LOCUS       CP052384                4072 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0008 plasmid pD16KP0008-3,
            complete sequence.
ACCESSION   CP052384
VERSION     CP052384.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609906
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,311
            Genes (coding)                    :: 5,197
            CDSs (with protein)               :: 5,197
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 63 of 114
            Pseudo Genes (incomplete)         :: 51 of 114
            Pseudo Genes (internal stop)      :: 26 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0008"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0008-3"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(236..490)
                     /locus_tag="HJX18_27115"
     CDS             complement(236..490)
                     /locus_tag="HJX18_27115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJL10403.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            669..2828
                     /locus_tag="HJX18_27120"
     CDS             669..2828
                     /locus_tag="HJX18_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QJL10405.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(3084..3776)
                     /locus_tag="HJX18_27125"
     CDS             complement(3084..3776)
                     /locus_tag="HJX18_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJL10404.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(492..668)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          669..1027
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(3085..3776)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          583..2825
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgctgaaa acaaaaaacc aataaaaaca atgtgttgca agaaagcaga attgcaagtt
       61 ccgaatactc cccttccaac cctgtggaaa tccagtattc atgcgggttc agcgcatatc
      121 acctaccgaa aaaccgtgcg tatgtgctca acctggcgac ggattcactt ccgcttcctc
      181 gcccactcgc tcgctaacgc tcggtcgctg gggctgcggc gagcggacga tacaatcact
      241 gtcctggcag cgcctccagc tcgttaatca gcggcagtat gcgcgtcaca tcagcctgtc
      301 gtgtcacctc tgccttcagg aactggagta accaggcacg caactgcacc tcctccggct
      361 ttctgacctt tgccagcaat gctgcaccca caacaacctt ctgcgctgtg tccaggctcc
      421 ggctcttcgc cttcaggcgc tgtaatctcg cctcagcatc agcaatcttc tgttcgagcg
      481 ttctgctcat gatgtaactc cgtgcgcgat gaaaaatcgc attttagcgc gtcactggta
      541 gtttaaaaac tgagctggca taatgcgcga caaatcacaa agtgcgcact tatacaatct
      601 ccactccgtt tcgattgtgt gcgccctgcg gggctgaaag aaaactacaa aaaggcatta
      661 cggcagaaat ggcgatttat catctcagca tgaaaattat ctccagaagc agcggctaca
      721 gcgccgtagc ttccgctgcc tatcgttccg gttcactgat gcttgatgag cgtaccggac
      781 tgactcacga ctacacccgc aaaagcggtg tggctgaagc tgtgattctg acccccgcga
      841 cagcccctgc ctggtgtacg aaccgtgccg aactctggaa tgccgttgag aaggccgaat
      901 gccggaagaa ctcacaactt gcgcgggaaa ttgaactcgc tatcccccgt gaactgccgc
      961 aggatgccgc acgggaaacc gttctcgcct ttgtcagaga aaactttgtc agtcagggca
     1021 tgattgctga tgtggcgttc catcacatgg ataaaacaaa tccccatgcg cacatcatgc
     1081 tgaccacgag ggctgtcgga cctgctgggt tcggcggaaa ggtcagggac tggaacgacc
     1141 ggacacatgc cgagacgtgg cgtgcatcat gggctgacca tgcaaacaga gcactggcga
     1201 acgccggtta tcaggaagag atagaccatc gttcatacga gcgacagggg cttgagaaaa
     1261 cgcctggtat tcacctcgga aagtctgcct gtgcgatgga aacacgcggt atcgaaacag
