LOCUS       CP052371                4167 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0087 plasmid pD16KP0087-2,
            complete sequence.
ACCESSION   CP052371
VERSION     CP052371.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609910
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:39:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,295
            CDSs (total)                      :: 5,171
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 54 of 112
            Pseudo Genes (incomplete)         :: 52 of 112
            Pseudo Genes (internal stop)      :: 17 of 112
            Pseudo Genes (multiple problems)  :: 9 of 112
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0087"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0087-2"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.8 N 127.7 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4025..4167,1..100))
                     /locus_tag="HJX22_26445"
     CDS             complement(join(4025..4167,1..100))
                     /locus_tag="HJX22_26445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK57881.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(466..1098)
                     /locus_tag="HJX22_26450"
     CDS             complement(466..1098)
                     /locus_tag="HJX22_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK57877.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="hypothetical protein"
     gene            complement(1098..2213)
                     /locus_tag="HJX22_26455"
     CDS             complement(1098..2213)
                     /locus_tag="HJX22_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QJK57878.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     gene            complement(2558..2671)
                     /locus_tag="HJX22_26460"
                     /pseudo=""
     CDS             complement(2558..2671)
                     /locus_tag="HJX22_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2696..3130)
                     /locus_tag="HJX22_26465"
                     /pseudo=""
     CDS             complement(2696..3130)
                     /locus_tag="HJX22_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            3087..3278
                     /locus_tag="HJX22_26470"
     CDS             3087..3278
                     /locus_tag="HJX22_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK57879.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3278..3679
                     /locus_tag="HJX22_26475"
     CDS             3278..3679
                     /locus_tag="HJX22_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJK57880.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2559..2966)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 cacgcaataa attgcgtccc cgttcaccgt tttcggagga gaagaacgcc ggaaacagga
       61 cttcccctgg ctgaatggtg tgaatttccg cgctatgcac ttgcgcgcat actcatgcat
      121 gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt
      181 accggtttcc cgtcagaaac gtcctgagtc cgttttagcg gtgcgtacaa ttatgatttt
      241 atgttaaatt gttttaagtg aatagagaga atataactta agggagcatc ttgtgctggg
      301 tcttttacat tagatctgca caatcattgt tttggcatct ggtgatgtct ttaggacgtt
      361 tgagattgct gataactaaa gtgtatgatg ctttctatac gttatccggc gtttaataac
      421 accctggtga taagccagcc cgtgaagggc tggcttgatt tggatttata tttttgaaat
      481 aaaatctttt atttcatcaa agcaattgtc tgctgtgttt attattccat ttttcatgga
      541 tattcctatt cttgagtcgg attctctatt gcagctaaga tagacttgta ccttagcttt
      601 tttttcgttt gttggataag tgtactcttt gtcactcgct gagatgtcat ctttattatt
      661 tttaattttt tcgaaaaatt ctattatgta ttcatataac gctcgagtgc ttgcgtgttc
      721 aattatcaga tcttcaatgg caccttgctt gtcttttcct ggcattataa agaaagaaat
      781 ttttgtattg ccattcatag ctatctcgtt atgcgagctg ggggaggaga tttgtagttc
      841 tcgcaatgtg gcaattattg atctttctgt gtcagaaaat gatgtgtcag catctaatat
      901 aataaaaata ctttcttttt catcgaaaga atcaatgttt ttgaatgctc taagtgcttg
      961 ttttaatttt gattttccat ttattttttc gacaaaaaca tcggtaatgc cttgtatttc
