LOCUS       CP052291                3484 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0218 plasmid pE16KP0218-5,
            complete sequence.
ACCESSION   CP052291
VERSION     CP052291.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609932
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:42:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,713
            Genes (coding)                    :: 5,558
            CDSs (with protein)               :: 5,558
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 77 of 155
            Pseudo Genes (incomplete)         :: 87 of 155
            Pseudo Genes (internal stop)      :: 33 of 155
            Pseudo Genes (multiple problems)  :: 31 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0218"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0218-5"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(2644..3484,1..692))
                     /locus_tag="HJX44_29175"
     CDS             complement(join(2644..3484,1..692))
                     /locus_tag="HJX44_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="QJO75785.1"
                     /translation="MKKSVVRQVLEMSGPCISSDLAERIQWQHPSMSPEAIRKMISRST
                     DIGKLPFLKFSHNRRFIYLKDDFGSFKFWRALEKCMYEANSTYSHAILAVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYETVLRNLLSAKILRAVYIDGVGDCVLINNNIANDVNIRTM
                     ANCESFFDKPILELVKAWLRNLGLVAFNQIKTKYDGEGNPVVGSFEWDMTAPSYVSPLA
                     EYVGGKLMPGFVACDFSLGFNRDEITTAAAETFIRKVQMTKSSRASQRIMFVIFARRFG
                     KIAFNKLRSEGVLAVTIANAFGNKVDESLTRLSRVVQGSLSIEKHPDELLQMVKDLESV
                     SGENGNLRGYIFELFVSSQISNFYGLGNIYINREYKINGKHAEADVVLESSDDIYIIEC
                     KNVKVLPSTELTRWMKERVPTINSYYKINNLEKKNIHHYLWVTGNVYNNDLKRLDSFKK
                     NNKSIDVDYLFGEHLDDFFYNKKRVFNLYRKVISPDYKKGVMPDFELEDDFF"
                     /name="NERD domain-containing protein"
     gene            complement(1688..1879)
                     /locus_tag="HJX44_29180"
     CDS             complement(1688..1879)
                     /locus_tag="HJX44_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO75786.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2297..2455
                     /locus_tag="HJX44_29185"
     CDS             2297..2455
                     /locus_tag="HJX44_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJO75787.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKTPLAAAIGIWFTERRGV
                     YKHTQSS"
                     /name="replication initiation protein"
     Typing          2117..2596
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.758"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ggcatcagtt ttccgccgac atattcagcg agaggggaaa cgtaacttgg agcggtcata
       61 tcccattcaa aagagcctac aactggattt ccctccccat cgtattttgt ttttatctga
      121 ttaaaagcga ctaggcctaa attcctcaac caagctttaa caagctccaa tattggtttg
      181 tcaaaaaagg attcgcaatt tgccattgtt ctaatattaa catcgttggc tatgttgtta
      241 tttatcagta cgcaatcacc aactccatca atatacacag ctctaagtat ttttgccgaa
      301 agtagatttc ttagaactgt ttcataactt aaatgtttag cttgttttat aggagatcca
      361 gacacaatcc caaaatcttt caccttcaaa taaccgccat tatttattac ggcaagtatc
      421 gcgtgtgagt aagttgagtt tgcttcgtac atgcactttt cgagagctcg ccaaaatttg
      481 aaactgccaa aatcatcttt tagataaatg aatctcctgt tatggctgaa tttcaaaaat
      541 ggcagcttac ctatgtccgt gcttcgcgag atcattttac gtatcgcttc aggggacatt
      601 gagggatgtt gccactgaat tctctctgca agatctgaag agatgcatgg cccgctcatt