     1321 aacgcggcga acagaatcgt ctgattaaca ggcttaacct ggaaatacag atttcccgca
     1381 ctgaactcag gaacagcggg ctgacagagc ctgcccgccg tgtggctgac cttctgaata
     1441 tcgtgcttcc tgacaacgca acttcgtaca cactacgtga catcatcgaa gcgttgccga
     1501 aggacagtaa tgcggcatgg aaactcacca gtaatgccat gtcgatgatg gcagatatgc
     1561 aggcgacgcg cagacgctgg gaagagctga acaccgaacg aaaggaagcg atgagccatg
     1621 ctgccagcct gaaaaaatca tcgccgtttt cgtccggttt ttcccgtatt cccctgatgc
     1681 aatgggcggc accggaatac cgtagcgaac agggaaaaat ccagtcgctg agccagcaaa
     1741 tggaaaaatt gcgacagcat taccggaagg tggagaaaca ggacattccc gcgagtcaga
     1801 cggcatttga aaaacagtgg cagcagtggg gggcatccgg ccttgcagct ctcagagtcc
     1861 agttgcttaa acgtgaagag gaattgcgcc ggaaggagca ggaggataca gaacgtagtc
     1921 gcgcggagcg acgagcggaa cagctcagga aaaatgacaa tgccgttatg gattcagggg
     1981 tactgagcgc agtcgttacg gggtatggcg aggcccccat gcctggtaac ggggaaatga
     2041 cctgttacct tctgcttcac aaccggaacg gagaatacac gctctggggg aacgagctgg
     2101 agaaataccg caccagaatc tttgagcgtg ttgatttaat gcgtgacagg agtggttata
     2161 tctgtgaccg aagcgaaatc ggtcaacatc caccactgca acgtgtttat tcctctgcga
     2221 cctttgagca acttctgacg caggtttgcc agacatggcc gcaatatacc cgcaatctcc
     2281 gccagccgaa gacatggccg gagtctttct gtcttggaga agaccgccag ccagccatgc
     2341 catcactggc agcaaggaag gtggatttta cgcagggacg tctgctaccg acgctgatgc
     2401 cggtaatgag ttctgtcgac cgggaaaccc gccagcttca gcttttgctg gtcatgggcg
     2461 tggatgactc tctgggcggt gttgtccggc ttaatggtac gctgtatccc gcgtttgccg
     2521 ttccctcagc cgataacagc cagctggtca tcagtgcact gaccgataag gggttacggt
     2581 atgcagggta tggtgttgcc gtaaatcacg atgcagacag ccacatatca cctgcgccgg
     2641 aacttatgga gtttcatctg aaaacccgtg aggcaccgct ttttgctgcc gtcaacacac
     2701 cagaaaaaca gccggatcat ctgttccgct ctctgggttt caatcgtacc tgggatgaat
     2761 ggcggcgcga ggaagatgca agaacacaca ccaccgagag gagacatgat cgcggttggt
     2821 cacagtgaat gtgaacaata ttttgttttg tttaacatcg tgttatgttc gtcgatgttc
     2881 ttctatgttc atcgacaggg atgaatgcaa gaagcacaca cataatgcaa gcatagtttt
     2941 gtttttccgc ccccaaaaat tgggggttct tttttcgctg cattagagcg cttttgacgc
     3001 aatttttcac ttcaatagta ccttcgcact actaacacaa aaaaaacgca gcagggagcg
     3061 ttttaggtgc cttaaaatcg attttagatc gtcatcttga tctgtttgtc ttccttgaag
     3121 cgaaaatgca gggctacaac cgtgcgcccc tttttgatcg tctcaaccgt tacagccagg
     3181 tcgctttttt tgttcagttc gtcaacacag ggttttatta gtattttatt taagtctctg
     3241 aattgtttgt atttattttc aatccctaac attgaacgaa agtcattaag agcaataatt
     3301 ctctctcccg tactgcggaa ttgctggagg aactcgtata ttcttatact gtaactactt
     3361 gataaacctg atatgttttt tataacaacg cgcgtgaatt gccccttcag ttgcgtcaga
     3421 taaggcatta ccgcgtcaga aaaggttatt cgagccttgg cctcaccttt agcgtactgt
     3481 gcccgatact ggatccagcg gaactcctca cgcttctcgt cattcttgag aatgatcgac
     3541 ctgtcccata aacgatcgat cgcttcctgt aactgaacct cggcattttt ccgccccatg
     3601 tcagggaatg aatcaaaata ttccgcagcg gtaatggtca ttgtcttttg caattcagca
     3661 tcagcaccag atttcaatcg actgatacac agcaacagaa aacgttgttc ctgtagggtc
     3721 aacttgtagc tggcctcaac caaagcattc gacttgtagg caatcaagtc cgacatacaa
     3781 agatcccgtt caaatgtggt ggtgctttaa tgtgccccac aaataagaaa tgtggaatat
     3841 ttttttttat atcctcacat ttctgtgaat aacaccttaa attcctccac ctttacctta
     3901 aattcctcca cctaaacacc ttaaattcct ccacctttac cttaaattcc tccacatata
     3961 cctctataac atattgattt tattaatgaa ttttgaccct aaaacaagta ttaaaaacaa
     4021 gatcttaaaa acaagatcta aaaacttaac ggcgagcctg tggataactt gg
//