     1021 aagtaggttg catagaaaat gaaaatcatc atcaccctca acgaaaatga tctttttgct
     1081 attatttatg tctttcatta tcttacctcc cagtcgttct caactgagta ggagaaatca
     1141 tccatattga attgtacggc tgttggtttt agggagttct tttctcttcc tagtctaata
     1201 taagcgatat cttcattttt gctatctatt tcgtttattg ctcgtatcac atcttgacta
     1261 tgagttgttg caaatatttg attgttgttt tgttttgcag atgaaattaa tgattttatt
     1321 atgtctttaa ttactgagta atgtattccg ttctcgattt catcaataag aataattgag
     1381 ttttcctgta ccagcactga gctgataaaa gataatgctc gactgatacc ctcgccaagc
     1441 attgatattt catttagctc actaaatcca atgtctaaat atatttcctt attgttacct
     1501 atcgcagata ttgctatatc tacaattctg tcatctattt tttttaagtt ttctagtatg
     1561 tcttttttcc ttttttgtgt tagtaggcta gatacatttg taattgttgc ctctttgttt
     1621 atctttctgg atgttgttat tattgttgct gtcttaaatt tataattaat cccatccctt
     1681 tcgtttttta tttcggattt tatttctacg ccatcttctt tcaattgcat agagaaaagc
     1741 ttattggaaa ccctgttttt cgcattgggt gtctcggtca catttatttt taaagagttt
     1801 actgtggcct gagaaccgat agtttgacga ggtatgtttt ggttagatag tacggtgtca
     1861 acgtttagag acaaacttga ttctaatctc tgatttgttt ctgtttcata tgtttgtgtt
     1921 acttcagagc gttcatctcg aatgactatg gacatctttt gtgatgtgtc catatcatga
     1981 aagtaactaa cccaaaatgg cttgtttttg tttatttctg ccatttcttt tctgaatgct
     2041 tgaatgttta acaaaacagc cgggtttttt acatcataaa aacaaaaaat agcatccagt
     2101 atagatgttt tcccgtagtt gtttttccca gctatgatat ttaaagtctt taattgcttt
     2161 agatatagct cattgaagca tttgaagttc tttaaactta tttcctttat catgatagcc
     2221 tctgttcgtg tttttgttat atgccatacg atttgttagt accttaataa aatgtacagc
     2281 tttcattgtg attagctaga ctttatgtca aagattaact ttgattgctt gtgttttaat
     2341 cgattttttt tactttcttc ttgagattat ctggcagcag ccgtaagctt ccggctgtga
     2401 acgaaaaaaa ccgccttggc gggcggtttt ttcgaaaggt tcagtagagt tggcgctcta
     2461 tgaaccgagg taactggctt aaagggagcg cagtcaccaa aactgttcgt ttagtttagc
     2521 tttaactggc gtataacttc aagactaact ccctaaatca gttaccagtg gctgctgcca
     2581 gtggcgcttt gtcgtgtctt tccgggttgg actcaagacg atagttaccg gattaggcgc
     2641 agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc
     2701 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
     2761 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc catcaggggg aaacgcctgg
     2821 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat tccgtgatgc
     2881 tcgtcagggg ggcggagcct atggaaaaac ggccgctgtg cggcctttcc ttctttcctc
     2941 catgcgatta ctcggttttc tcgagcgttt ctacgcatgc tcctgccgct cgccgcagcc
     3001 gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagaggag atccctacac
     3061 tgaccactga cgctctgagg cctcttatga cgaaacaaca gaaaacagcg ctgaacatgg
     3121 ctaaattcat ccagaatcag tctctgctcc tgctggagaa gcttaacgag cttgatctcg
     3181 atgtcgaggc cgacctttgt gagaaactcc acgatgatgc ggagcatctt ttccgtacac
     3241 tgtcctcgag actggatgca ttacaggatg gtaactgatg gccagagtgt ctattagtga
     3301 ggctgcccga ctgacgggta agagtagaac aacactgcac aggttaatta aagcaggtga
     3361 cctgtccacc tgtagcggtg agcgcaatgc aaaaatggta gatacttcag agcttttacg
     3421 tgtctttggc ccctttgagc aaccgaaggg tgaacaccat tctggacagg tctctggaca
     3481 gcgtgataca gcctctgctg accagagtga acaggtgatc cgtcatctga aacaggagat
     3541 tgaacacctg aacaccttac ttttgtccaa ggattcgcac attgacagcc tgaaacaggc
     3601 catgctcctt cttgaaaatc accaggcaaa attaactgaa tccccggcgc catggtggaa
     3661 attctggaaa aaatcctgac cccgtcatgc ttcagttttc ctctccggcc atcccaggga
     3721 gttcctgaac cttcccaaaa atcgtcagat ctctgaacgc tcctcaccac taaaaatacc
     3781 cacaccccac gcaaaattaa aattttgcag attccttatt tacaacaatg ggttaatgaa
     3841 aaataaatgt gcggtgatgt gttacaaagt gcagatccaa gtcgtggacg actctatggc
     3901 gaatcgctga cgaattgccc acgattcata tgtgaatcac gctttattat catattgtta
     3961 tgatgaatcg actcttgatt caattcatca ctttgggtgt tttttgctca tggtgactct
     4021 tgattcaatt cacgattcga atcgccggtg aatcgtgtgg tgagtcgtcc ggcgacttgc
     4081 cataacgtga ttgccggccg tcgacgaacc cgattcctga aggtgctgaa aaccggtttt
     4141 ccggctgcgc cggcaacgct tcacggc
//
