LOCUS       CP052367                5010 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0109 plasmid pD16KP0109-2,
            complete sequence.
ACCESSION   CP052367
VERSION     CP052367.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609911
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:13:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,615
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,391
            CDSs (with protein)               :: 5,391
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 51 of 100
            Pseudo Genes (internal stop)      :: 21 of 100
            Pseudo Genes (multiple problems)  :: 13 of 100
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0109"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0109-2"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4852..5010,1..3))
                     /locus_tag="HJX23_28015"
     CDS             complement(join(4852..5010,1..3))
                     /locus_tag="HJX23_28015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO27509.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            240..485
                     /locus_tag="HJX23_28020"
     CDS             240..485
                     /locus_tag="HJX23_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="QJO27510.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            466..741
                     /locus_tag="HJX23_28025"
     CDS             466..741
                     /locus_tag="HJX23_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="QJO27504.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            989..1894
                     /locus_tag="HJX23_28030"
     CDS             989..1894
                     /locus_tag="HJX23_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="QJO27505.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replicase"
     gene            1891..2598
                     /locus_tag="HJX23_28035"
     CDS             1891..2598
                     /locus_tag="HJX23_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO27506.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(2635..3249)
                     /locus_tag="HJX23_28040"
     CDS             complement(2635..3249)
                     /locus_tag="HJX23_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="QJO27507.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            3651..4052
                     /locus_tag="HJX23_28045"
     CDS             3651..4052
                     /locus_tag="HJX23_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO27508.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 catttacttt tcctttgctc gttttaggag ctaagctcct agcggtacga aattgtgccg
       61 cacttcataa atgtaattgc ttgaaattat gcttcaatgt attgacttgt tgtttttgtg
      121 gttattcgat tgagttcaca ttttgagctg atttggttgt gggtttttag ggaaatatct
      181 ttatgtactt atttgcgtac atgttctatg ctaagcccca acaaatgggg gttaacaaca
      241 tgcatgcaat caattttact gatgcccgta aacattttgc cgagacgatg aagcgcgtca
      301 ctgatgatgc tgaaccggta cgtgtcatgc gccgtgatgc gcctgatatt gttatgatcg
      361 atgcgggaga gtatgaggcg ctaatcgaaa cggtctatct gttcagcaat ccggcgaacg
      421 cagagcatat caatgagtca atgaaacagg cggagcgcgg cgaatttgtc gaagtggaat
      481 attaagttca ctaggaatgg cgctgatgac cttaactact ggcgtgatac tgacaaaaag
      541 aagtttgagc gtatccggca actgttgctc agcatcgagg ctgatccgct gacaggtata
      601 ggaaaacccg aacggctgcg gcaccacaaa gatccggcac tctactcccg caggattgat
      661 cagagccacc gtctggtcta ttcaatccag aacggagaaa tcatcatcta cgcagcccgt
      721 tatcactatg gtgacaaata gcaaaagtcg atatagcccg cttgatgcgg gcttttcatg
      781 caaggcgtac aacggatgtt agggtaagtc gctgtcagat cgacacaaag gcaaagtcgg
      841 tgtaacgtta gcctcagaaa aaacaaagcc ccggaaatca tgtcatatct tggcggatcg
      901 catgataacc agggctaggt cgaaaccttg aaaggatatt agcacatgaa ccgtgccaga
      961 caacagcccg ccataagcgc agggcacatt gcataacgcc gcactcgaac ttttcaacga
     1021 acgcttacca cacaagccgt acttttcgga cgatctgcac tttggtgtcc gtatcgccgg
     1081 taaggaacgc gctattctgg cgaaatatat ccagttcaat cagcctcacg ccatgttctg
     1141 gctgggattt gacgttgacc gcgccggttc ctccatcgac tggagcgacc gcaacgcgcc
     1201 tgcaccgacg ctgaccataa ctaaccccga aaacggacac gcgcacctgc tgtacgcgct
     1261 ggaaaactcg atacgcaccg cgccggacgg caaaataaaa ccgttacgct acgccgcctc
     1321 cgtggagaat gcgctacgta aaaaattaga ggctgacgct ggatattcgg ggctcatctg
     1381 caaaaatccg aaccacaacc actggaaaat cgccgtgtgg cagccgaagc tctacactct
