LOCUS       CP052360                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0144 plasmid pD16KP0144-4,
            complete sequence.
ACCESSION   CP052360
VERSION     CP052360.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609913
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:06:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,610
            CDSs (total)                      :: 5,484
            Genes (coding)                    :: 5,312
            CDSs (with protein)               :: 5,312
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 82 of 172
            Pseudo Genes (incomplete)         :: 82 of 172
            Pseudo Genes (internal stop)      :: 33 of 172
            Pseudo Genes (multiple problems)  :: 23 of 172
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0144"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD16KP0144-4"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(7403..9294,1..73)
                     /locus_tag="HJX25_27960"
     CDS             join(7403..9294,1..73)
                     /locus_tag="HJX25_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJJ93989.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            73..804
                     /locus_tag="HJX25_27965"
     CDS             73..804
                     /locus_tag="HJX25_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJJ93979.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            811..1341
                     /locus_tag="HJX25_27970"
     CDS             811..1341
                     /locus_tag="HJX25_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ93980.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1369..1548)
                     /locus_tag="HJX25_27975"
     CDS             complement(1369..1548)
                     /locus_tag="HJX25_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ93981.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1617..1994
                     /locus_tag="HJX25_27980"
     CDS             1617..1994
                     /locus_tag="HJX25_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJJ93982.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(2056..2355)
                     /locus_tag="HJX25_27985"
     CDS             complement(2056..2355)
                     /locus_tag="HJX25_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJJ93983.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2345..2608)
                     /locus_tag="HJX25_27990"
     CDS             complement(2345..2608)
                     /locus_tag="HJX25_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJJ93984.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(2914..3333)
                     /gene="exc2"
                     /locus_tag="HJX25_27995"
     CDS             complement(2914..3333)
                     /gene="exc2"
                     /locus_tag="HJX25_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJJ93985.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            3736..5421
                     /locus_tag="HJX25_28000"
     CDS             3736..5421
