LOCUS       CP052385                3374 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0008 plasmid pD16KP0008-4,
            complete sequence.
ACCESSION   CP052385
VERSION     CP052385.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609906
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,311
            Genes (coding)                    :: 5,197
            CDSs (with protein)               :: 5,197
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 63 of 114
            Pseudo Genes (incomplete)         :: 51 of 114
            Pseudo Genes (internal stop)      :: 26 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0008"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0008-4"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(110..343)
                     /gene="mbeD"
                     /locus_tag="HJX18_27130"
     CDS             complement(110..343)
                     /gene="mbeD"
                     /locus_tag="HJX18_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJL10406.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="mbeD"
     gene            complement(350..910)
                     /locus_tag="HJX18_27135"
     CDS             complement(350..910)
                     /locus_tag="HJX18_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJL10407.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSQQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="mobilization protein"
     gene            complement(879..1430)
                     /locus_tag="HJX18_27140"
                     /pseudo=""
     CDS             complement(879..1430)
                     /locus_tag="HJX18_27140"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1588..1911)
                     /locus_tag="HJX18_27145"
     CDS             complement(1588..1911)
                     /locus_tag="HJX18_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJL10410.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLEEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1975..2166
                     /locus_tag="HJX18_27150"
     CDS             1975..2166
                     /locus_tag="HJX18_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJL10408.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2884..3210
                     /locus_tag="HJX18_27155"
     CDS             2884..3210
                     /locus_tag="HJX18_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJL10409.1"
                     /translation="MKLSAPVAELSRVRSPSSQLPQPQTVFANTQASAKKGFFSGVWGG
                     CEAEKFLKNTEGSCKLNELVSVAANNRLHRNIEEVENLNEPEEARLMAGFFRSTLPDER
                     SQVK"
                     /name="hypothetical protein"
     Typing          complement(2248..2328)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(28..1598)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.628"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaagatttc gtcacttccc cggtcacatc tccattgatg gcccactccg aacccgtgcc
       61 gcgcgttcct gtacctcatg tctgtaccgg gcacgctcct gttcatccct tagctgttgt
      121 tcgcgataaa taaccgactc aagcgatcca cttgcccggc taatctctgc acctgctgac
      181 tcaatatcac gacgcgctcg ttcagtatcg cgttgttccg ttgcgtaacc tcgtgcatct
      241 tctgcaattc cgcgaaggcg ctctcccact cgttcagccg ccgcatatag tcctgttgca
      301 aatgctctaa ggcgttcagc aaatgccctt ccagttccgt catactcgtt tactccctga
      361 ccagcctcac ggcgttcttg tcgcccaccg tccaggtgtt ttcccctttc accccgtccg
      421 gcaacaccag aaatttcccg ttctcgccat tgacgaacgt caccccccag gtttttgcct
      481 cgagtttcgc cagcgtgttt tcctgctctc tgatagtcag gatatttttc gcaatcttgc
