LOCUS       CP052331                3223 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain D17KP0032 plasmid pD17KP0032-3,
            complete sequence.
ACCESSION   CP052331
VERSION     CP052331.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609918
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:46:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,612
            CDSs (total)                      :: 5,489
            Genes (coding)                    :: 5,362
            CDSs (with protein)               :: 5,362
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 62 of 127
            Pseudo Genes (incomplete)         :: 63 of 127
            Pseudo Genes (internal stop)      :: 24 of 127
            Pseudo Genes (multiple problems)  :: 22 of 127
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D17KP0032"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pD17KP0032-3"
                     /geo_loc_name="South Korea: Chungbuk"
                     /lat_lon="36.80 N 127.70 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            264..608
                     /locus_tag="HJX30_28040"
     CDS             264..608
                     /locus_tag="HJX30_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJJ09220.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            547..1311
                     /locus_tag="HJX30_28045"
                     /pseudo=""
     CDS             547..1311
                     /locus_tag="HJX30_28045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1286..1771
                     /locus_tag="HJX30_28050"
     CDS             1286..1771
                     /locus_tag="HJX30_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJJ09221.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="mobilization protein"
     gene            1775..1990
                     /gene="mbeD"
                     /locus_tag="HJX30_28055"
     CDS             1775..1990
                     /gene="mbeD"
                     /locus_tag="HJX30_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJJ09222.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="mbeD"
     gene            2158..2490
                     /locus_tag="HJX30_28060"
     CDS             2158..2490
                     /locus_tag="HJX30_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="QJJ09223.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             2159..2490
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          2..189
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.147"
                     /coverage="87.0"
                     /evalue="5.66e-69"
     Typing          complement(2741..3128)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.265"
                     /coverage="90.0"
                     /evalue="3.9799999999999997e-128"
     Typing          599..2088
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgaggaggc gtcatttcat caccacctgc attttgcact tacgcccggc agccgcattt
       61 ttcccctgtc acatgaagca ctttcataaa ttgccactcc gtcataacat gtttagccag
      121 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac ccgctaaaac
      181 cactgccgcg cctgcggctt gtccaacacc gtgccgaccg ggaaaagatt tcccgtccgt
      241 cccggcgtta tcaggaggcc ggattggcag acaaacgcag caaaatgctc accatgtggg
      301 tgactgagga cgagcaccgg cgtctgctgg agcgctgcga gggtaagcaa ctcgcggcct
      361 ggatgcggca gacgtgcctg gacgagaagc ccgcacgcgc cggcaaactt ccgtcgatct
      421 cgccggcgct gcttcgtcag cttgccggca tggggaacaa ccttaaccag attgcccgcc
