LOCUS       CP052412                2927 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C17KP0008 plasmid pC17KP0008-5,
            complete sequence.
ACCESSION   CP052412
VERSION     CP052412.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609897
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:46:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,628
            CDSs (total)                      :: 5,501
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 73 of 130
            Pseudo Genes (incomplete)         :: 65 of 130
            Pseudo Genes (internal stop)      :: 16 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C17KP0008"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC17KP0008-5"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(join(2750..2927,1..245))
                     /locus_tag="HJX09_28110"
     CDS             complement(join(2750..2927,1..245))
                     /locus_tag="HJX09_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJK36845.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(245..436)
                     /locus_tag="HJX09_28115"
     CDS             complement(245..436)
                     /locus_tag="HJX09_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK36840.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            393..827
                     /locus_tag="HJX09_28120"
     CDS             393..827
                     /locus_tag="HJX09_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="QJK36841.1"
                     /translation="MNLAMFSAVFCCFVIKGLRASVLSVKPVSFPLPRSLSRYAPVVRL
                     RRAAGACIEKLEKTERSKEEKKERPRRCRFSIGSAPLTSIAETDAQISGGETRQDYKDT
                     RRFPLAAPSCALLFLPFGLPVSLRCYGRVYLIPRLTLSSG"
                     /name="plasmid replication initiation protein"
     gene            1285..1662
                     /locus_tag="HJX09_28125"
     CDS             1285..1662
                     /locus_tag="HJX09_28125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK36842.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            1719..2024
                     /locus_tag="HJX09_28130"
     CDS             1719..2024
                     /locus_tag="HJX09_28130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK36843.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGDLSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            2162..2404
                     /locus_tag="HJX09_28135"
     CDS             2162..2404
                     /locus_tag="HJX09_28135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK36844.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          595..960
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 tgtaaaccaa cgctgttaat acctgtagat tctgcgtcac tttttacatc atattttaca
       61 gtatcagtta caccatcaag agaaccaaac accctaataa gctcggatac atcaataact
      121 ttaatatcct tcccgtgctt tacaacagag gtataactta acttcccgct gttaatcatc
      181 ttgtaaatag tgggacgact aaccttaaca agccgtgcag cttcactaat agacactctg
      241 gccatcagtt accatcctgt aatgaatcca gtctcgagga gagtgtacgg aaaagatgct
      301 ctgcatcatc gtggagtttc tcacaaaggt cggcctcagc atcaagatca agctcgttaa
      361 gcttctccag caggagcaga gactgattct ggatgaattt agccatgttc agcgccgttt
      421 tctgttgttt cgtcataaag ggcctcagag cgtcagtgct cagtgtaaaa cctgtctctt
      481 ttccgcttcc tcgctcactc agtcgctacg ctccggtcgt tcggctgcgg cgagcggcag
      541 gagcatgcat agaaaagctc gagaaaaccg agcggtcaaa ggaggaaaaa aaggaaaggc
      601 cacggcgttg ccgtttttcc ataggctccg cccccctgac aagcatcgca gaaacggacg
      661 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      721 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ctccgctgtt
      781 atggccgcgt ttatctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
      841 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctta tccggtaact
      901 atcgtcttga gtccaatccg gaaagacacg acttatcgcc actggcagca gccactggta
      961 acggattcgc agaacaaaga agtgttgaag tgtgaggtga agtacagcta cactgtaata
     1021 actaatttgg tttctgtgct ctgcgaaagc cagttacctc ggttcaaaga gttgctgctc
     1081 taagaacctt cgaaaaaacc gccttgctgg gcggtttttt cgtttccagg gcaagagatt
     1141 acgacgcaac agaaaggatc tcaagaggat cctattccat ccctctcccc cccaaaggga
     1201 aaccagcacc actccacgac accagttctt tgaagaaaat tattgaaccc cttgatttta
     1261 ataataaaaa aaacagatat aatcatgatc cacaatcacc caacttgggt aataaaatca
     1321 atgatttata ggttaggtat tttatcaata tcgctgctta cagttggttg tgtttctacc
     1381 agcaacgttc ttgagttagg tagggataca tatacggttt cagcaactgc tgatggtttt
     1441 agagatgcgg catcagcacg tcaaagtgcc tttaaaacag gttctgagaa atgtaatagc
     1501 ctcggtaaaa aattcctgct cactaacgaa aacgtggcta acacaagaat ggggatagat
     1561 accacagtaa cactctactt ccgttgtctt gacgaaagag acagcgagta tgtacgacca
     1621 aatctacaga aagcacctga tctacgcatc gagagtaaat aattatgaat ttccttatga
     1681 tcatgatcct atggtggata tcgtcattca tcacacacat gatgaaagtt ggaaatgtca
     1741 gagagtacgg tcccactaaa agccatttac tgttgatggt tactacgctt cctgctggcg
     1801 ttggtgtttt tatctatccg atagtagagg tgatatgggg agatctgtcg ttactgaaag
     1861 ttgctggtta taccttttta tccctcattg ttggcggact tcttgccaat atcattgttc
     1921 gcggattatc tgcgacaaca ttcggcattt tgctcgtaaa tccattagca atgatatgta
     1981 gcattgccgg actgatagtc cttgctatcc aacatttacc ataacatccg ttatcagcac
     2041 cgcctgagag gccctcaggg cgtttctgac gggaaaccgg taaaacaggc aacaaaccac
     2101 ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcgca
     2161 agtgcatagc gcggaaattc acaccattca gccaggggaa gtcctgtttc cggcgttctt
     2221 ctcctccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc
     2281 gcagccggaa aaccggtttt cagcaccttc aggaatcggg ttcgtcgacg gccggcaatc
     2341 acgttatggc aagtcgccgg acgactcacc acacgattca ccggcgattc gaatcgtgaa
     2401 ttgaatcaag agtcaccatg agcaaaaaac acccaaagtg atgaattgaa tcaagagtcg
     2461 attcatcata acaatatgat aataaagcgt gattcacata tgaatcgtgg gcaattcgtc
     2521 agcgattcgc catagagtcg tccacgactt ggatctgcac tttgtaacac atcaccgcac
     2581 atttattttt cattaaccca ttgttgtaaa taatgaatct gcaaaatttt aattttgcgt
     2641 ggggtgtggg tatttttagt ggtgaggagc gttcagagat ctggcgattt ttgggaaggt
     2701 tcagaaactc tccgggatgg ccggagagga aaactcaagc atggcaggat caggattttt
     2761 tccagaattt ccaccacggg gaatgggatg gtgaagaagg ttcgtgcttg tgctccagaa
     2821 gctgcatagc ctgacgaagg gaatcaatat gctcatctcg agccttaaca gcccctttaa
     2881 gaccatcgtt ttcagcttca agtaaagcga ttctatgttg taaatca
//
