LOCUS       CP052305                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0172 plasmid pE16KP0172-2,
            complete sequence.
ACCESSION   CP052305
VERSION     CP052305.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609927
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:02:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,482
            CDSs (total)                      :: 5,355
            Genes (coding)                    :: 5,217
            CDSs (with protein)               :: 5,217
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 72 of 138
            Pseudo Genes (incomplete)         :: 64 of 138
            Pseudo Genes (internal stop)      :: 27 of 138
            Pseudo Genes (multiple problems)  :: 23 of 138
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0172"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0172-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(7657..9294,1..48))
                     /locus_tag="HJX39_27360"
     CDS             complement(join(7657..9294,1..48))
                     /locus_tag="HJX39_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ46385.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            451..870
                     /gene="exc2"
                     /locus_tag="HJX39_27365"
     CDS             451..870
                     /gene="exc2"
                     /locus_tag="HJX39_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJJ46375.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            1176..1439
                     /locus_tag="HJX39_27370"
     CDS             1176..1439
                     /locus_tag="HJX39_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJJ46376.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            1429..1728
                     /locus_tag="HJX39_27375"
     CDS             1429..1728
                     /locus_tag="HJX39_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJJ46377.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1790..2167)
                     /locus_tag="HJX39_27380"
     CDS             complement(1790..2167)
                     /locus_tag="HJX39_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJJ46378.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            2236..2415
                     /locus_tag="HJX39_27385"
     CDS             2236..2415
                     /locus_tag="HJX39_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ46379.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2443..2973)
                     /locus_tag="HJX39_27390"
     CDS             complement(2443..2973)
                     /locus_tag="HJX39_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ46380.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2980..3711)
                     /locus_tag="HJX39_27395"
     CDS             complement(2980..3711)
                     /locus_tag="HJX39_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJJ46381.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(3711..5675)
                     /locus_tag="HJX39_27400"
     CDS             complement(3711..5675)
                     /locus_tag="HJX39_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJJ46382.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7156..7305)
                     /locus_tag="HJX39_27405"
     CDS             complement(7156..7305)
                     /locus_tag="HJX39_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJJ46383.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7390..7647)
                     /locus_tag="HJX39_27410"
     CDS             complement(7390..7647)
                     /locus_tag="HJX39_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ46384.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     BGC             2657..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6475..7053