      481 aggttaacgc cggtggtgga agcggacacg accgcgtgca gattgtcgcc gcgctgatgg
      541 ccatcgatgc cggactcgag cggttgcggc atgccgtact ggaaaagggt gctgatgatg
      601 atcgttaagt ttcatcctcg ggggcgcggc ggcggcggcg ggccggtcga ttatctgctg
      661 ggtaaagatc gccagcgcga cggcgccagc gttctgcagg ggaagccgga tgaagtccgg
      721 gagcttatcg acgcctcgcc ctacgccaaa aagtacactt ccggcgttct gtcttttgcc
      781 gaacaggatt taccgcccgg ccagcgcgaa aagctgatgg cgagtttcga gcgggttctg
      841 atgcccggac tcgataaaga ccagtacagc gtgctgtggg ttgagcaccg ggacaagggg
      901 cggctggagc tgaacttcct gatcccgaac acggagcttc tgaccggcaa gcggctacag
      961 ccgtattacg accgcgccga ccgtccgcgc atcgatgcct ggcagaccat agtgaacggc
     1021 aggctggggc tgcatgaccc gaacgcgccg gagaaccggc gggtgctggt ctcgccttcc
     1081 gcgctgccgg aagcgaagca ggaagccgcc caggcgatta cgagcggcct gcttgccctt
     1141 gcctcatccg gggagctgaa aacgcgtcag gacgtcactg aggcgctgga aagcgcaggt
     1201 tttgaggtgg tgcgcaccac aaaaagcagc atcagcattg ccgacccgga cggggggcga
     1261 aacatccgac tgaagggagc catctatgaa cagtctttta acgctggcga aggacttaga
     1321 gcagaaatcg aaagcgcagc agcagactac cggcgagatg ctgaaagccg cattcagcga
     1381 gcacgagaag tctgtcagag cggaactgag cgaaagcgag aagagaatca gcgccgccat
     1441 cctcgaccac gaccggaagc tgtcctcagc catgagccag cgcacgaaag ggatgctgcg
     1501 catggtcagc cagacgtggc tgaccatcgt tctggtctcc gcgctgctga tagcgtcgag
     1561 cgcgggcatt ctgtggtggc aggggcagca gatactcgag aactatacga ccatccggga
     1621 gcagaagagc acgcaggcca tgctgtcaga gaggaacagc ggcgtacagc tctcgacctg
     1681 cggcgagcag agacgccgct gcgtgagggt gaacccggaa gcgggacagt tcggagagga
     1741 ctcgagctgg atgatactgg cggggaaata gcacatgacg gagctggaaa aacagttgct
     1801 gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt gggagaacgc
     1861 cttcgcggaa tggcggacga tgtctggtct tattcaacgg gagaacgcgg cgctgaacga
     1921 gcgcgtcacg gtcttgagca ggcaggtgca gagtttgagc gagcagctgc gccggttgtc
     1981 gaaaggctga acgcgataga aactcaccgg cagcaggagc gggcggtgca gcaccagaaa
     2041 gcgctggagc tggaacgatc gcagtggcag catcatggtc cttcgctatg ataagccggg
     2101 ttctattaat tagttgttat gtaaagatat ttaaattttt caacatgagg tatttttatg
     2161 aatacatata ttttcctggg aggggctatt gtggctgaag tcataggcac catctttatg
     2221 aaatattcag aaggatttac acgcctgtgg ccgtctgtag gaactgcaat aagttattgc
     2281 gctgcctttt atctgctttc ccaaacattg gaaaatatac ctaccggtat tgcttacgca
     2341 atttggtctg gtgcgggaat tgtactaata agcctagctg gttggttggt tagcggtcaa
     2401 aaacttgatt ggcccgcaat tgcaggaatg ttccttattt gcgttggagt tttggttatt
     2461 aatatttttt caaaatcttc tgcgcattaa ctttttaccg aagcatattg gccaaagcaa
     2521 aaggatttcc ttgagatcct ttctttctgc gcgtaatctt tttccctgta aacaaaaaaa
     2581 ccaccgctac caacggtggt tcgtttgccg gaattcagag ctaccaactc tttgttccga
     2641 tggtaactgg ctttcacgga gcgcagatac ctaaactgtc ctttcagtgt agccgcactc
     2701 aggctttcac ttcaagaact ctgtaggcat tctctacaat ctctgctccg tgaacccagt
     2761 taccaatggc taccgccact ggcgatttgt cgtgtctttc cgggttggac tcaagttgat
     2821 agttaccgga acgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccatcc
     2881 tggagcgaac tgcctacccg gaaccgagtt tcaggcgtgg aatgagaaac cgcggccata
     2941 acagcggaat gacaccggta aaccgaaagg caggaatgcg ggggagcacg agggagccac
     3001 cagggggaaa cgcctggtat ctttaagccg catcgggttt cgccaccgct gatttgagcg
     3061 tcattttcgt gatactcgtc aggggggcgg agcctatgga aaaacggcaa ctccgcatcc
     3121 ttttcttggc ctttcctgct tcgagcggca tttgcttatc ccgtaatctg tgagctggta
     3181 acgcccgccg cagtctcacg gcaggagcgt agcgaccgag tga
//
