LOCUS       CP052419                4654 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0189 plasmid pC16KP0189-5,
            complete sequence.
ACCESSION   CP052419
VERSION     CP052419.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609895
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:08:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,713
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 70 of 131
            Pseudo Genes (incomplete)         :: 63 of 131
            Pseudo Genes (internal stop)      :: 18 of 131
            Pseudo Genes (multiple problems)  :: 19 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0189"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0189-5"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4312..4654,1..224))
                     /locus_tag="HJX07_28490"
     CDS             complement(join(4312..4654,1..224))
                     /locus_tag="HJX07_28490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05206.1"
                     /translation="MNMKTLKDIIEKQKKEEEEQKFDPAEQIEEYISLVEKLYTITLES
                     IDELIKDGLIKQSRKKISINEESLGDYEIDSLILTINSSKIKFEPVGTMLIGSKGRVDV
                     TGPFGKEKFILIRKGVKSPSDLIKVRVNVVGAYKPEKDKTDEDVKKPSIDDWEWKTMPS
                     DSRWVKFDDVNSETITNIIMRMING"
                     /name="hypothetical protein"
     gene            complement(491..604)
                     /locus_tag="HJX07_28495"
     CDS             complement(491..604)
                     /locus_tag="HJX07_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJL05200.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(629..1063)
                     /locus_tag="HJX07_28500"
                     /pseudo=""
     CDS             complement(629..1063)
                     /locus_tag="HJX07_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            1020..1211
                     /locus_tag="HJX07_28505"
     CDS             1020..1211
                     /locus_tag="HJX07_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL05201.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1211..1612
                     /locus_tag="HJX07_28510"
     CDS             1211..1612
                     /locus_tag="HJX07_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJL05202.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMLDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2396..2471)
                     /locus_tag="HJX07_28515"
     tRNA            complement(2396..2471)
                     /locus_tag="HJX07_28515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2436..2438),aa:Arg,seq:tct)
                     /name="tRNA-Arg"
     gene            complement(2652..2894)
                     /locus_tag="HJX07_28520"
     CDS             complement(2652..2894)
                     /locus_tag="HJX07_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05203.1"
                     /translation="MSKESELEFRTKELERQMKGVQRRIEVVNAKYDSQTKKQERRIRD
                     LEIKTAVQSGVTQREVANIYELSPGRVNQIIKKVG"
                     /name="hypothetical protein"
     gene            3284..3679
                     /locus_tag="HJX07_28525"
     CDS             3284..3679
                     /locus_tag="HJX07_28525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05204.1"
                     /translation="MNANEVKDILEHSNHDDWIVDDETGSFTYKNDLNLHIKRADYDNY