                     /locus_tag="HJX25_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ93986.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            5431..5688
                     /locus_tag="HJX25_28005"
     CDS             5431..5688
                     /locus_tag="HJX25_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ93987.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            5773..5922
                     /locus_tag="HJX25_28010"
     CDS             5773..5922
                     /locus_tag="HJX25_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJJ93988.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6026..6604)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          74..801
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 cctggcggag ttccggcccc cggccgacgg cggcgggccg aaagcggatg atgattttgc
       61 ggggatttac tgatggcgct ggagctgtac aacgtcagcc acgcgaaacg acaggcgcgg
      121 aacgcggaga aaacccggct gaccctgcgc tggttgcggg aagagctgtg ttccacggcg
      181 gagctggttg cgcggcggct ggggattgcg gcggtgcagc cggtctaccg cttcctcgac
      241 agcctggtgg cgaaagggct gctggtccgg accaaatatc cggtggacgg ccgtcaggtc
      301 agcgtgtggg ggctgacgcc gcatggcgtg gcgttctcgt tcgacgagga cgaaccgctg
      361 gcggatgtta ttccgtttca gccgtcaagg gtgtcggcag cacatctgcc gcaccggctg
      421 gccgtgcaat ccctgcgcct tgatatggaa gccagagggg ccaccggctg gcgcattctg
      481 caccgtctga gtcttaaagg catgaaagtg ccggacgccc tggcggagct ggacggtaaa
      541 acggtggcgt ttgaggtgga gcgcaccgtc aagagccgtc gccggtatca ggaagtggtg
      601 tccgggtatc tgttcaaccg caaagctaac ggcatcgatg aaatctggta catctgcccg
      661 gaccgggcga cgcaggtcag ggtgcagcgg gcaatcctgt cggtggatga aatcgtgaac
      721 ccacagaccg gcgaggcccg caaaacggca gagctggatc gtgaacggtt gtttgcgtgc
      781 tttaaattta tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaagac
      841 gcggaggacc ggaacggcga gcgctgcatc atgccgacca gcgagcatct ggaagatatg
      901 gtggtgctcg cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat
      961 ctggcgatga tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc
     1021 acgtactttt tcactggtaa aaatccgcaa tgggaacgga acaaacccac aggaatgaag
     1081 ctgtgtgatt acctctatcg caaggtggag cggttcgcca tcgagaacgg tgtgcaggtg
     1141 gttgatgccg ggaagtgctc accggagcag attaacgcga tgattgacgt ggtgagtgag
     1201 cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac
     1261 agccgctatt ttaacgtgcc ggtacctgaa ctgccggagg acaaacctgc ccgccggtgg
     1321 tggccgttcg gcaaaaaata aatgccagca ccggccatgc cggtgctgtt attcctcccc
     1381 gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgtagcc gctcgcacat
     1441 gtcggccggg gtatccatat ccagctcatt cagtttatcg agcaatagca gcgactgctc
     1501 cttaataaat ttagccatat tgagggctgt tttttcctgt tttgtcatga ttctttcctt
     1561 ccgggcatgg tgtgcgcctg tcaggtatac tgtctcaaaa atagcggggt gagacaatgg
     1621 cgtgggtgac ggtgagacag gcgacggaac tgacgggtaa agcccgaagt tccctgtatc
     1681 gggatatggc gaaaggccgc gtatcatacc gtacggaagc tgatggcggc cgggtggtgg
     1741 ataccagcga gcttatccgg gtgtacggtg agttaagaca ggatgagact cacgagcggg
     1801 acgagttgag actgtcgggt gagactgaaa aaatggcctc cgacgcgttg atcgcggaga
