LOCUS       CP052341                3851 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D17KP0018 plasmid pD17KP0018-4,
            complete sequence.
ACCESSION   CP052341
VERSION     CP052341.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609916
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:48:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,860
            CDSs (total)                      :: 5,736
            Genes (coding)                    :: 5,599
            CDSs (with protein)               :: 5,599
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 63 of 137
            Pseudo Genes (incomplete)         :: 64 of 137
            Pseudo Genes (internal stop)      :: 30 of 137
            Pseudo Genes (multiple problems)  :: 19 of 137
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D17KP0018"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD17KP0018-4"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(3566..3851,1..56)
                     /locus_tag="HJX28_29235"
     CDS             join(3566..3851,1..56)
                     /locus_tag="HJX28_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJK26124.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            join(3846..3851,1..753)
                     /locus_tag="HJX28_29240"
                     /pseudo=""
     CDS             join(3846..3851,1..753)
                     /locus_tag="HJX28_29240"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            734..1231
                     /locus_tag="HJX28_29245"
     CDS             734..1231
                     /locus_tag="HJX28_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJK26121.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRKVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            1238..1453
                     /gene="mbeD"
                     /locus_tag="HJX28_29250"
     CDS             1238..1453
                     /gene="mbeD"
                     /locus_tag="HJX28_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJK26122.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mbeD"
     gene            complement(1878..2105)
                     /locus_tag="HJX28_29255"
     CDS             complement(1878..2105)
                     /locus_tag="HJX28_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QJK26123.1"
                     /translation="MTKVIYSALAAIIALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DAGLIGYTDANGVKQQINKQEVKEVSEIPN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(2119..2271)
                     /locus_tag="HJX28_29260"
     CDS             complement(2119..2271)
                     /locus_tag="HJX28_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QJK26125.1"
                     /translation="MKTTDGRTIVADGKPQIDSDTGLVSYKDAQTGKTEHINRDKITNM
                     SELDN"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          3299..3483
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.701"
                     /coverage="86.0"
                     /evalue="5.789999999999997e-54"
     Typing          complement(2835..3316)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.741"
                     /coverage="92.0"
                     /evalue="0.0"
     Typing          47..1569
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.213"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3588..3850
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.135"
                     /coverage="83.0"
                     /evalue="7.68e-48"
ORIGIN
        1 gtctggaacg gttgcggcat gccgtactgg aaaaagggcc ggatgatgat tgttaaattc
       61 catccacggg gtcgcggcgg gggcgccggt ccggtggact atcttctggg taaagaccgg
      121 cagcgtgaag gtgccagcgt cctgcagggg aagccggaag aaatccggga gctgattgat
      181 gcgtcgccgt atgtgaaaaa atatacctcc ggggtgctgt cattcgcgga ggcggatctg
      241 ccgccgggtc agcgtgaaaa actgatggcc agctttgagc gggttctgat gcccggactc
      301 gataaagatc agtacagcat tctgtgggtg gaacacgcag acaagggtcg tcttgagctg
      361 aattttctga tccccaatac cgaattactg acggggaaac gcctccagcc gtattacgat
      421 cgtgccgacc ggccgcgcat cgatgcgtgg cagacagtgg tcaacggcag gctggggcta
      481 catgatccga acgacccgga gaaccggcgg gcgctggtca cgccgtcagg actgccagaa
