LOCUS       CP052378                3511 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0025 plasmid pD16KP0025-3,
            complete sequence.
ACCESSION   CP052378
VERSION     CP052378.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609908
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:58:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,609
            CDSs (total)                      :: 5,485
            Genes (coding)                    :: 5,376
            CDSs (with protein)               :: 5,376
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 50 of 109
            Pseudo Genes (incomplete)         :: 55 of 109
            Pseudo Genes (internal stop)      :: 20 of 109
            Pseudo Genes (multiple problems)  :: 14 of 109
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0025"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0025-3"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(208..519)
                     /locus_tag="HJX20_28020"
     CDS             complement(208..519)
                     /locus_tag="HJX20_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK89449.1"
                     /translation="MRRAEMAYERGGDFLEDARKILNREAIGPRRSRFSIGSAPLTSIT
                     KSDAQISGGETRQDYKDTRRFPLAAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            804..1145
                     /locus_tag="HJX20_28025"
     CDS             804..1145
                     /locus_tag="HJX20_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJK89448.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1084..1842
                     /locus_tag="HJX20_28030"
                     /pseudo=""
     CDS             1084..1842
                     /locus_tag="HJX20_28030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1823..2320
                     /locus_tag="HJX20_28035"
     CDS             1823..2320
                     /locus_tag="HJX20_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJK89445.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            2327..2542
                     /gene="mbeD"
                     /locus_tag="HJX20_28040"
     CDS             2327..2542
                     /gene="mbeD"
                     /locus_tag="HJX20_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJK89446.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mbeD"
     gene            2937..3119
                     /locus_tag="HJX20_28045"
     CDS             2937..3119
                     /locus_tag="HJX20_28045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK89447.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          546..719
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(58..579)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1136..2658
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aactgttctt tcagtgtagc cgcagtcagg cccatacttc aagacctctg aatctctctc
       61 tcgtatatcc cagttaccag tggctgctgc cagtggcgct ttgtcgtgct ttctcgggtt
      121 ggactcaaga tgatagttac cgggtaaggc gcagcagtcg gactgaacgg ggggtttgtg
      181 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
      241 ataaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
      301 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      361 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
      421 acggcttcgc cgcggcccta tcgcttccct gttaagtatc ttcctggcat cttccaggaa
      481 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
      541 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcgccggtgc
      601 cgcctttttt ctcctgccac atgaagcact tcactgacat cctcatccgt gccaacatag