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2984..3711)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gtgtcccaga cctgaattac ccggacctcg tccgtctccg ccactcatat acttttcctc
       61 tttgactttt aaaacaataa actaaaataa aatactgtac atataaccac tggttttata
      121 tacagtataa aaattacgct gccttgtttt tcatgtcaag tctgtaaatt tgcgtttttg
      181 tgaaaatgat caaaaaaaca gccactcaca ccatgcgcaa tcccctccag atgcaacaaa
      241 aaccagcaca aacggcgccg ttctccggcc tcagtatgtt ttactgaaat cattatatga
      301 gataccagaa agccccctcc gggacaggaa aagaaacggg gggtctggtt cgggttgttc
      361 ggtcactgaa cacggtcaga aactgatgat aagatagcct gcggatatat aactcaatga
      421 gaaaaaagaa ttaaaggtaa ggagacgctc atgagacgtt ccggcagcaa actgtgtctt
      481 atcgccatga taacagcgac agcaactctc actgcctgtg ctccaaaggg cagcgtggaa
      541 cagcataccc ggcattacgt atatgcttca gatgacggtt ttgatcccaa tttttccacc
      601 cagaaagctg acacaacacg aatgatggtg ccttttttcc ggcagttctg ggatatgggc
      661 gcaaaggata aagcgaccgg aaaatcacgg agtgatgtgc agcaacgcat tcagcagttt
      721 cacagtcagg aatttttaaa ctcactccaa ggcacaacac aatttgcggg taccgattac
      781 cgcagcaaag acctcacacc gaaaaaatca aggctactgg ctgacaccat ttcagctgtt
      841 tatctcgatg gctatgaagg cagacagtaa gtggatttac cataacgcgt cattacgcgc
      901 accgctcaga cgggctcagg gcgtttccgg cggctacggg ataaaacagg caacaacccc
      961 cccggaaaac tgccggaaac gcgcccgata aattttatcc taatgcatgc ctatgcacgc
     1021 aactgcatgg catatgtttt gacgtaaaat ggatgccgta agcccttttt taccgacttt
     1081 ctgcttcatc agcctgactg acacgaccat attctttcag accgccccct gctaaacttc
     1141 cttcatattg ccgcattaac ccgggaggct ccgcaatgag caacactaca cactacgaaa
     1201 acgccaactt tcttcgggaa ctggctgaaa gcctgccccg catactgccg gaaggtggcc
     1261 cggacaaagc tgcgctgttg cagcgactgg ctaacgaaga actggcccag gcagagtatg
     1321 aagatcaggt tcgcgccaaa gtgaccgccg cccgtgctga cacgcgtccg ggtatgacca
     1381 cggaacaact ccgccagcgc ctgcatggcc gttatcagga actgcgcgat gccgtatgac
     1441 gtggaatgga agcagggagc catcgaggat gtaacgacgc tgtttgacta tatcgcggaa
     1501 aattcatccc tgtgggatgc gcagaacgtt accgaccggg ttctcgctgc ggcggacaaa
     1561 ctggccgact tcccgcgact ctatgaagtg gactcacgct atggcgagga cgttcgccgg
     1621 atcagtctga tgggacagca tgtcctctat gatgttgatg accagaccag aaaagtccgg
     1681 gttctggccg tggtcggcca gcgccagaat ccccatattg ttctctaata accggtgacg
     1741 gggacgcaat tcattgcgtg gccgtcgccg gttggacaag cacagcgcgt cagcgttttc
     1801 cccacggcca ccaccggcgc ggtgttccgg ccggggtctc tgctgtctcc ggtttgtgtt
     1861 ccagcagtct catggactgc atttcctgcc gtagcccggc cacctcctcc ctgagtgctt
     1921 ttatctccgc gatcaacgcg tcggaggcca ttttttcagt ctcacccgac agtctcaact
     1981 cgtcccgctc gtgagtctca tcctgtctta actcaccgta cacccggata agctcgctgg
     2041 tatccaccac ccggccgcca tcagcttccg tacggtatga tacgcggcct ttcgccatat
     2101 cccgatacag ggaacttcgg gctttacccg tcagttccgt cgcctgtctc accgtcaccc
     2161 acgccattgt ctcaccccgc tatttttgag acagtatacc tgacaggcgc acaccatgcc
     2221 cggaaggaaa gaatcatgac aaaacaggaa aaaacagccc tcaatatggc taaatttatt
     2281 aaggagcagt cgctgctatt gctcgataaa ctgaatgagc tggatatgga taccccggcc
     2341 gacatgtgcg agcggctaca cgaggatgcg gaaacactct ggcaggtgat gcgggataag
     2401 ctcggggagg aataacagca ccggcatggc cggtgctggc atttattttt tgccgaacgg
     2461 ccaccaccgg cgggcaggtt tgtcctccgg cagttcaggt accggcacgt taaaatagcg
     2521 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
     2581 ccgctcactc accacgtcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc
     2641 aaccacctgc acaccgttct cgatggcgaa ccgctccacc ttgcgataga ggtaatcaca
     2701 cagcttcatt cctgtgggtt tgttccgttc ccattgcgga tttttaccag tgaaaaagta
     2761 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
     2821 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
     2881 caccatatct tccagatgct cgctggtcgg catgatgcag cgctcgccgt tccggtcctc
     2941 cgcgtcttca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
     3001 aaagcacgca aacaaccgtt cacgatccag ctctgccgtt ttgcgggcct cgccggtctg
