LOCUS       CP052355                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D16KP0146 plasmid pD17KP0032-3,
            complete sequence.
ACCESSION   CP052355
VERSION     CP052355.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609914
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:52:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,661
            CDSs (total)                      :: 5,535
            Genes (coding)                    :: 5,377
            CDSs (with protein)               :: 5,377
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 84 of 158
            Pseudo Genes (incomplete)         :: 67 of 158
            Pseudo Genes (internal stop)      :: 23 of 158
            Pseudo Genes (multiple problems)  :: 16 of 158
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D16KP0146"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD17KP0032-3"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(8631..9294,1..1022)
                     /locus_tag="HJX26_28255"
     CDS             join(8631..9294,1..1022)
                     /locus_tag="HJX26_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK52812.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            1032..1289
                     /locus_tag="HJX26_28260"
     CDS             1032..1289
                     /locus_tag="HJX26_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK52813.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            1374..1523
                     /locus_tag="HJX26_28265"
     CDS             1374..1523
                     /locus_tag="HJX26_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJK52814.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3004..4968
                     /locus_tag="HJX26_28270"
     CDS             3004..4968
                     /locus_tag="HJX26_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJK52815.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            4968..5699
                     /locus_tag="HJX26_28275"
     CDS             4968..5699
                     /locus_tag="HJX26_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJK52816.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            5706..6236
                     /locus_tag="HJX26_28280"
     CDS             5706..6236
                     /locus_tag="HJX26_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK52817.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6264..6443)
                     /locus_tag="HJX26_28285"
     CDS             complement(6264..6443)
                     /locus_tag="HJX26_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK52818.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6512..6889
                     /locus_tag="HJX26_28290"
     CDS             6512..6889
                     /locus_tag="HJX26_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJK52819.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(6951..7250)
                     /locus_tag="HJX26_28295"
     CDS             complement(6951..7250)
                     /locus_tag="HJX26_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJK52820.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7240..7503)
                     /locus_tag="HJX26_28300"
     CDS             complement(7240..7503)
                     /locus_tag="HJX26_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJK52821.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(7809..8228)
                     /gene="exc2"
                     /locus_tag="HJX26_28305"
     CDS             complement(7809..8228)
                     /gene="exc2"
                     /locus_tag="HJX26_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJK52822.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1627..2205)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4969..5696
