LOCUS       CP052313                2789 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0102 plasmid pE16KP0102-4,
            complete sequence.
ACCESSION   CP052313
VERSION     CP052313.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609923
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:56:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,786
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,512
            CDSs (with protein)               :: 5,512
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 68 of 150
            Pseudo Genes (incomplete)         :: 77 of 150
            Pseudo Genes (internal stop)      :: 27 of 150
            Pseudo Genes (multiple problems)  :: 20 of 150
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0102"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0102-4"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(2502..2789,1..114))
                     /locus_tag="HJX35_28895"
     CDS             complement(join(2502..2789,1..114))
                     /locus_tag="HJX35_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJP02683.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(114..305)
                     /locus_tag="HJX35_28900"
     CDS             complement(114..305)
                     /locus_tag="HJX35_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJP02684.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFCTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            262..685
                     /locus_tag="HJX35_28905"
                     /pseudo=""
     CDS             262..685
                     /locus_tag="HJX35_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            710..841
                     /locus_tag="HJX35_28910"
     CDS             710..841
                     /locus_tag="HJX35_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJP02685.1"
                     /translation="MHEPPVQSDYHALFGNYRLESNPKRHDKSPLAVAIGRGCTRDY"
                     /name="replication initiation protein"
     gene            complement(1132..1551)
                     /locus_tag="HJX35_28915"
     CDS             complement(1132..1551)
                     /locus_tag="HJX35_28915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJP02686.1"
                     /translation="MKLSLFFISVITFLLAISPIAYSDNTIIPDRVYDPYRNSAVTPDG
                     TVYDRLTGGVLKASEHPQPNFGFPVQSSSDSCDTTYVKARTEDGRLLVTSDGVTLHVVD
                     NIVDVAHWQPNDELRVCGDRVINIDDNTSSSFTYN"
                     /name="hypothetical protein"
     gene            complement(1548..1745)
                     /locus_tag="HJX35_28920"
     CDS             complement(1548..1745)
                     /locus_tag="HJX35_28920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJP02687.1"
                     /translation="MERVLGIYVVGIIVSAGFLFIQEVRNGRRRSYTEEGASMLVGIIC
                     AFLWPLVLIAYFFDKKNGKL"
                     /name="hypothetical protein"
     Typing          529..1038
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.038"
                     /coverage="99.0"
                     /evalue="1.71e-152"
ORIGIN
        1 tgcattacgt tcaccgctac aggtggacag gtcacctgct ttaattaacc tgtgcagtgt
       61 tgttctactc ttacccgtca gtcgggcagc ctcactaata gacactctgg ccatcagtta
      121 ccatcctgta atgcatccag tctcgcggtc agtgtacaga aaagatgctc cgcatcatcg
      181 tggagtttct cacaaaggtc ggcctcgaca tcgagatcaa gctcgttaag cttctccagc
      241 aggagcagag actgattctg gatgaattta gccatgttca gcgctgtttt ctgttgtttc
      301 gtcataaagg gcctcagagc gtcagtgctc aatgtcagac agatctcttt tccgcttcct
      361 cgctcactca gtcgctacgc tcctgccgtt cggctgcggc gagcggtcgc aacgcgcaca
      421 taacgacata gagcagacag ctcggagagg gagaaggcag cacagcggcc gtttttccat
      481 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
      541 ccgacaggac tataaagata ccaggcgttt ccccctgatg gctccctcgt gcgctctcct
      601 gttcctgcca ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
      661 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
      721 ccccgttcag tccgactacc acgctctttt cggtaactat cgtcttgagt ccaacccgaa
      781 aaggcacgac aaatcgccat tggcggtagc cattggtaga gggtgtacaa gagattactg
      841 atgcaaagtt cttgaagtta gagccagact gcggctaaac tgaaaggacg aatttggtgg
      901 cagcggtctt gtacaccctg ttaccacggt tcagaagttc cccaacttac tgaaccttcg
      961 aaaaaccacc tccccaggtg gttttttcgt tttcagagca agagattacg acgctcctga
     1021 aaggatctca agaagatcct tttgaacttt ggttcatttc ttcggctatc cccccataca
     1081 tgctcgtcaa tctttctatc atcaccatag atattctttg aataaaagag gttaattgta
     1141 tgtgaacgag gaggaagtat tgtcatcaat attgattact ctatcgccac agactctaag
     1201 ctcatcattt ggttgccagt gagcaacatc aacaatattg tcaactacat gcagagttac
     1261 tccgtcgctc gtgactaaaa gcctaccgtc ttctgtccta gctttaacat acgttgtatc
     1321 acaactatcc gatgaggatt gaacaggaaa cccaaaatta ggttgagggt gctctgatgc
     1381 cttcaaaact cctcctgtca gacggtcata aacggtgcca tcaggagtta cagccgaatt
     1441 tctataagga tcatatactc tgtcaggaat aatcgtgtta tctgagtatg ctatcggtga
     1501 tatagccaat aaaaaagtaa tgaccgagat gaaaaacaga cttaacttca taatttccca
     1561 ttttttttat caaaaaaata tgcaattaaa actaaaggcc acaaaaacgc acaaattatg
     1621 ccgacaagca ttgacgcacc ttcttcggtg taacttctgc gcctcccgtt cctaacttct
     1681 tggataaaca ggaaaccggc actcacaata ataccgacaa cataaattcc taataccctt
     1741 tccataaaac attccgttct tatcagatta aacataacat cccttgtacg caccgctaaa
     1801 acggtctcag ggcgtttctg aggggaaacc ggtaaaacag gcaacaaacc acccgaaaac
     1861 ccgccagaaa cgcgcaggct ctgtttttaa ggcatgcatg agtatgcacg caagtgcata
     1921 gcgcgaaaat tcacgccatt cagccagagt aagtcctgtt tccggcgttc tttccatccg
     1981 aaaacggtga acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg
     2041 aaaaccggtt ttcagcacgt tcaggaatcg ggttcgtcga cggccggcaa tcacgttatg
     2101 gcaagtcgcc ggacgactca ccacacgatt caccggcgat tcgaatcgtg aattgaatca
     2161 agagtcacca tgagcaaaaa acacccaaag tgatgaattg aatcaagagt cgattcatca
     2221 taacaatatg ataataaagc gtgattcaca tatgaatcgt gggcaattcg tcagcgattc
     2281 gccatagagt cgtccacgac ttggatctgc actttgtaac acatcaccgc acatttattt
     2341 ttcattaacc cattgttgta aataaggaat ctgcaaaatt ttaattttgc gtggggtgtg
     2401 ggtattttta gtggtgagga gcgttcagag atctgacgat ttttgggaag gttcaggaac
     2461 tccctgggat ggccggagag gaaaactgaa gcatgacggg gtcaggattt tttccagaat
     2521 ttccaccatg gcgccgggga ttcagttaat tttgcctggt gattttcaag aaggagcatg
     2581 gcctgtttca ggctgtcaat gtgcgaatcc ttggacaaaa gtaaggtgtt caggtgttca
     2641 atctcctgtt tcagatgacg aatcacctgt tcactctggt cagcagaggc tgtatcacgc
     2701 tgttcagaga cctgtccaga atggtgttca cccttcagtt gttcaaaggg gccaaagaca
     2761 cgtaaaagct ctgaagtatc taccatttt
//
