LOCUS       CP052322                5219 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0035 plasmid pE16KP0035-2,
            complete sequence.
ACCESSION   CP052322
VERSION     CP052322.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609921
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:41:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,506
            CDSs (total)                      :: 5,381
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 50 of 110
            Pseudo Genes (incomplete)         :: 54 of 110
            Pseudo Genes (internal stop)      :: 24 of 110
            Pseudo Genes (multiple problems)  :: 18 of 110
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0035"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0035-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(5062..5219,1..373))
                     /locus_tag="HJX33_27460"
     CDS             complement(join(5062..5219,1..373))
                     /locus_tag="HJX33_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJJ83598.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTAWTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="mobilization protein"
     gene            complement(351..893)
                     /locus_tag="HJX33_27465"
                     /pseudo=""
     CDS             complement(351..893)
                     /locus_tag="HJX33_27465"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1051..1374)
                     /locus_tag="HJX33_27470"
     CDS             complement(1051..1374)
                     /locus_tag="HJX33_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJJ83590.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1439..1630
                     /locus_tag="HJX33_27475"
     CDS             1439..1630
                     /locus_tag="HJX33_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ83591.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1716..1895)
                     /locus_tag="HJX33_27480"
     CDS             complement(1716..1895)
                     /locus_tag="HJX33_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ83592.1"
                     /translation="MSEEAEKRLDVHFLLTHLCGISHPVWRTFHTIRTDAAWLSQYTLR
                     YRYVTGSGLRPDTR"
                     /name="hypothetical protein"
     gene            2817..3206
                     /locus_tag="HJX33_27485"
     CDS             2817..3206
                     /locus_tag="HJX33_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ83593.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(3270..3554)
                     /locus_tag="HJX33_27490"
     CDS             complement(3270..3554)
                     /locus_tag="HJX33_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ83594.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="hypothetical protein"
     gene            complement(3572..3769)
                     /locus_tag="HJX33_27495"
     CDS             complement(3572..3769)
                     /locus_tag="HJX33_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ83595.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            3981..4277
                     /locus_tag="HJX33_27500"
     CDS             3981..4277
                     /locus_tag="HJX33_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ83596.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            complement(4822..5055)
                     /gene="mbeD"
                     /locus_tag="HJX33_27505"
     CDS             complement(4822..5055)
                     /gene="mbeD"
                     /locus_tag="HJX33_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJJ83597.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="mbeD"
     Typing          complement(1716..1802)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.47e-38"
     Typing          1814..2370
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.462"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10..1061)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.86"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(4719..5191)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="1.38e-78"
ORIGIN
        1 tctgcttcct gatttcttcc aggttcgcct gtatcatccc gccaagatac cagagcgtcc
       61 cgccactcac ggtaaacagg aaaatgacca tccccagtaa catcatgccc gtattccctg
      121 ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt
      181 ttttcagtga ttcctctgtc caagctgtga acagggctat agcgtctttg attttcgtct
      241 cgtttgatgt cacagccttg cttacagatt tttcgagctt cctgaactcg tcgttcagta
      301 ttgtctctgt agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgta
      361 aaagattgct cataaaatgc tcctttcagc ctgatattct tcccaccgtt cgggtctgca
      421 atgctgatac tgcttcgcgt caccctgacc actttaagcc ctgcctctgt gagcgcctga
      481 atcacatcct gacggccttt tatctccccg acatgataaa gagcgtctat cccgcgtgtg
      541 acgctctctg caagcgcctg tttcgtttcc ggcaggttat cagagagagt cagtatccgg
      601 cggttctccg gggcgttggg gtcatgcagc ccgtaatggt gattcaccag cgtctgccag
      661 gcatcaattc tcggcctgtc tgcccggtcg tagtacggct ggaggcgttt tccggtctgt
      721 agctccatgt tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc
      781 cacaggatgc tgtactgatt tttttcgaga ccgggcatca gtacacgctc aaagctcgcc
      841 atcacctttt cacgtcctcc cggtggcagc tccttctccg cgaacgacag cacaccggac