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga
       61 gcgtacctaa aggtgttccg gccgcgaaag ccccgccaaa aggcattatt gctgaaaaag
      121 gtgattcacg tccggctggt tttacggccg gtggtaactc ccgtgaggcc gttattcgtt
      181 tcccgaaaga gagcggacag aaaccggtct atgtgtcggt gacggatgtt cttactccgg
      241 cacaggtaaa acagcgtctg gaggaagaaa agcgtcgcca gcaggcatgg gacgctgctc
      301 acccggaaga ggggctgaaa agagagtatg ataaagcgaa agctgagctg gatgctgaag
      361 ataaaaatat tgcgacctta aacagtcgca ttgcatcgac agagaaggcg atccccggtg
      421 caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg
      481 attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct
      541 atcttgataa ggatattcag aatcagaatg agaaattaaa agctgcccag acatctttaa
      601 acgaaatgaa tgaatcctta tccagggata aggctgcgct ttccggggcg atggagagcc
      661 ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa attaaatgag gaaaagaaaa
      721 aacctcgtaa gggaactaaa gattacggcc atgattattt tcctgatccc aagactgaag
      781 atattaaggg gttgggagag ttgaaagagg gtaaacctaa aacccctaaa caaggtggtg
      841 gtggtaagcg ggcccgatgg tatggtgata aaaagcgtaa aatttatgaa tgggattccc
      901 agcacggtga gcttgaaggg taccgcgcca gtgatggcga acacctcggg gcattcgatc
      961 caaaaacggg taagcaggtt aaagggccgg atccaaaacg aaacattaaa aaatatcttt
     1021 aagaggtaaa tatggggctt aaattacata ttcattggtt tgataagaaa accgaagagt
     1081 ttaaaggtgg tgaatactca aaagacttcg gtgatgatgg ttctgtcatt gaaagtctgg
     1141 ggatgccttt aaaggataat attaataatg gttggtttga tgttgaaaaa tcatgggttt
     1201 cgatattaca gccacacttt aaaaatgtaa tcgatattag taaatttgat tactttgtat
     1261 cctttgttta ccgggatggt aactggtaag caaaagttat caaggatgat ttgaaataca
     1321 gacttccggt gttcagggat gaataccggg gtttttaaat acagggaagg tatatgaaaa
     1381 aggtaaaggt tgtttttctt tttatattga ttttttcagg ctttcttctg gtcgcatgtc
     1441 aggcaaacta tatccgggat gttcagggtg gaacggtggc accatcgtcc tcctctgaac
     1501 tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct
     1561 gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg
     1621 ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca
     1681 aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta
     1741 gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg
     1801 tgcttttccg ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga
     1861 acggggggtt cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac
     1921 cagagtgtga gctatgagaa agcgccacac ttcccgcaag ggagaaaggc ggaacaggta
     1981 tccgggaaac ggcagggttg gaacaggaga gcgcaagagg gagcgattca tcggaaacgg
     2041 tgatgatctt taagtcctgt cgggtttcgc cactcctgac tgattcatgg ttgagccacc
     2101 ggctcccaca gatgcaccga aaaagcgtct gtttatgtga actcagtcag gagggcggag
     2161 cctatggaaa aacgccacct gcgtggcctt gccgttttgc ctcacatgtt agtcccctgc
     2221 ttattcacgg aatctgtggg taactttgca tgtgtccgca gcgcccgccg cagtctcacg
     2281 gccggagcgt agcgaccgag tgagcgagga ggcgcaattt ccccggcacc tacaagacgg
     2341 acaccggacg cgctgatgtg gcatattgtc ccctgattaa gtctttatat tccctgataa
     2401 taaattacca ggataggtga atcatatcgg tttttcgctg aacattttac gttaacctat
     2461 ctctgttgct tttttctcct tacggagaaa tacaggtcat ccccgcgagg gcgtggctga
     2521 cctcaggggg atatcccctg cttacgctgg ctggacgcca ccgacgcacg gtctctcccc
     2581 ctctttctgg ctccgtccgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc
     2641 aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg acgcacctgg cgaagcagca
     2701 aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc
     2761 ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc
     2821 tcgttgttcc tccggggctg cgccttttcc gcatggggcg gaaagaggct ggcctccgtg
     2881 gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc
     2941 cgcggcttgt gcagcggggg ctgcacatca cgccggcaaa ccatcgtctg aaggagagag
     3001 aacatgttta atacggattc gcttgcctgg aagtggcaca ttccggagct ggcccggcag
     3061 atttatccgt gggtcagctg gctgtaccag gagtattacg gtctggccgg ggctttccta