     1861 taaaagcact cagggaggag gtggccgggc tacggcagga aatgcagtcc atgagactgc
     1921 tggaacacaa accggagaca gcagagaccc cggccggaac accgcgccgg tggtggccgt
     1981 ggggaaaacg ctgacgcgct gtgcttgtcc aaccggcgac ggccacgcaa tgaattgcgt
     2041 ccccgtcacc ggttattaga gaacaatatg gggattctgg cgctggccga ccacggccag
     2101 aacccggact tttctggtct ggtcatcaac atcatagagg acatgctgtc ccatcagact
     2161 gatccggcga acgtcctcgc catagcgtga gtccacttca tagagtcgcg ggaagtcggc
     2221 cagtttgtcc gccgcagcga gaacccggtc ggtaacgttc tgcgcatccc acagggatga
     2281 attttccgcg atatagtcaa acagcgtcgt tacatcctcg atggctccct gcttccattc
     2341 cacgtcatac ggcatcgcgc agttcctgat aacggccatg caggcgctgg cggagttgtt
     2401 ccgtggtcat acccggacgc gtgtcagcac gggcggcggt cactttggcg cgaacctgat
     2461 cttcatactc tgcctgggcc agttcttcgt tagccagtcg ctgcaacagc gcagctttgt
     2521 ccgggccacc ttccggcagt atgcggggca ggctttcagc cagttcccga agaaagttgg
     2581 cgttttcgta gtgtgtagtg ttgctcattg cggagcctcc cgggttaatg cggcaatatg
     2641 aaggaagttt agcagggggc ggtctgaaag aatatggtcg tgtcagtcag gctgatgaag
     2701 cagaaagtcg gtaaaaaagg gcttacggca tccattttac gtcaaaacat atgccatgca
     2761 gttgcgtgca taggcatgca ttaggataaa atttatcggg cgcgtttccg gcagttttcc
     2821 gggggggttg ttgcctgttt tatcccgtag ccgccggaaa cgccctgagc ccgtctgagc
     2881 ggtgcgcgta atgacgcgtt atggtaaatc cacttactgt ctgccttcat agccatcgag
     2941 ataaacagct gaaatggtgt cagccagtag ccttgatttt ttcggtgtga ggtctttgct
     3001 gcggtaatcg gtacccgcaa attgtgttgt gccttggagt gagtttaaaa attcctgact
     3061 gtgaaactgc tgaatgcgtt gctgcacatc actccgtgat tttccggtcg ctttatcctt
     3121 tgcgcccata tcccagaact gccggaaaaa aggcaccatc attcgtgttg tgtcagcttt
     3181 ctgggtggaa aaattgggat caaaaccgtc atctgaagca tatacgtaat gccgggtatg
     3241 ctgttccacg ctgccctttg gagcacaggc agtgagagtt gctgtcgctg ttatcatggc
     3301 gataagacac agtttgctgc cggaacgtct catgagcgtc tccttacctt taattctttt
     3361 ttctcattga gttatatatc cgcaggctat cttatcatca gtttctgacc gtgttcagtg
     3421 accgaacaac ccgaaccaga ccccccgttt cttttcctgt cccggagggg gctttctggt
     3481 atctcatata atgatttcag taaaacatac tgaggccgga gaacggcgcc gtttgtgctg
     3541 gtttttgttg catctggagg ggattgcgca tggtgtgagt ggctgttttt ttgatcattt
     3601 tcacaaaaac gcaaatttac agacttgaca tgaaaaacaa ggcagcgtaa tttttatact
     3661 gtatataaaa ccagtggtta tatgtacagt attttatttt agtttattgt tttaaaagtc
     3721 aaagaggaaa agtatatgag tggcggagac ggacgaggtc cgggtaattc aggtctggga
     3781 cacaatggtg gtcaggccag tgggaatgtg aacggtacat caggtaaagg tggcccttca
     3841 tcaggtggtg gtacggatcc aaacagcggg ccgggctggg gtacgacgca tacacctaac
     3901 ggggatattc ataactataa cccgggggag tttggtaacg gcgggagtaa acccggtggt
     3961 aatggcggta acagcggcaa tcatagcggg agctctggtg gcggacagtc ttcggccacc
     4021 gcgatggcct tcggtctgcc tgccctggcc actccggggg ctgaaggact ggctttatcc
     4081 gtttccggcg atgcgttgtc ggccgctgtt gctgatgtgc tggctgccct gaaaggtccg
     4141 tttaagtttg gtctgtgggg gattgcgatt tacggagtgc tgccttctga gatagcaaaa
     4201 gatgatccga aaatgatgtc aaaaatcgtg acgtcattgc cggcagacac ggtaacggag
     4261 acgccggtca gctccctgcc gctggaccag gcgacggtca gcgtcactaa acgtgtggcg
     4321 gatattgtga aggacgagcg gcagcatatt gcggttgtca ccggccggcc aatgagtgtt
