LOCUS       CP052418               10085 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C16KP0189 plasmid pC16KP0189-4,
            complete sequence.
ACCESSION   CP052418
VERSION     CP052418.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609895
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:08:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,713
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 70 of 131
            Pseudo Genes (incomplete)         :: 63 of 131
            Pseudo Genes (internal stop)      :: 18 of 131
            Pseudo Genes (multiple problems)  :: 19 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C16KP0189"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC16KP0189-4"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(9603..10085,1..249))
                     /locus_tag="HJX07_28430"
     CDS             complement(join(9603..10085,1..249))
                     /locus_tag="HJX07_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJL05189.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(249..2176)
                     /locus_tag="HJX07_28435"
                     /pseudo=""
     CDS             complement(249..2176)
                     /locus_tag="HJX07_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3657..3806)
                     /locus_tag="HJX07_28440"
     CDS             complement(3657..3806)
                     /locus_tag="HJX07_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJL05190.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3891..4148)
                     /locus_tag="HJX07_28445"
     CDS             complement(3891..4148)
                     /locus_tag="HJX07_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJL05191.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(4158..5555)
                     /locus_tag="HJX07_28450"
     CDS             complement(4158..5555)
                     /locus_tag="HJX07_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJL05199.1"
                     /translation="MAFGLPALATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLW
                     GIAIYGVLPSEIAKDDPKMMSKIVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKD
                     ERQHIAVVTGRPMSVPVVDAKPTKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIA
                     EKGDSRPAGFTAGGNSREAVIRFPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAW
                     DAAHPEEGLKREYDKAKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAE
                     ANKDDFVTYNPPHEYGSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALS
                     GAMESRKQKEKKAKDAENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPK
                     TPKQGGGGKRARWYGDKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPK
                     RNIKKYL"
                     /name="cloacin"
     gene            5660..6364
                     /locus_tag="HJX07_28455"
     CDS             5660..6364
                     /locus_tag="HJX07_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="QJL05192.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            7074..7493
                     /gene="exc2"
                     /locus_tag="HJX07_28460"
     CDS             7074..7493
                     /gene="exc2"
                     /locus_tag="HJX07_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJL05193.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            7799..8062
                     /locus_tag="HJX07_28465"
     CDS             7799..8062
