LOCUS       CP052294                4976 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0212 plasmid pE16KP0212-2,
            complete sequence.
ACCESSION   CP052294
VERSION     CP052294.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609931
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:41:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,512
            CDSs (total)                      :: 5,390
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 48 of 113
            Pseudo Genes (incomplete)         :: 59 of 113
            Pseudo Genes (internal stop)      :: 22 of 113
            Pseudo Genes (multiple problems)  :: 14 of 113
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0212"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0212-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(4961..4976,1..584)
                     /locus_tag="HJX43_27535"
                     /pseudo=""
     CDS             join(4961..4976,1..584)
                     /locus_tag="HJX43_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020282000.1"
                     /note="internal stop; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /name="Txe/YoeB family addiction module toxin"
     gene            844..1746
                     /locus_tag="HJX43_27540"
     CDS             844..1746
                     /locus_tag="HJX43_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="QJK15202.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSAIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKKMVE"
                     /name="replicase"
     gene            1748..2455
                     /locus_tag="HJX43_27545"
     CDS             1748..2455
                     /locus_tag="HJX43_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK15203.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(2492..3106)
                     /locus_tag="HJX43_27550"
     CDS             complement(2492..3106)
                     /locus_tag="HJX43_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="QJK15204.1"
                     /translation="MGKHKVFISYHHANDQYYKNAIEKMNEQHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDKYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGAYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            3495..3896
                     /locus_tag="HJX43_27555"
     CDS             3495..3896
                     /locus_tag="HJX43_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QJK15205.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEQHSEQVFEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAAWWKFWKKS"
                     /name="DNA-binding protein"
     gene            complement(4673..4834)
                     /locus_tag="HJX43_27560"
     CDS             complement(4673..4834)
                     /locus_tag="HJX43_27560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJK15206.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
ORIGIN
        1 agctgatttg gttgtgggtt gttagggaaa tatctatatg tacttatttg tgtacatgtt
       61 ctatgctaag ccccaacaaa tgggggttaa caacatgcat gcaatcaatt ttactgatgc
      121 ccgtaaacat tttgccgaga cgatgaagcg tgtcactgat gatgctgaac cggtacgtgt
      181 catgcgccgt gatgcgcctg atattgttat gatcgatgcg ggagagtatg aggcgctaat
      241 cgaaactgtc tatctgttca gcaatccggc gaacgcagag catatcaatg agtcaatgaa
      301 acaggcggag cgcggcgaat ttgtcgaagt ggaatattaa gttcactagg aatggcgctg
      361 atgaccttaa ctattggcgt gatactgaca aaaagaagtt tgagcgtatc cggcaactgt
      421 tgctcagcat cgaggctgat ccgctgacag gtataggaaa acccgaacgg ctgcggcacc
      481 acaaagatcc ggcactctac tcccgcagga ttgatcagag ccaccgtctg gtttattcaa
      541 tccagaacgg agaaatcatc atctacgcag cccgttatca ctatggtgac aaatagcaaa
      601 agtcgatata gcccgcttga tgcgggcttt tcatgcaagg cgtacaacgg atgttagggt
      661 aagtcgctgt cagatcgaca caaaggcaaa gtcggtgtaa cgttagcctc agaaaaaaca
      721 aagccccgga aatcatgtca tatcttggcg gatcgcatga taaccagggc taggtcgaaa
      781 ccttgaaagg atattagcac atgaaccgtg ccagacaaca gcccgccata agcgcagggc
      841 acattgcata acgccgcact cgaacttttc aacgaacgct taccacacaa gccgtacttt
      901 tcggacgatc tgcactttgg tgtccgtatc gccggtaagg aacgcgctat tctggcgaaa
      961 tatatccagt tcaatcagcc tcacgccatg ttctggctgg gatttgacgt tgaccgtgcc
     1021 ggttccgcca tcgactggag cgaccgcaac gcgcctgcac cgacgctgac cataactaac
     1081 cccgaaaacg gacacgcgca cctgctgtac gcgctggaaa actcgatacg caccgcgccg
     1141 gacggcaaaa taaaaccgtt acgctacgcc gcctccgtgg agaatgcgct acgtaaaaaa
     1201 ttagaggctg acgctggata ttcggggctc atctgcaaaa atccgaacca caaccactgg
     1261 aaaatcgccg tgtggcagcc gaagctctac actctggact ggctggccga ctttctcgat