     4381 cctgtggtgg atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt
     4441 ctcccgtctc tgcaggtgag cgtacctaaa ggtgttccgg ccgcgaaagc cccgccaaaa
     4501 ggcattattg ctgaaaaagg tgattcacgt ccggctggtt ttacggccgg tggtaactcc
     4561 cgtgaggccg ttattcgttt cccgaaagag agcggacaga aaccggtcta tgtgtcggtg
     4621 acggatgttc ttactccggc acaggtaaaa cagcgtctgg aggaagaaaa gcgtcgccag
     4681 caggcatggg acgctgctca cccggaagag gggctgaaaa gagagtatga taaagcgaaa
     4741 gctgagctgg atgctgaaga taaaaatatt gcgaccttaa acagtcgcat tgcatcgaca
     4801 gagaaggcga tccccggtgc aagggctgct gttcaggaag ccgataaaaa ggtaaaagag
     4861 gcagaggcaa ataaagatga ttttgtgact tataacccgc ctcatgaata tggctccggg
     4921 tggcaggatc aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa
     4981 gctgcccaga catctttaaa cgaaatgaat gaatccttat ccagggataa ggctgcgctt
     5041 tccggggcga tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataaa
     5101 ttaaatgagg aaaagaaaaa acctcgtaag ggaactaaag attacggcca tgattatttt
     5161 cctgatccca agactgaaga tattaagggg ttgggagagt tgaaagaggg taaacctaaa
     5221 acccctaaac aaggtggtgg tggtaagcgg gcccgatggt atggtgataa aaagcgtaaa
     5281 atttatgaat gggattccca gcacggtgag cttgaagggt accgcgccag tgatggcgaa
     5341 cacctcgggg cattcgatcc aaaaacgggt aagcaggtta aagggccgga tccaaaacga
     5401 aacattaaaa aatatcttta agaggtaaat atggggctta aattacatat tcattggttt
     5461 gataagaaaa ccgaagagtt taaaggtggt gaatactcaa aagacttcgg tgatgatggt
     5521 tctgtcattg aaagtctggg gatgccttta aaggataata ttaataatgg ttggtttgat
     5581 gttgaaaaat catgggtttc gatattacag ccacacttta aaaatgtaat cgatattagt
     5641 aaatttgatt actttgtatc ctttgtttac cgggatggta actggtaagc aaaagttatc
     5701 aaggatgatt tgaaatacag acttccggtg ttcagggatg aataccgggg tttttaaata
     5761 cagggaaggt atatgaaaaa ggtaaaggtt gtttttcttt ttatattgat tttttcaggc
     5821 tttcttctgg tcgcatgtca ggcaaactat atccgggatg ttcagggtgg aacggtggca
     5881 ccatcgtcct cctctgaact gacggggatc gcggttcagt agaaaagatc aaaggatctt
     5941 cttgagatcc tttttttctg cgcgtaatct tttgccctgt aaacgaaaaa accacctggg
     6001 gaggtggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccgtgg taactggctt
     6061 tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg cgcacacttc
     6121 aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg
     6181 ctgccagtgg cgttttgcgt gcttttccgg gttggactca agtgaacagt taccggataa
     6241 ggcgcagcag tcgggctgaa cggggggttc ttgcttacag cccagcttgg agcgaacgac
     6301 ctacaccgag ccgagatacc agagtgtgag ctatgagaaa gcgccacact tcccgcaagg
     6361 gagaaaggcg gaacaggtat ccgggaaacg gcagggttgg aacaggagag cgcaagaggg
     6421 agcgattcat cggaaacggt gatgatcttt aagtcctgtc gggtttcgcc actcctgact
     6481 gattcatggt tgagccaccg gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa
     6541 ctcagtcagg agggcggagc ctatggaaaa acgccacctg cgtggccttg ccgttttgcc
     6601 tcacatgtta gtcccctgct tattcacgga atctgtgggt aactttgcat gtgtccgcag
     6661 cgcccgccgc agtctcacgg ccggagcgta gcgaccgagt gagcgaggag gcgcaatttc
     6721 cccggcacct acaagacgga caccggacgc gctgatgtgg catattgtcc cctgattaag
     6781 tctttatatt ccctgataat aaattaccag gataggtgaa tcatatcggt ttttcgctga