                     /locus_tag="HJX07_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJL05194.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            8052..8351
                     /locus_tag="HJX07_28470"
     CDS             8052..8351
                     /locus_tag="HJX07_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJL05195.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8413..8790)
                     /locus_tag="HJX07_28475"
     CDS             complement(8413..8790)
                     /locus_tag="HJX07_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJL05196.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            8859..9038
                     /locus_tag="HJX07_28480"
     CDS             8859..9038
                     /locus_tag="HJX07_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL05197.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9066..9596)
                     /locus_tag="HJX07_28485"
     CDS             complement(9066..9596)
                     /locus_tag="HJX07_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL05198.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10085
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2976..3554
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..249)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.9e-36"
ORIGIN
        1 cggcgtcagc ccccacacgc tgacctgacg gccgtccacc ggatatttgg tccggaccag
       61 cagccctttc gccaccaggc tgtcgaggaa gcggtagacc ggctgcaccg ccgcaatccc
      121 cagccgccgc gcaaccagct ccgccgtgga acacagctct tcccgcaacc agcgcagggt
      181 cagccgggtt ttctccgcgt tccgcgcctg tcgtttcgcg tggctgacgt tgtacagctc
      241 cagcgccatc agtaaatccc cgcaaaatca tcatccgctt tcggcccgcc gccgtcggcc
      301 gggggccgga actccgccag gggatcggtt ttcgccagca ccgggaaacg ctgcaacggg
      361 atttcccggc gctcaacccg cagcggactg ctgaacgcca tccgggccac acccagcccg
      421 gtcatgaccg cgaacccgtc cggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc
      481 gcctccttct ggatgaagct gtccccgctg atatgctcca cgttccccgc ctcccggctg
      541 gcacggcggc gctccacgtc caccctgatt tccccggtct gccccgccgc ccactgcgcg
      601 gactccaggc tggccgcccg gtaaaaccag cggatcggcg tgttatccag taccgtggtt
      661 ttcacaaatt cagcgggcag atcctgcccg cactgcccga aatcgcccag cgactggtgc
      721 gcgagcaaca gattgcagtc ccggctgcgc agcgtcccca gtgcgttcag tacatacttc
      781 gacagcagat atttaatttc atcgagcatc acgctgacgt gtggccactc ccggaactcg
      841 tcccgcgcaa tgatgatttg cgcgcagcgg gcaaacagca ttttttgcac acggatcacc
      901 gcctcgtcgt ccatcgagcc gataacgtac aggcagccgc cgccgttgat aatccccgcc
      961 acatcgatgc cactgtccgt ctgcaggacg ctcaggtcag cgacgttttc cagcctagtg
     1021 atcaacccct tcacgtcctt tttcagggcc tccggcagcg cgtacgccgc cgccagcacc
     1081 tgctgaatgt ttgccccctg cggaaactgc tccgcaatca gcttcgccgc tttctgctcg
     1141 ctgatgcggt agtggtccgc cacgtcgccg gtctcgccca ggttaagccc ggcaatcagc
     1201 aggtttttca gcgcgtactg gtcgagatcg cggaacagat tcagctgcgc cggtttcccc
     1261 ggccgcagat ccagcagggt gaagtccgga cagtgcgccc ggaagacgtg cggcgcccat
     1321 gcgtcccctt tggggtcgaa gtacaccacc gcgtccccga aatcgcgtac gcagcgaatg
     1381 agcgcgttgg tggccatcac gctcttgccg ctcccgggca ggccgaggat ctgaatgttg
     1441 gtcttgcgcc aggtatcacg cggaaccagc accggcttgc cctgctcatc cttacccaga
     1501 aacaccgcct gctcctgccg ggcctgctcc gaggaaaaat cggccagcgc attaaccggt
     1561 accggctccc cgaaatgcag gtggcgcacg tcggtcatct ctcctgtccg ctggctgctg
     1621 cgccggctct cctgattcag tttcgcgttc accgcctcgt cagcagccgc gccaacacaa
     1681 tcccgacagc cagcccgccc gcgcaccagg caatcccgaa caggttagcc ggctgcagca
