LOCUS       CP052299                5896 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0204 plasmid pE16KP0204-2,
            complete sequence.
ACCESSION   CP052299
VERSION     CP052299.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609929
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:41:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,394
            Genes (coding)                    :: 5,295
            CDSs (with protein)               :: 5,295
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 45 of 99
            Pseudo Genes (internal stop)      :: 22 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0204"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0204-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            258..624
                     /locus_tag="HJX41_27520"
                     /pseudo=""
     CDS             258..624
                     /locus_tag="HJX41_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(876..2297)
                     /locus_tag="HJX41_27525"
     CDS             complement(876..2297)
                     /locus_tag="HJX41_27525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO64845.1"
                     /translation="MKYFSTGFFLKGNVEVKDIINECLSWVSTSPFTSFVPASLDMKRG
                     LDEFDIEVGDERIELINFSDDSIMMSCFRYSKISGAHKWVTDVAVNYYFSEGKTWVQVE
                     SSVVTQQAVHKAPVTKTPLIVLRLLDKFSGGRDDVFKVGIEPVLLDGSEHSLGFACDII
                     NGRHKNRLPVVYVSSKYFFKEHPHNVIPGRLARKLSGLAHVVVEPEGHLFSNKLKHEVN
                     NKNSYGGSVGIYWPGGQSISIHKRGSLSAAEFEKEIFKEIITAMTSLAPLRKNGWSEIV
                     SSKNRKAIQVLKDNGSQSSELASLYEEENESLQDEVKELNQKIASYEARIRVLMERTSM
                     QGGVFLKIGNEYELFEGEVLEIIMSSIRKVLPTNYEGGRYHDVLSSISSANPCSTNLLE
                     RDKKLKQALVGYTKMTPKVRGVLNDVGFSISEEGKHYKITYHGDPRYTYILPKTGSDYR
                     GALNAYSDISKKVFS"
                     /name="hypothetical protein"
     gene            complement(2404..2670)
                     /locus_tag="HJX41_27530"
     CDS             complement(2404..2670)
                     /locus_tag="HJX41_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJO64846.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRHSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2654..2878)
                     /locus_tag="HJX41_27535"
     CDS             complement(2654..2878)
                     /locus_tag="HJX41_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="QJO64847.1"
                     /translation="MLAIRLSDEIESRLNLLAKQTGRTKTFYAREAILTHLDDLEDYYL
                     SAETVARVRRGDEVVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     gene            complement(3953..4375)
                     /locus_tag="HJX41_27540"
     CDS             complement(3953..4375)
                     /locus_tag="HJX41_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO64848.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGPLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4375..4566)
                     /locus_tag="HJX41_27545"
     CDS             complement(4375..4566)
                     /locus_tag="HJX41_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO64849.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(11..190)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.946"
                     /coverage="83.0"
                     /evalue="3.5099999999999995e-46"
     Typing          140..649
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 agacggtcct tcgcgggttt cggggcgcag ccctgaacca gtcacgaagc gctagcggag
       61 tgtatactgg ctcaatatgt tacggcggag taacacttaa taaaagtgct tcatgtgaca
