LOCUS       CP052425                2789 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0164 plasmid pC16KP0164-3,
            complete sequence.
ACCESSION   CP052425
VERSION     CP052425.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609894
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:56:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,331
            Genes (coding)                    :: 5,192
            CDSs (with protein)               :: 5,192
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 73 of 139
            Pseudo Genes (incomplete)         :: 63 of 139
            Pseudo Genes (internal stop)      :: 22 of 139
            Pseudo Genes (multiple problems)  :: 18 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0164"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0164-3"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(638..1039)
                     /locus_tag="HJX06_27240"
     CDS             complement(638..1039)
                     /locus_tag="HJX06_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJK84067.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1039..1230)
                     /locus_tag="HJX06_27245"
     CDS             complement(1039..1230)
                     /locus_tag="HJX06_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK84068.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFCTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1187..1610
                     /locus_tag="HJX06_27250"
                     /pseudo=""
     CDS             1187..1610
                     /locus_tag="HJX06_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            1635..1766
                     /locus_tag="HJX06_27255"
     CDS             1635..1766
                     /locus_tag="HJX06_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJK84069.1"
                     /translation="MHEPPVQSDYHALFGNYRLESNPKRHDKSPLAVAIGRGCTRDY"
                     /name="replication initiation protein"
     gene            complement(2057..2476)
                     /locus_tag="HJX06_27260"
     CDS             complement(2057..2476)
                     /locus_tag="HJX06_27260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK84070.1"
                     /translation="MKLSLFFISVITFLLAISPIAYSDNTIIPDRVYDPYRNSAVTPDG
                     TVYDRLTGGVLKASEHPQPNFGFPVQSSSDSCDTTYVKARTEDGRLLVTSDGVTLHVVD
                     NIVDVAHWQPNDELRVCGDRVINIDDNTSSSFTYN"
                     /name="hypothetical protein"
     gene            complement(2473..2670)
                     /locus_tag="HJX06_27265"
     CDS             complement(2473..2670)
                     /locus_tag="HJX06_27265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK84071.1"
                     /translation="MERVLGIYVVGIIVSAGFLFIQEVRNGRRRSYTEEGASMLVGIIC
                     AFLWPLVLIAYFFDKKNGKL"
                     /name="hypothetical protein"
     Typing          1454..1963
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.038"
                     /coverage="99.0"
                     /evalue="1.71e-152"
ORIGIN
        1 cagaaacgcg caggctctgt ttttaaggca tgcatgagta tgcacgcaag tgcatagcgc
       61 gaaaattcac gccattcagc cagagtaagt cctgtttccg gcgttctttc catccgaaaa
      121 cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa
      181 ccggttttca gcacgttcag gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa
      241 gtcgccggac gactcaccac acgattcacc ggcgattcga atcgtgaatt gaatcaagag
      301 tcaccatgag caaaaaacac ccaaagtgat gaattgaatc aagagtcgat tcatcataac
      361 aatatgataa taaagcgtga ttcacatatg aatcgtgggc aattcgtcag cgattcgcca
      421 tagagtcgtc cacgacttgg atctgcactt tgtaacacat caccgcacat ttatttttca
      481 ttaacccatt gttgtaaata aggaatctgc aaaattttaa ttttgcgtgg ggtgtgggta
      541 tttttagtgg tgaggagcgt tcagagatct gacgattttt gggaaggttc aggaactccc
      601 tgggatggcc ggagaggaaa actgaagcat gacggggtca ggattttttc cagaatttcc
      661 accatggcgc cggggattca gttaattttg cctggtgatt ttcaagaagg agcatggcct
      721 gtttcaggct gtcaatgtgc gaatccttgg acaaaagtaa ggtgttcagg tgttcaatct
      781 cctgtttcag atgacgaatc acctgttcac tctggtcagc agaggctgta tcacgctgtt
      841 cagagacctg tccagaatgg tgttcaccct tcagttgttc aaaggggcca aagacacgta
      901 aaagctctga agtatctacc atttttgcat tacgttcacc gctacaggtg gacaggtcac
      961 ctgctttaat taacctgtgc agtgttgttc tactcttacc cgtcagtcgg gcagcctcac
     1021 taatagacac tctggccatc agttaccatc ctgtaatgca tccagtctcg cggtcagtgt
     1081 acagaaaaga tgctccgcat catcgtggag tttctcacaa aggtcggcct cgacatcgag
     1141 atcaagctcg ttaagcttct ccagcaggag cagagactga ttctggatga atttagccat
     1201 gttcagcgct gttttctgtt gtttcgtcat aaagggcctc agagcgtcag tgctcaatgt
     1261 cagacagatc tcttttccgc ttcctcgctc actcagtcgc tacgctcctg ccgttcggct
     1321 gcggcgagcg gtcgcaacgc gcacataacg acatagagca gacagctcgg agagggagaa
     1381 ggcagcacag cggccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     1441 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1501 tgatggctcc ctcgtgcgct ctcctgttcc tgccattcgg tttaccggtg tcattccgct
     1561 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     1621 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctaccacgct cttttcggta
     1681 actatcgtct tgagtccaac ccgaaaaggc acgacaaatc gccattggcg gtagccattg
     1741 gtagagggtg tacaagagat tactgatgca aagttcttga agttagagcc agactgcggc
     1801 taaactgaaa ggacgaattt ggtggcagcg gtcttgtaca ccctgttacc acggttcaga
     1861 agttccccaa cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttca
     1921 gagcaagaga ttacgacgct cctgaaagga tctcaagaag atccttttga actttggttc
     1981 atttcttcgg ctatcccccc atacatgctc gtcaatcttt ctatcatcac catagatatt
     2041 ctttgaataa aagaggttaa ttgtatgtga acgaggagga agtattgtca tcaatattga
     2101 ttactctatc gccacagact ctaagctcat catttggttg ccagtgagca acatcaacaa
     2161 tattgtcaac tacatgcaga gttactccgt cgctcgtgac taaaagccta ccgtcttctg
     2221 tcctagcttt aacatacgtt gtatcacaac tatccgatga ggattgaaca ggaaacccaa
     2281 aattaggttg agggtgctct gatgccttca aaactcctcc tgtcagacgg tcataaacgg
     2341 tgccatcagg agttacagcc gaatttctat aaggatcata tactctgtca ggaataatcg
     2401 tgttatctga gtatgctatc ggtgatatag ccaataaaaa agtaatgacc gagatgaaaa
     2461 acagacttaa cttcataatt tcccattttt tttatcaaaa aaatatgcaa ttaaaactaa
     2521 aggccacaaa aacgcacaaa ttatgccgac aagcattgac gcaccttctt cggtgtaact
     2581 tctgcgcctc ccgttcctaa cttcttggat aaacaggaaa ccggcactca caataatacc
     2641 gacaacataa attcctaata ccctttccat aaaacattcc gttcttatca gattaaacat
     2701 aacatccctt gtacgcaccg ctaaaacggt ctcagggcgt ttctgagggg aaaccggtaa
     2761 aacaggcaac aaaccacccg aaaacccgc
//