     1741 ggtaataatc tgcgtttgcg tagaggtccg gacgcaggac ggtttctccc cagctctcga
     1801 taaacacggc gacaaccagc ccccccagaa gggccagacc ggccgcgccg gtcagccagt
     1861 acgcgtagtt ccagacatgt cgacgtgccc tgaccatccg cccgggcgaa tccagcgagg
     1921 ccagcaccga aaatggcgct ggccgtttat tatcgcgctt atagcgatcg gcatactccc
     1981 gtcgctcctt atgactcagc cccacaaaac gcatccgtcc ggcccaaagt tcagccagac
     2041 cgaaaaacag gccgataact aggaaagccc cggccagacc gtaatactcc tggtacagcc
     2101 agctgaccca cggataaatc tgccgggcca gctccggaat gtgccacttc caggcaagcg
     2161 aatccgtatt aaacatgttc tctctccttc agacgatggt ttgccggcgt gatgtgcagc
     2221 ccccgctgca caagccgcgg acatgcccca aggcatggcg gcggcggtca cgtccgtggt
     2281 gattgcgttc agctttcacc acggaggcca gcctctttcc gccccatgcg gaaaaggcgc
     2341 agccccggag gaacaacgag cacgccccag cgtgtgagag gaaaaggcgg caccccagtg
     2401 cggtcttttc cggtgttccg gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc
     2461 gcaaaatcag gagcagcttt gctgcttcgc caggtgcgtc cagcaccgtg gatttttgtg
     2521 ggtggtcagg cgtgagcctg acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc
     2581 cggacggagc cagaaagagg gggagagacc gtgcgtcggt ggcgtccagc cagcgtaagc
     2641 aggggatatc cccctgaggt cagccacgcc ctcgcgggga tgacctgtat ttctccgtaa
     2701 ggagaaaaaa gcaacagaga taggttaacg taaaatgttc agcgaaaaac cgatatgatt
     2761 cacctatcct ggtaatttat tatcagggaa tataaagact taatcagggg acaatatgcc
     2821 acatcagcgc gtccggtgtc cgtcttgtag gtgccgggga aattgcgcct cctcgctcac
     2881 tcggtcgcta cgctccggcc gtgagactgc ggcgggcgct gcggacacat gcaaagttac
     2941 ccacagattc cgtgaataag caggggacta acatgtgagg caaaacggca aggccacgca
     3001 ggtggcgttt ttccataggc tccgccctcc tgactgagtt cacataaaca gacgcttttt
     3061 cggtgcatct gtgggagccg gtggctcaac catgaatcag tcaggagtgg cgaaacccga
     3121 caggacttaa agatcatcac cgtttccgat gaatcgctcc ctcttgcgct ctcctgttcc
     3181 aaccctgccg tttcccggat acctgttccg cctttctccc ttgcgggaag tgtggcgctt
     3241 tctcatagct cacactctgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct
     3301 gtaagcaaga accccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg
     3361 agtccaaccc ggaaaagcac gcaaaacgcc actggcagca gccattggta actgggagtt
     3421 cgcagaggat ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc
     3481 tacactggaa ggacagattt ggttgctgtg ctctgcgaaa gccagttacc acggttaagc
     3541 agttccccaa ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgtttac
     3601 agggcaaaag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3661 tgaaccgcga tccccgtcag ttcagaggag gacgatggtg ccaccgttcc accctgaaca
     3721 tcccggatat agtttgcctg acatgcgacc agaagaaagc ctgaaaaaat caatataaaa
     3781 agaaaaacaa cctttacctt tttcatatac cttccctgta tttaaaaacc ccggtattca
     3841 tccctgaaca ccggaagtct gtatttcaaa tcatccttga taacttttgc ttaccagtta
     3901 ccatcccggt aaacaaagga tacaaagtaa tcaaatttac taatatcgat tacattttta
     3961 aagtgtggct gtaatatcga aacccatgat ttttcaacat caaaccaacc attattaata
     4021 ttatccttta aaggcatccc cagactttca atgacagaac catcatcacc gaagtctttt
     4081 gagtattcac cacctttaaa ctcttcggtt ttcttatcaa accaatgaat atgtaattta
     4141 agccccatat ttacctctta aagatatttt ttaatgtttc gttttggatc cggcccttta
     4201 acctgcttac ccgtttttgg atcgaatgcc ccgaggtgtt cgccatcact ggcgcggtac
     4261 ccttcaagct caccgtgctg ggaatcccat tcataaattt tacgcttttt atcaccatac
     4321 catcgggccc gcttaccacc accaccttgt ttaggggttt taggtttacc ctctttcaac
     4381 tctcccaacc ccttaatatc ttcagtcttg ggatcaggaa aataatcatg gccgtaatct
     4441 ttagttccct tacgaggttt tttcttttcc tcatttaatt tattttctgc atccttcgct
