LOCUS       CP052377                5010 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0025 plasmid pD16KP0025-2,
            complete sequence.
ACCESSION   CP052377
VERSION     CP052377.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609908
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:58:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,609
            CDSs (total)                      :: 5,485
            Genes (coding)                    :: 5,376
            CDSs (with protein)               :: 5,376
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 50 of 109
            Pseudo Genes (incomplete)         :: 55 of 109
            Pseudo Genes (internal stop)      :: 20 of 109
            Pseudo Genes (multiple problems)  :: 14 of 109
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0025"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0025-2"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(608..1009)
                     /locus_tag="HJX20_27985"
     CDS             complement(608..1009)
                     /locus_tag="HJX20_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJK89438.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1411..2025
                     /locus_tag="HJX20_27990"
     CDS             1411..2025
                     /locus_tag="HJX20_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="QJK89439.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            complement(2062..2769)
                     /locus_tag="HJX20_27995"
     CDS             complement(2062..2769)
                     /locus_tag="HJX20_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK89440.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(2766..3671)
                     /locus_tag="HJX20_28000"
     CDS             complement(2766..3671)
                     /locus_tag="HJX20_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="QJK89441.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replicase"
     gene            complement(3919..4194)
                     /locus_tag="HJX20_28005"
     CDS             complement(3919..4194)
                     /locus_tag="HJX20_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="QJK89442.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(4175..4420)
                     /locus_tag="HJX20_28010"
     CDS             complement(4175..4420)
                     /locus_tag="HJX20_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="QJK89444.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            4657..4818
                     /locus_tag="HJX20_28015"
     CDS             4657..4818
                     /locus_tag="HJX20_28015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK89443.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
ORIGIN
        1 tgcatgagta tgcgcacaag tgcatagcgc ggaaattgac accattcagc caggggaagt
       61 cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg
      121 ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca gcacgttcag gaatcggttc
      181 gtccacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattccccg
      241 gcgactcttc actagagtcg tacccagagt cgccacgtct aaaaaacaac caaacgggga
      301 gtcggatcta gagtcgggtc gttattaact cattgttttt ataggcctga gtcgttaccg
      361 actctatgac gactcgccgg ggactctatg acgactcgtc atagagtcgg atctatataa
      421 ataaaattta aaaaacgaaa tttcagtaaa gtaagctgct gatattaaat acaaatctgc
      481 aaaattaaaa ttttgcgtgg ggtgtgggta tttttcgtgg tgaggagcgt tcagagatct
      541 gacgattttc gggaaggttc aggaactccc cggaatggcc ggagaggaaa actgaagcat
      601 gacggggtca ggattttttc cagaatttcc accacggcgc cgggggttca gttaattttg
      661 cctggtgatt ttcaagaagg agcatggcct gtttcaggct gtcaatgtgc gaatccttcg
      721 ataaaatcaa tatgttaagg tgttcaatct gctgtttcag ttgacgaatg acatgttcac
      781 tctggtcagc aggcgctgta tcacgctgtt caggtacttg tcgagtaagg tgttcattct
      841 tcggttggtc aaacggtccg aaaacacgta aaagctctga tatatcgacc atttttgtat
      901 ttcgttcacc gctacaagtg gacagttcac ctgttttgat taacctgtgc agcgttgtcc
      961 ggctcttacc cgtcagccgt gcagcctcac taatagacac tctggtcatc agttatcatc
     1021 ctttaataca ttcagtctca aggaaacagg ggccagctta cgacatactg gtccagaagt
     1081 gatgtgtttt tcagaagggc gatgaaacac aggagtgacc gtgcaaaatg caggcaacaa
     1141 atgactttcc gcttcctcgc gcattcgctc actacgttcg gtcataagac tgcggcgagc
     1201 ggtacgtaag aataaagtga ggtgatagct ctcttctcaa ggcgtttagg gaagcaacga
     1261 gcccgcccta aaccagtcat ggcgagacct tgctgaaggg accctatgtg tgctggctga
     1321 gaaaaaaaga gctctctaaa agagagctgt tatctgatat aggttcaagt gcaaaataga
