LOCUS       CP052318                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0093 plasmid pE16KP0093-3,
            complete sequence.
ACCESSION   CP052318
VERSION     CP052318.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609922
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:48:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,587
            CDSs (total)                      :: 5,460
            Genes (coding)                    :: 5,327
            CDSs (with protein)               :: 5,327
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 66 of 133
            Pseudo Genes (incomplete)         :: 64 of 133
            Pseudo Genes (internal stop)      :: 27 of 133
            Pseudo Genes (multiple problems)  :: 22 of 133
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0093"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0093-3"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(548..697)
                     /locus_tag="HJX34_27840"
     CDS             complement(548..697)
                     /locus_tag="HJX34_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJK20514.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(782..1039)
                     /locus_tag="HJX34_27845"
     CDS             complement(782..1039)
                     /locus_tag="HJX34_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK20515.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(1049..2734)
                     /locus_tag="HJX34_27850"
     CDS             complement(1049..2734)
                     /locus_tag="HJX34_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJK20516.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            3137..3556
                     /gene="exc2"
                     /locus_tag="HJX34_27855"
     CDS             3137..3556
                     /gene="exc2"
                     /locus_tag="HJX34_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJK20517.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            3862..4125
                     /locus_tag="HJX34_27860"
     CDS             3862..4125
                     /locus_tag="HJX34_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJK20518.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            4115..4414
                     /locus_tag="HJX34_27865"
     CDS             4115..4414
                     /locus_tag="HJX34_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJK20519.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4476..4853)
                     /locus_tag="HJX34_27870"
     CDS             complement(4476..4853)
                     /locus_tag="HJX34_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJK20520.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            4922..5101
                     /locus_tag="HJX34_27875"
     CDS             4922..5101
                     /locus_tag="HJX34_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJK20521.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5129..5659)
                     /locus_tag="HJX34_27880"
     CDS             complement(5129..5659)