     1321 ctgaacacag ggaatgacaa ggaaatcgtt gccgactacg gcttaggccg taactgcacg
     1381 ttgttcgaca aaacccgtaa gtgggcatat cgcgccatcc gtcagggctg gcctgagtat
     1441 gagcagtggc tgcaagcctg ctacgaacgc gcttgcgcat acaacctgca atttgcacga
     1501 cctctggatg aaaatgaggt aagagggata gctaagagca ttgctaaatg gacatcaaat
     1561 aaattttcac ctgaagagtt cagtaaattt gttgatatta ctcattcctc tgaaattcaa
     1621 tcaaagagag ggaaaaagtc aggacagtca aggaggaaag gaagcctaga ggaaataaaa
     1681 ccttgggtag ctatgggaat aagcaggcgg aagtattttt atatcaagaa aaaaatggtg
     1741 gaataagatg aaaaccttcg taactctttg gactatagtt tttctagcat ttattgccgt
     1801 gatgtgtact gttgatatat ctaattacat tccgtctgtt tacacagttt ttagtaagaa
     1861 tagacctctt gtaactggcg ccctttctat tcttttgata tctgttttta tttggctgat
     1921 ggtatccctc tacttactta aaaagtattc ggttaaggtt gagaaactta gccttggtgg
     1981 agtgaatata cttttcaatg agagtgctac actttacaaa aagagtgtaa aaaatcacct
     2041 agattcaaaa agagcaattt tcaaattaag aaaaaacgtt gatgcctttg atgaggttat
     2101 ttcatcatat tatcagactt accaatttat tcgtgatgaa atgaaaatac taaaccctaa
     2161 aaaagatcgt gagatttatg atttcagcaa tgagatgctc atggtgttaa atagatttct
     2221 aactaactac cagaacaact acaagcgttg gtataagtac atatcagaca aggatgaggt
     2281 tgttgatagg gttagcggtg atgtcatgag atttcatgca accagcataa ataaaataca
     2341 aatgcagtat tatgattatg agcgtatttt aaaaggtttt aaagatgtta accggttctt
     2401 tgttaatgat gtgaagccta agtttggaat caatacagat aagtggaact ggtaaattaa
     2461 aggagtagcc aaggctactc cttggatagt actatgaatt atgcattctc atttctctcg
     2521 acatatcata cgttgctttt tctctatcgt tataagtcat gtctattaat tttctgagtg
     2581 tttctgggtt attttctatt tgattccagt tgaccactga tatatacgcc ccgctcttca
     2641 tgaagttgtc tattattctt gcaggcatat atgggaacct ttcctcataa gttgagcgtt
     2701 tatcaataga aaaccattca gttacggtag gatgaacttc tgatttttca ttagttccat
     2761 gtgttgaacg aatgtacgta gtatttgttg acgggagatt gacgactaaa attcctgatt
     2821 ttttgttaat ggtactatct ctcatgctag agtaaagttc ccaatctacg tgttttctgt
     2881 tttttgtttc agttccaaca agaaggataa gaactgatgt atctctaaga tatttatctc
     2941 ttattatctc tcttattttt tgtggagctt catcttcatc tatatcacct aaagatactg
     3001 agcgattcac gaatatttca tgttgctcat tcattttttc aattgcgttt ttgtagtact
     3061 gatcattcgc atggtggtag ctaatgaata ccttatgctt tcccattttt attgtccttt
     3121 ttttgtttgt gtgcactcta ttttgcactt gaacctatat cagataacag ctctcttaga
     3181 gagctgtttt tttcagggtc ccttcagcaa ggtctcacca tgactggttt agggagggag
     3241 ggctctttgc ttccctaaac gccttgagaa gagatctata acctcacttc attcgtacgt
     3301 accgctcgcc gcagtctcat gaccgaacgt agtgagcgaa tgcgcgagga agcggaaagt
     3361 catctgttgc ctgcattttg cacggtcact cctgtgtttc atcgcccttc cgaaaaacac
     3421 atcacttctg gaccggtatg tcgtaagctg gcccctgttt ccttgagact gaatgcatta
     3481 aaggatgata actgatgacc agagtgtcta ttagtgaggc tgcacggctg acgggtaaga
     3541 gccggacaac gctgcacagg ttaatcaaaa caggtgagct gtccacttgt agcggtgaac
     3601 gaaatgcaaa aatggtagat acttcagagc ttttacgtgt ttttggtccc tttgagcaac
     3661 ctaagggtga acagcattct gaacaggtat ttgaacagcg tgatacagct tctgctgacc
     3721 agaatgaaca agtgattcgt catctgaaac aggagattga acacctgaac accttacttt
     3781 tgtccaaaga ttcgcacatt gacagcttga aacaagccat gctccttctt gaaaatcacc
     3841 aggccaaatt aactgaatcc ccggcggcat ggtggaagtt ctggaaaaaa tcctgatccc
     3901 gcgatgcttc agttttcctc tctggccatc ccggggcgtt cctgaacctt cccagatacc
     3961 gccagatctc tgaacgctcc tcaccactaa aaatacccac accccacgca aaattaaaat
     4021 tttgcagata actcattaat atcagttagt tggttgttat taaattacgc agtattacct
     4081 tctataaggt caatccaagt cgtcggcgat tcgtgtacga cttgccgacg actcgtcata
     4141 gagtcatcga cgactcactc caaactatca gtaacttatg acgacttgac tctagagtcg
     4201 aatcgtcgct ttggcgtttt tttgaccgcg gtgactctag agtcaagtca tgagtcgaat
     4261 cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca taacgtgatt gccggccgtc
     4321 gacgaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc
     4381 ccgttcaccg ttttctgagg agaagaacgc cggaaacagg acttcccctg gctgaatggt
     4441 gtcaatttcc gcgctatgca cttgtgcgca tactcatgca tgccgtaaaa acagagcctg
     4501 tgcgtttctg gcgggttttc gggtggtttg ttgcctgttt tgccggtttc ccgtcagaaa
     4561 cgctctgagg gcctctgagg cggtgcgggc aacggatgtt atggtaaatc aagtgaatgg
     4621 caggtacaaa aaaagcccca gagggctttt agtttagact tctgaagata ttttattttt
     4681 cttggaagtg gtggttgttt ttgctcggag ggaacttatc acccttgctc acatggcgat
     4741 catattcaga accagcgtat gagttgtcgg tatcatgata ttgaccgtaa gtacctgttt
     4801 ttggacattc ttgcccagac caatgttttg ccatttactt ttcctttgct cgttttagga
     4861 tctaagctcc tagcggtacg aaattgtgcc gcacttcata aatgtaattg cttgaaatta
     4921 tgcttcaatg tattgacttg ttgtttttgt ggttattcga ttgagttcac attttg
//