     1381 gtgcacacaa acaaaaaaag gacaataaaa atgggaaagc ataaggtatt cattagctac
     1441 caccatgcga atgatcagta ctacaaaaac gcacttgaaa aaatgaatga ggaacatgaa
     1501 atattcgtga atcgctcagt atcattaggt gatatagatg aagatgaagc cccacaaaaa
     1561 ataagagaga taataagaga tgaatatctt agagatacat cagttcttat ccttcttgtt
     1621 ggaactgaaa caaaaaacag aaaacacgta gattgggaac tttactctag catgagagat
     1681 agtaccatta acaaaaaatc aggaatttta gtcgtcaatc tcccgtcaac aaatactacg
     1741 tacattcgtt caacacatgg aactaatgaa aaatcagaag ttcatcctac cgtaactgaa
     1801 tggttttcta ttgataagcg ctcaacttat gaggaaaggt tcccatatat gccagcaaga
     1861 ataatagaca acttcatgaa gagcgggtct tatatatcag tggtcaactg gaatcaaata
     1921 gaaaacaacc cagaaacact cagaaaatta atagacatga cttataacga tagagaaaaa
     1981 gcaacgtatg atatgtcgag agaaatgaga atgcataatt catagtacta tccaaggagt
     2041 agccttggct actcctttaa tttaccagtt ccacttatct gtattgattc caaacttagg
     2101 cttcacatca ttaacaaaga accggttaac atctttaaaa ccttttaaaa tacgctcata
     2161 atcataatac tgcatttgta ttttatttat gctggttgca tgaaatctca tgacatcacc
     2221 gctaacccta tcaacaacct catctttgtc tgatatgtac ttataccaac gcttgtagtt
     2281 gttctggtag ttagttagaa atctatttaa caccatgagc atctcattgc tgaaatcata
     2341 aatctcacga tcttttttag ggtttagtat tttcatttca tcacgaataa attggtaagt
     2401 ctgataatat gatgaaataa cctcatcaaa ggcatcaacg ttttttctta atttgaaaat
     2461 tgctcttttt gaatctaggt gattttttac actctttttg taaagtgtag cactctcatt
     2521 gaaaagtata ttcactccac caaggctaag tttctcaacc ttaaccgaat actttttaag
     2581 taagtagagg gataccatca gccaaataaa aacagatatc aaaagaatag aaagggcgcc
     2641 agttacaaga ggtctattct tactaaaaac tgtgtaaaca gacggaatgt aattagatat
     2701 atcaacagta cacatcacgg caataaatgc taaaaaaact atagtccaaa gagttacgaa
     2761 ggttttcatc ttatttcacc atttttcttg atataaaaat acttccgcct gcttattccc
     2821 atagctaccc aaggttttat ttcctctagg cttcctttcc tccttgactg tcctgacttt
     2881 ttccctctct ttgattgaat ttcagaggaa tgagtaatat caacaaattt actgaattct
     2941 tcaggtgaaa atttatttga tgtccattta gcaatgctct tagctatccc tcttacctca
     3001 ttttcatcca gaggccgtgc aaattgcagg ttgtatgcgc aagcgcgttc gtagcaggct
     3061 tgcagccact gctcatactc aggccagccc tgacggatgg cgcgatatgc ccacttacgg
     3121 gttttgtcga acaacgtgca gttacggcct aagccgtagt cggcaacgat ttccttgtca
     3181 ttccctgtgt tcagatcgag aaagtcggcc agccagtcca gagtgtagag cttcggctgc
     3241 cacacggcga ttttccagtg gttgtggttc ggatttttgc agatgagccc cgaatatcca
     3301 gcgtcagcct ctaatttttt acgtagcgca ttctccacgg aggcggcgta gcgtaacggt
     3361 tttattttgc cgtccggcgc ggtgcgtatc gagttttcca gcgcgtacag caggtgcgcg
     3421 tgtccgtttt cggggttagt tatggtcagc gtcggtgcag gcgcgttgcg gtcgctccag
     3481 tcgatggagg aaccggcgcg gtcaacgtca aatcccagcc agaacatggc gtgaggctga
     3541 ttgaactgga tatatttcgc cagaatagcg cgttccttac cggcgatacg gacaccaaag
     3601 tgcagatcgt ccgaaaagta cggcttgtgt ggtaagcgtt cgttgaaaag ttcgagtgcg
     3661 gcgttatgca atgtgccctg cgcttatggc gggctgttgt ctggcacggt tcatgtgcta
     3721 atatcctttc aaggtttcga cctagccctg gttatcatgc gatccgccaa gatatgacat
     3781 gatttccggg gctttgtttt ttctgaggct aacgttacac cgactttgcc tttgtgtcga
     3841 tctgacagcg acttacccta acatccgttg tacgccttgc atgaaaagcc cgcatcaagc
     3901 gggctatatc gacttttgct atttgtcacc atagtgataa cgggctgcgt agatgatgat
     3961 ttctccgttc tggattgaat agaccagacg gtggctctga tcaatcctgc gggagtagag
     4021 tgccggatct ttgtggtgcc gcagccgttc gggttttcct atacctgtca gcggatcagc
     4081 ctcgatgctg agcaacagtt gccggatacg ctcaaacttc tttttgtcag tatcacgcca
     4141 gtagttaagg tcatcagcgc cattcctagt gaacttaata ttccacttcg acaaattcgc
     4201 cgcgctccgc ctgtttcatt gactcattga tatgctctgc gttcgccgga ttgctgaaca
     4261 gatagaccgt ttcgattagc gcctcatact ctcccgcatc gatcataaca atatcaggcg
     4321 catcacggcg catgacacgt accggttcag catcatcagt gacgcgcttc atcgtctcgg
     4381 caaaatgttt acgggcatca gtaaaattga ttgcatgcat gttgttaacc cccatttgtt
     4441 ggggcttagc atagaacatg tacgcaaata agtacataaa gatatttccc taaaaaccca
     4501 caaccaaatc agctcaaaat gtgaactcaa tcgaataacc acaaaaacaa caagtcaata
     4561 cattgaagca taatttcaag caattacatt tatgaagtgc ggcacaattt cgtaccgcta
     4621 ggagcttagc tcctaaaacg agcaaaggaa aagtaaatgg caaaacattg gtctgggcaa
     4681 gaatgtccaa aaacaggtac ttacggtcaa tatcatgata ccgacaactc atacgctggt
     4741 tctgaatatg atcgccatgt gagcaagggt gataagttcc ctccgagcaa aaacaaccac
     4801 cacttccaag aaaaataaaa tatcttcaga agtctaaact aaaagccctc tggggctttt
     4861 tttgtacctg ccattcactt gatttaccat aacatccgtt atcagcaccg cctgagatgc
     4921 cctcagggcg tttctgacgg gaaaccggta aacaaggcaa caaaccaccc gaaaacccgc
     4981 cagaaacgcg caggctctgt ttttacggca
//