     4501 tttttctcct tttgtttccg gctctccatc gccccggaaa gcgcagcctt atccctggat
     4561 aaggattcat tcatttcgtt taaagatgtc tgggcagctt ttaatttctc attctgattc
     4621 tgaatatcct tatcaagata gcgaacctga tcctgccacc cggagccata ttcatgaggc
     4681 gggttataag tcacaaaatc atctttattt gcctctgcct cttttacctt tttatcggct
     4741 tcctgaacag cagcccttgc accggggatc gccttctctg tcgatgcaat gcgactgttt
     4801 aaggtcgcaa tatttttatc ttcagcatcc agctcagctt tcgctttatc atactctctt
     4861 ttcagcccct cttccgggtg agcagcgtcc catgcctgct ggcgacgctt ttcttcctcc
     4921 agacgctgtt ttacctgtgc cggagtaaga acatccgtca ccgacacata gaccggtttc
     4981 tgtccgctct ctttcgggaa acgaataacg gcttcacggg agttaccacc ggccgtaaaa
     5041 ccagccggac gtgaatcacc tttttcagca ataatgcctt ttggcggggc tttcgcggcc
     5101 ggaacacctt taggtacgct cacctgcaga gacgggagac ccggaatcga cacactgaat
     5161 acccccggac gttttgtcgg tttcgcatcc accacaggaa cactcattgg ccggccggtg
     5221 acaaccgcaa tatgctgccg ctcgtccttc acaatatccg ccacacgttt agtgacgctg
     5281 accgtcgcct ggtccagcgg cagggagctg accggcgtct ccgttaccgt gtctgccggc
     5341 aatgacgtca cgatttttga catcattttc ggatcatctt ttgctatctc agaaggcagc
     5401 actccgtaaa tcgcaatccc ccacagacca aacttaaacg gacctttcag ggcagccagc
     5461 acatcagcaa cagcggccga caacgcatcg ccggaaacgg ataaagccag tccttcagcc
     5521 cccggagtgg ccaaggcagg cagaccgaag gccatcgcgg tggccgaaga ctgtccgcca
     5581 ccagagctcc cgctatggca ctgttgcaaa tagtcggtgg tgataaactt atcatcccct
     5641 tttgctgatg gagctgcaca tgaacccatt caaaggccgg cattttcagc gtgacatcat
     5701 tctgtgggcc gtacgctggt actgcaaata cggcatcagt taccgtgagc tgcaggagat
     5761 gctggctgaa cgcggagtga atgtcgatca ctccacgatt taccgctggg ttcagcgtta
     5821 tgcgcctgaa atggaaaaac ggctgcgctg gtactggcgt aacccttccg atctttgccc
     5881 gtggcacatg gatgaaacct acgtgaaggt caatggccgc tgggcgtatc tgtaccgggc
     5941 cgtcgacagc cggggccgca ctgtcgattt ttatctctcc tcccgtcgta acagcaaagc
     6001 tgcataccgg tttctgggta aaatcctcaa caacgtgaag aagtggcaga tcccgcgatt
     6061 catcaacacg gataaagcgc ccgcctatgg tcgcgcgctt gctctgctca aacgcgaagg
     6121 ccggtgcccg tctgacgttg aacaccgaca gattaagtac cggaacaacg tgattgaatg
     6181 cgatcatggc aaactgaaac ggataatcaa cgccacgctg ggatttaaat ccatgaagac
     6241 ggcttacgcc accatcaaag gtattgaggt gatgcgtgca ctacgcaaag gccaggcctc
     6301 agcattttat tatggtgatc ccctgggcga aatgcgcctg gtaagcagag tttttgaaat
     6361 gtaaggcctt tgaataagac aaaaggctgc ctcatcgcta actttgcaac agtgcccccg
     6421 ctatgattgc cgctgttacc gccattacca ccgggtttac tcccgccgtt accaaactcc
     6481 cccgggttat agttatgaat atccccgtta ggtgtatgcg tcgtacccca gcccggcccg
     6541 ctgtttggat ccgtaccacc acctgatgaa gggccacctt tacctgatgt accgttcaca
     6601 ttcccactgg cctgaccacc attgtgtccc agacctgaat tacccggacc tcgtccgtct
     6661 ccgccactca tatacttttc ctctttgact tttaaaacaa taaactaaaa taaaatactg
     6721 tacatataac cacttgtttt atatacagta taaaaattac gctgccttgt ttttcatgtc
     6781 aagtctgtaa atttgcgttt ttgtgaaaat gatcaaaaaa acagccactc acaccatgcg
     6841 caatcccctc cagatgcaac aaaaaccagc acaaacggcg ccgttctccg gcctcagtat
     6901 gttttactga aatcattata tgagatacca gaaagccccc tccgggacag gaaaagaaac
     6961 ggggggtctg gttcgggttg ttcggtcact gaacacggtc agaaactgat gataagatag
     7021 cctgcggata tataactcaa tgagaaaaaa gaattaaagg taaggagacg ctcatgagac
     7081 gttccggcag caaactgtgt cttatcgcca tgataacagc gacagcaact ctcactgcct
     7141 gtgctccaaa gggcagcgtg gaacagcata cccggcatta cgtatatgct tcagatgacg
     7201 gttttgatcc caatttttcc acccagaaag ctgacacaac acgaatgatg gtgccttttt
     7261 tccggcagtt ctgggatatg ggcgcaaagg ataaagcgac cggaaaatca cggagtgatg
