LOCUS       CP052342                3223 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D17KP0018 plasmid pD17KP0018-5,
            complete sequence.
ACCESSION   CP052342
VERSION     CP052342.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609916
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:48:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,860
            CDSs (total)                      :: 5,736
            Genes (coding)                    :: 5,599
            CDSs (with protein)               :: 5,599
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 63 of 137
            Pseudo Genes (incomplete)         :: 64 of 137
            Pseudo Genes (internal stop)      :: 30 of 137
            Pseudo Genes (multiple problems)  :: 19 of 137
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D17KP0018"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD17KP0018-5"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            407..751
                     /locus_tag="HJX28_29265"
     CDS             407..751
                     /locus_tag="HJX28_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJK26126.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            690..1454
                     /locus_tag="HJX28_29270"
                     /pseudo=""
     CDS             690..1454
                     /locus_tag="HJX28_29270"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1429..1914
                     /locus_tag="HJX28_29275"
     CDS             1429..1914
                     /locus_tag="HJX28_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJK26127.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="mobilization protein"
     gene            1918..2133
                     /gene="mbeD"
                     /locus_tag="HJX28_29280"
     CDS             1918..2133
                     /gene="mbeD"
                     /locus_tag="HJX28_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJK26128.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="mbeD"
     gene            2301..2633
                     /locus_tag="HJX28_29285"
     CDS             2301..2633
                     /locus_tag="HJX28_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="QJK26129.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             2302..2633
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          141..332
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(2668..3190)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.143"
                     /coverage="99.0"
                     /evalue="8.31e-121"
     Typing          742..2231
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggggggcgg agcctatgga aaaacggcaa ctccgcatcc ttttcttggc ctttcctgct
       61 tcgagcggca tttgcttatc ccgtaatctg tgagctggta acgcccgccg cagtctcacg
      121 gcaggagcgt agcgaccgag tgagcgagga ggcgtcattt catcaccacc tgcattttgc
      181 acttacgccc ggcagccgca tttttcccct gtcacatgaa gcactttcat aaattgccac
      241 tccgtcataa catgtttagc cagtatacac tccgctagcg ctacgtgact ggttcagggc
      301 tgcgccccga aacccgctaa aaccactgcc gcgcctgcgg cttgtccaac accgtgccga
      361 ccgggaaaag atttcccgtc cgtcccggcg ttatcaggag gccggattgg cagacaaacg
      421 cagcaaaatg ctcaccatgt gggtgactga ggacgagcac cggcgtctgc tggagcgctg
      481 cgagggtaag caactcgcgg cctggatgcg gcagacgtgc ctggacgaga agcccgcacg
      541 cgccggcaaa cttccgtcga tctcgccggc gctgcttcgt cagcttgccg gcatggggaa
      601 caaccttaac cagattgccc gccaggttaa cgccggtggt ggaagcggac acgaccgcgt
      661 gcagattgtc gccgcgctga tggccatcga tgccggactc gagcggttgc ggcatgccgt
      721 actggaaaag ggtgctgatg atgatcgtta agtttcatcc tcgggggcgc ggcggcggcg
      781 gcgggccggt cgattatctg ctgggtaaag atcgccagcg cgacggcgcc agcgttctgc
      841 aggggaagcc ggatgaagtc cgggagctta tcgacgcctc gccctacgcc aaaaagtaca
      901 cttccggcgt tctgtctttt gccgaacagg atttaccgcc cggccagcgc gaaaagctga
      961 tggcgagttt cgagcgggtt ctgatgcccg gactcgataa agaccagtac agcgtgctgt
     1021 gggttgagca ccgggacaag gggcggctgg agctgaactt cctgatcccg aacacggagc
     1081 ttctgaccgg caagcggcta cagccgtatt acgaccgcgc cgaccgtccg cgcatcgatg
     1141 cctggcagac catagtgaac ggcaggctgg ggctgcatga cccgaacgcg ccggagaacc
     1201 ggcgggtgct ggtctcgcct tccgcgctgc cggaagcgaa gcaggaagcc gcccaggcga
     1261 ttacgagcgg cctgcttgcc cttgcctcat ccggggagct gaaaacgcgt caggacgtca
     1321 ctgaggcgct ggaaagcgca ggttttgagg tggtgcgcac cacaaaaagc agcatcagca
     1381 ttgccgaccc ggacgggggg cgaaacatcc gactgaaggg agccatctat gaacagtctt
     1441 ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagac taccggcgag
     1501 atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact gagcgaaagc
     1561 gagaagagaa tcagcgccgc catcctcgac cacgaccgga agctgtcctc agccatgagc
     1621 cagcgcacga aagggatgct gcgcatggtc agccagacgt ggctgaccat cgttctggtc
     1681 tccgcgctgc tgatagcgtc gagcgcgggc attctgtggt ggcaggggca gcagatactc
     1741 gagaactata cgaccatccg ggagcagaag agcacgcagg ccatgctgtc agagaggaac
     1801 agcggcgtac agctctcgac ctgcggcgag cagagacgcc gctgcgtgag ggtgaacccg
     1861 gaagcgggac agttcggaga ggactcgagc tggatgatac tggcggggaa atagcacatg
     1921 acggagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa
     1981 aggctggacg agtgggagaa cgccttcgcg gaatggcgga cgatgtctgg tcttattcaa
     2041 cgggagaacg cggcgctgaa cgagcgcgtc acggtcttga gcaggcaggt gcagagtttg
     2101 agcgagcagc tgcgccggtt gtcgaaaggc tgaacgcgat agaaactcac cggcagcagg
     2161 agcgggcggt gcagcaccag aaagcgctgg agctggaacg atcgcagtgg cagcatcatg
     2221 gtccttcgct atgataagcc gggttctatt aattagttgt tatgtaaaga tatttaaatt
     2281 tttcaacatg aggtattttt atgaatacat atattttcct gggaggggct attgtggctg
     2341 aagtcatagg caccatcttt atgaaatatt cagaaggatt tacacgcctg tggccgtctg
     2401 taggaactgc aataagttat tgcgctgcct tttatctgct ttcccaaaca ttggaaaata
     2461 tacctaccgg tattgcttac gcaatttggt ctggtgcggg aattgtacta ataagcctag
     2521 ctggttggtt ggttagcggt caaaaacttg attggcccgc aattgcagga atgttcctta
     2581 tttgcgttgg agttttggtt attaatattt tttcaaaatc ttctgcgcat taacttttta
     2641 ccgaagcata ttggccaaag caaaaggatt tccttgagat cctttctttc tgcgcgtaat
     2701 ctttttccct gtaaacaaaa aaaccaccgc taccaacggt ggttcgtttg ccggaattca
     2761 gagctaccaa ctctttgttc cgatggtaac tggctttcac ggagcgcaga tacctaaact
     2821 gtcctttcag tgtagccgca ctcaggcttt cacttcaaga actctgtagg cattctctac
     2881 aatctctgct ccgtgaaccc agttaccaat ggctaccgcc actggcgatt tgtcgtgtct
     2941 ttccgggttg gactcaagtt gatagttacc ggaacgggcg tggtagtcgg actgaacggg
     3001 gggttcgtgc atacagtcca tcctggagcg aactgcctac ccggaaccga gtttcaggcg
     3061 tggaatgaga aaccgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaat
     3121 gcgggggagc acgagggagc caccaggggg aaacgcctgg tatctttaag ccgcatcggg
     3181 tttcgccacc gctgatttga gcgtcatttt cgtgatactc gtc
//
