LOCUS       CP052396                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain C17KP0040 plasmid pC17KP0040-3,
            complete sequence.
ACCESSION   CP052396
VERSION     CP052396.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609902
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:54:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,596
            CDSs (total)                      :: 5,473
            Genes (coding)                    :: 5,343
            CDSs (with protein)               :: 5,343
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 62 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 23 of 130
            Pseudo Genes (multiple problems)  :: 14 of 130
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C17KP0040"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC17KP0040-3"
                     /geo_loc_name="South Korea: Gangwon"
                     /lat_lon="37.82 N 128.16 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(9141..9294,1..146)
                     /locus_tag="HJX14_27890"
     CDS             join(9141..9294,1..146)
                     /locus_tag="HJX14_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJK31458.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(208..585)
                     /locus_tag="HJX14_27895"
     CDS             complement(208..585)
                     /locus_tag="HJX14_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJK31459.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            654..833
                     /locus_tag="HJX14_27900"
     CDS             654..833
                     /locus_tag="HJX14_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK31460.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(861..1391)
                     /locus_tag="HJX14_27905"
     CDS             complement(861..1391)
                     /locus_tag="HJX14_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK31461.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1398..2129)
                     /locus_tag="HJX14_27910"
     CDS             complement(1398..2129)
                     /locus_tag="HJX14_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJK31462.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(2129..4093)
                     /locus_tag="HJX14_27915"
     CDS             complement(2129..4093)
                     /locus_tag="HJX14_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJK31463.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5574..5723)
                     /locus_tag="HJX14_27920"
     CDS             complement(5574..5723)
                     /locus_tag="HJX14_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJK31464.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5808..6065)
                     /locus_tag="HJX14_27925"
     CDS             complement(5808..6065)
                     /locus_tag="HJX14_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK31465.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(6075..7760)
                     /locus_tag="HJX14_27930"
     CDS             complement(6075..7760)
                     /locus_tag="HJX14_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK31466.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            8163..8582
                     /gene="exc2"
                     /locus_tag="HJX14_27935"
     CDS             8163..8582
                     /gene="exc2"
                     /locus_tag="HJX14_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJK31467.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            8888..9151
                     /locus_tag="HJX14_27940"
     CDS             8888..9151
                     /locus_tag="HJX14_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJK31468.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     BGC             1075..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4893..5471