      661 tcaagcactt gtctaacaac actttttttc atagcctcac cttatttctg tcacactacg
      721 ctatttataa cattacacta cgtgtaataa cgcgcagtct ttttaaaaaa aatgactata
      781 aaaaacaatt gaaaatcaga tagttaatta cttaatttaa attaagcaca aagaaatatc
      841 gtctttataa ttatttaatc cttttaaatc ataaagatag ctttgtgaca tttttttatc
      901 gaactatcag ctactccatt taccataaca tccgttatca gcaccgcctc agaggccctc
      961 agggcgtttc tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga
     1021 aacgcgcagg ctctgttttt acggcatgca tgagtatgcg cacaagtgca tagcgcggaa
     1081 attcacgcca ttcagccggg gtaagtcctg tttccgatgt tcttcttacc cgaaaacggt
     1141 gaacggggac gcaatttatt gcgtggccgt gaggcgttgc cggcgcagcc ggaaaacccg
     1201 ttttcagcac gttcaggaag ggggtgatgt acaaatggca cagaggatgg aagtgacgta
     1261 tgagctgaag gaatgatgta aaagtgatgt aaaagtgatg caaaagtgtt gtaaaagtaa
     1321 tgtacagagc gaaggagtgt tgtaaaagtg ttgtaaaagt gatgtatgac ctgaaggagc
     1381 gatgtaattc tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag
     1441 gaacgctttt tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt
     1501 atttttcaac ctcaaaatgg tcatacacct aacgcagctg cgattttcat cgcaacttca
     1561 tgaaaaataa ataaaaaaga aaaaatacag ggattctggg tattaccggg gtaataatat
     1621 taagcgctaa cacaatgaaa attaaggcga tgccggtatt actccggtaa tacccagaac
     1681 ggagccatta cgcaccgtcc tgctgctcat tcaggcgcgc tgacaggctt cggtaaaggc
     1741 gttccgcatc ctcatggagt ttctcgcaga aatcagcctc atgatccaga tccagctcat
     1801 taagcctgtc cagaagaagc agggactgat cctggatgaa tttagccatg ttaacggcag
     1861 tcttttgttg tttgttcatg aaggccccag cgcgaaagtg cttactgtac gttctgatgg
     1921 ttttccgctt cctcgctcat tcgctcactc cgttcggtca tgagtctgcg gcgagcggag
     1981 ggaagataag caggtaacgt aacggtaagc ataacgaaag ctaaggacaa gggcgaaaag
     2041 gccacgcccc ggcggggcgt ttttccatag gctccgcccc cctgacaagc atcaagaaat
     2101 ctgacgctca aatcagtggt ggcgaaaccc gacaggactt aaagataccg ggcgtttccc
     2161 cccggtggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcactccg
     2221 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     2281 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttatccgg
     2341 taactatcgt cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
     2401 tggtatctgg tttacggagc ggagaggcgt gtacaagcat acacagagtt cttgaagtgt
     2461 tgacctgact acggctacac tggaaggaca gatttggtca ctgcgctccg tttaagccag
     2521 ttaccacggt tcagaaactt gccggtttcc tgaaccttcg aaaaaccacc ttgccgggtg
     2581 gttttttcgt ttcagagatg gaagatacgc ctttcaaagc gccatcgtca agaaacgcag
     2641 tagctagaaa aaatcgtcct ctaactcaaa atcaggcatt acgccttttt tataatcagg
     2701 tgaaatgact tttctatata agttgaacac ccgcttttta ttgtaaaaga aatcatccaa
     2761 atgctcacca aacaaataat caacatcgat actcttatta tttttcttaa aagagtctaa
     2821 cctttttaaa tcgttgttgt acacattacc tgtaacccac aaataatggt ggatattctt
     2881 tttttctaaa ttatttattt tatagtaaga gtttatagtt ggaacccttt ctttcatcca
     2941 acgcgtaagt tcagttgaag gtaagacctt aacattcttg cactctatta tataaatatc
     3001 gtcactactc tctaatacaa catcagcttc tgcatgctta ccatttatct tatactctct
     3061 attaatgtaa atattaccaa gaccataaaa attagaaatt tgcgaggata caaaaagctc
     3121 aaaaatatac cctctcaaat tgccattctc tccagaaaca gactcaagat ctttaaccat
     3181 ctgaagcaat tcatctggat gcttttcaat ggacaaagaa ccctgaacca ccctcgacaa
     3241 cctggttaag ctttcatcaa ctttattacc aaacgcattt gctatagtta cagccagaac
     3301 cccctcactt ctcaacttat tgaaggctat tttgccgaaa cgccttgcga aaataacaaa
     3361 cattatcctc tgactcgctc gagaagactt ggtcatctgg acttttctaa tgaatgtttc
     3421 tgctgcagcg gtagtaattt catcacgatt aaagccgaga gaaaaatcac aggcaacgaa
     3481 acca
//
