LOCUS       CP052402               10045 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C17KP0039 plasmid pC17KP0039-3,
            complete sequence.
ACCESSION   CP052402
VERSION     CP052402.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609901
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:57:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,037
            CDSs (total)                      :: 5,913
            Genes (coding)                    :: 5,772
            CDSs (with protein)               :: 5,772
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 63 of 141
            Pseudo Genes (incomplete)         :: 79 of 141
            Pseudo Genes (internal stop)      :: 19 of 141
            Pseudo Genes (multiple problems)  :: 18 of 141
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C17KP0039"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC17KP0039-3"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            844..2805
                     /locus_tag="HJX13_30110"
     CDS             844..2805
                     /locus_tag="HJX13_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJL21590.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRVRRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGITWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2805..3536
                     /locus_tag="HJX13_30115"
     CDS             2805..3536
                     /locus_tag="HJX13_30115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJL21591.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQISVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            3543..4073
                     /locus_tag="HJX13_30120"
     CDS             3543..4073
                     /locus_tag="HJX13_30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL21592.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRTQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKSARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4101..4280)
                     /locus_tag="HJX13_30125"
     CDS             complement(4101..4280)
                     /locus_tag="HJX13_30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL21593.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4349..4726
                     /locus_tag="HJX13_30130"
     CDS             4349..4726
                     /locus_tag="HJX13_30130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJL21594.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(4788..5087)
                     /locus_tag="HJX13_30135"
     CDS             complement(4788..5087)
                     /locus_tag="HJX13_30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJL21595.1"
                     /translation="MPYDVEWKQGAIEDVTMLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5077..5340)
                     /locus_tag="HJX13_30140"
     CDS             complement(5077..5340)
                     /locus_tag="HJX13_30140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJL21596.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESDPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            6114..6632
                     /locus_tag="HJX13_30145"
     CDS             6114..6632
                     /locus_tag="HJX13_30145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL21597.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVAQSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            7217..8905
                     /locus_tag="HJX13_30150"