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1402..2129)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atgaagtgga ctcacgctat ggcgaggacg ttcgccggat cagtctgatg ggacagcatg
       61 tcctctatga tgttgatgac cagaccagaa aagtccgggt tctggccgtg gtcggccagc
      121 gccagaatcc ccatattgtt ctctaataac cggtgacggg gacgcaattc attgcgtggc
      181 cgtcgccggt tggacaagca cagcgcgtca gcgttttccc cacggccacc accggcgcgg
      241 tgttccggcc ggggtctctg ctgtctccgg tttgtgttcc agcagtctca tggactgcat
      301 ttcctgccgt agcccggcca cctcctccct gagtgctttt atctccgcga tcaacgcgtc
      361 ggaggccatt ttttcagtct cacccgacag tctcaactcg tcccgctcgt gagtctcatc
      421 ctgtcttaac tcaccgtaca cccggataag ctcgctggta tccaccaccc ggccgccatc
      481 agcttccgta cggtatgata cgcggccttt cgccatatcc cgatacaggg aacttcgggc
      541 tttacccgtc agttccgtcg cctgtctcac cgtcacccac gccattgtct caccccgcta
      601 tttttgagac agtatacctg acaggcgcac accatgcccg gaaggaaaga atcatgacaa
      661 aacaggaaaa aacagccctc aatatggcta aatttattaa ggagcagtcg ctgctattgc
      721 tcgataaact gaatgagctg gatatggata ccccggccga catgtgcgag cggctacacg
      781 aggatgcgga aacactctgg caggtgatgc gggataagct cggggaggaa taacagcacc
      841 ggcatggccg gtgctggcat ttattttttg ccgaacggcc accaccggcg ggcaggtttg
      901 tcctccggca gttcaggtac cggcacgtta aaatagcggc tgttatattc ccgcatcagc
      961 tccgctttca gcttctcgtt caccgcgctt tccccgtgcc gctcactcac cacgtcaatc
     1021 atcgcgttaa tctgctccgg tgagcacttc ccggcatcaa ccacctgcac accgttctcg
     1081 atggcgaacc gctccacctt gcgatagagg taatcacaca gcttcattcc tgtgggtttg
     1141 ttccgttccc attgcggatt tttaccagtg aaaaagtacg tggctttttc gtcgctgttg
     1201 agccgctcgt catacatccc gatggatttc atcatcgcca gatgctgggc ccggtcgaca
     1261 aaacagaaaa catacgcccc ggtctgccag gcgagcacca ccatatcttc cagatgctcg
     1321 ctggtcggca tgatgcagcg ctcgccgttc cggtcctccg cgtcttcaat aaataccatg
     1381 tacagcgcca taatccctca ctccgttgtc ataaatttaa agcacgcaaa caaccgttca
     1441 cgatccagct ctgccgtttt gcgggcctcg ccggtctgtg ggttcacgat ttcatccacc
     1501 gacaggattg cccgctgcac cctgacctgc gtcgcccggt ccgggcagat gtaccagatt
     1561 tcatcgatgc cgttagcttt gcggttgaac agatacccgg acaccacttc ctgataccgg
     1621 cgacggctct tgacggtgcg ctccacctca aacgccaccg ttttaccgtc cagctccgcc
     1681 agggcgtccg gcactttcat gcctttaaga ctcagacggt gcagaatgcg ccagccggtg
     1741 gcccctctgg cttccatatc aaggcgcagg gattgcacgg ccagccggtg cggcagatgt
     1801 gctgccgaca cccttgacgg ctgaaacgga ataacatccg ccagcggttc gtcctcgtcg
     1861 aacgagaacg ccacgccatg cggcgtcagc ccccacacgc tgacctgacg gccgtccacc
     1921 ggatatttgg tccggaccag cagccctttc gccaccaggc tgtcgaggaa gcggtagacc
     1981 ggctgcaccg ccgcaatccc cagccgccgc gcaaccagct ccgccgtgga acacagctct
     2041 tcccgcaacc agcgcagggt cagccgggtt ttctccgcgt tccgcgcctg tcgtttcgcg
     2101 tggctgacgt tgtacagctc cagcgccatc agtaaatccc cgcaaaatca tcatccgctt
     2161 tcggcccgcc gccgtcggcc gggggccgga actccgccag gggatcggtt ttcgccagca
     2221 ccgggaaacg ctgcaacggg atttcccggc gctcaacccg cagcggactg ctgaacgcca
     2281 tccgggccac acccagcccg gtcatgaccg cgaacccgtc cggcaggtgt tgcagggtgt
     2341 tcacgtcaaa cagcggccgc gcctccttct ggatgaagct gtccccgctg atatgctcca
     2401 cgttccccgc ctcccggctg gcacggcggc gctccacgtc caccctgatt tccccggtct
     2461 gccccgccgc ccactgcgcg gactccaggc tggccgcccg gtaaaaccag cggatcggcg
     2521 tgttatccag taccgtggtt ttcacaaatt cagcgggcag atcctgcccg cactgcccga
     2581 aatcgcccag cgactggtgc gcgagcaaca gattgcagtc ccggctgcgc agcgtcccca
     2641 gtgcgttcag tacatacttc gacagcagat atttaatttc atcgagcatc acgctgacgt
     2701 gtggccactc ccggaactcg tcccgcgcaa tgatgatttg cgcgcagcgg gcaaacagca
     2761 ttttttgcac acggatcacc gcctcgtcgt ccatcgagcc gataacgtac aggcagccgc
     2821 cgccgttgat aatccccgcc acatcgatgc cactgtccgt ctgcaggacg ctcaggtcag
     2881 cgacgttttc cagcctagtg atcaacccct tcacgtcctt tttcagggcc tccggcagcg
     2941 cgtacgccgc cgccagcacc tgctgaatgt ttgccccctg cggaaactgc tccgcaatca
     3001 gcttcgccgc tttctgctcg ctgatgcggt agtggtccgc cacgtcgccg gtctcgccca