      541 acgaagcagg aagccgcaga gacgattaca cggggtttgc tgatgttagc ctcatcagga
      601 gagctgaaaa cgcgtgatga cgtcacaggg gcgctcacag cggccggttt tgaggtggtc
      661 agaaccacaa aaaacagtat cagcattgcc gacccggacg gggggcgaaa tatccgactg
      721 aggggagcca tttatgagca gtcttttaac ggcctcgagg gacttcgagg agaaatcgaa
      781 agcacagcag cagcttaccg aagagaggct gaaagccgca ttcagcaagc acgagaacgt
      841 tgtcaaatcg gaactgaacg caagcgcgaa gagaatcaac gacgccatca gcgcccacga
      901 acaggggatg aacgcggcga tgcagtcgaa ccggctgagc gtgctgcgaa tggtcgggcg
      961 gacgtggctg accatcacca tggtgtcagg gctgctgttc gccagcctga gcggggtgct
     1021 gtggtaccag gggaaccgga tagcggcgaa cctggcggaa atcaggcagc agagagatac
     1081 gctgtcgaaa ctgcagatgc agacgtgggg cgtgacctat ctggaaaacc gcaacggacg
     1141 gtttctggtg ctgccggagg ggatgaaagc cgaaacgggc cggaaggtgg acaacaagac
     1201 gcggaacgcg gtgaagctgg tacgggagta gagcagaatg acggaactgg aaaaacaatt
     1261 gctgagcgcg ttagagcagc tacagcagga ctactcgaga aggctggacg agtgggagag
     1321 cgccttcgag gaatggcgga agatgtctgg tcttatgcaa cgggagaacg cggcgctgag
     1381 cgagcgcgtg tcgggcttga gcaggcaggt gcagagtttg agcgagcagc tgcgccggtt
     1441 atcgacaggc tgaatgagat tgagcaacag cgatacgggc aaacaacgga aaaggaacca
     1501 gagaagacag cagatctggt accggaacgg caacgcacat tatctcatgg tgacagggag
     1561 tatggagact gataaacgcc cgtctcaggt ggcctgagcg tgacggggat catggccatt
     1621 ttctgcgatt tcagcacaca gagagtcttt tttaaccgaa atcaccggct atgcagtgat
     1681 gtgaataagt gatgcatatg cgtaaaataa atcaggtgcg tttctggtgg ttttcggtgt
     1741 acttgccacc accttttgcc cgtcagccat cagaaacgcc ctgagtgcct ccggcgcggt
     1801 gcgagtaagg gatgttatgt ttaattggac ggttactagc atgtaaaaaa aaaccgccac
     1861 taggcggttt tttatgatca gttaggaatc tctgagactt ctttgacttc ctgtttatta
     1921 atttgctgct tcacgccgtt agcatcggta tagccaatca acccagcatc cgattctttg
     1981 ggtttgccat cgctcacaat ggtacggcca tcattcgtat gaattgcata gcttgtcctt
     2041 gtgcatcctg tcagagctga aagtgcaatg atcgcagcta gcgctgaata aattactttc
     2101 gtcatagaac gttccttatc agttatctag ttcactcatg ttggtaattt tgtcgcggtt
     2161 gatatgttct gtttttcctg tctgtgcatc cttgtaagaa accagcccgg tgtcactatc
     2221 aatctgaggc ttaccgtctg cgacgatagt ccgtccgtcg gtagttttaa tggcctgatt
     2281 ttaggaggtg ccggttactg agaaggttaa aactgaagtt attaagagaa accaatgctt
     2341 ttttagatat cccatatccc tagtactggc gaagttagta tctaacttac taataatagg
     2401 cattgttttg gcttctgcat cggaagggga gataaaatgt aaaaaaagaa atcttatgtt
     2461 aaatcaggtg gcagaatgct gtgttaaacg gctagttgct aatcataatc attttgtaac
     2521 gcagggggat ttcttccgcc gataaatgca ttccagtgat ggattcatta caaggaacat
     2581 cataaatccc atggatatcg atcctgcgaa gaaaggatct tcttgagatc atttttttct
     2641 gcgcgtaatc tcttgctctg aaaacgaaaa aaccaccttc cggtggcttt ttcgatggat
     2701 agaggatcct ggcagattct ctaaccgagg taactgggtg tacaagaccg caattaccaa
     2761 aactgttctt tcagtgtagc cgcagtcagg catccacttc aagacctctt taaataccat
     2821 cttgtacacc cttgttacca gtggctgctg ccagtggcgt tttttcgtgt ccgtccgggt
     2881 tggaatcaag atgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt
     2941 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     3001 gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     3061 cgcacgaggg agctaccagg gggaaacgcc tgggatcttt atagtcctgt cgggtttcgc
     3121 cgccactgat ttgagcgtca gattttgtga tgcttgtcag gggggcggag cctatggaaa
     3181 aacggcaacg ccgcatcctt tctccagccc ttcctgattt cagcgcagtc tctctatctt
     3241 gtcattcgtg agcaggtaac gcccgccgcc gtctcacggc aggagcgcag cgaccgagtg
     3301 agcgaggagg cgtcatgtca tcaccacctg cattttgcac ttacgccccg cagctgaatt
     3361 tttcccctgc cacatgaagc actttcccga attaccactt cgatttatca tattaagcca
     3421 gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgctaaaa
     3481 accactgacg cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc tttcccgtcc
     3541 gccacggcgt tatcaggagg cgattctggc agataaacgc aacaagatgc tcacgatgtg
     3601 ggtgacggaa gacgaacatc ggcggttgct tgagcggtgc gacggaaaac aactggcggc
     3661 gtggatgcgc cagacctgcc ttgatgagaa accgacacgc gccggcaggc ttccctcgat
     3721 ttcgccggcg ctgctgcgtc agcttgccgg catgggcaat aacctgaacc agatcgcccg
     3781 gaaagtgaac gccggtggtg ccggtcatga ccgggtgcag atcgtggcag cgctgatggc
     3841 gatcgatgcc g
//