     CDS             7217..8905
                     /locus_tag="HJX13_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJL21598.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVNVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGVKDYGHDYHPAPKTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="cloacin"
     gene            8915..9172
                     /locus_tag="HJX13_30155"
     CDS             8915..9172
                     /locus_tag="HJX13_30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJL21599.1"
                     /translation="MGLKLNLTWFDKKTEEFIGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVEKSWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin"
     gene            9257..9406
                     /locus_tag="HJX13_30160"
     CDS             9257..9406
                     /locus_tag="HJX13_30160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJL21600.1"
                     /translation="MKKVKVVFLFILIFLGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2217..10045
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9510..10045)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2806..3533
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="100.0"
                     /evalue="9.930000000000001e-144"
ORIGIN
        1 tatggaaaaa cgccaccggc gcggccctgc cgttttgcct cacatgttag tcccctgctt
       61 atccacggaa tctgtgggta actttgtatg tgtccgcagc gcccgccgca gtctcacggc
      121 cggagcgtag cgaccgagtg agcgaggagg cgcaatttcc ccggcacctg taagacggtc
      181 accggacgcg ctgatgtggg atattgtccc ctgattaagt ctttctattc cctgacgatc
      241 aattatcagg ataggtgaac catgtcggtt tttcgctgaa cattttacgt taacctatct
      301 ctgttgcttt tttctccttg cggagaaatg caagtcatcc ccgctggggc gtggctgact
      361 tcagggggaa atcccctgct tacgctggct ggacgccacc gacgcacggt ctttccccct
      421 ctttctggct ctgtccggct ggacgccgga cacgccagaa attcacctgg cccccgtcag
      481 gctcacgcct gaccacccac aaaaatccac ggtgctggac gcacctgacg aagcagcaaa
      541 gctgcctctg attttgcggg tggtcagccg ctcgctatgg cgaactctga cgggggccgg
      601 aacaccggaa aagaccgcac tggggtgccg ccttttcctc tcacacgctg gggcgtgctc
      661 gttgttcctc cggggctgcg ccttttccgc atggggcgga aagaggctgg cctccgcggt
      721 gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc catgccttgg ggcatgtccg
      781 cggcttgtgc agcaggggct gcacaacacg ccggcaaacc gtcgtctgaa ggagagagat
      841 tgcatgttta atacggattc gcttgcctgg cagtggcata tcccggagct ggcccggcag
      901 atttacccgt gggtcagctg gctgtaccgg gagtattacg gtctagccgg ggcttttctg
      961 gttatcggcc tgtttttcgg tctggctgaa ctctgggccg gacggatgcg ttttgtgggg
     1021 ctgagtcata aggagcgccg ggagtatgcc gatcgctata agcgcgataa taaccggact
     1081 gcgccatttt cggtgctggc ctcgctggat tcgcccgggc ggatggtcag ggttcggcgg
     1141 catgtctgga actacgcgta ctggctgacc ggcgctgccg gtctggccct tctggggggg
     1201 cttgttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgtcc ggacctctac
     1261 gcgaacgcag attattacct gctgcagccg gctaacctgt tcgggattac ctggtgcgcg
     1321 ggcgggctgg ttgtcgggat tgtgttggcg cggctgctgg cgttcctgct gcttgacggt
     1381 cattttgcgg ccgctgacga ggcggtgaac gcgaaactga accaggagag ccggcgcagc
     1441 agccagcgga ccggtgaaat gaccgacgtc cgccacctgc atttcgggga gccggtaccg
     1501 gttaacgcgc tggccgattt ttccacggag caggcccgga aacagcaggc ggtgtttctg
     1561 ggtaaggatg agcagggcca gccggtgctg gtcccgcgtg atacctggcg caagaccaac
     1621 attcagatcc tcggcctgcc cgggagcggc aagagcgtga tggccaccaa cgcgctcatt
     1681 cgctgcgtac gcgatttcgg ggacgcggtg gtgtacttcg acccgaaagg ggacgcgtgg