      121 ggggaaaatg cagctacagg gcgtaaggaa gaaaatggga gttacggtga tatgacgcct
      181 cctcgctcac tcggtcgctg ccgctcctgc cgttcggctg cggcgggcgt tacctgcaca
      241 cggataagag caaaggcata cggcatgaca agctgggcaa aaaaaggata cggcgctgcc
      301 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
      361 gcggcgaaac ccgacaggac tataaagatc ccaggcgttt ccccctggta gctccctcgt
      421 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
      481 atctcattcc acgcctgaca ctcagttccg ggaaggcagt tcgctccaag ctggactgta
      541 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat catcttgagt
      601 ccaacccgaa aagacacgac ttaacgccac tggcaacggc cactggtaaa cagaatgtgc
      661 gagagatgta tcgagagttc ttgaagtggt ggccttactg aggctacact ggaaggacag
      721 atttggtgac atgtctcgca caagactgtt accacggtta gagaatctgc caggattaac
      781 taaccttcga aaaaccacct gccagggtgg ttttttcgtt tcaaaactaa gatacatcca
      841 gcaagagatg aactcaagca ccatgtctta ctggtttatg aaaacacctt ttttgatatg
      901 tcggagtaag catttaacgc ccctcgatag tcactccccg ttttaggtaa aatatatgtg
      961 taccggggat ccccatggta agttatttta tagtgcttac cctcttcgct tattgaaaaa
     1021 ccgacatcat tcaaaacacc tctcactttt ggcgtcattt ttgtatagcc tactagcgct
     1081 tgcttcaact ttttatctct ttccaagagg tttgtactgc atgggttagc ggaagatatt
     1141 gaagatagaa catcatgata tctacctccc tcataatttg tcggaagaac cttcctaatt
     1201 gaagacatta ttatttctaa tacctcacct tcaaacaact catactcatt gccaatcttc
     1261 aaaaaaaccc caccctgcat agatgttctt tccatcaata ctcttatcct tgcttcataa
     1321 gaagctatct tttgatttag ctcctttact tcatcttgca gggattcatt ctcttcttca
     1381 tatagagaag cgagttcact actttgactt ccattatctt ttaaaacttg aatggctttt
     1441 ctgtttttac tactgactat ctcagaccac ccatttttcc ttaagggggc cagtgaagtc
     1501 atagcagtta taatctcttt aaatatctct ttctcaaatt cagcagcact aagactacct
     1561 cttttatgaa tactaatact ttgccctccc ggccagtaaa taccaacgct tccaccataa
     1621 gaatttttat tattaacttc atgcttgagt ttattagaaa acaagtggcc ttcaggttct
     1681 acaaccacat gggccaaccc tgatagcttc ctagccaacc tgccaggtat cacattatgc
     1741 ggatgttctt tgaaaaaata tttagagctc acataaacca caggaagcct gtttttgtgc
     1801 cttccattaa ttatatcaca agcaaagcca agagaatgtt ctgaaccatc cagtaaaaca
     1861 ggttcaattc ctactttaaa aacatcatca cgcccaccag agaatttatc tagcagacgc
     1921 aatacaatga gtggtgtttt agttacaggt gccttatgaa cagcttgctg agtaacaaca
     1981 ctagattcaa cttgaaccca ggttttccct tctgaaaaat aataattaac agcaacatcg
     2041 gtgacccatt tatgtgcccc tgatatttta gagtaacgaa aacaagacat cataatcgaa
     2101 tcatctgaaa aatttatcag ttcaattctt tcatcaccca cctcaatatc aaactcatcc
     2161 aaccctcttt tcatatctaa gctagcggga acaaatgaag taaatggtga tgttgacacc
     2221 catgacaaac actcattgat tatatctttt acctcaacat ttcctttcaa aaagaagccc
     2281 gtggaaaaat atttcataaa caccccttat gatttagagt aaaatatttt ttaaaattaa
     2341 aaagccattt tgttaacgac atatttttca cgcctcacat aaaaaaaacc cgcaaagcgg
     2401 gcttcagtcg tagacttcac gtcggtggcc aatggtagca gccagaataa tcagctcgtc
     2461 gtccctgatc tcgcatagga tgcgataatc gccaactcga tagcgccaga attctccaag
     2521 ctcaccttta agcggcttac ctgagtggcg tggatctgcg gcaacggcaa tacgtaaatc
     2581 cataaaatcc acaatccgtc gtgcgttctg cttgtccatc ttacgtaacg acttgagcgc
     2641 tcggtcggaa tagttaatcg tccagaccaa gcgacttcct cacgtcttca gacgaatgta
     2701 caacttcatc cccacggcga acacgtgcca cagtttctgc tgaaagataa taatcttcaa
     2761 gatcatccag atgcgtcagg atcgcttcac gcgcatagaa cgttttagtc ctacctgttt
     2821 gcttcgcaag caagtttaga cgggactcaa tctcgtcaga caatctgata gccaacataa
     2881 agacctctct gaatccaaat tgatatacat gtataacaaa attatacaac attaaaaatc
     2941 aaagaacaaa aaacaatctt tccattcatc ttgactacct acaactgtcg atatatgcag
