LOCUS       CP052411               10071 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C17KP0008 plasmid pC17KP0008-4,
            complete sequence.
ACCESSION   CP052411
VERSION     CP052411.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609897
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:46:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,628
            CDSs (total)                      :: 5,501
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 73 of 130
            Pseudo Genes (incomplete)         :: 65 of 130
            Pseudo Genes (internal stop)      :: 16 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C17KP0008"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC17KP0008-4"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            259..522
                     /locus_tag="HJX09_28045"
     CDS             259..522
                     /locus_tag="HJX09_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJK36830.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            512..811
                     /locus_tag="HJX09_28050"
     CDS             512..811
                     /locus_tag="HJX09_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJK36831.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(873..1250)
                     /locus_tag="HJX09_28055"
     CDS             complement(873..1250)
                     /locus_tag="HJX09_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJK36832.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            1319..1498
                     /locus_tag="HJX09_28060"
     CDS             1319..1498
                     /locus_tag="HJX09_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK36833.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1526..2056)
                     /locus_tag="HJX09_28065"
     CDS             complement(1526..2056)
                     /locus_tag="HJX09_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK36834.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2063..2794)
                     /locus_tag="HJX09_28070"
     CDS             complement(2063..2794)
                     /locus_tag="HJX09_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJK36835.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(2794..4758)
                     /locus_tag="HJX09_28075"
     CDS             complement(2794..4758)
                     /locus_tag="HJX09_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJK36836.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6239..6388)
                     /locus_tag="HJX09_28080"
     CDS             complement(6239..6388)
                     /locus_tag="HJX09_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJK36837.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6473..6730)
                     /locus_tag="HJX09_28085"
     CDS             complement(6473..6730)
                     /locus_tag="HJX09_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK36838.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(6740..6862)
                     /locus_tag="HJX09_28090"
                     /pseudo=""
     CDS             complement(6740..6862)
                     /locus_tag="HJX09_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199321.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxic family protein"
                     /name="cytotoxic family protein"
     gene            6917..7614
