LOCUS       CP052335                3511 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D17KP0022 plasmid pD17KP0022-3,
            complete sequence.
ACCESSION   CP052335
VERSION     CP052335.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609917
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:44:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,490
            Genes (coding)                    :: 5,387
            CDSs (with protein)               :: 5,387
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 47 of 103
            Pseudo Genes (incomplete)         :: 50 of 103
            Pseudo Genes (internal stop)      :: 22 of 103
            Pseudo Genes (multiple problems)  :: 14 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D17KP0022"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD17KP0022-3"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(2235..3511,1..250)
                     /locus_tag="HJX29_28050"
     CDS             join(2235..3511,1..250)
                     /locus_tag="HJX29_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="QJK09926.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGL
                     PETKQEAAETITRGLLMLASSGELKTRDDVTGALTAAGFEVVRTTKNSISIADPDGGRN
                     IRLRGAIYEQSFNGLEGLRGEIESTAAAYRREAESRIQQARERCQIGTERKREENQRRH
                     QRPRTGDERGDAVEPAERAANGRADVADHHHGVRAAVRQPERGAVVPGEPDSGEPGGNQ
                     AAERYAVETAHADVGRDLSGEPQRTVSGAAGGDESRNGPDGGQQDAERGEAGTGVEQND
                     GTGKTIAERVRAATAGLLEKAGRVGERLRGMAEDVWSYATGERGAERARVGLEQAGAEF
                     ERAAAPVIDRLNEIEQQRYGQTTEKEPEKTADLVPERQRTLSHGDREYGD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            526..708
                     /locus_tag="HJX29_28055"
     CDS             526..708
                     /locus_tag="HJX29_28055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK09927.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(1308..1619)
                     /locus_tag="HJX29_28060"
     CDS             complement(1308..1619)
                     /locus_tag="HJX29_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK09928.1"
                     /translation="MRRAEMAYERGGDFLEDARKILNREAIGPRRSRFSIGSAPLTSIT
                     KSDAQISGGETRQDYKDTRRFPLAAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            1904..2245
                     /locus_tag="HJX29_28065"
     CDS             1904..2245
                     /locus_tag="HJX29_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJK09929.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          1646..1819
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1158..1679)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2236..3509
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccttcgagga atggcggaag atgtctggtc ttatgcaacg ggagaacgcg gcgctgagcg
       61 agcgcgtgtc gggcttgagc aggcaggtgc agagtttgag cgagcagctg cgccggttat
      121 cgacaggctg aatgagattg agcaacagcg atacgggcaa acaacggaaa aggaaccaga
      181 gaagacagca gatctggtac cggaacggca acgcacatta tctcatggtg acagggagta
      241 tggagactga taaacgcccg tctcaggtgg cctgagcgtg acggggatca tggccatttt
      301 ctgcgatttc agcacacaga gagtcttttt taaccgaaat caccggctat gcagtgatgt
      361 gaataagtga tgcatatgcg taaaataaat caggtgcgtt tctggtggtt ttcggtgtac
      421 ttgccaccac cttttgcccg tcagccatca gaaacgccct gagtgcctcc ggcgcggtgc
      481 gggtaagaga tgttatgttt aatgttgata acaggagtaa gagtgatgct tacatttatt
      541 ggtgtagtgg tagtcgccgt tattgcattt aaattattta tgcttttttt tagttcatcc
      601 gaatctgccg tcgaacgtgc tacccgtaaa tatatggaga accctacgag tgctaactat