     3001 ttatagagtt tcctcttaac acgatgaagg atatccatta tggctcttaa acctggacca
     3061 aaacgaattg cagaatcaac agggcagcct gaccagcgcc aacgcgacaa taaaaaaact
     3121 cccggaaata ctgacaagtt aaaaccaagt aaatcttcag gaaagtagtt gttagcaaag
     3181 cccttagggg ctttttttgt acttgtcggt cagacggatt aaacataaca tccgttgcgt
     3241 gcaccgcctg agaggctctc agggcgtttc tgacgggaaa ccggtaaaac aggcaacaaa
     3301 ccacccgaaa acccgccaga aacgcgcagg ctctgttttt acggcatgca tgagtatgcg
     3361 cgcaagtgca tagcgcggaa attcacgcca ttcagccggg gtaagtcctg tttccgatgt
     3421 tcttcttacc cgaaaacggt gaacggggac gcaatttatt gcgtggccgt gagacgttgc
     3481 cggcacagcc ggaaaccccg tatcagcacg atcaggaatc gggttcgtcg acggccggca
     3541 atcacgttat ggcaagtcgc cggacgactc accacacgat tcaccggcga ttcgactcgt
     3601 gacttgactc tagagtcacc gcggtcaaaa aaacgccaaa gcgacgattc gactctagag
     3661 tcaagtcgtc ataagttact gatagtgtgg agtgagtcgt cgatgactct atgacgaatc
     3721 gtcggcaagt cgtacacgaa tcaccgacga cttggattga ccttacagaa ggtaatactg
     3781 cgtaatttaa taacaaccaa ctgactgata ttaatgagtt atctgcaaaa atttaatttt
     3841 gcgtggggtg tggatatttt tagtgtgagg agcgttcaga gatctggcga tttttgggaa
     3901 ggttcacgaa cgccccggca tggccggaga ggaaaactca agcatggcgg gatcaggatt
     3961 ttttccagaa tttccaccac ggggaatggg gtggtgaaga aggttcgtgc ttgtgctcca
     4021 gaagctgcat agcctgacga agggaatcaa tatgctcatc tcgagcctta acagcccctt
     4081 taagaccatc gttttcagct tcaagtaaag cgattctatg ttgtaaatca tgtaaaccaa
     4141 cgctgttaat acctgtagat tctgcgtcac tttttacatc atattttaca gcatcgatta
     4201 caccatcaag aggaccaaat actctaataa gctctgatgt atcaataact ttaataccct
     4261 tcccgtgctt tacaacagag gtgtaactta acttaccact gttaatcatc ttgtaaatag
     4321 tagggcgact aaccttaaca agccgtgctg cttcactaat agacactctg gccatcagtt
     4381 accatcctgt aatgcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc
     4441 gtggagtttc tcacaaaggt cggcctcagc atcgagatca agctcgttaa gcttctccag
     4501 caggagcaga gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt
     4561 cgtcataagg gacctcagag catcagtgct cagtgtaaaa ccggtaaggg ggtcggatgt
     4621 acgaatggtt cagagaacgg atctgaggta atcctgtagt tattttgtag tttatctgta
     4681 gtcatcgcac taaaaatcag gattaagtca tcagcttttg tagtctttct gtagtttacc
     4741 tgtagtccgg aaaaataatc cgttcatgcg agcatgaacg aggtcgttaa tgacgttttt
     4801 ttgtcaccga cgccccttaa cctccgcaga tcacccggcc agaatgataa ttttttggcc
     4861 cttaaatggt catacaccta acgcagctgc gattttcatc gcaactgcat gaattaaaag
     4921 gtaaaaactg aaatctccgt ttatttatgt aatacgcaac gtattacaat cacctgtaat
     4981 cgcatgaaaa taaagaaata aaatgtaata ccggcgtatt accacgtacg cgggcaagcg
     5041 aaaagctata acgcttcccg gccgacaggc ggcccggaat ccacaggggc atgcaggttc
     5101 tcttcagtaa cctgcgccat taaatcaaaa aggtcatatt cgccgggaag ctgcgtatca
     5161 ggggtcagtt tggatataga gaattattgt acggtaagcc tgttttttga acagacttac
     5221 ctatagcaat agggtgtact cccgcgctat acggcatttt cagaggtttg accttaatcg
     5281 gataacctaa atgcgatact gcaagagcga caacttaaaa gcgatagcca ctgacgcaaa
     5341 atcaacccct tacgaaccaa gattggttaa ataatgcgct taacgtacaa aaatttccga
     5401 tctccaaact gacccctact ccgaaagcgc agtagaaaaa aggcaaaacc tgctctggag
     5461 tacagtcaga agaacggaaa aaaaccgggc ggctagcccg gaaggtaagt caagttagat
     5521 tggaacaatc ttccgtgaca taggacatgt cacaacgacc atcatccatg acggccgcct
     5581 gacacacagt atggttcaaa aatcagaaaa aaaactcaga cggtgacata tttaatacga
     5641 acagattcaa ggaacgccaa catgcactcc ctgacccggg gatctgtctc agataagacc
     5701 agatagtaag catccagcat cattttgccg gagtctcccc cagactggcg taagcagtca
     5761 gaaatcactg acacagccag ctgccgttca gcatcactca tattcatcag gataccagag
     5821 gaaaaatcaa acaaggaaac ctcagttaac ggcagccgaa agaaaatatt gacagtttag
     5881 cagtaaaaaa acaaaa
//