                     /locus_tag="HJX09_28095"
                     /pseudo=""
     CDS             6917..7614
                     /locus_tag="HJX09_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(7631..9202)
                     /locus_tag="HJX09_28100"
                     /pseudo=""
     CDS             complement(7631..9202)
                     /locus_tag="HJX09_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /name="cloacin"
     gene            9605..10024
                     /gene="exc2"
                     /locus_tag="HJX09_28105"
     CDS             9605..10024
                     /gene="exc2"
                     /locus_tag="HJX09_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJK36839.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     BGC             2625..10071
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5558..6136
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2067..2794)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 agggcgtttc cggcggctac gggataaaac aggcaacaac ccccccggaa aactgccgga
       61 aacgcgcccg ataaatttta tcctaatgca tgcctatgca cgcaactgca tggcatatgt
      121 tttgacgtaa aatggatgcc gtaagccctt ttttaccgac tttctgcttc atcagcctga
      181 ctgacacgac catattcttt cagaccgccc cctgctaaac ttccttcata ttgccgcatt
      241 aacccgggag gctccgcaat gagcaacact acacactacg aaaacgccaa ctttcttcgg
      301 gaactggctg aaagcctgcc ccgcatactg ccggaaggtg gcccggacaa agctgcgctg
      361 ttgcagcgac tggctaacga agaactggcc caggcagagt atgaagatca ggttcgcgcc
      421 aaagtgaccg ccgcccgtgc tgacacgcgt ccgggtatga ccacggaaca actccgccag
      481 cgcctgcatg gccgttatca ggaactgcgc gatgccgtat gacgtggaat ggaagcaggg
      541 agccatcgag gatgtaacga cgctgtttga ctatatcgcg gaaaattcat ccctgtggga
      601 tgcgcagaac gttaccgacc gggttctcgc tgcggcggac aaactggccg acttcccgcg
      661 actctatgaa gtggactcac gctatggcga ggacgttcgc cggatcagtc tgatgggaca
      721 gcatgtcctc tatgatgttg atgaccagac cagaaaagtc cgggttctgg ccgtggtcgg
      781 ccagcgccag aatccccata ttgttctcta ataaccggtg acggggacgc aattcattgc
      841 gtggccgtcg ccggttggac aagcacagcg cgtcagcgtt ttccccacgg ccaccaccgg
      901 cgcggtgttc cggccggggt ctctgctgtc tccggtttgt gttccagcag tctcatggac
      961 tgcatttcct gccgtagccc ggccacctcc tccctgagtg cttttatctc cgcgatcaac
     1021 gcgtcggagg ccattttttc agtctcaccc gacagtctca actcgtcccg ctcgtgagtc
     1081 tcatcctgtc ttaactcacc gtacacccgg ataagctcgc tggtatccac cacccggccg
     1141 ccatcagctt ccgtacggta tgatacgcgg cctttcgcca tatcccgata cagggaactt
     1201 cgggctttac ccgtcagttc cgtcgcctgt ctcaccgtca cccacgccat tgtctcaccc
     1261 cgctattttt gagacagtat acctgacagg cgcacaccat gcccggaagg aaagaatcat
     1321 gacaaaacag gaaaaaacag ccctcaatat ggctaaattt attaaggagc agtcgctgct
     1381 attgctcgat aaactgaatg agctggatat ggataccccg gccgacatgt gcgagcggct
     1441 acacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg aggaataaca
     1501 gcaccggcat ggccggtgct ggcatttatt ttttgccgaa cggccaccac cggcgggcag
     1561 gtttgtcctc cggcagttca ggtaccggca cgttaaaata gcggctgtta tattcccgca
     1621 tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctca ctcaccacgt
     1681 caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc tgcacaccgt
     1741 tctcgatggc gaaccgctcc accttgcgat agaggtaatc acacagcttc attcctgtgg
     1801 gtttgttccg ttcccattgc ggatttttac cagtgaaaaa gtacgtggct ttttcgtcgc
     1861 tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc tgggcccggt