     3121 gttatcggcc tgtttttcgg tctggctgaa ctttgggccg gacggatgcg ttttgtgggg
     3181 ctgagtcata aggagcgacg ggagtatgcc gatcgctata agcgcgataa taaacggcca
     3241 gcgccatttt cggtgctggc ctcgctggat tcgcccgggc ggatggtcag ggcacgtcga
     3301 catgtctgga actacgcgta ctggctgacc ggcgcggccg gtctggccct tctggggggg
     3361 ctggttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgtcc ggacctctac
     3421 gcaaacgcag attattacct gctgcagccg gctaacctgt tcgggattgc ctggtgcgcg
     3481 ggcgggctgg ctgtcgggat tgtgttggcg cggctgctgg cgttcctgct gcttgacggt
     3541 cattttgcgg ctgctgacga ggcggtgaac gcgaaactga atcaggagag ccggcgcagc
     3601 agccagcgga caggagagat gaccgacgtg cgccacctgc atttcgggga gccggtaccg
     3661 gttaatgcgc tggccgattt ttcctcggag caggcccggc aggagcaggc ggtgtttctg
     3721 ggtaaggatg agcagggcaa gccggtgctg gttccgcgtg atacctggcg caagaccaac
     3781 attcagatcc tcggcctgcc cgggagcggc aagagcgtga tggccaccaa cgcgctcatt
     3841 cgctgcgtac gcgatttcgg ggacgcggtg gtgtacttcg accccaaagg ggacgcatgg
     3901 gcgccgcacg tcttccgggc gcactgtccg gacttcaccc tgctggatct gcggccgggg
     3961 aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgcgctgaa aaacctgctg
     4021 attgccgggc ttaacctggg cgagaccggc gacgtggcgg accactaccg catcagcgag
     4081 cagaaagcgg cgaagctgat tgcggagcag tttccgcagg gggcaaacat tcagcaggtg
     4141 ctggcggcgg cgtacgcgct gccggaggcc ctgaaaaagg acgtgaaggg gttgatcact
     4201 aggctggaaa acgtcgctga cctgagcgtc ctgcagacgg acagtggcat cgatgtggcg
     4261 gggattatca acggcggcgg ctgcctgtac gttatcggct cgatggacga cgaggcggtg
     4321 atccgtgtgc aaaaaatgct gtttgcccgc tgcgcgcaaa tcatcattgc gcgggacgag
     4381 ttccgggagt ggccacacgt cagcgtgatg ctcgatgaaa ttaaatatct gctgtcgaag
     4441 tatgtactga acgcactggg gacgctgcgc agccgggact gcaatctgtt gctcgcgcac
     4501 cagtcgctgg gcgatttcgg gcagtgcggg caggatctgc ccgctgaatt tgtgaaaacc
     4561 acggtactgg ataacacgcc gatccgctgg ttttaccggg cggccagcct ggagtccgcg
     4621 cagtgggcgg cggggcagac cggggaaatc agggtggacg tggagcgccg ccgtgccagc
     4681 cgggaggcgg ggaacgtgga gcatatcagc ggggacagct tcatccagaa ggaggcgcgg
     4741 ccgctgtttg acgtgaacac cctgcaacac ctgccggacg ggttcgcggt catgaccggg
     4801 ctgggtgtgg cccggatggc gttcagcagt ccgctgcggg ttgagcgccg ggaaatcccg
     4861 ttgcagcgtt tcccggtgct ggcgaaaacc gatcccctgg cggagttccg gcccccggcc
     4921 gacggcggcg ggccgaaagc ggatgatgat tttgcgggga tttactgatg gcgctggagc
     4981 tgtacaacgt cagccacgcg aaacgacagg cgcggaacgc ggagaaaacc cggctgaccc
     5041 tgcgctggtt gcgggaagag ctgtgttcca cggcggagct ggttgcgcgg cggctgggga
     5101 ttgcggcggt gcagccggtc taccgcttcc tcgacagcct ggtggcgaaa gggctgctgg
     5161 tccggaccaa atatccggtg gacggccgtc aggtcagcgt gtgggggctg acgccgcatg
     5221 gcgtggcgtt ctcgttcgac gaggacgaac cgctggcgga tgttattccg tttcagccgt
     5281 caagggtgtc ggcagcacat ctgccgcacc ggctggccgt gcaatccctg cgccttgata
     5341 tggaagccag aggggccacc ggctggcgca ttctgcaccg tctgagtctt aaaggcatga
     5401 aagtgccgga cgccctggcg gagctggacg gtaaaacggt ggcgtttgag gtggagcgca
     5461 ccgtcaagag ccgtcgccgg tatcaggaag tggtgtccgg gtatctgttc aaccgcaaag
     5521 ctaacggcat cgatgaaatc tggtacatct gcccggaccg ggcgacgcag gtcagggtgc
     5581 agcgggcaat cctgtcggtg gatgaaatcg tgaacccaca gaccggcgag gcccgcaaaa
     5641 cggcagagct ggatcgtgaa cggttgtttg cgtgctttaa atttatgaca acggagtgag
     5701 ggattatggc gctgtacatg gtatttattg aagacgcgga ggaccggaac ggcgagcgct
     5761 gcatcatgcc gaccagcgag catctggaag atatggtggt gctcgcctgg cagaccgggg
     5821 cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa tccatcggga
     5881 tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcact ggtaaaaatc
     5941 cgcaatggga acggaacaaa cccacaggaa tgaagctgtg tgattacctc tatcgcaagg
     6001 tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg
     6061 agcagattaa cgcgatgatt gacgtggtga gtgagcggca cggggaaagc gcggtgaacg
     6121 agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtac
     6181 ctgaactgcc ggaggacaaa cctgcccgcc ggtggtggcc gttcggcaaa aaataaatgc
