LOCUS       CP052308                4167 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0133 plasmid pE16KP0133-2,
            complete sequence.
ACCESSION   CP052308
VERSION     CP052308.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609926
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:44:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,397
            Genes (coding)                    :: 5,298
            CDSs (with protein)               :: 5,298
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 44 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0133"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0133-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(294..926)
                     /locus_tag="HJX38_27565"
     CDS             complement(294..926)
                     /locus_tag="HJX38_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO16871.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="hypothetical protein"
     gene            complement(926..2041)
                     /locus_tag="HJX38_27570"
     CDS             complement(926..2041)
                     /locus_tag="HJX38_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QJO16872.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     gene            complement(2386..2499)
                     /locus_tag="HJX38_27575"
                     /pseudo=""
     CDS             complement(2386..2499)
                     /locus_tag="HJX38_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2524..2958)
                     /locus_tag="HJX38_27580"
                     /pseudo=""
     CDS             complement(2524..2958)
                     /locus_tag="HJX38_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            2915..3106
                     /locus_tag="HJX38_27585"
     CDS             2915..3106
                     /locus_tag="HJX38_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO16873.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3106..3507
                     /locus_tag="HJX38_27590"
     CDS             3106..3507
                     /locus_tag="HJX38_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO16874.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3853..4095)
                     /locus_tag="HJX38_27595"
     CDS             complement(3853..4095)
                     /locus_tag="HJX38_27595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO16875.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(2387..2794)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 cctgttttac cggtttcccg tcagaaacgt cctgagtccg ttttagcggt gcgtacaatt
       61 atgattttat gttaaattgt tttaagtgaa tagagagaat ataacttaag ggagcatctt
      121 gtgctgggtc ttttacatta gatctgcaca atcattgttt tggcatctgg tgatgtcttt
      181 aggacgtttg agattgctga taactaaagt gtatgatgct ttctatacgt tatccggcgt
      241 ttaataacac cctggtgata agccagcccg tgaagggctg gcttgatttg gatttatatt
      301 tttgaaataa aatcttttat ttcatcaaag caattgtctg ctgtgtttat tattccattt
      361 ttcatggata ttcctattct tgagtcggat tctctattgc agctaagata gacttgtacc
      421 ttagcttttt tttcgtttgt tggataagtg tactctttgt cactcgctga gatgtcatct
      481 ttattatttt taattttttc gaaaaattct attatgtatt catataacgc tcgagtgctt
      541 gcgtgttcaa ttatcagatc ttcaatggca ccttgcttgt cttttcctgg cattataaag
      601 aaagaaattt ttgtattgcc attcatagct atctcgttat gcgagctggg ggaggagatt
      661 tgtagttctc gcaatgtggc aattattgat ctttctgtgt cagaaaatga tgtgtcagca
      721 tctaatataa taaaaatact ttctttttca tcgaaagaat caatgttttt gaatgctcta
      781 agtgcttgtt ttaattttga ttttccattt attttttcga caaaaacatc ggtaatgcct
      841 tgtatttcaa gtaggttgca tagaaaatga aaatcatcat caccctcaac gaaaatgatc
      901 tttttgctat tatttatgtc tttcattatc ttacctccca gtcgttctca actgagtagg
      961 agaaatcatc catattgaat tgtacggctg ttggttttag ggagttcttt tctcttccta
     1021 gtctaatata agcgatatct tcatttttgc tatctatttc gtttattgct cgtatcacat
