LOCUS       CP052420                2927 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0189 plasmid pC16KP0189-6,
            complete sequence.
ACCESSION   CP052420
VERSION     CP052420.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609895
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:08:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,713
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 70 of 131
            Pseudo Genes (incomplete)         :: 63 of 131
            Pseudo Genes (internal stop)      :: 18 of 131
            Pseudo Genes (multiple problems)  :: 19 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0189"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0189-6"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(171..605)
                     /locus_tag="HJX07_28535"
     CDS             complement(171..605)
                     /locus_tag="HJX07_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="QJL05207.1"
                     /translation="MNLAMFSAVFCCFVIKGLRASVLSVKPVSFPLPRSLSRYAPVVRL
                     RRAAGACIEKLEKTERSKEEKKERPRRCRFSIGSAPLTSIAETDAQISGGETRQDYKDT
                     RRFPLAAPSCALLFLPFGLPVSLRCYGRVYLIPRLTLSSG"
                     /name="plasmid replication initiation protein"
     gene            562..753
                     /locus_tag="HJX07_28540"
     CDS             562..753
                     /locus_tag="HJX07_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL05208.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            753..1175
                     /locus_tag="HJX07_28545"
     CDS             753..1175
                     /locus_tag="HJX07_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL05209.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1521..1763)
                     /locus_tag="HJX07_28550"
     CDS             complement(1521..1763)
                     /locus_tag="HJX07_28550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05210.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1901..2206)
                     /locus_tag="HJX07_28555"
     CDS             complement(1901..2206)
                     /locus_tag="HJX07_28555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05211.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGDLSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(2263..2640)
                     /locus_tag="HJX07_28560"
     CDS             complement(2263..2640)
                     /locus_tag="HJX07_28560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05212.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     Typing          complement(39..404)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
     Typing          complement(2784..2922)
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.915"
                     /coverage="94.0"
                     /evalue="4.48e-33"
ORIGIN
        1 cctcacactt caacacttct ttgttctgcg aatccgttac cagtggctgc tgccagtggc
       61 gataagtcgt gtctttccgg attggactca agacgatagt taccggataa ggcgcagcag
      121 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
      181 ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggagtga caccggtaaa
      241 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
      301 ttatagtcct gtcgggtttc gccaccactg atttgagcgt ccgtttctgc gatgcttgtc
      361 aggggggcgg agcctatgga aaaacggcaa cgccgtggcc tttccttttt ttcctccttt
      421 gaccgctcgg ttttctcgag cttttctatg catgctcctg ccgctcgccg cagccgaacg
      481 accggagcgt agcgactgag tgagcgagga agcggaaaag agacaggttt tacactgagc
      541 actgacgctc tgaggccctt tatgacgaaa caacagaaaa cggcgctgaa catggctaaa
      601 ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttga tcttgatgct
      661 gaggccgacc tttgtgagaa actccacgat gatgcagagc atcttttccg tacactctcc
      721 tcgagactgg attcattaca ggatggtaac tgatggccag agtgtctatt agtgaagctg
      781 cacggcttgt taaggttagt cgtcccacta tttacaagat gattaacagc gggaagttaa
      841 gttatacctc tgttgtaaag cacgggaagg atattaaagt tattgatgta tccgagctta
      901 ttagggtgtt tggttctctt gatggtgtaa ctgatactgt aaaatatgat gtaaaaagtg
      961 acgcagaatc tacaggtatt aacagcgttg gtttacatga tttacaacat agaatcgctt
     1021 tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa ggctcgagat gagcatattg
     1081 attcccttcg tcaggctatg cagcttctgg agcacaagca cgaaccttct tcaccatccc
     1141 attccccgtg gtggaaattc tggaaaaaat cctgatcctg ccatgcttga gttttcctct
     1201 ccggccatcc cggagagttt ctgaaccttc ccaaaaatcg ccagatctct gaacgctcct
     1261 caccactaaa aatacccaca ccccacgcaa aattaaaatt ttgcagattc attatttaca
     1321 acaatgggtt aatgaaaaat aaatgtgcgg tgatgtgtta caaagtgcag atccaagtcg
     1381 tggacgactc tatggcgaat cgctgacgaa ttgcccacga ttcatatgtg aatcacgctt
     1441 tattatcata ttgttatgat gaatcgactc ttgattcaat tcatcacttt gggtgttttt
     1501 tgctcatggt gactcttgat tcaattcacg attcgaatcg ccggtgaatc gtgtggtgag
     1561 tcgtccggcg acttgccata acgtgattgc cggccgtcga cgaacccgat tcctgaaggt
     1621 gctgaaaacc ggttttccgg ctgcgccggc aacgcttcac ggccacgcaa taaattgcgt
     1681 ccccgttcac cgttttcgga ggagaagaac gccggaaaca ggacttcccc tggctgaatg
     1741 gtgtgaattt ccgcgctatg cacttgcgcg catactcatg catgccgtaa aaacagagcc
     1801 tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt tttaccggtt tcccgtcaga
     1861 aacgccctga gggcctctca ggcggtgctg ataacggatg ttatggtaaa tgttggatag
     1921 caaggactat cagtccggca atgctacata tcattgctaa tggatttacg agcaaaatgc
     1981 cgaatgttgt cgcagataat ccgcgaacaa tgatattggc aagaagtccg ccaacaatga
     2041 gggataaaaa ggtataacca gcaactttca gtaacgacag atctccccat atcacctcta
     2101 ctatcggata gataaaaaca ccaacgccag caggaagcgt agtaaccatc aacagtaaat
     2161 ggcttttagt gggaccgtac tctctgacat ttccaacttt catcatgtgt gtgatgaatg
     2221 acgatatcca ccataggatc atgatcataa ggaaattcat aattatttac tctcgatgcg
     2281 tagatcaggt gctttctgta gatttggtcg tacatactcg ctgtctcttt cgtcaagaca
     2341 acggaagtag agtgttactg tggtatctat ccccattctt gtgttagcca cgttttcgtt
     2401 agtgagcagg aattttttac cgaggctatt acatttctca gaacctgttt taaaggcact
     2461 ttgacgtgct gatgccgcat ctctaaaacc atcagcagtt gctgaaaccg tatatgtatc
     2521 cctacctaac tcaagaacgt tgctggtaga aacacaacca actgtaagca gcgatattga
     2581 taaaatacct aacctataaa tcattgattt tattacccaa gttgggtgat tgtggatcat
     2641 gattatatct gtttttttta ttattaaaat caaggggttc aataattttc ttcaaagaac
     2701 tggtgtcgtg gagtggtgct ggtttccctt tgggggggag agggatggaa taggatcctc
     2761 ttgagatcct ttctgttgcg tcgtaatctc ttgccctgga aacgaaaaaa ccgcccagca
     2821 aggcggtttt ttcgaaggtt cttagagcag caactctttg aaccgaggta actggctttc
     2881 gcagagcaca gaaaccaaat tagttattac agtgtagctg tacttca
//
