LOCUS       CP052290                4167 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0218 plasmid pE16KP0218-4,
            complete sequence.
ACCESSION   CP052290
VERSION     CP052290.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609932
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:42:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,713
            Genes (coding)                    :: 5,558
            CDSs (with protein)               :: 5,558
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 77 of 155
            Pseudo Genes (incomplete)         :: 87 of 155
            Pseudo Genes (internal stop)      :: 33 of 155
            Pseudo Genes (multiple problems)  :: 31 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0218"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0218-4"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(3717..4167,1..182)
                     /locus_tag="HJX44_29140"
     CDS             join(3717..4167,1..182)
                     /locus_tag="HJX44_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO75780.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="hypothetical protein"
     gene            548..790
                     /locus_tag="HJX44_29145"
     CDS             548..790
                     /locus_tag="HJX44_29145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO75781.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1136..1537)
                     /locus_tag="HJX44_29150"
     CDS             complement(1136..1537)
                     /locus_tag="HJX44_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO75782.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1537..1728)
                     /locus_tag="HJX44_29155"
     CDS             complement(1537..1728)
                     /locus_tag="HJX44_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO75783.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1685..2119
                     /locus_tag="HJX44_29160"
                     /pseudo=""
     CDS             1685..2119
                     /locus_tag="HJX44_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            2144..2257
                     /locus_tag="HJX44_29165"
                     /pseudo=""
     CDS             2144..2257
                     /locus_tag="HJX44_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2602..3717
                     /locus_tag="HJX44_29170"
     CDS             2602..3717
                     /locus_tag="HJX44_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QJO75784.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     Typing          1850..2257
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 acatctcagc gagtgacaaa gagtacactt atccaacaaa cgaaaaaaaa gctaaggtac
       61 aagtctatct tagctgcaat agagaatccg actcaagaat aggaatatcc atgaaaaatg
      121 gaataataaa cacagcagac aattgctttg atgaaataaa agattttatt tcaaaaatat
      181 aaatccaaat caagccagcc cttcacgggc tggcttatca ccagggtgtt attaaacgcc
      241 ggataacgta tagaaagcat catacacttt agttatcagc aatctcaaac gtcctaaaga
      301 catcaccaga tgccaaaaca atgattgtgc agatctaatg taaaagaccc agcacaagat
      361 gctcccttaa gttatattct ctctattcac ttaaaacaat ttaacataaa atcataattg
      421 tacgcaccgc taaaacggac tcaggacgtt tctgacggga aaccggtaaa acaggcaaca
      481 aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg
      541 cgcgcaagtg catagcgcgg aaattcacac cattcagcca ggggaagtcc tgtttccggc
      601 gttcttctcc tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt
      661 gccggcgcag ccggaaaacc ggttttcagc accttcagga atcgggttcg tcgacggccg
      721 gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg cgattcgaat
      781 cgtgaattga atcaagagtc accatgagca aaaaacaccc aaagtgatga attgaatcaa
      841 gagtcgattc atcataacaa tatgataata aagcgtgatt cacatatgaa tcgtgggcaa
      901 ttcgtcagcg attcgccata gagtcgtcca cgacttggat ctgcactttg taacacatca
      961 ccgcacattt atttttcatt aacccattgt tgtaaataag gaatctgcaa aattttaatt
