LOCUS       CP052300                4167 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0204 plasmid pE16KP0204-3,
            complete sequence.
ACCESSION   CP052300
VERSION     CP052300.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609929
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:41:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,394
            Genes (coding)                    :: 5,295
            CDSs (with protein)               :: 5,295
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 22 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0204"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0204-3"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(3565..4167,1..513)
                     /locus_tag="HJX41_27550"
     CDS             join(3565..4167,1..513)
                     /locus_tag="HJX41_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QJO64850.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     gene            513..1145
                     /locus_tag="HJX41_27555"
     CDS             513..1145
                     /locus_tag="HJX41_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO64851.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="hypothetical protein"
     gene            1511..1753
                     /locus_tag="HJX41_27560"
     CDS             1511..1753
                     /locus_tag="HJX41_27560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO64852.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2099..2500)
                     /locus_tag="HJX41_27565"
     CDS             complement(2099..2500)
                     /locus_tag="HJX41_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO64853.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2500..2691)
                     /locus_tag="HJX41_27570"
     CDS             complement(2500..2691)
                     /locus_tag="HJX41_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO64854.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2648..3082
                     /locus_tag="HJX41_27575"
                     /pseudo=""
     CDS             2648..3082
                     /locus_tag="HJX41_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            3107..3220
                     /locus_tag="HJX41_27580"
                     /pseudo=""
     CDS             3107..3220
                     /locus_tag="HJX41_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          2813..3220
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gcaacaatta caaatgtatc tagcctacta acacaaaaaa ggaaaaaaga catactagaa
       61 aacttaaaaa aaatagatga cagaattgta gatatagcaa tatctgcgat aggtaacaat
      121 aaggaaatat atttagacat tggatttagt gagctaaatg aaatatcaat gcttggcgag
      181 ggtatcagtc gagcattatc ttttatcagc tcagtgctgg tacaggaaaa ctcaattatt
      241 cttattgatg aaatcgagaa cggaatacat tactcagtaa ttaaagacat aataaaatca
      301 ttaatttcat ctgcaaaaca aaacaacaat caaatatttg caacaactca tagtcaagat
      361 gtgatacgag caataaacga aatagatagc aaaaatgaag atatcgctta tattagacta
      421 ggaagagaaa agaactccct aaaaccaaca gccgtacaat tcaatatgga tgatttctcc
      481 tactcagttg agaacgactg ggaggtaaga taatgaaaga cataaataat agcaaaaaga
      541 tcattttcgt tgagggtgat gatgattttc attttctatg caacctactt gaaatacaag
      601 gcattaccga tgtttttgtc gaaaaaataa atggaaaatc aaaattaaaa caagcactta
      661 gagcattcaa aaacattgat tctttcgatg aaaaagaaag tatttttatt atattagatg
      721 ctgacacatc attttctgac acagaaagat caataattgc cacattgcga gaactacaaa
      781 tctcctcccc cagctcgcat aacgagatag ctatgaatgg caatacaaaa atttctttct
      841 ttataatgcc aggaaaagac aagcaaggtg ccattgaaga tctgataatt gaacacgcaa
      901 gcactcgagc gttatatgaa tacataatag aatttttcga aaaaattaaa aataataaag
      961 atgacatctc agcgagtgac aaagagtaca cttatccaac aaacgaaaaa aaagctaagg
     1021 tacaagtcta tcttagctgc aatagagaat ccgactcaag aataggaata tccatgaaaa