     1081 cttgactatg agttgttgca aatatttgat tgttgttttg ttttgcagat gaaattaatg
     1141 attttattat gtctttaatt actgagtaat gtattccgtt ctcgatttca tcaataagaa
     1201 taattgagtt ttcctgtacc agcactgagc tgataaaaga taatgctcga ctgataccct
     1261 cgccaagcat tgatatttca tttagctcac taaatccaat gtctaaatat atttccttat
     1321 tgttacctat cgcagatatt gctatatcta caattctgtc atctattttt tttaagtttt
     1381 ctagtatgtc ttttttcctt ttttgtgtta gtaggctaga tacatttgta attgttgcct
     1441 ctttgtttat ctttctggat gttgttatta ttgttgctgt cttaaattta taattaatcc
     1501 catccctttc gttttttatt tcggatttta tttctacgcc atcttctttc aattgcatag
     1561 agaaaagctt attggaaacc ctgtttttcg cattgggtgt ctcggtcaca tttattttta
     1621 aagagtttac tgtggcctga gaaccgatag tttgacgagg tatgttttgg ttagatagta
     1681 cggtgtcaac gtttagagac aaacttgatt ctaatctctg atttgtttct gtttcatatg
     1741 tttgtgttac ttcagagcgt tcatctcgaa tgactatgga catcttttgt gatgtgtcca
     1801 tatcatgaaa gtaactaacc caaaatggct tgtttttgtt tatttctgcc atttcttttc
     1861 tgaatgcttg aatgtttaac aaaacagccg ggttttttac atcataaaaa caaaaaatag
     1921 catccagtat agatgttttc ccgtagttgt ttttcccagc tatgatattt aaagtcttta
     1981 attgctttag atatagctca ttgaagcatt tgaagttctt taaacttatt tcctttatca
     2041 tgatagcctc tgttcgtgtt tttgttatat gccatacgat ttgttagtac cttaataaaa
     2101 tgtacagctt tcattgtgat tagctagact ttatgtcaaa gattaacttt gattgcttgt
     2161 gttttaatcg atttttttta ctttcttctt gagattatct ggcagcagcc gtaagcttcc
     2221 ggctgtgaac gaaaaaaacc gccttggcgg gcggtttttt cgaaaggttc agtagagttg
     2281 gcgctctatg aaccgaggta actggcttaa agggagcgca gtcaccaaaa ctgttcgttt
     2341 agtttagctt taactggcgt ataacttcaa gactaactcc ctaaatcagt taccagtggc
     2401 tgctgccagt ggcgctttgt cgtgtctttc cgggttggac tcaagacgat agttaccgga
     2461 ttaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     2521 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa
     2581 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca tcagggggaa
     2641 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattc
     2701 cgtgatgctc gtcagggggg cggagcctat ggaaaaacgg ccgctgtgcg gcctttcctt
     2761 ctttcctcca tgcgattact cggttttctc gagcgtttct acgcatgctc ctgccgctcg
     2821 ccgcagccga acggcaggag cgtagcgact gagtgagcga ggaagcggaa aagaggagat
     2881 ccctacactg accactgacg ctctgaggcc tcttatgacg aaacaacaga aaacagcgct
     2941 gaacatggct aaattcatcc agaatcagtc tctgctcctg ctggagaagc ttaacgagct
     3001 tgatctcgat gtcgaggccg acctttgtga gaaactccac gatgatgcgg agcatctttt
     3061 ccgtacactg tcctcgagac tggatgcatt acaggatggt aactgatggc cagagtgtct
     3121 attagtgagg ctgcccgact gacgggtaag agtagaacaa cactgcacag gttaattaaa
     3181 gcaggtgacc tgtccacctg tagcggtgag cgcaatgcaa aaatggtaga tacttcagag
     3241 cttttacgtg tctttggccc ctttgagcaa ccgaagggtg aacaccattc tggacaggtc
     3301 tctggacagc gtgatacagc ctctgctgac cagagtgaac aggtgatccg tcatctgaaa
     3361 caggagattg aacacctgaa caccttactt ttgtccaagg attcgcacat tgacagcctg
     3421 aaacaggcca tgctccttct tgaaaatcac caggcaaaat taactgaatc cccggcgcca
     3481 tggtggaaat tctggaaaaa atcctgaccc cgtcatgctt cagttttcct ctccggccat
     3541 cccagggagt tcctgaacct tcccaaaaat cgtcagatct ctgaacgctc ctcaccacta
     3601 aaaataccca caccccacgc aaaattaaaa ttttgcagat tccttattta caacaatggg
     3661 ttaatgaaaa ataaatgtgc ggtgatgtgt tacaaagtgc agatccaagt cgtggacgac
     3721 tctatggcga atcgctgacg aattgcccac gattcatatg tgaatcacgc tttattatca
     3781 tattgttatg atgaatcgac tcttgattca attcatcact ttgggtgttt tttgctcatg
     3841 gtgactcttg attcaattca cgattcgaat cgccggtgaa tcgtgtggtg agtcgtccgg
     3901 cgacttgcca taacgtgatt gccggccgtc gacgaacccg attcctgaag gtgctgaaaa
     3961 ccggttttcc ggctgcgccg gcaacgcttc acggccacgc aataaattgc gtccccgttc
     4021 accgttttcg gaggagaaga acgccggaaa caggacttcc cctggctgaa tggtgtgaat
     4081 ttccgcgcta tgcacttgcg cgcatactca tgcatgccgt aaaaacagag cctgcgcgtt
     4141 tctggcgggt tttcgggtgg tttgttg
//