     6841 acattttacg ttaacctatc tctgttgctt ttttctcctt acggagaaat acaggtcatc
     6901 cccgcgaggg cgtggctgac ctcaggggga tatcccctgc ttacgctggc tggacgccac
     6961 cgacgcacgg tctctccccc tctttctggc tccgtccggc tggacgccgg ccacgccaga
     7021 aattcacccg gcccccgtca ggctcacgcc tgaccaccca caaaaatcca cggtgctgga
     7081 cgcacctggc gaagcagcaa agctgctcct gattttgcgg gtggtcagcc gctcgctatg
     7141 gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc gccttttcct
     7201 ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg catggggcgg
     7261 aaagaggctg gcctccgtgg tgaaagctga acgcaatcac cacggacgtg accgccgccg
     7321 ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc tgcacatcac gccggcaaac
     7381 catcgtctga aggagagaga acatgtttaa tacggattcg cttgcctgga agtggcacat
     7441 tccggagctg gcccggcaga tttatccgtg ggtcagctgg ctgtaccagg agtattacgg
     7501 tctggccggg gctttcctag ttatcggcct gtttttcggt ctggctgaac tttgggccgg
     7561 acggatgcgt tttgtggggc tgagtcataa ggagcgacgg gagtatgccg atcgctataa
     7621 gcgcgataat aaacggccag cgccattttc ggtgctggcc tcgctggatt cgcccgggcg
     7681 gatggtcagg gcacgtcgac atgtctggaa ctacgcgtac tggctgaccg gcgcggccgg
     7741 tctggccctt ctgggggggc tggttgtcgc cgtgtttatc gagagctggg gagaaaccgt
     7801 cctgcgtccg gacctctacg caaacgcaga ttattacctg ctgcagccgg ctaacctgtt
     7861 cgggattgcc tggtgcgcgg gcgggctggc tgtcgggatt gtgttggcgc ggctgctggc
     7921 gttcctgctg cttgacggtc attttgcggc tgctgacgag gcggtgaacg cgaaactgaa
     7981 tcaggagagc cggcgcagca gccagcggac aggagagatg accgacgtgc gccacctgca
     8041 tttcggggag ccggtaccgg ttaatgcgct ggccgatttt tcctcggagc aggcccggca
     8101 ggagcaggcg gtgtttctgg gtaaggatga gcagggcaag ccggtgctgg ttccgcgtga
     8161 tacctggcgc aagaccaaca ttcagatcct cggcctgccc gggagcggca agagcgtgat
     8221 ggccaccaac gcgctcattc gctgcgtacg cgatttcggg gacgcggtgg tgtacttcga
     8281 ccccaaaggg gacgcatggg cgccgcacgt cttccgggcg cactgtccgg acttcaccct
     8341 gctggatctg cggccgggga aaccggcgca gctgaatctg ttccgcgatc tcgaccagta
     8401 cgcgctgaaa aacctgctga ttgccgggct taacctgggc gagaccggcg acgtggcgga
     8461 ccactaccgc atcagcgagc agaaagcggc gaagctgatt gcggagcagt ttccgcaggg
     8521 ggcaaacatt cagcaggtgc tggcggcggc gtacgcgctg ccggaggccc tgaaaaagga
     8581 cgtgaagggg ttgatcacta ggctggaaaa cgtcgctgac ctgagcgtcc tgcagacgga
     8641 cagtggcatc gatgtggcgg ggattatcaa cggcggcggc tgcctgtacg ttatcggctc
     8701 gatggacgac gaggcggtga tccgtgtgca aaaaatgctg tttgcccgct gcgcgcaaat
     8761 catcattgcg cgggacgagt tccgggagtg gccacacgtc agcgtgatgc tcgatgaaat
     8821 taaatatctg ctgtcgaagt atgtactgaa cgcactgggg acgctgcgca gccgggactg
     8881 caatctgttg ctcgcgcacc agtcgctggg cgatttcggg cagtgcgggc aggatctgcc
     8941 cgctgaattt gtgaaaacca cggtactgga taacacgccg atccgctggt tttaccgggc
     9001 ggccagcctg gagtccgcgc agtgggcggc ggggcagacc ggggaaatca gggtggacgt
     9061 ggagcgccgc cgtgccagcc gggaggcggg gaacgtggag catatcagcg gggacagctt
     9121 catccagaag gaggcgcggc cgctgtttga cgtgaacacc ctgcaacacc tgccggacgg
     9181 gttcgcggtc atgaccgggc tgggtgtggc ccggatggcg ttcagcagtc cgctgcgggt
     9241 tgagcgccgg gaaatcccgt tgcagcgttt cccggtgctg gcgaaaaccg atcc
//
