LOCUS       CP052368                3511 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0109 plasmid pD16KP0109-3,
            complete sequence.
ACCESSION   CP052368
VERSION     CP052368.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609911
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:13:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,615
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,391
            CDSs (with protein)               :: 5,391
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 51 of 100
            Pseudo Genes (internal stop)      :: 21 of 100
            Pseudo Genes (multiple problems)  :: 13 of 100
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0109"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0109-3"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            155..466
                     /locus_tag="HJX23_28050"
     CDS             155..466
                     /locus_tag="HJX23_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO27515.1"
                     /translation="MRRAEMAYERGGDFLEDARKILNREAIGPRRSRFSIGSAPLTSIT
                     KSDAQISGGETRQDYKDTRRFPLAAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            complement(1066..1248)
                     /locus_tag="HJX23_28055"
     CDS             complement(1066..1248)
                     /locus_tag="HJX23_28055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO27511.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(1643..1858)
                     /gene="mbeD"
                     /locus_tag="HJX23_28060"
     CDS             complement(1643..1858)
                     /gene="mbeD"
                     /locus_tag="HJX23_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJO27512.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mbeD"
     gene            complement(1865..2362)
                     /locus_tag="HJX23_28065"
     CDS             complement(1865..2362)
                     /locus_tag="HJX23_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJO27513.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            complement(2343..3101)
                     /locus_tag="HJX23_28070"
                     /pseudo=""
     CDS             complement(2343..3101)
                     /locus_tag="HJX23_28070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3040..3381)
                     /locus_tag="HJX23_28075"
     CDS             complement(3040..3381)
                     /locus_tag="HJX23_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJO27514.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2..129)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.54e-61"
     Typing          96..617
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1528..3050)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3047..3360)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
ORIGIN
        1 gtatactggc ttactatgtt ggcacggatg aggatgtcag tgaagtgctt catgtggcag
       61 gagaaaaaag gcggcaccgg cgcgtcagca gaatatgtga tacaggatat attccgcttc
      121 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
      181 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag cgatagggcc
      241 gcggcgaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
      301 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
      361 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
      421 tggccgcgtt tatctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
      481 gctggactgt atgcacaaac cccccgttca gtccgactgc tgcgccttac ccggtaacta
      541 tcatcttgag tccaacccga gaaagcacga caaagcgcca ctggcagcag ccactggtaa