      661 taagccagta tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaaccc
      721 cactgacgcg cctgcggctt gtccaacccc gcgccgaccg ggaaaggctt tcccgtccgt
      781 cacggcgtta tcaggaggcg attctggcag ataaacgcaa caagatgctc acgatgtggg
      841 tgacggaaga cgaacaccgg cggttgcttg agcggtgcga cggaaaacaa ctggcggcgt
      901 ggatgcgcca gacctgcctt gatgagaaac cgacacgcgc cggcaggctt ccctcgattt
      961 cgcccgcgct gctgcgtcag cttgccggca tgggcaataa cctgaaccag atcgcccgga
     1021 aagtgaacgc cggtggtgcc ggtcatgacc gggtgcagat cgtggcagcg ctgatggcga
     1081 tcgatgccgg tctggaacgg ttgcggcatg ccgtactgga aaaagggccg gacgatgatt
     1141 gttaaattcc atccccgggg tcgcggcggg ggcgccggtc ctgtggatta tcttctgggt
     1201 aaagaccggc agcgtgaagg tgccagcgtc ctgcagggga agccggaaga agtccgggag
     1261 ctgattgatg cgtcgccgta tgtgaaaaaa tatacctccg gggtgctgtc attcgcggag
     1321 gcggacctgc cgccgggtca gcgtgaaaaa ctgatggcca gctttgagcg ggttctgatg
     1381 cccggactcg ataaagacca gtacagcatt ctgtgggtgg aacacgcaga caagggtcgt
     1441 cttgagctga attttctgat ccccaatacc gaattactga cggggaaacg cctccagccg
     1501 tattacgatc gtgccgaccg gccgcgcatc gatgcgtggc agacagtggt caacggcagg
     1561 ctggggctac atgatccgaa cgacccggag aaccggcggg cgctggtcac gccttcagga
     1621 ctgccagaaa cgaagcagga agccgcagag acgattacac ggggtttgct gatgttagcc
     1681 tcatcaggag agctgaaaac gcgtgatgac gtcacagggg cgctcacagc ggccggtttt
     1741 gaggtggtca gaaccacaaa aaacagtatc agcattgccg acccggacgg ggggcgaaat
     1801 atccgactga ggggagccat ttatgagcag tcttttaacg gcctcgaggg acttcgagga
     1861 gaaatcgaaa gcacagcagc agcttaccga agagaggctg aaagccgcat tcagcaagca
     1921 cgagaacgtt gtcaaatcgg aactgaacgc aagcgcgaag agaatcaacg acgccatcag
     1981 cgcccacgaa caggggatga acgcggcgat gcagtcgaac cggctgagcg tgctgcgaat
     2041 ggtcgggcgg acgtggctga ccatcaccat ggtgtcaggg ctgctgttcg ccagcctgag
     2101 cggggtgctg tggtaccagg ggaaccggat agcggcgaac ctggcggaaa tcaggcagca
     2161 gagagatacg ctgtcgaaac tgcacatgca gacgtggggc gtgacctatc tggagaaccg
     2221 caacggacgg tttctggtgc tgccggaggg gatgaaagcc gaaacgggcc ggacggtgga
     2281 caacaagacg cggaacgcgg tgaagctggt acgggagtag agcagaatga cggaactgga
     2341 aaaacaattg ctgagcgcgt tagagcagct acagcaggac tactcgagaa ggctggacga
     2401 gtgggagagc gccttcgagg aatggcggaa gatgtctggt cttatgcaac gggagaacgc
     2461 ggcgctgagc gagcgcgtgt cgggcttgag caggcaggtg cagagtttga gcgagcagct
     2521 gcgccggtta tcgacaggct gaatgagatt gagcaacagc gatacgggca aacaacggaa
     2581 aaggaaccag agaagacagc agatctggta ccggaacggc aacgcacatt atctcatggt
     2641 gacagggagt atggagactg ataaacgccc gtctcaggtg gcctgagcgt gacggggatc
     2701 atggccattt tctgcgattt cagcacacag agagtctttt ttaaccgaaa tcaccggcta
     2761 tgcagtgatg tgaataagtg atgcatatgc gtaaaataaa tcaggtgcgt ttctggtggt
     2821 tttcggtgta cttgccacca ccttttgccc gtcagccatc agaaacgccc tgagtgcctc
     2881 cggcgcggtg cgggtaagag atgttatgtt taatgttgat aacaggagta agagtgatgc
     2941 ttacatttat tggtgtagtg gtagtcgccg ttattgcatt taaattattt atgctttttt
     3001 ttagttcatc cgaatctgcc gtcgaacgtg ctacccgtaa atatatggag aaccctacga
     3061 gtgctaacta taaaataatg gtggctacac gcatgagaaa tgagaagaaa agagggtgat
     3121 atatgtttga gtttattggt tttttatttt tacttttggt gtgttatgtc tttatacaaa
     3181 tggttgctgt cagggtgttt cctgaatatg gagtacggaa agcggaggaa cgataccgtc
     3241 gaaatccatc agcagaaaat gaggatctgt tatggagagc tagaagtcgt ataactaaac
     3301 gcaagtaatg tttcctaagt gtgttgatag gcaaacatgg tgggagttac tactctcgga
     3361 gttaatgatc ttcttgtgat cctgtcgttt cagtcgtaat ctcttgctct gaaaacgaaa
     3421 aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccag
     3481 gtaactgggt atacgagacc gcagatacca a
//