     1741 gcaccgcacg tcttccgggc gcactgcccg gacttcaccc tgctggatct gcggccgggg
     1801 aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgccctgaa gaatctgctg
     1861 gttgccgggt ttaacctgag tgagaccggc gacgtggcgg accactaccg catcagcgag
     1921 cagaaagcgg cgaagctgat tgcggagcag ttcccgcagg gggcaaacat tcagcaggtg
     1981 ctggcggcgg catacgcgct gccggaggcc ctgaaaaagg acgtgaaggg gctgatcacc
     2041 aagctggaga acgtggccga cctgagcgtc ctgcagacgg acagcggcat cgacgtggcg
     2101 gggataatca acggcggcgg ctgcctgtac gtcatcggct cgatggacga tgaggcggtg
     2161 atccgggtgc agaagatgtt gtttgcccgc tgcgcgcaaa tcatcattgc gcgggatgag
     2221 ttccggaggt ggccgcacgc gagcatcatg ctcgatgaga ttaaatatct gctgtcgaaa
     2281 tacgtgctga acgcgctggg gacgctgcgc agccgggact gcaacctgct gctcgcgcac
     2341 cagtcgctag gcgacttcgg ccagtgcggc caggatctgc ccgctgattt tgtgaaaacc
     2401 acggtactgg ataacacgcc gatccgctgg ttctatcgcg cggccagcca ggagtccgcg
     2461 cagtgggcgg cgggccagac cggggagatc cgggtggacg tggagcgccg ccgggccagc
     2521 cgggaggcgg ggaacgtgga gcacatcagc ggcgacagct tcatccagaa ggaggcgcgg
     2581 ccgctgtttg acgtgaacac cctgcagcac ctgcctgacg ggttcgcggt gatgaccggg
     2641 ctgggcgtgg cgcggctggc gttcagcagc ccgctgcggg ttgatcgccg ggaaattccg
     2701 ctgaaaacct tcccggtgct ggcgaaaacg gacccgctgg cggagtacca gtcggaggcc
     2761 gggcggcagc ggccgggtga tgatgatttt gcggggctgt actgatggca ctggagcgat
     2821 acaacgtcag ccacgcgaag cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc
     2881 gctggctgcg ggaagagctg tgttccacgg cggagctggt tgcgcgacgg ctggggattg
     2941 cggcggtaca gccggtttac cgcttcctgg acagcctggt ggcgaaaggg ctgctggtcc
     3001 gggcgaaata cccggttgac ggccgtcaga tcagcgtgtg ggggctgacg ccgcacgggg
     3061 tggcgttctc gttcgacgag gacgagccgc tgacggacat tattccgttt cagccgtcgc
     3121 gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc ctcgccatgg
     3181 aggcccgggg ggccaccggc tggcggtacc tgcaccgtat tgccctgaaa gggatgaagg
     3241 tgccggacgc gctggcggag ctggacggca gaacggtggc gtttgaggtg gagcgcaccg
     3301 tgaagagccg caggcgctat caggaggtgg tgtccgggta tctgtttaac cgccgggcga
     3361 acggcatcga tgaaatctgg tacatctgcc cggaccgggc gacgcaggtc agggtgcagc
     3421 gggcgatcct gtcggtggat gaaatcgtga atccacagac cggcgaggcc cgcaaaacgg
     3481 cggagctgga tcgtgaacgg ctgtttgcgt gctttaaatt tatgaccaca gagtgaggga
     3541 ttatggcgct gtacatggta tttattgaag atgcggagga ccgggacggc gagcgctgca
     3601 tcatgccgac cagcgagcat cttgaagata tggtggtgct cgcctggcag accggggcgt
     3661 atgttttctg ttttgtcgac cggacccagc atctggcgat gatgaaatcc atcgggatgt
     3721 atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcaccggt gaaaatccgc
     3781 aatgggaacg ggacaaaccc gcaggaatga agctgtgtga ttacctctat cgcaaggtgg
     3841 agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc
     3901 agattagcgc gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg gtgaacgaga
     3961 agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg ccggtgcctg
     4021 aactgccgga ggacaaatcc gcccgccggt ggtggccgtt cggcaaaaaa taaacgccag
     4081 caccggccat gccggtgctg ttattcctcc ccgagcttat cccgcatcac ctgccagagt
     4141 gtttccgcat cctcgtgcag ccgctcgcac atgtcggccg gggtatccat atccagctcg
     4201 ttcagtttct cgagcaacag cagcgactgg tccttaatga atttagccat attgagggca
     4261 gttttttcct gttttgtcat gattctttcc ttccgggcat ggtgtgcgcc tgtcgggtat
     4321 actgtctcaa aaatagcgga gtgagacaat ggcgtgggtg acggtgagac aggcgacgga
     4381 actgacgggg aaagcccgga gttccctgta tcgggatatg gcgaaaggcc gcgtatcata
     4441 ccgtacggaa gctgatggtg gtcgggtggt ggataccagc gagcttatcc gggtgtacgg