     3061 tgggttcacg atttcatcca ccgacaggat tgcccgctgc accctgacct gcgtcgcccg
     3121 gtccgggcag atgtaccaga tttcatcgat gccgttagct ttgcggttga acagataccc
     3181 ggacaccact tcctgatacc ggcgacggct cttgacggtg cgctccacct caaacgccac
     3241 cgttttaccg tccagctccg ccagggcgtc cggcactttc atgcctttaa gactcagacg
     3301 gtgcagaatg cgccagccgg tggcccctct ggcttccata tcaaggcgca gggattgcac
     3361 ggccagccgg tgcggcagat gtgctgccga cacccttgac ggctgaaacg gaataacatc
     3421 cgccagcggt tcgtcctcgt cgaacgagaa cgccacgcca tgcggcgtca gcccccacac
     3481 gctgacctga cggccgtcca ccggatattt ggtccggacc agcagccctt tcgccaccag
     3541 gctgtcgagg aagcggtaga ccggctgcac cgccgcaatc cccagccgcc gcgcaaccag
     3601 ctccgccgtg gaacacagct cttcccgcaa ccagcgcagg gtcagccggg ttttctccgc
     3661 gttccgcgcc tgtcgtttcg cgtggctgac gttgtacagc tccagcgcca tcagtaaatc
     3721 cccgcaaaat catcatccgc tttcggcccg ccgccgtcgg ccgggggccg gaactccgcc
     3781 aggggatcgg ttttcgccag caccgggaaa cgctgcaacg ggatttcccg gcgctcaacc
     3841 cgcagcggac tgctgaacgc catccgggcc acacccagcc cggtcatgac cgcgaacccg
     3901 tccggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag
     3961 ctgtccccgc tgatatgctc cacgttcccc gcctcccggc tggcacggcg gcgctccacg
     4021 tccaccctga tttccccggt ctgccccgcc gcccactgcg cggactccag gctggccgcc
     4081 cggtaaaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa ttcagcgggc
     4141 agatcctgcc cgcactgccc gaaatcgccc agcgactggt gcgcgagcaa cagattgcag
     4201 tcccggctgc gcagcgtccc cagtgcgttc agtacatact tcgacagcag atatttaatt
     4261 tcatcgagca tcacgctgac gtgtggccac tcccggaact cgtcccgcgc aatgatgatt
     4321 tgcgcgcagc gggcaaacag cattttttgc acacggatca ccgcctcgtc gtccatcgag
     4381 ccgataacgt acaggcagcc gccgccgttg ataatccccg ccacatcgat gccactgtcc
     4441 gtctgcagga cgctcaggtc agcgacgttt tccagcctag tgatcaaccc cttcacgtcc
     4501 tttttcaggg cctccggcag cgcgtacgcc gccgccagca cctgctgaat gtttgccccc
     4561 tgcggaaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc
     4621 gccacgtcgc cggtctcgcc caggttaagc ccggcaatca gcaggttttt cagcgcgtac
     4681 tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag atccagcagg
     4741 gtgaagtccg gacagtgcgc ccggaagacg tgcggcgccc atgcgtcccc tttggggtcg
     4801 aagtacacca ccgcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc
     4861 acgctcttgc cgctcccggg caggccgagg atctgaatgt tggtcttgcg ccaggtatca
     4921 cgcggaacca gcaccggctt gccctgctca tccttaccca gaaacaccgc ctgctcctgc
     4981 cgggcctgct ccgaggaaaa atcggccagc gcattaaccg gtaccggctc cccgaaatgc
     5041 aggtggcgca cgtcggtcat ctctcctgtc cgctggctgc tgcgccggct ctcctgattc
     5101 agtttcgcgt tcaccgcctc gtcagcagcc gcaaaatgac cgtcaagcag caggaacgcc
     5161 agcagccgcg ccaacacaat cccgacagcc agcccgcccg cgcaccaggc aatcccgaac
     5221 aggttagccg gctgcagcag gtaataatct gcgtttgcgt agaggtccgg acgcaggacg
     5281 gtttctcccc agctctcgat aaacacggcg acaaccagcc cccccagaag ggccagaccg
     5341 gccgcgccgg tcagccagta cgcgtagttc cagacatgtc gacgtgccct gaccatccgc
     5401 ccgggcgaat ccagcgaggc cagcaccgaa aatggcgctg gccgtttatt atcgcgctta
     5461 tagcgatcgg catactcccg tcgctcctta tgactcagcc ccacaaaacg catccgtccg
     5521 gcccaaagtt cagccagacc gaaaaacagg ccgataacta ggaaagcccc ggccagaccg
     5581 taatactcct ggtacagcca gctgacccac ggataaatct gccgggccag ctccggaatg
     5641 tgccacttcc aggcaagcga atccgtatta aacatgttct ctctccttca gacgatggtt
     5701 tgccggcgtg atgtgcagcc cccgctgcac aagccgcgga catgccccaa ggcatggcgg
     5761 cggcggtcac gtccgtggtg attgcgttca gctttcacca cggaggccag cctctttccg
     5821 ccccatgcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc gtgtgagagg
     5881 aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag agttcgccat
     5941 agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgcc aggtgcgtcc
     6001 agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga cgggggccgg gtgaatttct
     6061 ggcgtggccg gcgtccagcc ggacggagcc agaaagaggg ggagagaccg tgcgtcggtg
     6121 gcgtccagcc agcgtaagca ggggatatcc ccctgaggtc agccacgccc tcgcggggat