     6241 cagcaccggc catgccggtg ctgttattcc tccccgagct tatcccgcat cacctgccag
     6301 agtgtttccg catcctcgtg tagccgctcg cacatgtcgg ccggggtatc catatccagc
     6361 tcattcagtt tatcgagcaa tagcagcgac tgctccttaa taaatttagc catattgagg
     6421 gctgtttttt cctgttttgt catgattctt tccttccggg catggtgtgc gcctgtcagg
     6481 tatactgtct caaaaatagc ggggtgagac aatggcgtgg gtgacggtga gacaggcgac
     6541 ggaactgacg ggtaaagccc gaagttccct gtatcgggat atggcgaaag gccgcgtatc
     6601 ataccgtacg gaagctgatg gcggccgggt ggtggatacc agcgagctta tccgggtgta
     6661 cggtgagtta agacaggatg agactcacga gcgggacgag ttgagactgt cgggtgagac
     6721 tgaaaaaatg gcctccgacg cgttgatcgc ggagataaaa gcactcaggg aggaggtggc
     6781 cgggctacgg caggaaatgc agtccatgag actgctggaa cacaaaccgg agacagcaga
     6841 gaccccggcc ggaacaccgc gccggtggtg gccgtgggga aaacgctgac gcgctgtgct
     6901 tgtccaaccg gcgacggcca cgcaatgaat tgcgtccccg tcaccggtta ttagagaaca
     6961 atatggggat tctggcgctg gccgaccacg gccagaaccc ggacttttct ggtctggtca
     7021 tcaacatcat agaggacatg ctgtcccatc agactgatcc ggcgaacgtc ctcgccatag
     7081 cgtgagtcca cttcatagag tcgcgggaag tcggccagtt tgtccgccgc agcgagaacc
     7141 cggtcggtaa cgttctgcgc atcccacagg gatgaatttt ccgcgatata gtcaaacagc
     7201 gtcgttacat cctcgatggc tccctgcttc cattccacgt catacggcat cgcgcagttc
     7261 ctgataacgg ccatgcaggc gctggcggag ttgttccgtg gtcatacccg gacgcgtgtc
     7321 agcacgggcg gcggtcactt tggcgcgaac ctgatcttca tactctgcct gggccagttc
     7381 ttcgttagcc agtcgctgca acagcgcagc tttgtccggg ccaccttccg gcagtatgcg
     7441 gggcaggctt tcagccagtt cccgaagaaa gttggcgttt tcgtagtgtg tagtgttgct
     7501 cattgcggag cctcccgggt taatgcggca atatgaagga agtttagcag ggggcggtct
     7561 gaaagaatat ggtcgtgtca gtcaggctga tgaagcagaa agtcggtaaa aaagggctta
     7621 cggcatccat tttacgtcaa aacatatgcc atgcagttgc gtgcataggc atgcattagg
     7681 ataaaattta tcgggcgcgt ttccggcagt tttccggggg ggttgttgcc tgttttatcc
     7741 cgtagccgcc ggaaacgccc tgagcccgtc tgagcggtgc gcgtaatgac gcgttatggt
     7801 aaatccactt actgtctgcc ttcatagcca tcgagataaa cagctgaaat ggtgtcagcc
     7861 agtagccttg attttttcgg tgtgaggtct ttgctgcggt aatcggtacc cgcaaattgt
     7921 gttgtgcctt ggagtgagtt taaaaattcc tgactgtgaa actgctgaat gcgttgctgc
     7981 acatcactcc gtgattttcc ggtcgcttta tcctttgcgc ccatatccca gaactgccgg
     8041 aaaaaaggca ccatcattcg tgttgtgtca gctttctggg tggaaaaatt gggatcaaaa
     8101 ccgtcatctg aagcatatac gtaatgccgg gtatgctgtt ccacgctgcc ctttggagca
     8161 caggcagtga gagttgctgt cgctgttatc atggcgataa gacacagttt gctgccggaa
     8221 cgtctcatga gcgtctcctt acctttaatt cttttttctc attgagttat atatccgcag
     8281 gctatcttat catcagtttc tgaccgtgtt cagtgaccga acaacccgaa ccagaccccc
     8341 cgtttctttt cctgtcccgg agggggcttt ctggtatctc atataatgat ttcagtaaaa
     8401 catactgagg ccggagaacg gcgccgtttg tgctggtttt tgttgcatct ggaggggatt
     8461 gcgcatggtg tgagtggctg tttttttgat cattttcaca aaaacgcaaa tttacagact
     8521 tgacatgaaa aacaaggcag cgtaattttt atactgtata taaaaccagt ggttatatgt
     8581 acagtatttt attttagttt attgttttaa aagtcaaaga ggaaaagtat atgagtggcg
     8641 gagacggacg aggtccgggt aattcaggtc tgggacacaa tggtggtcag gccagtggga
     8701 atgtgaacgg tacatcaggt aaaggtggcc cttcatcagg tggtggtacg gatccaaaca
     8761 gcgggccggg ctggggtacg acgcatacac ctaacgggga tattcataac tataacccgg
     8821 gggagtttgg taacggcggg agtaaacccg gtggtaatgg cggtaacagc ggcaatcata
     8881 gcgggagctc tggtggcgga cagtcttcgg ccaccgcgat ggccttcggt ctgcctgccc
     8941 tggccactcc gggggctgaa ggactggctt tatccgtttc cggcgatgcg ttgtcggccg
     9001 ctgttgctga tgtgctggct gccctgaaag gtccgtttaa gtttggtctg tgggggattg
     9061 cgatttacgg agtgctgcct tctgagatag caaaagatga tccgaaaatg atgtcaaaaa
     9121 tcgtgacgtc attgccggca gacacggtaa cggagacgcc ggtcagctcc ctgccgctgg
     9181 accaggcgac ggtcagcgtc actaaacgtg tggcggatat tgtgaaggac gagcggcagc
     9241 atattgcggt tgtcaccggc cggccaatga gtgttcctgt ggtggatgcg aaac
//