     1081 atggaataat aaacacagca gacaattgct ttgatgaaat aaaagatttt atttcaaaaa
     1141 tataaatcca aatcaagcca gcccttcacg ggctggctta tcaccagggt gttattaaac
     1201 gccggataac gtatagaaag catcatacac tttagttatc agcaatctca aacgtcctaa
     1261 agacatcacc agatgccaaa acaatgattg tgcagatcta atgtaaaaga cccagcacaa
     1321 gatgctccct taagttatat tctctctatt cacttaaaac aatttaacat aaaatcataa
     1381 ttgtacgcac cgctaaaacg gactcaggac gtttctgacg ggaaaccggt aaaacaggca
     1441 acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg tttttacggc atgcatgagt
     1501 atgcgcgcaa gtgcatagcg cggaaattca caccattcag ccaggggaag tcctgtttcc
     1561 ggcgttcttc tcctccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc
     1621 gttgccggcg cagccggaaa accggttttc agcaccttca ggaatcgggt tcgtcgacgg
     1681 ccggcaatca cgttatggca agtcgccgga cgactcacca cacgattcac cggcgattcg
     1741 aatcgtgaat tgaatcaaga gtcaccatga gcaaaaaaca cccaaagtga tgaattgaat
     1801 caagagtcga ttcatcataa caatatgata ataaagcgtg attcacatat gaatcgtggg
     1861 caattcgtca gcgattcgcc atagagtcgt ccacgacttg gatctgcact ttgtaacaca
     1921 tcaccgcaca tttatttttc attaacccat tgttgtaaat aaggaatctg caaaatttta
     1981 attttgcgtg gggtgtgggt atttttagtg gtgaggagcg ttcagagatc tgacgatttt
     2041 tgggaaggtt caggaactcc ctgggatggc cggagaggaa aactgaagca tgacggggtc
     2101 aggatttttt ccagaatttc caccatggcg ccggggattc agttaatttt gcctggtgat
     2161 tttcaagaag gagcatggcc tgtttcaggc tgtcaatgtg cgaatccttg gacaaaagta
     2221 aggtgttcag gtgttcaatc tcctgtttca gatgacggat cacctgttca ctctggtcag
     2281 cagaggctgt atcacgctgt ccagagacct gtccagaatg gtgttcaccc ttcggttgct
     2341 caaaggggcc aaagacacgt aaaagctctg aagtatctac catttttgca ttgcgctcac
     2401 cgctacaggt ggacaggtca cctgctttaa ttaacctgtg cagtgttgtt ctactcttac
     2461 ccgtcagtcg ggcagcctca ctaatagaca ctctggccat cagttaccat cctgtaatgc
     2521 atccagtctc gaggacagtg tacggaaaag atgctccgca tcatcgtgga gtttctcaca
     2581 aaggtcggcc tcgacatcga gatcaagctc gttaagcttc tccagcagga gcagagactg
     2641 attctggatg aatttagcca tgttcagcgc tgttttctgt tgtttcgtca taagaggcct
     2701 cagagcgtca gtggtcagtg tagggatctc ctcttttccg cttcctcgct cactcagtcg
     2761 ctacgctcct gccgttcggc tgcggcgagc ggcaggagca tgcgtagaaa cgctcgagaa
     2821 aaccgagtaa tcgcatggag gaaagaagga aaggccgcac agcggccgtt tttccatagg
     2881 ctccgccccc ctgacgagca tcacggaatc tgacgctcaa atcagtggtg gcgaaacccg
     2941 acaggactat aaagatacca ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt
     3001 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     3061 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     3121 cgttcagtcc gactgctgcg cctaatccgg taactatcgt cttgagtcca acccggaaag
     3181 acacgacaaa gcgccactgg cagcagccac tggtaactga tttagggagt tagtcttgaa
     3241 gttatacgcc agttaaagct aaactaaacg aacagttttg gtgactgcgc tccctttaag
     3301 ccagttacct cggttcatag agcgccaact ctactgaacc tttcgaaaaa accgcccgcc
     3361 aaggcggttt ttttcgttca cagccggaag cttacggctg ctgccagata atctcaagaa
     3421 gaaagtaaaa aaaatcgatt aaaacacaag caatcaaagt taatctttga cataaagtct
     3481 agctaatcac aatgaaagct gtacatttta ttaaggtact aacaaatcgt atggcatata
     3541 acaaaaacac gaacagaggc tatcatgata aaggaaataa gtttaaagaa cttcaaatgc
     3601 ttcaatgagc tatatctaaa gcaattaaag actttaaata tcatagctgg gaaaaacaac
     3661 tacgggaaaa catctatact ggatgctatt ttttgttttt atgatgtaaa aaacccggct
     3721 gttttgttaa acattcaagc attcagaaaa gaaatggcag aaataaacaa aaacaagcca
     3781 ttttgggtta gttactttca tgatatggac acatcacaaa agatgtccat agtcattcga
     3841 gatgaacgct ctgaagtaac acaaacatat gaaacagaaa caaatcagag attagaatca
     3901 agtttgtctc taaacgttga caccgtacta tctaaccaaa acatacctcg tcaaactatc
     3961 ggttctcagg ccacagtaaa ctctttaaaa ataaatgtga ccgagacacc caatgcgaaa
     4021 aacagggttt ccaataagct tttctctatg caattgaaag aagatggcgt agaaataaaa
     4081 tccgaaataa aaaacgaaag ggatgggatt aattataaat ttaagacagc aacaataata
     4141 acaacatcca gaaagataaa caaagag
//