     1021 ttgcgtgggg tgtgggtatt tttagtggtg aggagcgttc agagatctga cgatttttgg
     1081 gaaggttcag gaactccctg ggatggccgg agaggaaaac tgaagcatga cggggtcagg
     1141 attttttcca gaatttccac catggcgccg gggattcagt taattttgcc tggtgatttt
     1201 caagaaggag catggcctgt ttcaggctgt caatgtgcga atccttggac aaaagtaagg
     1261 tgttcaggtg ttcaatctcc tgtttcagat gacggatcac ctgttcactc tggtcagcag
     1321 aggctgtatc acgctgtcca gagacctgtc cagaatggtg ttcacccttc ggttgctcaa
     1381 aggggccaaa gacacgtaaa agctctgaag tatctaccat ttttgcattg cgctcaccgc
     1441 tacaggtgga caggtcacct gctttaatta acctgtgcag tgttgttcta ctcttacccg
     1501 tcagtcgggc agcctcacta atagacactc tggccatcag ttaccatcct gtaatgcatc
     1561 cagtctcgag gacagtgtac ggaaaagatg ctccgcatca tcgtggagtt tctcacaaag
     1621 gtcggcctcg acatcgagat caagctcgtt aagcttctcc agcaggagca gagactgatt
     1681 ctggatgaat ttagccatgt tcagcgctgt tttctgttgt ttcgtcataa gaggcctcag
     1741 agcgtcagtg gtcagtgtag ggatctcctc ttttccgctt cctcgctcac tcagtcgcta
     1801 cgctcctgcc gttcggctgc ggcgagcggc aggagcatgc gtagaaacgc tcgagaaaac
     1861 cgagtaatcg catggaggaa agaaggaaag gccgcacagc ggccgttttt ccataggctc
     1921 cgcccccctg acgagcatca cggaatctga cgctcaaatc agtggtggcg aaacccgaca
     1981 ggactataaa gataccaggc gtttccccct gatggctccc tcgtgcgctc tcctgttcct
     2041 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     2101 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     2161 tcagtccgac tgctgcgcct aatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     2221 cgacaaagcg ccactggcag cagccactgg taactgattt agggagttag tcttgaagtt
     2281 atacgccagt taaagctaaa ctaaacgaac agttttggtg actgcgctcc ctttaagcca
     2341 gttacctcgg ttcatagagc gccaactcta ctgaaccttt cgaaaaaacc gcccgccaag
     2401 gcggtttttt tcgttcacag ccggaagctt acggctgctg ccagataatc tcaagaagaa
     2461 agtaaaaaaa atcgattaaa acacaagcaa tcaaagttaa tctttgacat aaagtctagc
     2521 taatcacaat gaaagctgta cattttatta aggtactaac aaatcgtatg gcatataaca
     2581 aaaacacgaa cagaggctat catgataaag gaaataagtt taaagaactt caaatgcttc
     2641 aatgagctat atctaaagca attaaagact ttaaatatca tagctgggaa aaacaactac
     2701 gggaaaacat ctatactgga tgctattttt tgtttttatg atgtaaaaaa cccggctgtt
     2761 ttgttaaaca ttcaagcatt cagaaaagaa atggcagaaa taaacaaaaa caagccattt
     2821 tgggttagtt actttcatga tatggacaca tcacaaaaga tgtccatagt cattcgagat
     2881 gaacgctctg aagtaacaca aacatatgaa acagaaacaa atcagagatt agaatcaagt
     2941 ttgtctctaa acgttgacac cgtactatct aaccaaaaca tacctcgtca aactatcggt
     3001 tctcaggcca cagtaaactc tttaaaaata aatgtgaccg agacacccaa tgcgaaaaac
     3061 agggtttcca ataagctttt ctctatgcaa ttgaaagaag atggcgtaga aataaaatcc
     3121 gaaataaaaa acgaaaggga tgggattaat tataaattta agacagcaac aataataaca
     3181 acatccagaa agataaacaa agaggcaaca attacaaatg tatctagcct actaacacaa
     3241 aaaaggaaaa aagacatact agaaaactta aaaaaaatag atgacagaat tgtagatata
     3301 gcaatatctg cgataggtaa caataaggaa atatatttag acattggatt tagtgagcta
     3361 aatgaaatat caatgcttgg cgagggtatc agtcgagcat tatcttttat cagctcagtg
     3421 ctggtacagg aaaactcaat tattcttatt gatgaaatcg agaacggaat acattactca
     3481 gtaattaaag acataataaa atcattaatt tcatctgcaa aacaaaacaa caatcaaata
     3541 tttgcaacaa ctcatagtca agatgtgata cgagcaataa acgaaataga tagcaaaaat
     3601 gaagatatcg cttatattag actaggaaga gaaaagaact ccctaaaacc aacagccgta
     3661 caattcaata tggatgattt ctcctactca gttgagaacg actgggaggt aagataatga
     3721 aagacataaa taatagcaaa aagatcattt tcgttgaggg tgatgatgat tttcattttc
     3781 tatgcaacct acttgaaata caaggcatta ccgatgtttt tgtcgaaaaa ataaatggaa
     3841 aatcaaaatt aaaacaagca cttagagcat tcaaaaacat tgattctttc gatgaaaaag
     3901 aaagtatttt tattatatta gatgctgaca catcattttc tgacacagaa agatcaataa
     3961 ttgccacatt gcgagaacta caaatctcct cccccagctc gcataacgag atagctatga
     4021 atggcaatac aaaaatttct ttctttataa tgccaggaaa agacaagcaa ggtgccattg
     4081 aagatctgat aattgaacac gcaagcactc gagcgttata tgaatacata atagaatttt
     4141 tcgaaaaaat taaaaataat aaagatg
//