     1441 ggactggctg gccgactttc tcgatctgaa cacagggaat gacaaggaaa tcgttgccga
     1501 ctacggctta ggccgtaact gcacgttgtt cgacaaaacc cgtaagtggg catatcgcgc
     1561 catccgtcag ggctggcctg agtatgagca gtggctgcaa gcctgctacg aacgcgcttg
     1621 cgcatacaac ctgcaatttg cacggcctct ggatgaaaat gaggtaagag ggatagctaa
     1681 gagcattgct aaatggacat caaataaatt ttcacctgaa gaattcagta aatttgttga
     1741 tattactcat tcctctgaaa ttcaatcaaa gagagggaaa aagtcaggac agtcaaggag
     1801 gaaaggaagc ctagaggaaa taaaaccttg ggtagctatg ggaataagca ggcggaagta
     1861 tttttatatc aagaaaaatg gtgaaataag atgaaaacct tcgtaactct ttggactata
     1921 gtttttttag catttattgc cgtgatgtgt actgttgata tatctaatta cattccgtct
     1981 gtttacacag tttttagtaa gaatagacct cttgtaactg gcgccctttc tattcttttg
     2041 atatctgttt ttatttggct gatggtatcc ctctacttac ttaaaaagta ttcggttaag
     2101 gttgagaaac ttagccttgg tggagtgaat atacttttca atgagagtgc tacactttac
     2161 aaaaagagtg taaaaaatca cctagattca aaaagagcaa ttttcaaatt aagaaaaaac
     2221 gttgatgcct ttgatgaggt tatttcatca tattatcaga cttaccaatt tattcgtgat
     2281 gaaatgaaaa tactaaaccc taaaaaagat cgtgagattt atgatttcag caatgagatg
     2341 ctcatggtgt taaatagatt tctaactaac taccagaaca actacaagcg ttggtataag
     2401 tacatatcag acaaagatga ggttgttgat agggttagcg gtgatgtcat gagatttcat
     2461 gcaaccagca taaataaaat acaaatgcag tattatgatt atgagcgtat tttaaaaggt
     2521 tttaaagatg ttaaccggtt ctttgttaat gatgtgaagc ctaagtttgg aatcaataca
     2581 gataagtgga actggtaaat taaaggagta gccaaggcta ctccttggat agtactatga
     2641 attatgcatt ctcatttctc tcgacatatc atacgttgct ttttctctat cgttataagt
     2701 catgtctatt aattttctga gtgtttctgg gttgttttct atttgattcc agttgaccac
     2761 tgatatataa gacccgctct tcatgaagtt gtctattatt cttgctggca tatatgggaa
     2821 cctttcctca taagttgagc gcttatcaat agaaaaccat tcagttacgg taggatgaac
     2881 ttctgatttt tcattagttc catgtgttga acgaatgtac gtagtatttg ttgacgggag
     2941 attgacgact aaaattcctg attttttgtt aatggtacta tctctcatgc tagagtaaag
     3001 ttcccaatct acgtgttttc tgttttttgt ttcagttcca acaagaagga taagaactga
     3061 tgtatctcta agatattcat ctcttattat ctctcttatt ttttgtgggg cttcatcttc
     3121 atctatatca cctaatgata ctgagcgatt cacgaatatt tcatgttcct cattcatttt
     3181 ttcaagtgcg tttttgtagt actgatcatt cgcatggtgg tagctaatga ataccttatg
     3241 ctttcccatt tttattgtcc tttttttgtt tgtgtgcact ctattttgca cttgaaccta
     3301 tatcagataa cagctctctt ttagagagct ctttttttct cagccagcac acatagggtc
     3361 ccttcagcaa ggtctcgcca tgactggttt agggcgggct cgttgcttcc ctaaacgcct
     3421 tgagaagaga gctatcacct cactttattc ttacgtaccg ctcgccgcag tcttatgacc
     3481 gaacgtagtg agcgaatgcg cgaggaagcg gaaagtcatt tgttgcctgc attttgcacg
     3541 gtcactcctg tgtttcatcg cccttctgaa aaacacatca cttctggacc agtatgtcgt
     3601 aagctggccc ctgtttcctt gagactgaat gtattaaagg atgataactg atgaccagag
     3661 tgtctattag tgaggctgca cggctgacgg gtaagagccg gacaacgctg cacaggttaa
     3721 tcaaaacagg tgaactgtcc acttgtagcg gtgaacgaaa tacaaaaatg gtcgatatat
     3781 cagagctttt acgtgttttc ggaccgtttg accaaccgaa gaatgaacac cttactcgac
     3841 aagtacctga acagcgtgat acagcgcctg ctgaccagag tgaacatgtc attcgtcaac
     3901 tgaaacagca gattgaacac cttaacatat tgattttatc gaaggattcg cacattgaca
     3961 gcctgaaaca ggccatgctc cttcttgaaa atcaccaggc aaaattaact gaacccccgg
     4021 cgccgtggtg gaaattctgg aaaaaatcct gaccccgtca tgcttcagtt ttcctctccg
     4081 gccattccgg ggagttcctg aaccttcccg aaaatcgtca gatctctgaa cgctcctcac
     4141 cacgaaaaat acccacaccc cacgcaaaat tttaattttg cagatttgta tttaatatca
     4201 gcagcttact ttactgaaat ttcgtttttt aaattttatt tatatagatc cgactctatg
     4261 acgagtcgtc atagagtccc cggcgagtcg tcatagagtc ggtaacgact caggcctata
     4321 aaaacaatga gttaataacg acccgactct agatccgact ccccgtttgg ttgtttttta
     4381 gacgtggcga ctctgggtac gactctagtg aagagtcgcc ggggaatcgt gtggtgagtc
     4441 gtccggcgac ttgccataac gtgattgccg gccgtggacg aaccgattcc tgaacgtgct
     4501 gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc
     4561 cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga cttcccctgg ctgaatggtg
     4621 tcaatttccg cgctatgcac ttgtgcgcat actcatgcat gccgtaaaaa cagagcctgc
     4681 gcgtttctgg cgggttttcg ggtggtttgt tgccttgttt accggtttcc cgtcagaaac
     4741 gccctgaggg catctcaggc ggtgctgata acggatgtta tggtaaatca agtgaatggc
     4801 aggtacaaaa aaagccccag agggctttta gtttagactt ctgaagatat tttatttttc
     4861 ttggaagtgg tggttgtttt tgctcggagg gaacttatca cccttgctca catggcgatc
     4921 atattcagaa ccagcgtatg agttgtcggt atcatgatat tgaccgtaag tacctgtttt
     4981 tggacattct tgcccagacc aatgttttgc
//