                     /locus_tag="HJX34_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK20522.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5666..6397)
                     /locus_tag="HJX34_27885"
     CDS             complement(5666..6397)
                     /locus_tag="HJX34_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJK20523.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(6397..8361)
                     /locus_tag="HJX34_27890"
     CDS             complement(6397..8361)
                     /locus_tag="HJX34_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJK20524.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7734
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..445
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.085"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(5670..6397)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gcgaaacccg acaggactta aagatcatca ccgtttccga tgaatcgctc cctcttgcgc
       61 tctcctgttc caaccctgcc gtttcccgga tacctgttcc gcctttctcc cttgcgggaa
      121 gtgtggcgct ttctcatagc tcacactctg gtatctcggc tcggtgtagg tcgttcgctc
      181 caagctgggc tgtaagcaag aaccccccgt tcagcccgac tgctgcgcct tatccggtaa
      241 ctgttcactt gagtccaacc cggaaaagca cgcaaaacgc cactggcagc agccattggt
      301 aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc
      361 caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac
      421 cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt
      481 ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      541 atcttttcta ctgaaccgcg atccccgtca gttcagagga ggacgatggt gccaccgttc
      601 caccctgaac atcccggata tagtttgcct gacatgcgac cagaagaaag cctgaaaaaa
      661 tcaatataaa aagaaaaaca acctttacct ttttcatata ccttccctgt atttaaaaac
      721 cccggtattc atccctgaac accggaagtc tgtatttcaa atcatccttg ataacttttg
      781 cttaccagtt accatcccgg taaacaaagg atacaaagta atcaaattta ctaatatcga
      841 ttacattttt aaagtgtggc tgtaatatcg aaacccatga tttttcaaca tcaaaccaac
      901 cattattaat attatccttt aaaggcatcc ccagactttc aatgacagaa ccatcatcac
      961 cgaagtcttt tgagtattca ccacctttaa actcttcggt tttcttatca aaccaatgaa
     1021 tatgtaattt aagccccata tttacctctt aaagatattt tttaatgttt cgttttggat
     1081 ccggcccttt aacctgctta cccgtttttg gatcgaatgc cccgaggtgt tcgccatcac
     1141 tggcgcggta cccttcaagc tcaccgtgct gggaatccca ttcataaatt ttacgctttt
     1201 tatcaccata ccatcgggcc cgcttaccac caccaccttg tttaggggtt ttaggtttac
     1261 cctctttcaa ctctcccaac cccttaatat cttcagtctt gggatcagga aaataatcat
     1321 ggccgtaatc tttagttccc ttacgaggtt ttttcttttc ctcatttaat ttattttctg
     1381 catccttcgc ttttttctcc ttttgtttcc ggctctccat cgccccggaa agcgcagcct
     1441 tatccctgga taaggattca ttcatttcgt ttaaagatgt ctgggcagct tttaatttct
     1501 cattctgatt ctgaatatcc ttatcaagat agcgaacctg atcctgccac ccggagccat
     1561 attcatgagg cgggttataa gtcacaaaat catctttatt tgcctctgcc tcttttacct
     1621 ttttatcggc ttcctgaaca gcagcccttg caccggggat cgccttctct gtcgatgcaa
     1681 tgcgactgtt taaggtcgca atatttttat cttcagcatc cagctcagct ttcgctttat
     1741 catactctct tttcagcccc tcttccgggt gagcagcgtc ccatgcctgc tggcgacgct
     1801 tttcttcctc cagacgctgt tttacctgtg ccggagtaag aacatccgtc accgacacat