      661 aaaataatgg tggctacacg catgagaaat gagaagaaaa gagggtgata tatgtttgag
      721 tttattggtt ttttattttt acttttggtg tgttatgtct ttatacaaat ggttgctgtc
      781 agggtgtttc ctgaatatgg agtacggaaa gcggaggaac gataccgtcg aaatccatca
      841 gcagaaaatg aggatctgtt atggagagct agaagtcgta taactaaacg caagtaatgt
      901 ttcctaagtg tgttgatagg caaacatggt gggagttact actctcggag ttaatgatct
      961 tcttgtgatc ctgtcgtttc agtcgtaatc tcttgctctg aaaacgaaaa aaccacctgg
     1021 ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccagg taactgggta
     1081 tacgagaccg cagataccaa aactgttctt tcagtgtagc cgcagtcagg cccatacttc
     1141 aagacctctg aatctctctc tcgtatatcc cagttaccag tggctgctgc cagtggcgct
     1201 ttgtcgtgct ttctcgggtt ggactcaaga tgatagttac cgggtaaggc gcagcagtcg
     1261 gactgaacgg ggggtttgtg catacagtcc agcttggagc gaactgccta cccggaactg
     1321 agtgtcaggc gtggaatgag ataaacgcgg ccataacagc ggaatgacac cggtaaaccg
     1381 aaaggcagga acaggagagc gcacgaggga gccgccaggg ggaaacgcct ggtatcttta
     1441 tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg
     1501 ggggcggagc ctatggaaaa acggcttcgc cgcggcccta tcgcttccct gttaagtatc
     1561 ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc tcgccgcagt
     1621 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct gtatcacata
     1681 ttctgctgac gcgccggtgc cgcctttttt ctcctgccac atgaagcact tcactgacat
     1741 cctcatccgt gccaacatag taagccagta tacactccgc tagcgctacg tgactggttc
     1801 agggctgcgc cccgaaaccc cactgacgcg cctgcggctt gtccaacccc gcgccgaccg
     1861 ggaaaggctt tcccgtccgt cacggcgtta tcaggaggcg attctggcag ataaacgcaa
     1921 caagatgctc acgatgtggg tgacggaaga cgaacaccgg cggttgcttg agcggtgcga
     1981 cggaaaacaa ctggcggcgt ggatgcgcca gacctgcctt gatgagaaac cgacacgcgc
     2041 cggcaggctt ccctcgattt cgcccgcgct gctgcgtcag cttgccggca tgggcaataa
     2101 cctgaaccag atcgcccgga aagtgaacgc cggtggtgcc ggtcatgacc gggtgcagat
     2161 cgtggcagcg ctgatggcga tcgatgccgg tctggaacgg ttgcggcatg ccgtactgga
     2221 aaaagggccg gacgatgatt gttaaattcc atccccgggg tcgcggcggg ggcgccggtc
     2281 ctgtggatta tcttctgggt aaagaccggc agcgtgaagg tgccagcgtc ctgcagggga
     2341 agccggaaga agtccgggag ctgattgatg cgtcgccgta tgtgaaaaaa tatacctccg
     2401 gggtgctgtc attcgcggag gcggacctgc cgccgggtca gcgtgaaaaa ctgatggcca
     2461 gctttgagcg ggttctgatg cccggactcg ataaagacca gtacagcatt ctgtgggtgg
     2521 aacacgcaga caagggtcgt cttgagctga attttctgat ccccaatacc gaattactga
     2581 cggggaaacg cctccagccg tattacgatc gtgccgaccg gccgcgcatc gatgcgtggc
     2641 agacagtggt caacggcagg ctggggctac atgatccgaa cgacccggag aaccggcggg
     2701 cgctggtcac gccttcagga ctgccagaaa cgaagcagga agccgcagag acgattacac
     2761 ggggtttgct gatgttagcc tcatcaggag agctgaaaac gcgtgatgac gtcacagggg
     2821 cgctcacagc ggccggtttt gaggtggtca gaaccacaaa aaacagtatc agcattgccg
     2881 acccggacgg ggggcgaaat atccgactga ggggagccat ttatgagcag tcttttaacg
     2941 gcctcgaggg acttcgagga gaaatcgaaa gcacagcagc agcttaccga agagaggctg
     3001 aaagccgcat tcagcaagca cgagaacgtt gtcaaatcgg aactgaacgc aagcgcgaag
     3061 agaatcaacg acgccatcag cgcccacgaa caggggatga acgcggcgat gcagtcgaac
     3121 cggctgagcg tgctgcgaat ggtcgggcgg acgtggctga ccatcaccat ggtgtcaggg
     3181 ctgctgttcg ccagcctgag cggggtgctg tggtaccagg ggaaccggat agcggcgaac
     3241 ctggcggaaa tcaggcagca gagagatacg ctgtcgaaac tgcacatgca gacgtggggc
     3301 gtgacctatc tggagaaccg caacggacgg tttctggtgc tgccggaggg gatgaaagcc
     3361 gaaacgggcc ggacggtgga caacaagacg cggaacgcgg tgaagctggt acgggagtag
     3421 agcagaatga cggaactgga aaaacaattg ctgagcgcgt tagagcagct acagcaggac
     3481 tactcgagaa ggctggacga gtgggagagc g
//