     4501 tgagttaaga cagggggaga ctcacgagcg ggacgggttg agactgccgg atgagactga
     4561 aaaaacggcc tccgacgcgt tgatcgcgga gataaaagca ctcagggagg aggtggccgg
     4621 actgcggcag gaaatgcagg ccatgagact gctggaacac aaaccggaga cagcagagac
     4681 accggccgga acaccgcgtc ggtggtggca gtggggaaaa cgctgacgcg ctgtgcttgt
     4741 ccaaccggcg acggccacgc aatgaattgc gcccccgtca ccggttatca gcgaacggta
     4801 tggggattct ggcgctggcc aaccacggcc agaacccgga ctttcctggt ctggtcatca
     4861 acatcataca ggacatgctg tcccatcaga ctgatccggc gaacgtcctc gccatagcgt
     4921 gagtccactt catagaggcg cgggaagtcg gccagtttgt ccgccgcagt gagaacgcga
     4981 tcggtaacgt tctgcgcatc ccacagggat gaattttccg cgatatagtc aaacagcatc
     5041 gttacatcct cgatggctcc ctgcttccat tccacatcat acggcatcgc gcagttcctg
     5101 ataacggcca tgcaggcgct ggcggagttg ttccgtggtc atgcccggac gcgtgtcagc
     5161 acgggcggcg gttactttgg cgcgaacctg atcttcatac tctgcctggg ccagttcttc
     5221 gttagccagg cgttgcaaca acgcagcttt gtccgggtca ctttccggca gtatgcgggg
     5281 caggttttca gccagttccc gcaggaagtt ggcgttttcg tagtgtgtag tgttgctcat
     5341 tgcggaggct cccggattaa tgcggtgata tgaagaagtt tagcaggggg cggtctgaaa
     5401 gaatatggtc gtgtcagtca ggctgatgaa gcagaaagtc ggtaaaaaaa tggcttacgg
     5461 catccatttt acgtcaaaac atatgctatg cagttgcgtg cataggcatg cattaggata
     5521 aagtttaccg ggcgcgtttc cggcggtttt ccgggtgatt tgttgcctgt tttatcccgt
     5581 aggcgccgga aacgccctca gcgcgtttct gacgatgctg ataacgggtt ttatggtaaa
     5641 tggctggtta tgaggcattc agctgacgat ttttgcgtca acttttcgct gtatgtttaa
     5701 acatgagttt aacattagcg tcatctgata gcgctgttgc taatttcatc gacccgacag
     5761 gtaatttcct tgtaaatcaa gcgcgaaaga tcagcaaaaa agctatgtgt gcgcttatgg
     5821 aataaggtag aataagggat tctgatgatc gtgaggttgt cagtgaaaaa cgaggcagct
     5881 atcagatctt cggcctgaga aactaaggat tctgatagct gccggtgact cagttcgtga
     5941 aaactgaatc ttacctgatc aacaaacgcc cgattagggc gtttgtcaag tggtgctgca
     6001 ttatgtagta acttggtacc gacttgccag gcatttttca cagttttttg atgtgcgaat
     6061 gctcatccgg acaccgtcac ctgttattta tcctctgtaa acaggtgcta taaatgcaaa
     6121 aagtaactga tctgtatcct cctgaaattg ctcgccataa gctccagaat cacttcgccg
     6181 gtaataccgt tatgctggag ttaatccaga aactaaataa aaccagcctg tgtacttttg
     6241 cggcgctctg tgacggcaac gtagtgacca cttccggcta taacatcacg gccgatctat
     6301 gtgttaaccg tgcgtcagcc gtggcccatt cgcttaagca aaaatatctg ccagttacgg
     6361 ccagaacgat ttcgacaaag gctgatgtag gtggtgcggt aaagcaggcg gctttctgta
     6421 ttgatgagga cgatcttgca aggctgaaat cagagccgga aaagatgatg aaagagtgcg
     6481 aacgtaacct gaacagccag aagcgcacca atgctcagaa ggaaatgagc cggttgtaca
     6541 aggagtttgg tgaagacggc atactggcgc tgttacgcaa tgttgcccag tcgaacggta
     6601 caccgccgcc aggcgcacac cctgcaagtt gataccagta aagcccctaa ggggctttat
     6661 ttttaaggtt ttctggatgc cgccagctct tgatctgtca gctaatcgcg gtgcagatag
     6721 ttcccttcta ccggcggtgg cgttaccagt tttgcttttc cttctttcgg acgagaattt
     6781 cactcttacc gtacattttc gtacggttgg acctcgaacc cctttcaggc ccatggccag
     6841 tgcctgagcg acaaaggcgt ccgccaggcg cttttcttca ggtgcgtcat ggtggcctcc
     6901 gggttgtctc cgattcctga acagtttaac cgcaggtact ctcctcatgc catttcgctt
     6961 tcaggtacat ctataccaat ataaaagtaa gtgtgatgaa tgtctcaaat aataaaattt
     7021 gttgccatga gaaattttcg ggaaaagacg cgatctgctt acaggcggct ccgggcgctt
     7081 ttttgtgcct gaagatgaga actaatctca tatattgaca cctgaaaact ggaggagtaa
     7141 ggtaataatt atactgtgca tatatacagt atattggttt taatttattg ttttaaatgt
     7201 caaagaggaa aacgatatga gtggtggaga cggtcgaggt ccgggtaatt caggtctggg
     7261 acataatggt ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag gtggcccttc
