LOCUS       CP052397                2789 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C17KP0040 plasmid pC17KP0040-4,
            complete sequence.
ACCESSION   CP052397
VERSION     CP052397.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609902
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:54:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,596
            CDSs (total)                      :: 5,473
            Genes (coding)                    :: 5,343
            CDSs (with protein)               :: 5,343
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 62 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 23 of 130
            Pseudo Genes (multiple problems)  :: 14 of 130
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C17KP0040"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC17KP0040-4"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            257..454
                     /locus_tag="HJX14_27945"
     CDS             257..454
                     /locus_tag="HJX14_27945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK31469.1"
                     /translation="MERVLGIYVVGIIVSAGFLFIQEVRNGRRRSYTEEGASMLVGIIC
                     AFLWPLVLIAYFFDKKNGKL"
                     /name="hypothetical protein"
     gene            451..870
                     /locus_tag="HJX14_27950"
     CDS             451..870
                     /locus_tag="HJX14_27950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK31470.1"
                     /translation="MKLSLFFISVITFLLAISPIAYSDNTIIPDRVYDPYRNSAVTPDG
                     TVYDRLTGGVLKASEHPQPNFGFPVQSSSDSCDTTYVKARTEDGRLLVTSDGVTLHVVD
                     NIVDVAHWQPNDELRVCGDRVINIDDNTSSSFTYN"
                     /name="hypothetical protein"
     gene            complement(1161..1292)
                     /locus_tag="HJX14_27955"
     CDS             complement(1161..1292)
                     /locus_tag="HJX14_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJK31471.1"
                     /translation="MHEPPVQSDYHALFGNYRLESNPKRHDKSPLAVAIGRGCTRDY"
                     /name="replication initiation protein"
     gene            complement(1317..1740)
                     /locus_tag="HJX14_27960"
                     /pseudo=""
     CDS             complement(1317..1740)
                     /locus_tag="HJX14_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            1697..1888
                     /locus_tag="HJX14_27965"
     CDS             1697..1888
                     /locus_tag="HJX14_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK31472.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFCTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1888..2289
                     /locus_tag="HJX14_27970"
     CDS             1888..2289
                     /locus_tag="HJX14_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJK31473.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(965..1474)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.038"
                     /coverage="99.0"
                     /evalue="1.71e-152"
ORIGIN
        1 tgcgtccccg ttcaccgttt tcggatggaa agaacgccgg aaacaggact tactctggct
       61 gaatggcgtg aattttcgcg ctatgcactt gcgtgcatac tcatgcatgc cttaaaaaca
      121 gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctgttttac cggtttcccc
      181 tcagaaacgc cctgagaccg ttttagcggt gcgtacaagg gatgttatgt ttaatctgat
      241 aagaacggaa tgttttatgg aaagggtatt aggaatttat gttgtcggta ttattgtgag
      301 tgccggtttc ctgtttatcc aagaagttag gaacgggagg cgcagaagtt acaccgaaga
      361 aggtgcgtca atgcttgtcg gcataatttg tgcgtttttg tggcctttag ttttaattgc
      421 atattttttt gataaaaaaa atgggaaatt atgaagttaa gtctgttttt catctcggtc
      481 attacttttt tattggctat atcaccgata gcatactcag ataacacgat tattcctgac
      541 agagtatatg atccttatag aaattcggct gtaactcctg atggcaccgt ttatgaccgt
      601 ctgacaggag gagttttgaa ggcatcagag caccctcaac ctaattttgg gtttcctgtt
      661 caatcctcat cggatagttg tgatacaacg tatgttaaag ctaggacaga agacggtagg
      721 cttttagtca cgagcgacgg agtaactctg catgtagttg acaatattgt tgatgttgct
      781 cactggcaac caaatgatga gcttagagtc tgtggcgata gagtaatcaa tattgatgac
      841 aatacttcct cctcgttcac atacaattaa cctcttttat tcaaagaata tctatggtga
      901 tgatagaaag attgacgagc atgtatgggg ggatagccga agaaatgaac caaagttcaa
      961 aaggatcttc ttgagatcct ttcaggagcg tcgtaatctc ttgctctgaa aacgaaaaaa
     1021 ccacctgggg aggtggtttt tcgaaggttc agtaagttgg ggaacttctg aaccgtggta
     1081 acagggtgta caagaccgct gccaccaaat tcgtcctttc agtttagccg cagtctggct
     1141 ctaacttcaa gaactttgca tcagtaatct cttgtacacc ctctaccaat ggctaccgcc
     1201 aatggcgatt tgtcgtgcct tttcgggttg gactcaagac gatagttacc gaaaagagcg
     1261 tggtagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     1321 ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc
     1381 ggtaaaccga atggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg
     1441 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg
     1501 cttgtcaggg gggcggagcc tatggaaaaa cggccgctgt gctgccttct ccctctccga
     1561 gctgtctgct ctatgtcgtt atgtgcgcgt tgcgaccgct cgccgcagcc gaacggcagg
     1621 agcgtagcga ctgagtgagc gaggaagcgg aaaagagatc tgtctgacat tgagcactga
     1681 cgctctgagg ccctttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat
     1741 ccagaatcag tctctgctcc tgctggagaa gcttaacgag cttgatctcg atgtcgaggc
     1801 cgacctttgt gagaaactcc acgatgatgc ggagcatctt ttctgtacac tgaccgcgag
     1861 actggatgca ttacaggatg gtaactgatg gccagagtgt ctattagtga ggctgcccga
     1921 ctgacgggta agagtagaac aacactgcac aggttaatta aagcaggtga cctgtccacc
     1981 tgtagcggtg aacgtaatgc aaaaatggta gatacttcag agcttttacg tgtctttggc
     2041 ccctttgaac aactgaaggg tgaacaccat tctggacagg tctctgaaca gcgtgataca
     2101 gcctctgctg accagagtga acaggtgatt cgtcatctga aacaggagat tgaacacctg
     2161 aacaccttac ttttgtccaa ggattcgcac attgacagcc tgaaacaggc catgctcctt
     2221 cttgaaaatc accaggcaaa attaactgaa tccccggcgc catggtggaa attctggaaa
     2281 aaatcctgac cccgtcatgc ttcagttttc ctctccggcc atcccaggga gttcctgaac
     2341 cttcccaaaa atcgtcagat ctctgaacgc tcctcaccac taaaaatacc cacaccccac
     2401 gcaaaattaa aattttgcag attccttatt tacaacaatg ggttaatgaa aaataaatgt
     2461 gcggtgatgt gttacaaagt gcagatccaa gtcgtggacg actctatggc gaatcgctga
     2521 cgaattgccc acgattcata tgtgaatcac gctttattat catattgtta tgatgaatcg
     2581 actcttgatt caattcatca ctttgggtgt tttttgctca tggtgactct tgattcaatt
     2641 cacgattcga atcgccggtg aatcgtgtgg tgagtcgtcc ggcgacttgc cataacgtga
     2701 ttgccggccg tcgacgaacc cgattcctga acgtgctgaa aaccggtttt ccggctgcgc
     2761 cggcaacgct tcacggccac gcaataaat
//
