LOCUS       CP052406                6222 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C17KP0020 plasmid pC17KP0020-2,
            complete sequence.
ACCESSION   CP052406
VERSION     CP052406.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609899
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:40:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,409
            CDSs (with protein)               :: 5,409
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 59 of 129
            Pseudo Genes (incomplete)         :: 64 of 129
            Pseudo Genes (internal stop)      :: 26 of 129
            Pseudo Genes (multiple problems)  :: 18 of 129
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C17KP0020"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC17KP0020-2"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            19..558
                     /locus_tag="HJX11_28290"
     CDS             19..558
                     /locus_tag="HJX11_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="QJL27006.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDVFPG
                     DRRSRLISSMTSVAPA"
                     /name="IS91 family transposase"
     gene            complement(530..1366)
                     /locus_tag="HJX11_28295"
     CDS             complement(530..1366)
                     /locus_tag="HJX11_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="QJL27007.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(1366..2169)
                     /gene="aph(3'')-Ib"
                     /locus_tag="HJX11_28300"
     ARG             complement(1370..2169)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2230..3045)
                     /gene="sul2"
                     /locus_tag="HJX11_28305"
     ARG             complement(2234..3045)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3505..3663)
                     /locus_tag="HJX11_28310"
     CDS             complement(3505..3663)
                     /locus_tag="HJX11_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJL27010.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="replication initiation protein"
     gene            complement(3705..4109)
                     /locus_tag="HJX11_28315"
     CDS             complement(3705..4109)
                     /locus_tag="HJX11_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="QJL27011.1"
                     /translation="MLHVAGEKRQHRRVSRICDTGDIPLPRSLTRYARSFGCGERYRLT
                     DGDGNFLEDTRKTLSKEEIRPRRSRFSIGSAPLTSIAKSDAQISGGETRQDYKDPRRFP
                     LVAPSCALLFLPFGLPVSFRCYGRGFSFHA"
                     /name="plasmid replication initiation protein"
     gene            4234..4383
                     /locus_tag="HJX11_28320"
     CDS             4234..4383
                     /locus_tag="HJX11_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL27012.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(5150..5590)
                     /locus_tag="HJX11_28325"
     CDS             complement(5150..5590)
                     /locus_tag="HJX11_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJL27013.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            5808..6197
                     /locus_tag="HJX11_28330"
     CDS             5808..6197
                     /locus_tag="HJX11_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL27014.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             complement(534..1366)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4026..4199
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(3497..4102)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 attgcaagga cgcggaacat gcctcatgtg gcggccagga cggccagccg ggatcgggat
       61 actggtcgtt accagagcca ccgacccgag caaacccttc tctatcagat cgttgacgag
      121 tattacccgg cattcgctgc gcttatggca gagcagggaa aggaattgcc gggctatgtg