     7321 atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacgccgaa
     7381 tggagatatt cataactata atccggggga gtttggtaac ggcgggagta aacccggtgg
     7441 taatggtggt aacagcggca atcatagcgg tagctctggt ggcggacagt cttcggccac
     7501 tgcgatggcc ttcggtctgc ctgccctggc tactccgggg gctgaaggac tggctttatc
     7561 agtttccggc gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc tgaaggggcc
     7621 gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg agattgcaaa
     7681 agatgatccg aaaatgatgt caaaaattat gacgtcatta ccggccgatg cggtgacgga
     7741 gactccggca agtactttac cactggacca ggcgacggtt cgtgtcagac aacgggttgt
     7801 ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc caatgagtgt
     7861 ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg
     7921 tctcccgtct ctgcaggtga acgtaccgaa aggtgttccg acagcgaaag ccccgccaaa
     7981 aggcattgtt gctgaaaaag gtgattcacg tccggctggt tttacagccg gtggtaactc
     8041 ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag aagccggttt atgtgtcggt
     8101 gacagatgtt cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa agcgtcgcca
     8161 gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg ataaagcgaa
     8221 agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca ttgcatcgac
     8281 agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga
     8341 ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg
     8401 gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa
     8461 agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata aggctgcact
     8521 taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa
     8581 gttaaatgag gaaaagaaca agcctcgcaa gggagttaaa gattacggtc atgattatca
     8641 tcccgctccg aagaccgaag aaatcaaggg gttgggggaa ttaaaaaaag cacctaaaaa
     8701 aacacctaaa caaggtggtg gtggtagacg tgaccgttgg attggtgata aaggccgtaa
     8761 gatttatgaa tgggactccc agcacggtga gcttgaaggg tatcgtgcca gtgatggcga
     8821 acacctcggg gcatttgatc caaaaacggg taagcaaatt aaaggtccgg atccgaaagg
     8881 acgaaacatt aaaaaatatc tttgagaggt aagtatgggg cttaaattaa atttaacctg
     8941 gtttgataag aagactgagg aatttattgg tgaggagtat tctaaagact ttggtgatga
     9001 tggttctgtc attgaaagcc ttggaatgcc tttaaaggat aatattaaca atggcgggtt
     9061 tgatgttgag aaatcatggg ttccgttgtt gcagccatat tttaaaaata aaattgaagt
     9121 cgataagtac tggtatcaaa tttcatttga ttatcgggac ggtaactggt aacaaaaaat
     9181 tatcaaggat gatttgaaat acagacttcc ggtgttcagg gatgaatgcc ggggttttta
     9241 aatacaggga aggtatatga aaaaggtaaa ggttgttttt ctttttatat tgattttttt
     9301 aggctttctt ctggtcgcat gtcaggcaaa ctatatccgg gatgttcagg gtggaacggt
     9361 ggcaccatcg tcctcctctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga
     9421 tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc
     9481 tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacc gtggtaactg
     9541 gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccggact ttggcgcaca
     9601 cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg cgaactccca gttaccaatg
     9661 gctgctgcca gtggcgtttt gcgtgctttt ccgggttgga ctcaagtgaa cagttaccgg
     9721 ataaggcgca gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa
     9781 cgacctacac cgagccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgc
     9841 aagggagaaa ggcggacagg tatccggtaa acggcagggt cggaacagga gagcgcaaga
     9901 gggagcgacc taccggaaac ggtggggatc tttaagtcct gtcgggtttc gcccgtactg
     9961 tcagattcat ggttgagcct cacggctccc acagatgcac cgaaaaagcg tctgtttatg
    10021 tgaactctgg caggagggcg gagcc
//