     1861 agaccggttt ctgtccgctc tctttcggga aacgaataac ggcctcacgg gagttaccac
     1921 cggccgtaaa accagccgga cgtgaatcac ctttttcagc aataatgcct tttggcgggg
     1981 ctttcgcggc cggaacacct ttaggtacgc tcacctgcag agacgggaga cccggaatcg
     2041 acacactgaa tacccccgga cgttttgtcg gtttcgcatc caccacagga acactcattg
     2101 gccggccggt gacaaccgca atatgctgcc gctcgtcctt cacaatatcc gccacacgtt
     2161 tagtgacgct gaccgtcgcc tggtccagcg gcagggagct gaccggcgtc tccgttaccg
     2221 tgtctgccgg caatgacgtc acgatttttg acatcatttt cggatcatct tttgctatct
     2281 cagaaggcag cactccgtaa atcgcaatcc cccacagacc aaacttaaac ggacctttca
     2341 gggcagccag cacatcagca acagcggccg acaacgcatc gccggaaacg gataaagcca
     2401 gtccttcagc ccccggagtg gccagggcag gcagaccgaa ggccatcgcg gtggccgaag
     2461 actgtccgcc accagagctc ccgctatgat tgccgctgtt accgccatta ccaccgggtt
     2521 tactcccgcc gttaccaaac tcccccgggt tatagttatg aatatccccg ttaggtgtat
     2581 gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc accacctgat gaagggccac
     2641 ctttacctga tgtaccgttc acattcccac tggcctgacc accattgtgt cccagacctg
     2701 aattacccgg acctcgtccg tctccgccac tcatatactt ttcctctttg acttttaaaa
     2761 caataaacta aaataaaata ctgtacatat aaccactggt tttatataca gtataaaaat
     2821 tacgctgcct tgtttttcat gtcaagtctg taaatttgcg tttttgtgaa aatgatcaaa
     2881 aaaacagcca ctcacaccat gcgcaatccc ctccagatgc aacaaaaacc agcacaaacg
     2941 gcgccgttct ccggcctcag tatgttttac tgaaatcatt atatgagata ccagaaagcc
     3001 ccctccggga caggaaaaga aacggggggt ctggttcggg ttgttcggtc actgaacacg
     3061 gtcagaaact gatgataaga tagcctgcgg atatataact caatgagaaa aaagaattaa
     3121 aggtaaggag acgctcatga gacgttccgg cagcaaactg tgtcttatcg ccatgataac
     3181 agcgacagca actctcactg cctgtgctcc aaagggcagc gtggaacagc atacccggca
     3241 ttacgtatat gcttcagatg acggttttga tcccaatttt tccacccaga aagctgacac
     3301 aacacgaatg atggtgcctt ttttccggca gttctgggat atgggcgcaa aggataaagc
     3361 gaccggaaaa tcacggagtg atgtgcagca acgcattcag cagtttcaca gtcaggaatt
     3421 tttaaactca ctccaaggca caacacaatt tgcgggtacc gattaccgca gcaaagacct
     3481 cacaccgaaa aaatcaaggc tactggctga caccatttca gctgtttatc tcgatggcta
     3541 tgaaggcaga cagtaagtgg atttaccata acgcgtcatt acgcgcaccg ctcagacggg
     3601 ctcagggcgt ttccggcggc tacgggataa aacaggcaac aacccccccg gaaaactgcc
     3661 ggaaacgcgc ccgataaatt ttatcctaat gcatgcctat gcacgcaact gcatggcata
     3721 tgttttgacg taaaatggat gccgtaagcc cttttttacc gactttctgc ttcatcagcc
     3781 tgactgacac gaccatattc tttcagaccg ccccctgcta aacttccttc atattgccgc
     3841 attaacccgg gaggctccgc aatgagcaac actacacact acgaaaacgc caactttctt
     3901 cgggaactgg ctgaaagcct gccccgcata ctgccggaag gtggcccgga caaagctgcg
     3961 ctgttgcagc gactggctaa cgaagaactg gcccaggcag agtatgaaga tcaggttcgc
     4021 gccaaagtga ccgccgcccg tgctgacacg cgtccgggta tgaccacgga acaactccgc
     4081 cagcgcctgc atggccgtta tcaggaactg cgcgatgccg tatgacgtgg aatggaagca
     4141 gggagccatc gaggatgtaa cgacgctgtt tgactatatc gcggaaaatt catccctgtg
     4201 ggatgcgcag aacgttaccg accgggttct cgctgcggcg gacaaactgg ccgacttccc
     4261 gcgactctat gaagtggact cacgctatgg cgaggacgtt cgccggatca gtctgatggg