     6181 gacctgtatt tctccgtaag gagaaaaaag caacagagat aggttaacgt aaaatgttca
     6241 gcgaaaaacc gatatgattc acctatcctg gtaatttatt atcagggaat ataaagactt
     6301 aatcagggga caatatgcca catcagcgcg tccggtgtcc gtcttgtagg tgccggggaa
     6361 attgcgcctc ctcgctcact cggtcgctac gctccggccg tgagactgcg gcgggcgctg
     6421 cggacacatg caaagttacc cacagattcc gtgaataagc aggggactaa catgtgaggc
     6481 aaaacggcaa ggccacgcag gtggcgtttt tccataggct ccgccctcct gactgagttc
     6541 acataaacag acgctttttc ggtgcatctg tgggagccgg tggctcaacc atgaatcagt
     6601 caggagtggc gaaacccgac aggacttaaa gatcatcacc gtttccgatg aatcgctccc
     6661 tcttgcgctc tcctgttcca accctgccgt ttcccggata cctgttccgc ctttctccct
     6721 tgcgggaagt gtggcgcttt ctcatagctc acactctggt atctcggctc ggtgtaggtc
     6781 gttcgctcca agctgggctg taagcaagaa ccccccgttc agcccgactg ctgcgcctta
     6841 tccggtaact gttcacttga gtccaacccg gaaaagcacg caaaacgcca ctggcagcag
     6901 ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa
     6961 gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag
     7021 ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc
     7081 aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag
     7141 atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaggagg acgatggtgc
     7201 caccgttcca ccctgaacat cccggatata gtttgcctga catgcgacca gaagaaagcc
     7261 tgaaaaaatc aatataaaaa gaaaaacaac ctttaccttt ttcatatacc ttccctgtat
     7321 ttaaaaaccc cggtattcat ccctgaacac cggaagtctg tatttcaaat catccttgat
     7381 aacttttgct taccagttac catcccggta aacaaaggat acaaagtaat caaatttact
     7441 aatatcgatt acatttttaa agtgtggctg taatatcgaa acccatgatt tttcaacatc
     7501 aaaccaacca ttattaatat tatcctttaa aggcatcccc agactttcaa tgacagaacc
     7561 atcatcaccg aagtcttttg agtattcacc acctttaaac tcttcggttt tcttatcaaa
     7621 ccaatgaata tgtaatttaa gccccatatt tacctcttaa agatattttt taatgtttcg
     7681 ttttggatcc ggccctttaa cctgcttacc cgtttttgga tcgaatgccc cgaggtgttc
     7741 gccatcactg gcgcggtacc cttcaagctc accgtgctgg gaatcccatt cataaatttt
     7801 acgcttttta tcaccatacc atcgggcccg cttaccacca ccaccttgtt taggggtttt
     7861 aggtttaccc tctttcaact ctcccaaccc cttaatatct tcagtcttgg gatcaggaaa
     7921 ataatcatgg ccgtaatctt tagttccctt acgaggtttt ttcttttcct catttaattt
     7981 attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggaaag
     8041 cgcagcctta tccctggata aggattcatt catttcgttt aaagatgtct gggcagcttt
     8101 taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat cctgccaccc
     8161 ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg cctctgcctc
     8221 ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggatcg ccttctctgt
     8281 cgatgcaatg cgactgttta aggtcgcaat atttttatct tcagcatcca gctcagcttt
     8341 cgctttatca tactctcttt tcagcccctc ttccgggtga gcagcgtccc atgcctgctg
     8401 gcgacgcttt tcttcctcca gacgctgttt tacctgtgcc ggagtaagaa catccgtcac
     8461 cgacacatag accggtttct gtccgctctc tttcgggaaa cgaataacgg cctcacggga
     8521 gttaccaccg gccgtaaaac cagccggacg tgaatcacct ttttcagcaa taatgccttt
     8581 tggcggggct ttcgcggccg gaacaccttt aggtacgctc acctgcagag acgggagacc
     8641 cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacaggaac
     8701 actcattggc cggccggtga caaccgcaat atgctgccgc tcgtccttca caatatccgc
     8761 cacacgttta gtgacgctga ccgtcgcctg gtccagcggc agggagctga ccggcgtctc
     8821 cgttaccgtg tctgccggca atgacgtcac gatttttgac atcattttcg gatcatcttt
     8881 tgctatctca gaaggcagca ctccgtaaat cgcaatcccc cacagaccaa acttaaacgg
     8941 acctttcagg gcagccagca catcagcaac agcggccgac aacgcatcgc cggaaacgga
     9001 taaagccagt ccttcagccc ccggagtggc cagggcaggc agaccgaagg ccatcgcggt
     9061 ggccgaagac tgtccgccac cagagctccc gctatgattg ccgctgttac cgccattacc
     9121 accgggttta ctcccgccgt taccaaactc ccccgggtta tagttatgaa tatccccgtt
     9181 aggtgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga
     9241 agggccacct ttacctgatg taccgttcac attcccactg gcctgaccac catt
//
