctgatgtg gcatattgtc ccctgattaa gtctttatat
     6481 tccctgataa taaattacca ggataggtga atcatatcgg tttttcgctg aacattttac
     6541 gttaacctat ctctgttgct tttttctcct tacggagaaa tacaggtcat ccccgcgagg
     6601 gcgtggctga cctcaggggg atatcccctg cttacgctgg ctggacgcca ccgacgcacg
     6661 gtctctcccc ctctttctgg ctccgtccgg ctggacgccg gccacgccag aaattcaccc
     6721 ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg acgcacctgg
     6781 cgaagcagca aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct
     6841 gacgggggcc ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc
     6901 tggggcgtgc tcgttgttcc tccggggctg cgccttttcc gcatggggcg gaaagaggct
     6961 ggcctccgtg gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc gccatgcctt
     7021 ggggcatgtc cgcggcttgt gcagcggggg ctgcacatca cgccggcaaa ccatcgtctg
     7081 aaggagagag aacatgttta atacggattc gcttgcctgg aagtggcaca ttccggagct
     7141 ggcccggcag atttatccgt gggtcagctg gctgtaccag gagtattacg gtctggccgg
     7201 ggctttccta gttatcggcc tgtttttcgg tctggctgaa ctttgggccg gacggatgcg
     7261 ttttgtgggg ctgagtcata aggagcgacg ggagtatgcc gatcgctata agcgcgataa
     7321 taaacggcca gcgccatttt cggtgctggc ctcgctggat tcgcccgggc ggatggtcag
     7381 ggcacgtcga catgtctgga actacgcgta ctggctgacc ggcgcggccg gtctggccct
     7441 tctggggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc gtcctgcgtc
     7501 cggacctcta cgcaaacgca gattattacc tgctgcagcc ggctaacctg ttcgggattg
     7561 cctggtgcgc gggcgggctg gctgtcggga ttgtgttggc gcggctgctg gcgttcctgc
     7621 tgcttgacgg tcattttgcg gctgctgacg aggcggtgaa cgcgaaactg aatcaggaga
     7681 gccggcgcag cagccagcgg acaggagaga tgaccgacgt gcgccacctg catttcgggg
     7741 agccggtacc ggttaatgcg ctggccgatt tttcctcgga gcaggcccgg caggagcagg
     7801 cggtgtttct gggtaaggat gagcagggca agccggtgct ggttccgcgt gatacctggc
     7861 gcaagaccaa cattcagatc ctcggcctgc ccgggagcgg caagagcgtg atggccacca
     7921 acgcgctcat tcgctgcgta cgcgatttcg gggacgcggt ggtgtacttc gaccccaaag
     7981 gggacgcatg ggcgccgcac gtcttccggg cgcactgtcc ggacttcacc ctgctggatc
     8041 tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag tacgcgctga
     8101 aaaacctgct gattgccggg cttaacctgg gcgagaccgg cgacgtggcg gaccactacc
     8161 gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gtttccgcag ggggcaaaca
     8221 ttcagcaggt gctggcggcg gcgtacgcgc tgccggaggc cctgaaaaag gacgtgaagg
     8281 ggttgatcac taggctggaa aacgtcgctg acctgagcgt cctgcagacg gacagtggca
     8341 tcgatgtggc ggggattatc aacggcggcg gctgcctgta cgttatcggc tcgatggacg
     8401 acgaggcggt gatccgtgtg caaaaaatgc tgtttgcccg ctgcgcgcaa atcatcattg
     8461 cgcgggacga gttccgggag tggccacacg tcagcgtgat gctcgatgaa attaaatatc
     8521 tgctgtcgaa gtatgtactg aacgcactgg ggacgctgcg cagccgggac tgcaatctgt
     8581 tgctcgcgca ccagtcgctg ggcgatttcg ggcagtgcgg gcaggatctg cccgctgaat
     8641 ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttttaccgg gcggccagcc
     8701 tggagtccgc gcagtgggcg gcggggcaga ccggggaaat cagggtggac gtggagcgcc
     8761 gccgtgccag ccgggaggcg gggaacgtgg agcatatcag cggggacagc ttcatccaga
     8821 aggaggtgcg gccgctgttt gacgtgaaca ccctgcaaca cctgccggac gggttcgcgg
     8881 tcatgaccgg gctgggtgtg gcccggatgg cgttcagcag tccgctgcgg gttgagcgcc
     8941 gggaaatccc gttgcagcgt ttcccggtgc tggcgaaaac cgatcccctg gcggagttcc
     9001 ggcccccggc cgacggcggc gggccgaaag cggatgatga ttttgcgggg atttactgat
     9061 ggcgctggag ctgtacaacg tcagccacgc gaaacgacag gcgcggaacg cggagaaaac
     9121 ccggctgacc ctgcgctggt tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg
     9181 gcggctgggg attgcggcgg tgcagccggt ctaccgcttc ctcgacagcc tggtggcgaa
     9241 agggctgctg gtccggacca aatatccggt ggacggccgt caggtcagcg tgtgg
//