      601 ctgggatata cgagagagag attcagaggt cttgaagtat gggcctgact gcggctacac
      661 tgaaagaaca gttttggtat ctgcggtctc gtatacccag ttacctggtt aagcagttcc
      721 ccaactgact taaccttcga tcaaaccacc tccccaggtg gttttttcgt tttcagagca
      781 agagattacg actgaaacga caggatcaca agaagatcat taactccgag agtagtaact
      841 cccaccatgt ttgcctatca acacacttag gaaacattac ttgcgtttag ttatacgact
      901 tctagctctc cataacagat cctcattttc tgctgatgga tttcgacggt atcgttcctc
      961 cgctttccgt actccatatt caggaaacac cctgacagca accatttgta taaagacata
     1021 acacaccaaa agtaaaaata aaaaaccaat aaactcaaac atatatcacc ctcttttctt
     1081 ctcatttctc atgcgtgtag ccaccattat tttatagtta gcactcgtag ggttctccat
     1141 atatttacgg gtagcacgtt cgacggcaga ttcggatgaa ctaaaaaaaa gcataaataa
     1201 tttaaatgca ataacggcga ctaccactac accaataaat gtaagcatca ctcttactcc
     1261 tgttatcaac attaaacata acatctctta cccgcaccgc gccggaggca ctcagggcgt
     1321 ttctgatggc tgacgggcaa aaggtggtgg caagtacacc gaaaaccacc agaaacgcac
     1381 ctgatttatt ttacgcatat gcatcactta ttcacatcac tgcatagccg gtgatttcgg
     1441 ttaaaaaaga ctctctgtgt gctgaaatcg cagaaaatgg ccatgatccc cgtcacgctc
     1501 aggccacctg agacgggcgt ttatcagtct ccatactccc tgtcaccatg agataatgtg
     1561 cgttgccgtt ccggtaccag atctgctgtc ttctctggtt ccttttccgt tgtttgcccg
     1621 tatcgctgtt gctcaatctc attcagcctg tcgataaccg gcgcagctgc tcgctcaaac
     1681 tctgcacctg cctgctcaag cccgacacgc gctcgctcag cgccgcgttc tcccgttgca
     1741 taagaccaga catcttccgc cattcctcga aggcgctctc ccactcgtcc agccttctcg
     1801 agtagtcctg ctgtagctgc tctaacgcgc tcagcaattg tttttccagt tccgtcattc
     1861 tgctctactc ccgtaccagc ttcaccgcgt tccgcgtctt gttgtccacc gtccggcccg
     1921 tttcggcttt catcccctcc ggcagcacca gaaaccgtcc gttgcggttc tccagatagg
     1981 tcacgcccca cgtctgcatg tgcagtttcg acagcgtatc tctctgctgc ctgatttccg
     2041 ccaggttcgc cgctatccgg ttcccctggt accacagcac cccgctcagg ctggcgaaca
     2101 gcagccctga caccatggtg atggtcagcc acgtccgccc gaccattcgc agcacgctca
     2161 gccggttcga ctgcatcgcc gcgttcatcc cctgttcgtg ggcgctgatg gcgtcgttga
     2221 ttctcttcgc gcttgcgttc agttccgatt tgacaacgtt ctcgtgcttg ctgaatgcgg
     2281 ctttcagcct ctcttcggta agctgctgct gtgctttcga tttctcctcg aagtccctcg
     2341 aggccgttaa aagactgctc ataaatggct cccctcagtc ggatatttcg ccccccgtcc
     2401 gggtcggcaa tgctgatact gttttttgtg gttctgacca cctcaaaacc ggccgctgtg
     2461 agcgcccctg tgacgtcatc acgcgttttc agctctcctg atgaggctaa catcagcaaa
     2521 ccccgtgtaa tcgtctctgc ggcttcctgc ttcgtttctg gcagtcctga aggcgtgacc
     2581 agcgcccgcc ggttctccgg gtcgttcgga tcatgtagcc ccagcctgcc gttgaccact
     2641 gtctgccacg catcgatgcg cggccggtcg gcacgatcgt aatacggctg gaggcgtttc
     2701 cccgtcagta attcggtatt ggggatcaga aaattcagct caagacgacc cttgtctgcg
     2761 tgttccaccc acagaatgct gtactggtct ttatcgagtc cgggcatcag aacccgctca
     2821 aagctggcca tcagtttttc acgctgaccc ggcggcaggt ccgcctccgc gaatgacagc
     2881 accccggagg tatatttttt cacatacggc gacgcatcaa tcagctcccg gacttcttcc
     2941 ggcttcccct gcaggacgct ggcaccttca cgctgccggt ctttacccag aagataatcc
     3001 acaggaccgg cgcccccgcc gcgaccccgg ggatggaatt taacaatcat cgtccggccc
     3061 tttttccagt acggcatgcc gcaaccgttc cagaccggca tcgatcgcca tcagcgctgc
     3121 cacgatctgc acccggtcat gaccggcacc accggcgttc actttccggg cgatctggtt
     3181 caggttattg cccatgccgg caagctgacg cagcagcgcg ggcgaaatcg agggaagcct
     3241 gccggcgcgt gtcggtttct catcaaggca ggtctggcgc atccacgccg ccagttgttt
     3301 tccgtcgcac cgctcaagca accgccggtg ttcgtcttcc gtcacccaca tcgtgagcat
     3361 cttgttgcgt ttatctgcca gaatcgcctc ctgataacgc cgtgacggac gggaaagcct
     3421 ttcccggtcg gcgcggggtt ggacaagccg caggcgcgtc agtggggttt cggggcgcag
     3481 ccctgaacca gtcacgtagc gctagcggag t
//
