LOCUS       CP052334                5010 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D17KP0022 plasmid pD17KP0022-2,
            complete sequence.
ACCESSION   CP052334
VERSION     CP052334.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609917
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:44:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,490
            Genes (coding)                    :: 5,387
            CDSs (with protein)               :: 5,387
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 47 of 103
            Pseudo Genes (incomplete)         :: 50 of 103
            Pseudo Genes (internal stop)      :: 22 of 103
            Pseudo Genes (multiple problems)  :: 14 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D17KP0022"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD17KP0022-2"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(4963..5010,1..552)
                     /locus_tag="HJX29_28015"
                     /pseudo=""
     CDS             join(4963..5010,1..552)
                     /locus_tag="HJX29_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655066.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /name="TIR domain-containing protein"
     gene            complement(604..1311)
                     /locus_tag="HJX29_28020"
     CDS             complement(604..1311)
                     /locus_tag="HJX29_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK09920.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(1308..2213)
                     /locus_tag="HJX29_28025"
     CDS             complement(1308..2213)
                     /locus_tag="HJX29_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="QJK09921.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replicase"
     gene            complement(2461..2736)
                     /locus_tag="HJX29_28030"
     CDS             complement(2461..2736)
                     /locus_tag="HJX29_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="QJK09922.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(2717..2962)
                     /locus_tag="HJX29_28035"
     CDS             complement(2717..2962)
                     /locus_tag="HJX29_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="QJK09925.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            3199..3360
                     /locus_tag="HJX29_28040"
     CDS             3199..3360
                     /locus_tag="HJX29_28040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK09923.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            complement(4160..4561)
                     /locus_tag="HJX29_28045"
     CDS             complement(4160..4561)
                     /locus_tag="HJX29_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJK09924.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 tactacaaaa acgcacttga aaaaatgaat gaggaacatg aaatattcgt gaatcgctca
       61 gtatcattag gtgatataga tgaagatgaa gccccacaaa aaataagaga gataataaga
      121 gatgaatatc ttagagatac atcagttctt atccttcttg ttggaactga aacaaaaaac
      181 agaaaacacg tagattggga actttactct agcatgagag atagtaccat taacaaaaaa
      241 tcaggaattt tagtcgtcaa tctcccgtca acaaatacta cgtacattcg ttcaacacat
      301 ggaactaatg aaaaatcaga agttcatcct accgtaactg aatggttttc tattgataag
      361 cgctcaactt atgaggaaag gttcccatat atgccagcaa gaataataga caacttcatg
      421 aagagcgggt cttatatatc agtggtcaac tggaatcaaa tagaaaacaa cccagaaaca
      481 ctcagaaaat taatagacat gacttataac gatagagaaa aagcaacgta tgatatgtcg
      541 agagaaatga gaatgcataa ttcatagtac tatccaagga gtagccttgg ctactccttt
      601 aatttaccag ttccacttat ctgtattgat tccaaactta ggcttcacat cattaacaaa
      661 gaaccggtta acatctttaa aaccttttaa aatacgctca taatcataat actgcatttg
      721 tattttattt atgctggttg catgaaatct catgacatca ccgctaaccc tatcaacaac
      781 ctcatctttg tctgatatgt acttatacca acgcttgtag ttgttctggt agttagttag
      841 aaatctattt aacaccatga gcatctcatt gctgaaatca taaatctcac gatctttttt
      901 agggtttagt attttcattt catcacgaat aaattggtaa gtctgataat atgatgaaat
      961 aacctcatca aaggcatcaa cgttttttct taatttgaaa attgctcttt ttgaatctag
     1021 gtgatttttt acactctttt tgtaaagtgt agcactctca ttgaaaagta tattcactcc
     1081 accaaggcta agtttctcaa ccttaaccga atacttttta agtaagtaga gggataccat
     1141 cagccaaata aaaacagata tcaaaagaat agaaagggcg ccagttacaa gaggtctatt
     1201 cttactaaaa actgtgtaaa cagacggaat gtaattagat atatcaacag tacacatcac
     1261 ggcaataaat gctaaaaaaa ctatagtcca aagagttacg aaggttttca tcttatttca
     1321 ccatttttct tgatataaaa atacttccgc ctgcttattc ccatagctac ccaaggtttt