     4321 acagcatgtc ctctatgatg ttgatgacca gaccagaaaa gtccgggttc tggccgtggt
     4381 cggccagcgc cagaatcccc atattgttct ctaataaccg gtgacgggga cgcaattcat
     4441 tgcgtggccg tcgccggttg gacaagcaca gcgcgtcagc gttttcccca cggccaccac
     4501 cggcgcggtg ttccggccgg ggtctctgct gtctccggtt tgtgttccag cagtctcatg
     4561 gactgcattt cctgccgtag cccggccacc tcctccctga gtgcttttat ctccgcgatc
     4621 aacgcgtcgg aggccatttt ttcagtctca cccgacagtc tcaactcgtc ccgctcgtga
     4681 gtctcatcct gtcttaactc accgtacacc cggataagct cgctggtatc caccacccgg
     4741 ccgccatcag cttccgtacg gtatgatacg cggcctttcg ccatatcccg atacagggaa
     4801 cttcgggctt tacccgtcag ttccgtcgcc tgtctcaccg tcacccacgc cattgtctca
     4861 ccccgctatt tttgagacag tatacctgac aggcgcacac catgcccgga aggaaagaat
     4921 catgacaaaa caggaaaaaa cagccctcaa tatggctaaa tttattaagg agcagtcgct
     4981 gctattgctc gataaactga atgagctgga tatggatacc ccggccgaca tgtgcgagcg
     5041 gctacacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaggaata
     5101 acagcaccgg catggccggt gctggcattt attttttgcc gaacggccac caccggcggg
     5161 caggtttgtc ctccggcagt tcaggtaccg gcacgttaaa atagcggctg ttatattccc
     5221 gcatcagctc cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc tcactcacca
     5281 cgtcaatcat cgcgttaatc tgctccggtg agcacttccc ggcatcaacc acctgcacac
     5341 cgttctcgat ggcgaaccgc tccaccttgc gatagaggta atcacacagc ttcattcctg
     5401 tgggtttgtt ccgttcccat tgcggatttt taccagtgaa aaagtacgtg gctttttcgt
     5461 cgctgttgag ccgctcgtca tacatcccga tggatttcat catcgccaga tgctgggccc
     5521 ggtcgacaaa acagaaaaca tacgccccgg tctgccaggc gagcaccacc atatcttcca
     5581 gatgctcgct ggtcggcatg atgcagcgct cgccgttccg gtcctccgcg tcttcaataa
     5641 ataccatgta cagcgccata atccctcact ccgttgtcat aaatttaaag cacgcaaaca
     5701 accgttcacg atccagctct gccgttttgc gggcctcgcc ggtctgtggg ttcacgattt
     5761 catccaccga caggattgcc cgctgcaccc tgacctgcgt cgcccggtcc gggcagatgt
     5821 accagatttc atcgatgccg ttagctttgc ggttgaacag atacccggac accacttcct
     5881 gataccggcg acggctcttg acggtgcgct ccacctcaaa cgccaccgtt ttaccgtcca
     5941 gctccgccag ggcgtccggc actttcatgc ctttaagact cagacggtgc agaatgcgcc
     6001 agccggtggc ccctctggct tccatatcaa ggcgcaggga ttgcacggcc agccggtgcg
     6061 gcagatgtgc tgccgacacc cttgacggct gaaacggaat aacatccgcc agcggttcgt
     6121 cctcgtcgaa cgagaacgcc acgccatgcg gcgtcagccc ccacacgctg acctgacggc
     6181 cgtccaccgg atatttggtc cggaccagca gccctttcgc caccaggctg tcgaggaagc
     6241 ggtagaccgg ctgcaccgcc gcaatcccca gccgccgcgc aaccagctcc gccgtggaac
     6301 acagctcttc ccgcaaccag cgcagggtca gccgggtttt ctccgcgttc cgcgcctgtc
     6361 gtttcgcgtg gctgacgttg tacagctcca gcgccatcag taaatccccg caaaatcatc
     6421 atccgctttc ggcccgccgc cgtcggccgg gggccggaac tccgccaggg gatcggtttt
     6481 cgccagcacc gggaaacgct gcaacgggat ttcccggcgc tcaacccgca gcggactgct
     6541 gaacgccatc cgggccacac ccagcccggt catgaccgcg aacccgtccg gcaggtgttg
     6601 cagggtgttc acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt ccccgctgat
     6661 atgctccacg ttccccgcct cccggctggc acggcggcgc tccacgtcca ccctgatttc
     6721 cccggtctgc cccgccgccc actgcgcgga ctccaggctg gccgcccggt aaaaccagcg