      181 caacgggaat ttgaagaatt tctccaatgc gggcggctgg agcatggctt tctacgggtt
      241 cgctgcgagt cttgccacgc cgagcacctg gtcgctttca gctgtaagcg tcgcggtttc
      301 tgcccgagct gtggggcgcg gcggatggcc gaaagtgccg ccttgctggt tgatgaagta
      361 ctgcctgaac aacccatgcg tcagtgggtg ttgagcttcc cgtttcagct gcgtttcctg
      421 tttggggtcg tttgcgggaa ggggcggaat cctacgctaa ggctttggcc agcgatattc
      481 tccggtgaga ttgatgtgtt cccaggggat aggagaagtc gcttgatatc tagtatgacg
      541 tctgtcgcac ctgcttgatc gcggccgcga tagctagatc gcgttgctcc tcttctccat
      601 ccgcgttcca agctgcggaa aggcacccat aagcgtacgc ctggtcgagc aggcgacgcg
      661 gatcgacgtc cagcgcacga gagaatgcgt ccgccatctg tgcaatgcgt ctaggatcga
      721 gacaaaggtc gtctctgtca gccggatcgt agaacatatt ggcggcgcca aagcccactt
      781 caccgaccag accgacggga tctatcacca gccagccgcg actggagaac atgatgtttt
      841 catgatgcag atcgccatgt agcccacgca gttccgaggc attgctcatc atttgatcgg
      901 ctataatcgc cgcgtggacg tagtcagttt gacaacctgc gttttgatca tcgcgcgccc
      961 gctgaaacaa agctgcaaag cgatcccgga tcgggagaag ggcagaaggc aggggttcct
     1021 cagatgcggc atacagcttc gccattagtt ccgctgcaat ttcggtcgcc tggtagtcgc
     1081 cgtgctcggc aacgatgtga gagagcattc gctccccggc atattcgagc aacatcagat
     1141 tgttctcacg accgagcaac cggactgctc ccctcccatt gcgccatacc agatagtcgg
     1201 ccccgcgcag ttcatcagca atgtcttcta taggtttcaa tcccttgacg attgcaggag
     1261 tcccgtctgg caatgaaact ttccaaacga ggctggaaaa ggtgtccgca atgagaacag
     1321 gttgcgaaac gtgccaatga gcaggaaaaa caggcggcat gaacatcaac cccaagtcag
     1381 agggtccaat cgcagataga aggcaaggcg ttcgcggtcg ggggcttcga tccccaatac
     1441 attgaatagg acagcgaagg cgcgctctgc ttcatctggc gctgcccagt tctcttcggc
     1501 gttagcaatc atgagtgcca aatcggcata gcgatctgct gttccgagcc gcccaaggtc
     1561 gatcagaccc gtgcattgaa gagttttagg gtccaccatg aagttcggca tgcagggatc
     1621 accatggcaa acaaccatat cggtgcgctc ttggtcgagc cgcaccggta gctctcgttc
     1681 gacacgagcc aaaagatcga gctgcggcgt actcttgtcc tcgtccggta agaagtcggg
     1741 attgacggca ttgcgggaca ccacatcaac ggcgcgtccg aacattcgcg acagcctgcg
     1801 ctcaaacgga cattgatcaa ccgataggct gtgaacagcg ccaagttgct gccccattga
     1861 cggccacgct ttgagcaaat ccgctccaga cagatcagcc gccggtactc ccggaattgc
     1921 cgttatcacc aagcatgcac cctcctgttc ctcctgccag ttgatgacct cggggcaagc
     1981 cacacctcga cctttgagcc aaatgaggcg gtcacgctct ccagcgagct caccgcggcg
     2041 ggaagcaggt gcgattttcg cgaaggcatg cccgtcacca cgtcgaaaaa caaaatcacc
     2101 agattctccg cctctgacag gcaaccagtc agaatgcgat tcaccaaaaa aaatattagt
     2161 tcgattcaat ggaggttcct tcagttttct gatgaagcgc gaatatagag aaatatcccg
     2221 aatgtgcagt taacgaattc ttgcggtttc tttcagcgcc gccaataccg ccagcccgtc
     2281 gcgcaagggg cgcggctcgt gtgtgcggat gaagtcagct ccacctgcgg cggcggcaag
     2341 ctctgcagcg agtgtcgcgg ccccgacatc ccccggacca cggcctgtga gcgcgcgcag
     2401 aaaggatttg cgcgaaacag acagaagcac cggcaaatcg aagcgcagcc gcaattcatc
     2461 gaaccgcgcc agcaccgaga gcgaggtttc gggagcagcc cccagaaaaa aacccatgcc
     2521 gggatcaagg acaaggcggt tgcgtttgat accggcaccc gtcagcgccg cgatgcgcgc
     2581 gtcaaagaac gccgcaatgt gatccatgat gtcgccagcg ggtgcctcgc gccgatctgc
     2641 ctgcccgtct tgcaccgaat gcataacgac gagtttggca gatgatttcg ccaattgcgg
     2701 atagaacgca gcgtctggaa aaccgcgaat atcattgaga taggccacac cacgcgacaa
     2761 ggcataggct tgcgtcgcgg gttgataact gtcgagcgag acgggaatgc catctgcctt
     2821 gagcgcgtcc agcaccggcg cgatacgcgc gatttctgtg tcggacgaaa caggcgcggc
     2881 gtcgggattg ctggatgccg gaccgaggtc gatcacatct gccccctcgg ccatcagctt
     2941 acgcgcctgc gcaatggctg cgtctggcgc cagataccgg cctccatcgg agaaactgtc
     3001 cgaggttatg ttgacgatgc cgaaaatgat gagcgattta ttcatggggg cttctataat
     3061 aataataatc gagcatgagt ctcatacgga tgctcgggtc gaaagggaat ccccaggcga
     3121 gtaacctgtt tgcggtgatc cgtttcatga tgatgtatct gtagcatttt ggaaataaat