     1921 cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccata tcttccagat
     1981 gctcgctggt cggcatgatg cagcgctcgc cgttccggtc ctccgcgtct tcaataaata
     2041 ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac gcaaacaacc
     2101 gttcacgatc cagctctgcc gttttgcggg cctcgccggt ctgtgggttc acgatttcat
     2161 ccaccgacag gattgcccgc tgcaccctga cctgcgtcgc ccggtccggg cagatgtacc
     2221 agatttcatc gatgccgtta gctttgcggt tgaacagata cccggacacc acttcctgat
     2281 accggcgacg gctcttgacg gtgcgctcca cctcaaacgc caccgtttta ccgtccagct
     2341 ccgccagggc gtccggcact ttcatgcctt taagactcag acggtgcaga atgcgccagc
     2401 cggtggcccc tctggcttcc atatcaaggc gcagggattg cacggccagc cggtgcggca
     2461 gatgtgctgc cgacaccctt gacggctgaa acggaataac atccgccagc ggttcgtcct
     2521 cgtcgaacga gaacgccacg ccatgcggcg tcagccccca cacgctgacc tgacggccgt
     2581 ccaccggata tttggtccgg accagcagcc ctttcgccac caggctgtcg aggaagcggt
     2641 agaccggctg caccgccgca atccccagcc gccgcgcaac cagctccgcc gtggaacaca
     2701 gctcttcccg caaccagcgc agggtcagcc gggttttctc cgcgttccgc gcctgtcgtt
     2761 tcgcgtggct gacgttgtac agctccagcg ccatcagtaa atccccgcaa aatcatcatc
     2821 cgctttcggc ccgccgccgt cggccggggg ccggaactcc gccaggggat cggttttcgc
     2881 cagcaccggg aaacgctgca acgggatttc ccggcgctca acccgcagcg gactgctgaa
     2941 cgccatccgg gccacaccca gcccggtcat gaccgcgaac ccgtccggca ggtgttgcag
     3001 ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtccc cgctgatatg
     3061 ctccacgttc cccgcctccc ggctggcacg gcggcgctcc acgtccaccc tgatttcccc
     3121 ggtctgcccc gccgcccact gcgcggactc caggctggcc gcccggtaaa accagcggat
     3181 cggcgtgtta tccagtaccg tggttttcac aaattcagcg ggcagatcct gcccgcactg
     3241 cccgaaatcg cccagcgact ggtgcgcgag caacagattg cagtcccggc tgcgcagcgt
     3301 ccccagtgcg ttcagtacat acttcgacag cagatattta atttcatcga gcatcacgct
     3361 gacgtgtggc cactcccgga actcgtcccg cgcaatgatg atttgcgcgc agcgggcaaa
     3421 cagcattttt tgcacacgga tcaccgcctc gtcgtccatc gagccgataa cgtacaggca
     3481 gccgccgccg ttgataatcc ccgccacatc gatgccactg tccgtctgca ggacgctcag
     3541 gtcagcgacg ttttccagcc tagtgatcaa ccccttcacg tcctttttca gggcctccgg
     3601 cagcgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggaa actgctccgc
     3661 aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt cgccggtctc
     3721 gcccaggtta agcccggcaa tcagcaggtt tttcagcgcg tactggtcga gatcgcggaa
     3781 cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt ccggacagtg
     3841 cgcccggaag acgtgcggcg cccatgcgtc ccctttgggg tcgaagtaca ccaccgcgtc
     3901 cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcacgctct tgccgctccc
     3961 gggcaggccg aggatctgaa tgttggtctt gcgccaggta tcacgcggaa ccagcaccgg
     4021 cttgccctgc tcatccttac ccagaaacac cgcctgctcc tgccgggcct gctccgagga
     4081 aaaatcggcc agcgcattaa ccggtaccgg ctccccgaaa tgcaggtggc gcacgtcggt
     4141 catctctcct gtccgctggc tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc
     4201 ctcgtcagca gccgcaaaat gaccgtcaag cagcaggaac gccagcagcc gcgccaacac
     4261 aatcccgaca gccagcccgc ccgcgcacca ggcaatcccg aacaggttag ccggctgcag
     4321 caggtaataa tctgcgtttg cgtagaggtc cggacgcagg acggtttctc cccagctctc
     4381 gataaacacg gcgacaacca gcccccccag aagggccaga ccggccgcgc cggtcagcca
     4441 gtacgcgtag ttccagacat gtcgacgtgc cctgaccatc cgcccgggcg aatccagcga
     4501 ggccagcacc gaaaatggcg ctggccgttt attatcgcgc ttatagcgat cggcatactc
     4561 ccgtcgctcc ttatgactca gccccacaaa acgcatccgt ccggcccaaa gttcagccag