     1381 atttcctcta ggcttccttt cctccttgac tgtcctgact ttttccctct ctttgattga
     1441 atttcagagg aatgagtaat atcaacaaat ttactgaatt cttcaggtga aaatttattt
     1501 gatgtccatt tagcaatgct cttagctatc cctcttacct cattttcatc cagaggccgt
     1561 gcaaattgca ggttgtatgc gcaagcgcgt tcgtagcagg cttgcagcca ctgctcatac
     1621 tcaggccagc cctgacggat ggcgcgatat gcccacttac gggttttgtc gaacaacgtg
     1681 cagttacggc ctaagccgta gtcggcaacg atttccttgt cattccctgt gttcagatcg
     1741 agaaagtcgg ccagccagtc cagagtgtag agcttcggct gccacacggc gattttccag
     1801 tggttgtggt tcggattttt gcagatgagc cccgaatatc cagcgtcagc ctctaatttt
     1861 ttacgtagcg cattctccac ggaggcggcg tagcgtaacg gttttatttt gccgtccggc
     1921 gcggtgcgta tcgagttttc cagcgcgtac agcaggtgcg cgtgtccgtt ttcggggtta
     1981 gttatggtca gcgtcggtgc aggcgcgttg cggtcgctcc agtcgatgga ggaaccggcg
     2041 cggtcaacgt caaatcccag ccagaacatg gcgtgaggct gattgaactg gatatatttc
     2101 gccagaatag cgcgttcctt accggcgata cggacaccaa agtgcagatc gtccgaaaag
     2161 tacggcttgt gtggtaagcg ttcgttgaaa agttcgagtg cggcgttatg caatgtgccc
     2221 tgcgcttatg gcgggctgtt gtctggcacg gttcatgtgc taatatcctt tcaaggtttc
     2281 gacctagccc tggttatcat gcgatccgcc aagatatgac atgatttccg gggctttgtt
     2341 ttttctgagg ctaacgttac accgactttg cctttgtgtc gatctgacag cgacttaccc
     2401 taacatccgt tgtacgcctt gcatgaaaag cccgcatcaa gcgggctata tcgacttttg
     2461 ctatttgtca ccatagtgat aacgggctgc gtagatgatg atttctccgt tctggattga
     2521 atagaccaga cggtggctct gatcaatcct gcgggagtag agtgccggat ctttgtggtg
     2581 ccgcagccgt tcgggttttc ctatacctgt cagcggatca gcctcgatgc tgagcaacag
     2641 ttgccggata cgctcaaact tctttttgtc agtatcacgc cagtagttaa ggtcatcagc
     2701 gccattccta gtgaacttaa tattccactt cgacaaattc gccgcgctcc gcctgtttca
     2761 ttgactcatt gatatgctct gcgttcgccg gattgctgaa cagatagacc gtttcgatta
     2821 gcgcctcata ctctcccgca tcgatcataa caatatcagg cgcatcacgg cgcatgacac
     2881 gtaccggttc agcatcatca gtgacgcgct tcatcgtctc ggcaaaatgt ttacgggcat
     2941 cagtaaaatt gattgcatgc atgttgttaa cccccatttg ttggggctta gcatagaaca
     3001 tgtacgcaaa taagtacata aagatatttc cctaaaaacc cacaaccaaa tcagctcaaa
     3061 atgtgaactc aatcgaataa ccacaaaaac aacaagtcaa tacattgaag cataatttca
     3121 agcaattaca tttatgaagt gcggcacaat ttcgtaccgc taggagctta gctcctaaaa
     3181 cgagcaaagg aaaagtaaat ggcaaaacat tggtctgggc aagaatgtcc aaaaacaggt
     3241 acttacggtc aatatcatga taccgacaac tcatacgctg gttctgaata tgatcgccat
     3301 gtgagcaagg gtgataagtt ccctccgagc aaaaacaacc accacttcca agaaaaataa
     3361 aatatcttca gaagtctaaa ctaaaagccc tctggggctt tttttgtacc tgccattcac
     3421 ttgatttacc ataacatccg ttatcagcac cgcctgagat gccctcaggg cgtttctgac
     3481 gggaaaccgg taaacaaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct
     3541 gtttttacgg catgcatgag tatgcgcaca agtgcatagc gcggaaattg acaccattca
     3601 gccaggggaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa
     3661 tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt cagcacgttc
     3721 aggaatcggt tcgtccacgg ccggcaatca cgttatggca agtcgccgga cgactcacca
     3781 cacgattccc cggcgactct tcactagagt cgtacccaga gtcgccacgt ctaaaaaaca
     3841 accaaacggg gagtcggatc tagagtcggg tcgttattaa ctcattgttt ttataggcct
     3901 gagtcgttac cgactctatg acgactcgcc ggggactcta tgacgactcg tcatagagtc
     3961 ggatctatat aaataaaatt taaaaaacga aatttcagta aagtaagctg ctgatattaa
     4021 atacaaatct gcaaaattaa aattttgcgt ggggtgtggg tatttttcgt ggtgaggagc
     4081 gttcagagat ctgacgattt tcgggaaggt tcaggaactc cccggaatgg ccggagagga
     4141 aaactgaagc atgacggggt caggattttt tccagaattt ccaccacggc gccgggggtt
     4201 cagttaattt tgcctggtga ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt
     4261 gcgaatcctt cgataaaatc aatatgttaa ggtgttcaat ctgctgtttc agttgacgaa
     4321 tgacatgttc actctggtca gcaggcgctg tatcacgctg ttcaggtact tgtcgagtaa
     4381 ggtgttcatt cttcggttgg tcaaacggtc cgaaaacacg taaaagctct gatatatcga
     4441 ccatttttgt atttcgttca ccgctacaag tggacagttc acctgttttg attaacctgt
     4501 gcagcgttgt ccggctctta cccgtcagcc gtgcagcctc actaatagac actctggtca
     4561 tcagttatca tcctttaata cattcagtct caaggaaaca ggggccagct tacgacatac
     4621 tggtccagaa gtgatgtgtt tttcagaagg gcgatgaaac acaggagtga ccgtgcaaaa
     4681 tgcaggcaac aaatgacttt ccgcttcctc gcgcattcgc tcactacgtt cggtcataag
     4741 actgcggcga gcggtacgta agaataaagt gaggtgatag ctctcttctc aaggcgttta
     4801 gggaagcaac gagcccgccc taaaccagtc atggcgagac cttgctgaag ggaccctatg
     4861 tgtgctggct gagaaaaaaa gagctctcta aaagagagct gttatctgat ataggttcaa
     4921 gtgcaaaata gagtgcacac aaacaaaaaa aggacaataa aaatgggaaa gcataaggta
     4981 ttcattagct accaccatgc gaatgatcag
//