                     NSFSEPWAVSHPNPNACSVQYTVNYGSSFVDRKTLVSVDGHRATLPMPASANDLTVTSA
                     DVNFAKIVDTGERVDEYILRSGLKVQP"
                     /name="hypothetical protein"
     gene            complement(3768..4319)
                     /locus_tag="HJX07_28530"
     CDS             complement(3768..4319)
                     /locus_tag="HJX07_28530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05205.1"
                     /translation="MARVHAIKRSGLHIDIDYVVHKKIALDRAVILRYSTKNPDYHADF
                     VGLSEPEIEREKDLILKENDTTHCLLLLTAIEAIFRLDADQKYCNKKRDPLSRKIKEIY
                     DKTKGEKIKLDDDLINTRMNEDDARLKKDYNDLKTCFKFRHWLAHGRYWHPKRIKNFYF
                     DSILIVIDSILNSTKYNFEV"
                     /name="hypothetical protein"
     Typing          complement(492..899)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.866"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcgatttcat aatcacctag agactcttca tttattgata ttttttttct tgattgtttt
       61 atcaatccat ctttgattag ttcgtcaata gattctaatg taatagtata taatttttca
      121 acaaggctta tgtattcttc tatttgttcc gctgggtcga atttttgttc ttcttcttct
      181 tttttttgtt tttcaattat atctttcagc gttttcatgt tcattttttg taacctctag
      241 atcggaatta ttttacgatt ctagcctttg ctttggtgtc gagcaatgtg ttgttcttga
      301 cgcttaactt gctcggaggt attgtcgctt ctgaaacgaa aaaaccgcct tggcgggcgg
      361 ttttttcgaa ggttcataga gttggcgctc gttgaaccga ggtaactggc ttggaggagc
      421 gcagtcacca aaacttgttc tttcagttta gccttaactg gcgcataact tcaagacaaa
      481 ctcctctaaa tcagttacca gtggctgctg ccagtggcgc ttttgcatgt ctttccgggt
      541 tggactcaag atgatagtta ccggataaga cgcagcagtc ggactgaacg gggggttcgt
      601 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
      661 gataaacgcg gccataacag cggaatgaca ctggtaaacc gaaaggcagg aacaggagag
      721 cgcacgaggg agccatcagg gggaaacgcc tggtatcttt aaagtcctgt cgggtttcgc
      781 caccactgat ttgagtgtca ttctctgtga tgcttgtcag gggggcggag cctatggaaa
      841 aacggccgcc gtgcggcatt tccttttccc cgtcacgcta ccgctcggtt ttctcgagct
      901 cttgtacgca tactcctgcc gctcgccgca gccgaacggc aggagcgtag cgactgagtg
      961 agcgaggaag cggaaaagag aacataccta cactgagcac tgacgctctg aggcctctta
     1021 tgtctaaaca acagaaaaca gcgctgaaca tggctaaatt catccagaat cagtctctgc
     1081 tcctgctgga gaaacttaac gagcttgatc tcgatgtcga agccgacctt tgtgagaaac
     1141 tccacgatga tgcggagcat cttttccgta cactgtcctc gagactggat gcattacagg
     1201 atggtaactg atggccagag tgtctattag tgaggctgcc cgactgacgg gtaagagtag
     1261 aacaacactg cacaggttaa ttaaagcagg tgacctgtcc acctgtagcg gtgaacgtaa
     1321 tgcaaaaatg ttagatactt cagagctttt acgtgtattt ggtccctttg agcaaccgaa
     1381 gggtgaacac cattccggac aggtctctga acagcgtgat acagcctctg ctgaccagag
     1441 tgaacaggtg attcgtcatc tgaaacagga gattgaacac ctgaacacct tacttttgtc
     1501 caaggattcg cacattgata gcctgaaaca agccatgctc cttcttgaaa atcaccaggc
     1561 aaaattaact gaatccccgg cgccatggtg gaaattctgg aaaaaatcct gaccccggca
     1621 tgcttcagtt ttcctctccg ggcatcccgg ggagttcctg aaccttccca aaaatcgcca
     1681 gatctctgaa cgctcctcac cactaaaaat acccacaccc cacgcaaaat taacattttg
     1741 cggatttgta tttaatatca gtagtttaag ttggtgaaat ttcgtttttt aaatttgatt
     1801 tatatagatc cgactctatg acgactcgtc atagagtccc cggcgagtcg tcatagagtc
     1861 ggtaacgact caggactata aaaacaatga gttaataacg acccgactct agacccgact
     1921 cccagtttgg ttgtttttta gacgtggcga ctctgggtac gactctagtg aagagtcgcc