     6781 gatcggcgtg ttatccagta ccgtggtttt cacaaattca gcgggcagat cctgcccgca
     6841 ctgcccgaaa tcgcccagcg actggtgcgc gagcaacaga ttgcagtccc ggctgcgcag
     6901 cgtccccagt gcgttcagta catacttcga cagcagatat ttaatttcat cgagcatcac
     6961 gctgacgtgt ggccactccc ggaactcgtc ccgcgcaatg atgatttgcg cgcagcgggc
     7021 aaacagcatt ttttgcacac ggatcaccgc ctcgtcgtcc atcgagccga taacgtacag
     7081 gcagccgccg ccgttgataa tccccgccac atcgatgcca ctgtccgtct gcaggacgct
     7141 caggtcagcg acgttttcca gcctagtgat caaccccttc acgtcctttt tcagggcctc
     7201 cggcagcgcg tacgccgccg ccagcacctg ctgaatgttt gccccctgcg gaaactgctc
     7261 cgcaatcagc ttcgccgctt tctgctcgct gatgcggtag tggtccgcca cgtcgccggt
     7321 ctcgcccagg ttaagcccgg caatcagcag gtttttcagc gcgtactggt cgagatcgcg
     7381 gaacagattc agctgcgccg gtttccccgg ccgcagatcc agcagggtga agtccggaca
     7441 gtgcgcccgg aagacgtgcg gcgcccatgc gtcccctttg gggtcgaagt acaccaccgc
     7501 gtccccgaaa tcgcgtacgc agcgaatgag cgcgttggtg gccatcacgc tcttgccgct
     7561 cccgggcagg ccgaggatct gaatgttggt cttgcgccag gtatcacgcg gaaccagcac
     7621 cggcttgccc tgctcatcct tacccagaaa caccgcctgc tcctgccggg cctgctccga
     7681 ggaaaaatcg gccagcgcat taaccggtac cggctccccg aaatgcaggt ggcgcacgtc
     7741 ggtcatctct cctgtccgct ggctgctgcg ccggctctcc tgattcagtt tcgcgttcac
     7801 cgcctcgtca gcagccgcaa aatgaccgtc aagcagcagg aacgccagca gccgcgccaa
     7861 cacaatcccg acagccagcc cgcccgcgca ccaggcaatc ccgaacaggt tagccggctg
     7921 cagcaggtaa taatctgcgt ttgcgtagag gtccggacgc aggacggttt ctccccagct
     7981 ctcgataaac acggcgacaa ccagcccccc cagaagggcc agaccggccg cgccggtcag
     8041 ccagtacgcg tagttccaga catgtcgacg tgccctgacc atccgcccgg gcgaatccag
     8101 cgaggccagc accgaaaatg gcgctggccg tttattatcg cgcttatagc gatcggcata
     8161 ctcccgtcgc tccttatgac tcagccccac aaaacgcatc cgtccggccc aaagttcagc
     8221 cagaccgaaa aacaggccga taactaggaa agccccggcc agaccgtaat actcctggta
     8281 cagccagctg acccacggat aaatctgccg ggccagctcc ggaatgtgcc acttccaggc
     8341 aagcgaatcc gtattaaaca tgttctctct ccttcagacg atggtttgcc ggcgtgatgt
     8401 gcagcccccg ctgcacaagc cgcggacatg ccccaaggca tggcggcggc ggtcacgtcc
     8461 gtggtgattg cgttcagctt tcaccacgga ggccagcctc tttccgcccc atgcggaaaa
     8521 ggcgcagccc cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa ggcggcaccc
     8581 cagtgcggtc ttttccggtg ttccggcccc cgtcagagtt cgccatagcg agcggctgac
     8641 cacccgcaaa atcaggagca gctttgctgc ttcgccaggt gcgtccagca ccgtggattt
     8701 ttgtgggtgg tcaggcgtga gcctgacggg ggccgggtga atttctggcg tggccggcgt
     8761 ccagccggac ggagccagaa agagggggag agaccgtgcg tcggtggcgt ccagccagcg
     8821 taagcagggg atatccccct gaggtcagcc acgccctcgc ggggatgacc tgtatttctc
     8881 cgtaaggaga aaaaagcaac agagataggt taacgtaaaa tgttcagcga aaaaccgata
     8941 tgattcacct atcctggtaa tttattatca gggaatataa agacttaatc aggggacaat
     9001 atgccacatc agcgcgtccg gtgtccgtct tgtaggtgcc ggggaaattg cgcctcctcg
     9061 ctcactcggt cgctacgctc cggccgtgag actgcggcgg gcgctgcgga cacatgcaaa
     9121 gttacccaca gattccgtga ataagcaggg gactaacatg tgaggcaaaa cggcaaggcc
     9181 acgcaggtgg cgtttttcca taggctccgc cctcctgact gagttcacat aaacagacgc
     9241 tttttcggtg catctgtggg agccggtggc tcaaccatga atcagtcagg agtg
//