     4621 accgaaaaac aggccgataa ctaggaaagc cccggccaga ccgtaatact cctggtacag
     4681 ccagctgacc cacggataaa tctgccgggc cagctccgga atgtgccact tccaggcaag
     4741 cgaatccgta ttaaacatgt tctctctcct tcagacgatg gtttgccggc gtgatgtgca
     4801 gcccccgctg cacaagccgc ggacatgccc caaggcatgg cggcggcggt cacgtccgtg
     4861 gtgattgcgt tcagctttca ccacggaggc cagcctcttt ccgccccatg cggaaaaggc
     4921 gcagccccgg aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag
     4981 tgcggtcttt tccggtgttc cggcccccgt cagagttcgc catagcgagc ggctgaccac
     5041 ccgcaaaatc aggagcagct ttgctgcttc gccaggtgcg tccagcaccg tggatttttg
     5101 tgggtggtca ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca
     5161 gccggacgga gccagaaaga gggggagaga ccgtgcgtcg gtggcgtcca gccagcgtaa
     5221 gcaggggata tccccctgag gtcagccacg ccctcgcggg gatgacctgt atttctccgt
     5281 aaggagaaaa aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgatatga
     5341 ttcacctatc ctggtaattt attatcaggg aatataaaga cttaatcagg ggacaatatg
     5401 ccacatcagc gcgtccggtg tccgtcttgt aggtgccggg gaaattgcgc ctcctcgctc
     5461 actcggtcgc tacgctccgg ccgtgagact gcggcgggcg ctgcggacac atgcaaagtt
     5521 acccacagat tccgtgaata agcaggggac taacatgtga ggcaaaacgg caaggccacg
     5581 caggtggcgt ttttccatag gctccgccct cctgactgag ttcacataaa cagacgcttt
     5641 ttcggtgcat ctgtgggagc cggtggctca accatgaatc agtcaggagt ggcgaaaccc
     5701 gacaggactt aaagatcatc accgtttccg atgaatcgct ccctcttgcg ctctcctgtt
     5761 ccaaccctgc cgtttcccgg atacctgttc cgcctttctc ccttgcggga agtgtggcgc
     5821 tttctcatag ctcacactct ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg
     5881 ctgtaagcaa gaaccccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact
     5941 tgagtccaac ccggaaaagc acgcaaaacg ccactggcag cagccattgg taactgggag
     6001 ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg
     6061 gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta ccacggttaa
     6121 gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt tttttcgttt
     6181 acagggcaaa agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6241 actgaaccgc gatccccgtc agttcagagg aggacgatgg tgccaccgtt ccaccctgaa
     6301 catcccggat atagtttgcc tgacatgcga ccagaagaaa gcctgaaaaa atcaatataa
     6361 aaagaaaaac aacctttacc tttttcatat accttccctg tatttaaaaa ccccggtatt
     6421 catccctgaa caccggaagt ctgtatttca aatcatcctt gataactttt gcttaccagt
     6481 taccatcccg gtaaacaaag gatacaaagt aatcaaattt actaatatcg attacatttt
     6541 taaagtgtgg ctgtaatatc gaaacccatg atttttcaac atcaaaccaa ccattattaa
     6601 tattatcctt taaaggcatc cccagacttt caatgacaga accatcatca ccgaagtctt
     6661 ttgagtattc accaccttta aactcttcgg ttttcttatc aaaccaatga atatgtaatt
     6721 taagccccat atttacctct taaagatatt ttttaatgtt tcgttttgga tccggccctt
     6781 taacctgctt acccgttttt ggatcgaatg ccccgaggtg ttcgccatca ctggcgcggt
     6841 acccttcaag ctcaccgtgc tggtgatgct accaacttgc tgatttagtg tataatggtg
     6901 tttttgaggt gctcccgtgg cttcagtctc cgtctgctgt ccctcctgtt ccgctactga
     6961 aggcgtgatg cgtaatggca aaagtactgc cgggcatcaa cgttatctct gctctcactg
     7021 ccgtaaaaca tggcagctca ccttcactta tgccgcttct cagcccggta cacaccagaa
     7081 aatcattgat atggctatga acggcgtcgg ttgccgtgcc accgcacgac taatgggcgt
     7141 gggcctcaac accattttgc gccatttaaa aaactcaggc cgcagtcagt aacctcccgg
     7201 atacagccgg gcagtgatgt cattgtctgt gcggagatgg acgagcaatg gggctacgtc
     7261 ggtgcaaaat cacgtcagcg ctggctgttt tacgcgtatg acaggatacg cagggcggtg