     3181 atatcatctc tcaataagta gatatattta tcttttttgc ttctttggtg ctgcaagtca
     3241 ccaatacgag ggaaacctgt tacggagcgg tttaaccgta atcaacagtg actggtgtca
     3301 tgttcggaag attaattgat agtaatctac aaaggatctt cttgagatcc ttttcaatga
     3361 atcgtaatct cttgctctgt aaacgaaaaa accgcctggg gaggcggttt gttcgaaggt
     3421 taagtcagtt ggggaactgc ttaacccggt aacagggttc acgagaccgc cattaccaaa
     3481 actgttcttt cagtgtagcc gcagtcaggc ctctacttca agacctctta atctatcttc
     3541 tcgtgcactc ctgttactaa tggctgctgc cagtggcgtt ttgtcgtgtc tttccgggtt
     3601 ggactcaagt tgatagttac cggacaaggc gcagcggtcg gactgaacgg ggggttcgtg
     3661 catacagtcc agcttggagc gaactgcctt cacggaactg agtgtcaggc gtggaatgag
     3721 aaaccgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     3781 cacgagggag ctaccagggg gaaacgcctg ggatctttat agtcctgtcg ggtttcgcca
     3841 ccactgattt gagcgtcaga ttttgcgatg cttgtcaggg gggcggagcc tatggaaaaa
     3901 cggcttcgcc gtggccttat ctcttctttg ctaagtgtct tcctggtatc ttccaggaaa
     3961 tttccgtccc cgtccgtaag ccggtaccgc tcgccgcagc cgaacgaccg agcgtagcga
     4021 gtcagtgagc gaggaagcgg aatatctcct gtatcgcata ttctgctgac gcgccggtgc
     4081 tgcctttttt ctcctgccac atgaagcact tcactgattt tcgcaatcgt ggcaacatag
     4141 taagccagta tacactccgc tagcgctaca tgactggttc agggctgcgc cccgaaaccc
     4201 gctaacaacc tctcagctac tggatatcac cccatgccat ctgaatatga tgaacaactg
     4261 gattcgactg atacgcagct tcccggcgaa tatgaccttt ttgatttaat ggcgcaggtt
     4321 actgaagaga acctgcatgc ccctgtggat tttgggtcgc ctgtcggccg ggaagcgtta
     4381 tagctttttc gcttgcccgc gtacgtggta atactccggt attacctttt atttctttat
     4441 tttcatgtaa ttacagtgga tttttaatac ccgggtatta ctcgtgaaaa cgaaatattc
     4501 cttttttttc ttttaattca tgcagttgcg atgaaaatcg cggctgcgtt aggtgtatga
     4561 ccattttgag gttgaaaaat aatcaaccat ggcgggtgat ttacgcaggt taaggggaga
     4621 tcgtgacaaa aaaagcgttc ctcactgtat cgttcatgcc tgcatgaacg ggttattctt
     4681 cctgactaca gaattacatc gctccttcag ttcatacatc acttttacat cacttataca
     4741 tcacttttac atcacttata catcactttt acatcattcc ttcagctcat acgtcacttt
     4801 catcctctgt gccatttgta catcaccccc ttcctgaacg tgctgacagc gggttttccg
     4861 gctgcgccgg caacgcttca cggccacgca ataaattgcg tccccgttca ccgttttcgg
     4921 atgggaagaa cgccggaaac aggacttacc ccggctgaat ggtgtgaatt tccgcgctat
     4981 gcacttgtgc gcatactcat gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt
     5041 ttcgggtggt ttgttgcctg ttttaccggt ttcccgtcag aaacgccctg agggcctctc
     5101 aggcggtgct gataacagat gttatggtaa atcagagtca cctgcactgt tactctgaac
     5161 cggagcgaaa cagccgcgac cagaatcccc gctttttttc ctgtgccagc gcgtcagcca
     5221 gacggctaat ttcggcctgt agttgttccc tctcctcatt tcgcaggcgg ttttctgctt
     5281 cccgcgccat cttgtcttca agcattaggg taacagtctg ctgcagagcg tccagtttct
     5341 gctgaatgac gtctagctga gcattttccg cctggctaac gacagatttg gagtgtccct
     5401 ccttgtgcct ttttggtgtc tcagaagtgc gcagctcgcc ataggcccga atcagttcac
     5461 tggtctcgaa ctcccggcga tcattttttc ctgtgcgata gctgacaagg cctttagcca
     5521 tgtcacgata taattgcgag cgcgactttc ctgttaattt cagtgtctct tgaactgtgt
     5581 gccatgccat aaaagtgaca gtgtctcctg tgtgtaccgt attgtaacag cagaacatat
     5641 tactgtttta acacaaaagg tctgaggctg cccgtgaggc gtgaaaaata cacaaaaaac
     5701 ctgttgacgg tgtggttttg acgggcgtag tatttcgaat actgtatata aacacattgt
     5761 tataaataca gtttttgctg tttttaaaag tcaaagagga taattttatg agtacagagt
     5821 taaatagcgg tactgtcggt ggttttggtc acatgggacc aaatgacccc tacaatgatg
     5881 gtcatagcgg ctctggaagt tcgggaggta gttttaacgg tggtggtaat ggtggccatg
     5941 ataatagtag tggttcatct tatactgcat cctttggtct catcactggc cgacacccga
     6001 taactgtccc aggtacgaag aatattaatt ctgtcgatct ttctgctgtc attaataaca
     6061 ttatacatca tgaaggtgca cgcaataaca tgtacctaga tactgcgaac aatgtaagag
     6121 tgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg gctgagggta
     6181 aaagaacttt ccgctaagcg atagactgta tgtaaacaca gt
//