     3061 ggttaagccc ggcaatcagc aggtttttca gcgcgtactg gtcgagatcg cggaacagat
     3121 tcagctgcgc cggtttcccc ggccgcagat ccagcagggt gaagtccgga cagtgcgccc
     3181 ggaagacgtg cggcgcccat gcgtcccctt tggggtcgaa gtacaccacc gcgtccccga
     3241 aatcgcgtac gcagcgaatg agcgcgttgg tggccatcac gctcttgccg ctcccgggca
     3301 ggccgaggat ctgaatgttg gtcttgcgcc aggtatcacg cggaaccagc accggcttgc
     3361 cctgctcatc cttacccaga aacaccgcct gctcctgccg ggcctgctcc gaggaaaaat
     3421 cggccagcgc attaaccggt accggctccc cgaaatgcag gtggcgcacg tcggtcatct
     3481 ctcctgtccg ctggctgctg cgccggctct cctgattcag tttcgcgttc accgcctcgt
     3541 cagcagccgc aaaatgaccg tcaagcagca ggaacgccag cagccgcgcc aacacaatcc
     3601 cgacagccag cccgcccgcg caccaggcaa tcccgaacag gttagccggc tgcagcaggt
     3661 aataatctgc gtttgcgtag aggtccggac gcaggacggt ttctccccag ctctcgataa
     3721 acacggcgac aaccagcccc cccagaaggg ccagaccggc cgcgccggtc agccagtacg
     3781 cgtagttcca gacatgtcga cgtgccctga ccatccgccc gggcgaatcc agcgaggcca
     3841 gcaccgaaaa tggcgctggc cgtttattat cgcgcttata gcgatcggca tactcccgtc
     3901 gctccttatg actcagcccc acaaaacgca tccgtccggc ccaaagttca gccagaccga
     3961 aaaacaggcc gataactagg aaagccccgg ccagaccgta atactcctgg tacagccagc
     4021 tgacccacgg ataaatctgc cgggccagct ccggaatgtg ccacttccag gcaagcgaat
     4081 ccgtattaaa catgttctct ctccttcaga cgatggtttg ccggcgtgat gtgcagcccc
     4141 cgctgcacaa gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat
     4201 tgcgttcagc tttcaccacg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc
     4261 cccggaggaa caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg
     4321 tcttttccgg tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca
     4381 aaatcaggag cagctttgct gcttcgccag gtgcgtccag caccgtggat ttttgtgggt
     4441 ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg
     4501 acggagccag aaagaggggg agagaccgtg cgtcggtggc gtccagccag cgtaagcagg
     4561 ggatatcccc ctgaggtcag ccacgccctc gcggggatga cctgtatttc tccgtaagga
     4621 gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga tatgattcac
     4681 ctatcctggt aatttattat cagggaatat aaagacttaa tcaggggaca atatgccaca
     4741 tcagcgcgtc cggtgtccgt cttgtaggtg ccggggaaat tgcgcctcct cgctcactcg
     4801 gtcgctacgc tccggccgtg agactgcggc gggcgctgcg gacacatgca aagttaccca
     4861 cagattccgt gaataagcag gggactaaca tgtgaggcaa aacggcaagg ccacgcaggt
     4921 ggcgtttttc cataggctcc gccctcctga ctgagttcac ataaacagac gctttttcgg
     4981 tgcatctgtg ggagccggtg gctcaaccat gaatcagtca ggagtggcga aacccgacag
     5041 gacttaaaga tcatcaccgt ttccgatgaa tcgctccctc ttgcgctctc ctgttccaac
     5101 cctgccgttt cccggatacc tgttccgcct ttctcccttg cgggaagtgt ggcgctttct
     5161 catagctcac actctggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta
     5221 agcaagaacc ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
     5281 ccaacccgga aaagcacgca aaacgccact ggcagcagcc attggtaact gggagttcgc
     5341 agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac
     5401 actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt
     5461 tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg
     5521 gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga
     5581 accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccacc ctgaacatcc
     5641 cggatatagt ttgcctgaca tgcgaccaga agaaagcctg aaaaaatcaa tataaaaaga
     5701 aaaacaacct ttaccttttt catatacctt ccctgtattt aaaaaccccg gtattcatcc
     5761 ctgaacaccg gaagtctgta tttcaaatca tccttgataa cttttgctta ccagttacca
     5821 tcccggtaaa caaaggatac aaagtaatca aatttactaa tatcgattac atttttaaag
     5881 tgtggctgta atatcgaaac ccatgatttt tcaacatcaa accaaccatt attaatatta
     5941 tcctttaaag gcatccccag actttcaatg acagaaccat catcaccgaa gtcttttgag
     6001 tattcaccac ctttaaactc ttcggttttc ttatcaaacc aatgaatatg taatttaagc
     6061 cccatattta cctcttaaag atatttttta atgtttcgtt ttggatccgg ccctttaacc
     6121 tgcttacccg tttttggatc gaatgccccg aggtgttcgc catcactggc gcggtaccct