     1981 ggtgaatcgt gtggtgagtc gttcggtgac ttgccataac gtgattgccg gccgtcgacg
     2041 aaccggattc ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg
     2101 ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg
     2161 acttaccctg gctgaatggc gtgaattttc gcgctatgca cttgcgtgca tactcatgca
     2221 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     2281 taccggtttc ccgtcagaaa cgccctgagg ccgttttagc ggtgcgcgta atgcgacgtt
     2341 atggtaaatg ctgtggccag aatagttgcc tcccccctaa aaggggggag gcttttggtg
     2401 gagacaatgg gatttgaacc cacaaccatc agattagaag tccgctgctc taccgttgaa
     2461 gctatatccc catctggacc caacaaagtc gggaccgtct ttgatttaac atcactgcat
     2521 gctttttgtg aagtttttta tgtgtttacc ggtcgcatca gaacagggta tgtaatgaga
     2581 ccttatggta aaaagcacgt cagtttgtat tgcgagaaaa aaccgccttt tggcggtttt
     2641 ttctctagag attagccgac ttttttaatg atctgattaa cgcgacccgg cgacagttcg
     2701 taaatgtttg caacctcacg ttgcgtaacg cctgactgga ctgctgtttt gatttccaaa
     2761 tcacgaatgc gacgttcttg cttcttggtt tggctgtcgt atttggcgtt aaccacctca
     2821 atacgtcgct ggacaccttt catttgacgt tcgagttctt ttgttctgaa ctctagttct
     2881 gattctttac tcatatgata tactcgctaa gtaaggttat tgttgggtgt cattcgaagg
     2941 tttcttcttg ccggaaggtc ttaacctatg tttgacaaat caacaatagc ctttttttac
     3001 gatcacagca acttctataa tcagtgtttc cccttatttt tctttgtttt tattttccaa
     3061 taaaaacaat aagttgcgat tggtttaatt ctgatattaa aaaggctaaa cacccctgtt
     3121 ttatgtatat gatttttaag tgaaaaaaca aatcgatgtt tagtctttat tatttgattc
     3181 tttactggaa aatttgattg cacatagaga acttttcgga gcagtgtggt gtagaactca
     3241 atcgatcttt acattctaac cattgattta tatgggggta tgcatgaatg ctaatgaagt
     3301 aaaagacatt cttgaacatt ctaatcatga tgattggatt gtggatgatg agactgggtc
     3361 gtttacatat aaaaacgact taaatttgca cattaagaga gcggactatg acaactacaa
     3421 ctcctttagt gaaccctggg ctgttagtca tccgaatccg aatgcctgtt cagtacagta
     3481 caccgtaaac tatggttcat catttgttga tagaaagact ttggtttctg tcgatggtca
     3541 ccgcgcaacc ttgcctatgc ctgcatccgc aaatgattta accgtgacat cagctgacgt
     3601 gaactttgcc aaaattgtag atactgggga gcgtgttgat gaatacattc tccgctctgg
     3661 tcttaaagtt caaccatgat ttaaatgtga gccagcaatc taaaattgct ggctcataat
     3721 gtgctgtgct taagggacgt tatggtgact tttttaatgt cactgaccta aacttcaaaa
     3781 ttgtatttgg ttgagtttaa aatggaatct atcactatta gtatagagtc gaaataaaaa
     3841 ttctttattc tctttggatg ccagtatctt ccatgagcaa gccaatgcct gaatttaaaa
     3901 catgttttta gatcgttgta gtcttttttt aatctagcat catcttcatt cattctggta
     3961 tttattaaat catcatctaa ttttattttt tctccttttg ttttatcata tatctctttt
     4021 atttttcttg ataatgggtc tcttttttta ttgcagtact tctgatctgc atctaatcga
     4081 aatattgctt caatagctgt taacaataaa aggcaatgtg ttgtgtcatt ttcttttagt
     4141 attaagtctt tttctctttc aatctccggc tcacttaagc cgacgaaatc ggcatggtaa
     4201 tctgggttct ttgttgaata ccgcagaatt acagcacgat cgagtgctat ctttttatgt
     4261 acgacatagt caatgtctat gtgtagacca gatcttttaa ttgcatgaac cctagccatt
     4321 tatcattctc ataataatat tcgtaatggt ctcggaattc acatcatcaa atttaaccca
     4381 tcgactatcg ctaggcattg ttttccattc ccaatcatca attgacggct ttttcacatc
     4441 ttcatcggtc ttatcttttt ctggtttata tgccccgact acgttaactc ttacttttat
     4501 taaatcactg gggcttttga ctccttttct aatcagaatg aatttttctt ttccaaatgg
     4561 ccctgtgacg tcaacccttc cttttgaacc tattagcatg gttcctactg gctcaaattt
     4621 tattttactt gagtttatag tgagtattag agag
//