      901 gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc gtacttcttc cggattaccc
      961 tgaagcaccg ttgccccttc ccggttacgc tcccttccca gcaggtaatc aaccggacca
     1021 ctgccaccac cttttccccg ggcatgaaac ttaactatca tcccgcgtcc cctgttccct
     1081 gacggccaga cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg
     1141 aacccggtca ccggaagacc actgcccgct gttcaccttc ctcgctgtct gattcaggtt
     1201 attcccgatg gcggccagct gacgcagtaa cggcggtgcc agtgtcggca gcctcccgga
     1261 acgggcaacc ggctcaccca ggcagacccg ccgcatccat accgccagct gtttaccctc
     1321 acagcgttca agtaaccggg catgttcatc atcagtaacc cgtattgtga gcatcctctc
     1381 gcgtttcatc ggtatcatta tccccatgaa cagaaatccc ccttacacgg aggcatcagt
     1441 gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt ataagaagcc agacattaac
     1501 gctgctggag aagctcaacg aactggacgc ggatgaacag gcagatatct gtgaatcgct
     1561 tcacgaccac gccgatgagc tttaccgcag ttgcctcgct cgcttcgggg atgacggtga
     1621 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtgag cggatgccgg
     1681 gagctgacaa gcccgtcagg gcacgtcagc gggttttagc gggtgtcggg gcgcagccct
     1741 gacccagtca cgtagcgata gcggagtgta tactggctta accatgcggc atcagtgcgg
     1801 atggtatgaa aagtgcgcca taccgggtgt gaaatgccgc acagatgcgt aaggagaaaa
     1861 tgcacgtcca gccgcttttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     1921 ctgcggcgag cggtatctac tcactcaaaa acgaaatggt gttatccaca gaatcagggg
     1981 ataacaccgg aaaaaacatg tgagcaaaag accaggaaca ggaagaaggc cgcgccgctg
     2041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2101 aggtggcgaa acccgacagg acttaaagat accaggcgtt tccccccgga agctccctcg
     2161 tgcgctctcc tgttccgacc ttaccgttta ccggatacct gtccgccttt ctcccttcgg
     2221 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt aacgccactg gcagcagcca
     2401 ttggtaacta aaaagtggat ttagatacgc agaactcttg aagttgaagc cttatagcgg
     2461 ctacactgaa aggacatctt ttggtatctg tgctccactt aagccagcta cccggttagg
     2521 taatctgcca ggattagcta accttcgaaa aaccacctgc cagggtggtt ttttcgttta
     2581 caggcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttttct
     2641 ctaaactgca atcccatcag ttcaggagag aaagatagta taattgccct ctcttatatg
     2701 gaaccaccat tcaatcacat catggggata atataagtga aaacgttcgc attgtttatt
     2761 tttacgtgta tatgtattta tagattggaa ttttaacaaa ataaaatgga gttaatatgt
     2821 tcaaatcatc agtaaagtca ataacatcga gtactatact gaccttactt ataggatgtt
     2881 caagtactga aacaaacaaa aacattgttt acaaagttgg tggtgacagc atcattctga
     2941 ataaatattg catacttaaa atgtaccctt cccaatcaga aaaaaacaaa ggcgtcataa
     3001 tagaagtaaa cacagatagt ttatgtaaga aaaaactaaa cgaaataact agtaaaaata
     3061 tagggaaacc cctgatttca tattttgatg gaaaagtaat atcccaacct gcaatcattc
     3121 attctgcact aaatccaacc cattattatc aacctgtgaa cgatgaaaca acagtgatgg
     3181 aaatcataaa agcatattca aaataatatt aatagatata atcctgctta aggtatagct
     3241 taggcaggat tatatctatt cagctcttat tataaggtag tgtatacatg aataatctgc
     3301 cctgtcccac tgacactttt aaatttaatt ttatcaacac cagcgccctt gcaagagatc
     3361 tgataagatg ctggaccaat aacgttaact gttcttggcc cattaggggc ataccagata
     3421 aaagcaataa atggaccggg tacattagac aatgtatcaa aatagacagt gtacgcgata
     3481 tcaccagtat gattttcaag ttcaacccaa ggaccaaaaa agaaaggagg agcccaaaca
     3541 ttttgagaaa tcatataatc tcctgattag ttcaatgcga atgttcttcc cattcactct
     3601 cgcaagaaac atctataagc tcaacatcta gcccactaac agaatcagag aaatacttat
     3661 aaacatcatc ccttgttccc ataaggtctg ccgaacatga tagttcacaa ccattcttat
     3721 tcctaactac aaataagaaa cggtgaagca atactttttc ttctttcatt ttatccctcc
     3781 ctttatttac acagaatata ctgtatatat aaacactagt tttatataca gtataaatta
     3841 cgccgcatta tttttcctgt caatgatatg aattttcatt tttgtgatct gaatcaccct
     3901 tataaaaatc aggaagggaa gattcgcagc agaaaaacag caccggataa catcaggaga
     3961 aaaaacagag aggagataac gtgagcaaaa caaaatctgg tcgccaccga ctgagcaaaa
     4021 cagacaaacg cctgctggct gcacttatcg ctgccggata cgaagagcgg acagcccgtg
     4081 acctcatcca gaaacacgtt tacacactga cacaggccga cctgcgccat ctggtcagtg
     4141 aaatcagtaa cggtgtggga cagtcacagg cctacgatgc gatttaccag gcaagacgca
     4201 ttcgtctcgc ccgtaaattc ctgaacggaa aaaaaccgga agaagggctg aacgcctgga
     4261 agggtgattt accataatca cttattgtac gcaccgctga aacgcgttcg gcgcgatcac
     4321 ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa actgccgcga tcgcacccgg
     4381 taaattttaa ccacatgcat agctatgcag ctatgtgaat catgcaggaa ttgccagcca
     4441 gagacagctg aaacggattt ttttcatgct ttcggaagcg aagaagacgg ggaccggagc
     4501 gggaaaacag aagttcaggg gaatgagcgc gatctggcaa tagaggcaaa acacagcaac
     4561 aaaagacaca ccagaatcgc gcccgtatgc gttttaacgc gttcctgtgt tttcagcaag
     4621 gtctgactga agcgcgttcc cggtgccccc gaaccaacca ttatcccaaa aaatgcaccg
     4681 gaaacgcgct gacaacattt tagcctgtct ctgattacca tcccagcgaa gggccatcca
     4741 gtgtccgttc ctgtatttcc agctggcgct cacgctccag ggataacgca tgttcgcgtt
     4801 cctgtatcag ccgttcgtcc cttatctcct gttctcgctg tataactggc tcaagcgttc
     4861 tgtctgctcg ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat
     4921 cgcgttgtcc cgttgcgtaa gcgaaaacat tttctgcaat tccacgaagg cgctctccca
     4981 ttcgttcagc cgctgcatat agtcctgttg cagttgctct aatgcgttca gcaaatgtct
     5041 ttccagctcc gtcatgctct gttactcctg taccagcctt acggcattct gtttcccgtt
     5101 ttccatcgtc cagttcgttt cggctttcat cccctcgggc aacaccagaa aacgtccgtt
     5161 gctcccctcg tgataacgca cgccccatgt cttcgcgttc agcttctcca gattctcgt
//