     6181 tcaagctcac cgtgctggga atcccattca taaattttac gctttttatc accataccat
     6241 cgggcccgct taccaccacc accttgttta ggggttttag gtttaccctc tttcaactct
     6301 cccaacccct taatatcttc agtcttggga tcaggaaaat aatcatggcc gtaatcttta
     6361 gttcccttac gaggtttttt cttttcctca tttaatttat tttctgcatc cttcgctttt
     6421 ttctcctttt gtttccggct ctccatcgcc ccggaaagcg cagccttatc cctggataag
     6481 gattcattca tttcgtttaa agatgtctgg gcagctttta atttctcatt ctgattctga
     6541 atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg
     6601 ttataagtca caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc
     6661 tgaacagcag cccttgcacc ggggatcgcc ttctctgtcg atgcaatgcg actgtttaag
     6721 gtcgcaatat ttttatcttc agcatccagc tcagctttcg ctttatcata ctctcttttc
     6781 agcccctctt ccgggtgagc agcgtcccat gcctgctggc gacgcttttc ttcctccaga
     6841 cgctgtttta cctgtgccgg agtaagaaca tccgtcaccg acacatagac cggtttctgt
     6901 ccgctctctt tcgggaaacg aataacggcc tcacgggagt taccaccggc cgtaaaacca
     6961 gccggacgtg aatcaccttt ttcagcaata atgccttttg gcggggcttt cgcggccgga
     7021 acacctttag gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc
     7081 cccggacgtt ttgtcggttt cgcatccacc acaggaacac tcattggccg gccggtgaca
     7141 accgcaatat gctgccgctc gtccttcaca atatccgcca cacgtttagt gacgctgacc
     7201 gtcgcctggt ccagcggcag ggagctgacc ggcgtctccg ttaccgtgtc tgccggcaat
     7261 gacgtcacga tttttgacat cattttcgga tcatcttttg ctatctcaga aggcagcact
     7321 ccgtaaatcg caatccccca cagaccaaac ttaaacggac ctttcagggc agccagcaca
     7381 tcagcaacag cggccgacaa cgcatcgccg gaaacggata aagccagtcc ttcagccccc
     7441 ggagtggcca gggcaggcag accgaaggcc atcgcggtgg ccgaagactg tccgccacca
     7501 gagctcccgc tatgattgcc gctgttaccg ccattaccac cgggtttact cccgccgtta
     7561 ccaaactccc ccgggttata gttatgaata tccccgttag gtgtatgcgt cgtaccccag
     7621 cccggcccgc tgtttggatc cgtaccacca cctgatgaag ggccaccttt acctgatgta
     7681 ccgttcacat tcccactggc ctgaccacca ttgtgtccca gacctgaatt acccggacct
     7741 cgtccgtctc cgccactcat atacttttcc tctttgactt ttaaaacaat aaactaaaat
     7801 aaaatactgt acatataacc actggtttta tatacagtat aaaaattacg ctgccttgtt
     7861 tttcatgtca agtctgtaaa tttgcgtttt tgtgaaaatg atcaaaaaaa cagccactca
     7921 caccatgcgc aatcccctcc agatgcaaca aaaaccagca caaacggcgc cgttctccgg
     7981 cctcagtatg ttttactgaa atcattatat gagataccag aaagccccct ccgggacagg
     8041 aaaagaaacg gggggtctgg ttcgggttgt tcggtcactg aacacggtca gaaactgatg
     8101 ataagatagc ctgcggatat ataactcaat gagaaaaaag aattaaaggt aaggagacgc
     8161 tcatgagacg ttccggcagc aaactgtgtc ttatcgccat gataacagcg acagcaactc
     8221 tcactgcctg tgctccaaag ggcagcgtgg aacagcatac ccggcattac gtatatgctt
     8281 cagatgacgg ttttgatccc aatttttcca cccagaaagc tgacacaaca cgaatgatgg
     8341 tgcctttttt ccggcagttc tgggatatgg gcgcaaagga taaagcgacc ggaaaatcac
     8401 ggagtgatgt gcagcaacgc attcagcagt ttcacagtca ggaattttta aactcactcc
     8461 aaggcacaac acaatttgcg ggtaccgatt accgcagcaa agacctcaca ccgaaaaaat
     8521 caaggctact ggctgacacc atttcagctg tttatctcga tggctatgaa ggcagacagt
     8581 aagtggattt accataacgc gtcattacgc gcaccgctca gacgggctca gggcgtttcc
     8641 ggcggctacg ggataaaaca ggcaacaacc cccccggaaa actgccggaa acgcgcccga
     8701 taaattttat cctaatgcat gcctatgcac gcaactgcat ggcatatgtt ttgacgtaaa
     8761 atggatgccg taagcccttt tttaccgact ttctgcttca tcagcctgac tgacacgacc
     8821 atattctttc agaccgcccc ctgctaaact tccttcatat tgccgcatta acccgggagg
     8881 ctccgcaatg agcaacacta cacactacga aaacgccaac tttcttcggg aactggctga
     8941 aagcctgccc cgcatactgc cggaaggtgg cccggacaaa gctgcgctgt tgcagcgact
     9001 ggctaacgaa gaactggccc aggcagagta tgaagatcag gttcgcgcca aagtgaccgc
     9061 cgcccgtgct gacacgcgtc cgggtatgac cacggaacaa ctccgccagc gcctgcatgg
     9121 ccgttatcag gaactgcgcg atgccgtatg acgtggaatg gaagcaggga gccatcgagg
     9181 atgtaacgac gctgtttgac tatatcgcgg aaaattcatc cctgtgggat gcgcagaacg
     9241 ttaccgaccg ggttctcgct gcggcggaca aactggccga cttcccgcga ctct
//