     7321 gtagcccacg ttttcggtga acgcactatg gccacactgg agcgtcttct gagcctgctg
     7381 tcagtttttg acgtggtggt atggatgacg gacggctggc cgatgtatga gtcacgtctg
     7441 aagggaaaac tgcacgtcat cagtaagcgt tacacgcagc gaattgagcg acacaacctg
     7501 aatctgagac agcatctggc aaggctgggc aggaagtccc tgtcgttctc aaaatcggtg
     7561 gagctgcatg acaaagtcat cgggcactat ctgaacataa aacactatca gtaaattgga
     7621 gtcattaccc accgtgctgg gaatcccatt cataaatttt acgcttttta tcaccatacc
     7681 atcgggcccg cttaccacca ccaccttgtt taggggtttt aggtttaccc tctttcaact
     7741 ctcccaaccc cttaatatct tcagtcttgg gatcaggaaa ataatcatgg ccgtaatctt
     7801 tagttccctt acgaggtttt ttcttttcct catttaattt attttctgca tccttcgctt
     7861 ttttctcctt ttgtttccgg ctctccatcg ccccggaaag cgcagcctta tccctggata
     7921 aggattcatt catttcgttt aaagatgtct gggcagcttt taatttctca ttctgattct
     7981 gaatatcctt atcaagatag cgaacctgat cctgccaccc ggagccatat tcatgaggcg
     8041 ggttataagt cacaaaatca tctttatttg cctctgcctc ttttaccttt ttatcggctt
     8101 cctgaacagc agcccttgca ccggggatcg ccttctctgt cgatgcaatg cgactgttta
     8161 aggtcgcaat atttttatct tcagcatcca gctcagcttt cgctttatca tactctcttt
     8221 tcagcccctc ttccgggtga gcagcgtccc atgcctgctg gcgacgcttt tcttcctcca
     8281 gacgctgttt tacctgtgcc ggagtaagaa catccgtcac cgacacatag accggtttct
     8341 gtccgctctc tttcgggaaa cgaataacgg cttcacggga gttaccaccg gccgtaaaac
     8401 cagccggacg tgaatcacct ttttcagcaa taatgccttt tggcggggct ttcgcggccg
     8461 gaacaccttt aggtacgctc acctgcagag acgggagacc cggaatcgac acactgaata
     8521 cccccggacg ttttgtcggt ttcgcatcca ccacaggaac actcattggc cggccggtga
     8581 caaccgcaat atgctgccgc tcgtccttca caatatccgc cacacgttta gtgacgctga
     8641 ccgtcgcctg gtccagcggc agggagctga ccggcgtctc cgttaccgtg tctgccggca
     8701 atgacgtcac gatttttgac atcattttcg gatcatcttt tgctatctca gaaggcagca
     8761 ctccgtaaat cgcaatcccc cacagaccaa acttaaacgg acctttcagg gcagccagca
     8821 catcagcaac agcggccgac aacgcatcgc cggaaacgga taaagccagt ccttcagccc
     8881 ccggagtggc caaggcaggc agaccgaagg ccatcgcggt ggccgaagac tgtccgccac
     8941 cagagctccc gctatgattg ccgctgttac cgccattacc accgggttta ctcccgccgt
     9001 taccaaactc ccccgggtta tagttatgaa tatccccgtt aggtgtatgc gtcgtacccc
     9061 agcccggccc gctgtttgga tccgtaccac cacctgatga agggccacct ttacctgatg
     9121 taccgttcac attcccactg gcctgaccac cattgtgtcc cagacctgaa ttacccggac
     9181 ctcgtccgtc tccgccactc atatactttt cctctttgac ttttaaaaca ataaactaaa
     9241 ataaaatact gtacatataa ccacttgttt tatatacagt ataaaaatta cgctgccttg
     9301 tttttcatgt caagtctgta aatttgcgtt tttgtgaaaa tgatcaaaaa aacagccact
     9361 cacaccatgc gcaatcccct ccagatgcaa caaaaaccag cacaaacggc gccgttctcc
     9421 ggcctcagta tgttttactg aaatcattat atgagatacc agaaagcccc ctccgggaca
     9481 ggaaaagaaa cggggggtct ggttcgggtt gttcggtcac tgaacacggt cagaaactga
     9541 tgataagata gcctgcggat atataactca atgagaaaaa agaattaaag gtaaggagac
     9601 gctcatgaga cgttccggca gcaaactgtg tcttatcgcc atgataacag cgacagcaac
     9661 tctcactgcc tgtgctccaa agggcagcgt ggaacagcat acccggcatt acgtatatgc
     9721 ttcagatgac ggttttgatc ccaatttttc cacccagaaa gctgacacaa cacgaatgat
     9781 ggtgcctttt ttccggcagt tctgggatat gggcgcaaag gataaagcga ccggaaaatc
     9841 acggagtgat gtgcagcaac gcattcagca gtttcacagt caggaatttt taaactcact
     9901 ccaaggcaca acacaatttg cgggtaccga ttaccgcagc aaagacctca caccgaaaaa
     9961 atcaaggcta ctggctgaca ccatttcagc tgtttatctc gatggctatg aaggcagaca
    10021 gtaagtggat ttaccataac gcgtcattac gcgcaccgct cagacgggct c
//