      541 tttcctgcca ctccatcagc ccccagcacc ccaggaaaat cccgcccgtg acgctgagcg
      601 cggtcagcgc cggaaacagt atccgccctt tgatcacccg ccagagcagt tttttctgct
      661 gctgggacag ttcggcctct gtggtgctga actgcatgct cacggtggtg ctcagcgtat
      721 ccagttgctt tttcacggat gttgtatgtt cgctgatagc gtctctgatt ttctgcctgt
      781 ttaagctcaa tgccttgtct acagactctt cgagcttcct gaactcgctg ttcagcatgt
      841 tctcggtaga agcggctcgc tctttcagtt tcttctcgaa gtctgttccc atttgtaaaa
      901 gattgctcat acagcgcccc ctttagcctg agattccgcc ccccctccgg gtcggcaata
      961 ctgatgctgc ttttggttgt cctcaccacc tcaaaacctg ccgctgtaag cgcctctgtg
     1021 acatcctgac gcgtttttat tgctccggca tggtaaagag cctcaagacc ccgtgtaatc
     1081 gcttctgcgg cctcctgttt cgcttttggc agattatttg gcgtgacaag cgtccgtctg
     1141 ttctccggtg cgttcgggtc acgcagcccg taatgatgat taaccagcgt ctgccaggca
     1201 tttatcctcg gcctgtccgc acggtcgtag tacggctgga ggcgcttacc gctctgtagc
     1261 tccatgttcg ggatgacaaa attcaattcg agccgcccct tatcctggtg ttccacccag
     1321 agaatgctgt actggtcttt ttcgagaccg ggcataagaa cacgctcaaa gctcgtcatc
     1381 actctttcgc gttcacccgg cggtaactcc ttctccgcga acgacagaac gcctgaagtg
     1441 tatttcttcg aaaatggcgt ggaatcaatg agttcccgca cttcttccgg gttaccccgg
     1501 agcaccgttg cgccttcgcg gttacgctcc cgccccagca ggtaatcgac cgggccactt
     1561 ccaccgcctt tccccctggc atgaaattta actatcatcc cgtaccccct gttccctgac
     1621 agcctgccgc aactggcgca attcaccctc gatggccatc agtgcggcca ccacatgaac
     1681 ccggtcaatc gaagaccact ggccgctgtt caccttccgg gctgtctggt tcaggttgtt
     1741 cccgatggca gccagatgcc gcagcaatgg cggcgatagt gtcggcagtt ttcctgtgcg
     1801 tgcaaccggc tcctccagac agacccgccg catccattcc gccagtcgtt taccctcgca
     1861 gcgttccagt aaccgggcgt gctcctcatc ggtaacccgt atcgtgagca tcttctcgcg
     1921 tttcatcggt atcattcccc ctaacaaatc ccccatacaa ggagccttaa gaccatgacc
     1981 aaacaggaaa aaacagccct taacatggct cgctttatca gaagccagac gctaaccctg
     2041 ctggaaaaac tcaacgatct ggacgcggac gaacaggcag acatctgcga atcacttcac
     2101 gaccacgccg acgaactcta ccgcagttgc ctcgcacgct tcggcgatga cggtgaaagc
     2161 aactgaaaca tgcagctccc ggagacgttc acagcttgcc tgtgagcgga tgccgggagc
     2221 agacaagccc gccagggcgc gtcagtgcgg gtgtcggggc gcagccctga cccagttcac
     2281 agagcgatag cgatgtgtat actggcttat ctcattttgc atcggtgcag agtggatggg
     2341 aagtgcacca tgtcaggagt aaaaagccgc acagatgcgt aaaaacagcg cacccgataa
     2401 acagcacaca cgcggacact ggcacaggcg cgtaagtgca caatgcttgc atctgtgact
     2461 cttccgcttc ctcgcccact cgctcactac gttcggtcgc tggggctgcg gcgagcggtg
     2521 gggtcacact cgttgtgatg cagatccgtt tttttttttt gcactggagt tatcaggagt
     2581 tgcggtgcac atgggatcgt gcgatatgcc ggatggtgaa aggttggagg gaacggcaac
     2641 aactcacccc tcccagtgaa gggacgggta gtttgccgct ctcgatatgc cggacggagc
     2701 caaggccatc gatcacgggg cgcgtcagtg gaagaacccg cgcctgcgtg acgtatgcgt
     2761 cttcgccgca ccatctcacc cgtcacgcca ccacataccg ggaactgccg ccaaggtcta
     2821 actcaaccaa tctagccgcg ctacatacgg tttttgtggc aactcatcag gtgtccgctg
     2881 tcgatgaaac tctctgcgcc ggtagcggaa ctctcacgcg tgcggtcgcc gtcgtcacag
     2941 ctcccccaac cgcaaacggt ctttgcaaac acgcaagcat ctgcaaaaaa aggatttttt
     3001 tcgggtgtct ggggaggctg tgaggcggaa aaattcttga agaatacaga aggttcatgc
     3061 aaactgaatg aactggtttc tgtcgccgca aacaacagac tccacaggaa cattgaggag
     3121 gtcgaaaacc tcaatgaacc cgaagaagct cgcctaatgg cgggcttttt tcgttctacg
     3181 ctccccgacg aaagatcgca agtgaaataa cgagattttt cacgtcttta cgctcaaaaa
     3241 gcaaaaaaac catgttttcc cctggctgaa tccccaaaaa cgcgtgaaat gcgttttcag
     3301 gatgttgcat agctatgcac aaatacggca cacgatttgt ttcgctcgcc ccagccgtcg
     3361 aactggtcaa aatg
//