     7321 tgcagcaacg cattcagcag tttcacagtc aggaattttt aaactcactc caaggcacaa
     7381 cacaatttgc gggtaccgat taccgcagca aagacctcac accgaaaaaa tcaaggctac
     7441 tggctgacac catttcagct gtttatctcg atggctatga aggcagacag taagtggatt
     7501 taccataacg cgtcattacg cgcaccgctc agacgggctc agggcgtttc cggcggctac
     7561 gggataaaac aggcaacaac ccccccggaa aactgccgga aacgcgcccg ataaatttta
     7621 tcctaatgca tgcctatgca cgcaactgca tggcatatgt tttgacgtaa aatggatgcc
     7681 gtaagccctt ttttaccgac tttctgcttc atcagcctga ctgacacgac catattcttt
     7741 cagaccgccc cctgctaaac ttccttcata ttgccgcatt aacccgggag gctccgcaat
     7801 gagcaacact acacactacg aaaacgccaa ctttcttcgg gaactggctg aaagcctgcc
     7861 ccgcatactg ccggaaggtg gcccggacaa agctgcgctg ttgcagcgac tggctaacga
     7921 agaactggcc caggcagagt atgaagatca ggttcgcgcc aaagtgaccg ccgcccgtgc
     7981 tgacacgcgt ccgggtatga ccacggaaca actccgccag cgcctgcatg gccgttatca
     8041 ggaactgcgc gatgccgtat gacgtggaat ggaagcaggg agccatcgag gatgtaacga
     8101 cgctgtttga ctatatcgcg gaaaattcat ccctgtggga tgcgcagaac gttaccgacc
     8161 gggttctcgc tgcggcggac aaactggccg acttcccgcg actctatgaa gtggactcac
     8221 gctatggcga ggacgttcgc cggatcagtc tgatgggaca gcatgtcctc tatgatgttg
     8281 atgaccagac cagaaaagtc cgggttctgg ccgtggtcgg ccagcgccag aatccccata
     8341 ttgttctcta ataaccggtg acggggacgc aattcattgc gtggccgtcg ccggttggac
     8401 aagcacagcg cgtcagcgtt ttccccacgg ccaccaccgg cgcggtgttc cggccggggt
     8461 ctctgctgtc tccggtttgt gttccagcag tctcatggac tgcatttcct gccgtagccc
     8521 ggccacctcc tccctgagtg cttttatctc cgcgatcaac gcgtcggagg ccattttttc
     8581 agtctcaccc gacagtctca actcgtcccg ctcgtgagtc tcatcctgtc ttaactcacc
     8641 gtacacccgg ataagctcgc tggtatccac cacccggccg ccatcagctt ccgtacggta
     8701 tgatacgcgg cctttcgcca tatcccgata cagggaactt cgggctttac ccgtcagttc
     8761 cgtcgcctgt ctcaccgtca cccacgccat tgtctcaccc cgctattttt gagacagtat
     8821 acctgacagg cgcacaccat gcccggaagg aaagaatcat gacaaaacag gaaaaaacag
     8881 ccctcaatat ggctaaattt attaaggagc agtcgctgct attgctcgat aaactgaatg
     8941 agctggatat ggataccccg gccgacatgt gcgagcggct acacgaggat gcggaaacac
     9001 tctggcaggt gatgcgggat aagctcgggg aggaataaca gcaccggcat ggccggtgct
     9061 ggcatttatt ttttgccgaa cggccaccac cggcgggcag gtttgtcctc cggcagttca
     9121 ggtaccggca cgttaaaata gcggctgtta tattcccgca tcagctccgc tttcagcttc
     9181 tcgttcaccg cgctttcccc gtgccgctca ctcaccacgt caatcatcgc gttaatctgc
     9241 tccggtgagc acttcccggc atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc
     9301 accttgcgat agaggtaatc acacagcttc attcctgtgg gtttgttccg ttcccattgc
     9361 ggatttttac cagtgaaaaa gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac
     9421 atcccgatgg atttcatcat cgccagatgc tgggcccggt cgacaaaaca gaaaacatac
     9481 gccccggtct gccaggcgag caccaccata tcttccagat gctcgctggt cggcatgatg
     9541 cagcgctcgc cgttccggtc ctccgcgtct tcaataaata ccatgtacag cgccataatc
     9601 cctcactccg ttgtcataaa tttaaagcac gcaaacaacc gttcacgatc cagctctgcc
     9661 gttttgcggg cctcgccggt ctgtgggttc acgatttcat ccaccgacag gattgcccgc
     9721 tgcaccctga cctgcgtcgc ccggtccggg cagatgtacc agatttcatc gatgccgtta
     9781 gctttgcggt tgaacagata cccggacacc acttcctgat accggcgacg gctcttgacg
     9841 gtgcgctcca cctcaaacgc caccgtttta ccgtccagct ccgccagggc gtccggcact
     9901 ttcatgcctt taagactcag acggtgcaga atgcgccagc cggtggcccc tctggcttcc
     9961 atatcaaggc gcagggattg cacggccagc cggtgcggca gatgtgctgc cgacaccctt
    10021 gacggctgaa acggaataac atccgccagc ggttcgtcct cgtcgaacga gaacgccacg
    10081 ccatg
//
